FastQCFastQC Report
Sat 31 Dec 2022
SRR5845675.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845675.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences219866
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGC14400.6549443752103554No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGCCGTCT13060.5939981625171695No Hit
TCTCACGGGCAAAGTGGTAGCTTTCTGTAAGATTTGTATTTTGAGATATTCT3340.15191070925017966No Hit
ACATAGGGGGAGCACTGAGAAACCTGGCTGTGGATGCTCGCAACAAAGAATT3080.14008532469777046No Hit
ATGCAAGGGAACCTAAGATGTATTGGTTATGGAATCATGTGTTGGATAGGTT2940.1337178099387809No Hit
TGCGATGGGCAGGAATGTCTGGAGTGGTAACAGCTCTCTTCTTACTCATTAC2580.117344200558522No Hit
GCTGGACGGGATGCCTGGCTCATAGATGAAATCCCTTAAGCAGGATTGAAGA2520.1146152656618122No Hit
TGTCGTAGGGGCAGTGAATGTGGAAAATTCTATAGCCACAAGTCCAACCTTA2480.11279597573067232No Hit
CGGTCAGGGCTGCTTGATTCTTTTAAACCATTTCAATCACTTTGTGAAATTT2460.11188633076510239No Hit
GCGTAGGGGTCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTC2370.10779292842003765No Hit
AAGCATGGGAAGATGGTGATGGAACTATAACAACAAAGGAATTGGGAACTGT2270.10324470359218797No Hit
AGAGGTGGGACTAAACCAAAGCATAAATGCAATTTTTCCTGAGTCTTTGAGT2250.10233505862661803No Hit
CAAAACGGGTACTGCTACGGCCCCCAAGGCAGCAGGAGCCCATACATCCCCC2200.1000609462126932No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)