FastQCFastQC Report
Sat 31 Dec 2022
SRR5845673.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845673.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences217680
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGC13110.6022601984564498No Hit
CTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGCCGTCT12960.5953693495038589No Hit
TCTCACGGGCAAAGTGGTAGCTTTCTGTAAGATTTGTATTTTGAGATATTCT3130.14378904814406468No Hit
ATGCAAGGGAACCTAAGATGTATTGGTTATGGAATCATGTGTTGGATAGGTT2920.13414185961043734No Hit
ACATAGGGGGAGCACTGAGAAACCTGGCTGTGGATGCTCGCAACAAAGAATT2650.12173833149577362No Hit
GCGTAGGGGTCCCCACCCCATCCAACATCTCCGCATGATGAAACTTCGGCTC2630.12081955163542817No Hit
GCAGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2500.11484748254318264No Hit
GCTGGACGGGATGCCTGGCTCATAGATGAAATCCCTTAAGCAGGATTGAAGA2410.11071297317162808No Hit
AAGCATGGGAAGATGGTGATGGAACTATAACAACAAAGGAATTGGGAACTGT2300.10565968393972804No Hit
TGTCGTAGGGGCAGTGAATGTGGAAAATTCTATAGCCACAAGTCCAACCTTA2250.1033627342888644No Hit
ACTACCGGGTGCTCCATGCTTTTGCACGTATGATCTGTCAGAATGTGCCTTC2210.10152517456817346No Hit
AGCACGGGGGATGAGAATCAAATCAAGAACTCAGCACCTTTCACAATAGCTG2200.10106578463800074No Hit
CAAAACGGGTACTGCTACGGCCCCCAAGGCAGCAGGAGCCCATACATCCCCC2190.100606394707828No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)