FastQCFastQC Report
Sat 31 Dec 2022
SRR5845674.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845674.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences221718
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGC13720.6188040664267223No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGCCGTCT13040.5881344771286048No Hit
TCTCACGGGCAAAGTGGTAGCTTTCTGTAAGATTTGTATTTTGAGATATTCT3530.15921125032699193No Hit
ACATAGGGGGAGCACTGAGAAACCTGGCTGTGGATGCTCGCAACAAAGAATT2950.1330518947491859No Hit
GCTGGACGGGATGCCTGGCTCATAGATGAAATCCCTTAAGCAGGATTGAAGA2900.1307967778890302No Hit
GCAGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2570.11591300661200263No Hit
GCGTAGGGGTCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTC2530.11410891312387808No Hit
AGAGGTGGGACTAAACCAAAGCATAAATGCAATTTTTCCTGAGTCTTTGAGT2370.10689253917137986No Hit
TGCGATGGGCAGGAATGTCTGGAGTGGTAACAGCTCTCTTCTTACTCATTAC2350.10599049242731758No Hit
ATGCAAGGGAACCTAAGATGTATTGGTTATGGAATCATGTGTTGGATAGGTT2310.10418639893919303No Hit
CACTCAGGGAACAGAGAGAAGGGACTTCTCCTAAAATTCAGAGAGCCCTTGC2300.1037353755671619No Hit
TGCTAGGGGACCTTTGAGGACAACCTGAGGTCACTGCACTTGCAGGAGGGCC2290.10328435219513075No Hit
GCTTTCGGGATAGGCAAAAATGATTTCTAGATGTGGAAAGACTTTCTAAGTA2260.10193128207903733No Hit
ATGCTCGGGGGAAGAAGTAAAGAAGAGGTCACTAGATGTTGCAGTGAAAAAT2250.10148025870700621No Hit
TAACATGGGAATTCAAATATGTGTTGTTCAAGTGTCAGCTGTATTGAAAATA2230.10057821196294392No Hit
AACTAAGGGAGGTACTCACTAGACTGGTACCTTCTGCCACCATGGGGGAGCT2220.10012718859091277No Hit
GTCGCCGGGAATGAATGAATTAATGGGAAAGAGATTTTCTCCAAAGGACCCG2220.10012718859091277No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)