FastQCFastQC Report
Sat 31 Dec 2022
SRR5845654.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845654.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153480
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGCCGTCT8350.5440448266875163No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGC7330.47758665624185564No Hit
GCCCTGGGGTACTCTAATGTTTTATCCTATGATAATCCCACAGTTTGGCCAG1960.1277039353661715No Hit
ACGCTGTGGGGCCCCTGCGCCAAGGGAGCCCACTGCCATCCCCCGTGGGCTG1940.12640083398488403No Hit
TGATAGGGGGACCTGGATGCGAGACATGGAGTGAAAGGGGATCATTTTAAGA1890.12314308053166535No Hit
AAAGCAGGGGGTATTGGTGTAGATAATCTAGTTGGGAATACTTTTAAGTCTC1850.12053687776909043No Hit
TCTTTAGGGGGGGGGTGGGATCTGAACATGGCGGCGGTGGTAGCTGCTACGG1820.11858222569715925No Hit
AAGAGGGGGCCAGCGAAGCTTCCTCCTCCTCTCCAGCTGGTAGTCACTTGCG1720.11206671879072191No Hit
CTAGATGGGTTAATTTTTTATGTAGGTGAGTTATATTTTCTTTCTTTTCTGA1680.10946051602814699No Hit
TGAGGTGGGAGCTGGGGTTCCTTGGACCCCCAAGCCTGGGAAGGGGGAGGTG1670.10880896533750327No Hit
GTGATAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1660.10815741464685953No Hit
TGTTTAGGGAATACTGATAATGACCAACTGCTCATTCTGAAAGCCAAACATA1660.10815741464685953No Hit
CATCTGGGACTTCACAACATGGAACTAACAGACATCTACAGAACAATCCATC1640.10685431326557206No Hit
TAGACGGGGGAGGAGAATGATGGGCGTACAGAGGAGTGAAGAAAGGCTGGGA1630.10620276257492832No Hit
AATGGAGGGGCTCCTGGAGTAAGTTTCACTTGGTCATGGTGTGTCATCCTTT1620.1055512118842846No Hit
ATCTATGGGGATCTCAGGATTAAGAATCTCACTCAAAACCGCTCAACTACAT1620.1055512118842846No Hit
GTAGAAGGGAGCAAACTAAATTTTACTACAATGTATAAAAAGAAGTATACAC1600.10424811050299713No Hit
ACTAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1590.1035965598123534No Hit
CACTTCGGGAGCTCACTGCATCCTCTGCCTCCCGGGTTCAAGCAATTCTCTG1580.10294500912170967No Hit
GTTATCGGGGTGACAAGAGATAAAGAAAGCACAGTATTTTAAAATCTAAAGC1580.10294500912170967No Hit
GAGACTGGGGAGCCCCCTTACCTCTATTACCCAGAAGCTTGTAGGAGGGGTG1580.10294500912170967No Hit
TGTGGAGGGGGAGTTTTGGTTACACGAGTGGCATCAACAGCAGCAGGTGGCA1570.10229345843106594No Hit
GTGCAGGGGGGACCATGTGCAGCGCAGCCTGAGCTGCTGTGGTGTGCAGAAC1560.10164190774042221No Hit
AAACGCGGGGAATGGAAACTTTGTTGAAACTTTAAAAAGCAAATTTACTCCA1560.10164190774042221No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)