FastQCFastQC Report
Sat 31 Dec 2022
SRR5845653.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845653.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences154624
Sequences flagged as poor quality0
Sequence length52
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGCCGTCT8980.5807636589403973No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGC7540.4876345198675497No Hit
ACGCTGTGGGGCCCCTGCGCCAAGGGAGCCCACTGCCATCCCCCGTGGGCTG2670.17267694536423842No Hit
AAAGCAGGGGGTATTGGTGTAGATAATCTAGTTGGGAATACTTTTAAGTCTC2070.13387313741721854No Hit
CACTTCGGGAGCTCACTGCATCCTCTGCCTCCCGGGTTCAAGCAATTCTCTG1870.12093853476821192No Hit
GCCCTGGGGTACTCTAATGTTTTATCCTATGATAATCCCACAGTTTGGCCAG1850.11964507450331126No Hit
TGATAGGGGGACCTGGATGCGAGACATGGAGTGAAAGGGGATCATTTTAAGA1810.11705815397350992No Hit
GGATACGGGTTGTAGATACATGGCATTACTTCTGAGGCCTCTATTGTGTTCC1740.11253104304635761No Hit
GACTATGGGTCCAGTATGTAGCCTGGCGGAACATGATGCCAGAGAACACAGT1740.11253104304635761No Hit
AACTAAGGGAACAGACCAAAAACAAGCAGCGAGATTGAAACGGTAATTAAAA1710.11059085264900663No Hit
GTGCCTGGGGGCCGAGGGTGGGGCGCGGGCGTAGTGGCGCCGGGAGTCGCGG1700.10994412251655629No Hit
GCGCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1700.10994412251655629No Hit
TGTCGGGGCGATCTAACTGCGGGATGACCCCAATACGCAAAACTAACCCCCT1670.10800393211920531No Hit
TGAGGTGGGAGCTGGGGTTCCTTGGACCCCCAAGCCTGGGAAGGGGGAGGTG1670.10800393211920531No Hit
GAGACTGGGGAGCCCCCTTACCTCTATTACCCAGAAGCTTGTAGGAGGGGTG1660.10735720198675497No Hit
AAAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1650.10671047185430464No Hit
TAGTCGGGGCTGCTTAAAGTTGCTCAGGTTGGGTAAAAGCTGTTAAGGCTAA1650.10671047185430464No Hit
CTAGATGGGTTAATTTTTTATGTAGGTGAGTTATATTTTCTTTCTTTTCTGA1650.10671047185430464No Hit
ATCACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1640.1060637417218543No Hit
ATCTATGGGGATCTCAGGATTAAGAATCTCACTCAAAACCGCTCAACTACAT1620.10477028145695363No Hit
TCTGCAGGGTTAGGAAAGAAAAGTTTCTTTTGATGCTACAATCAGTAAAGAG1620.10477028145695363No Hit
TCTTTAGGGGGGGGGTGGGATCTGAACATGGCGGCGGTGGTAGCTGCTACGG1620.10477028145695363No Hit
GTGCAGGGGGGACCATGTGCAGCGCAGCCTGAGCTGCTGTGGTGTGCAGAAC1600.10347682119205298No Hit
AATGGAGGGGCTCCTGGAGTAAGTTTCACTTGGTCATGGTGTGTCATCCTTT1580.10218336092715231No Hit
AGCCCGGGTCCTCCATAAGTGTACAGACGCACTTAGATGTTAAGAGATTAAT1580.10218336092715231No Hit
AGCCTTGGGATTGAATGTTTGCCTTTAGCACCATTTTATTTGGTTTGTCCCA1560.10088990066225165No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)