FastQCFastQC Report
Sat 31 Dec 2022
SRR5845652.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845652.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences153198
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGCCGTCT8470.5528792804083604No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGC7390.4823822765310252No Hit
ACGCTGTGGGGCCCCTGCGCCAAGGGAGCCCACTGCCATCCCCCGTGGGCTG2120.13838300761106542No Hit
CACTTCGGGAGCTCACTGCATCCTCTGCCTCCCGGGTTCAAGCAATTCTCTG2000.1305500071802504No Hit
GGATACGGGTTGTAGATACATGGCATTACTTCTGAGGCCTCTATTGTGTTCC1860.12141150667763285No Hit
ACTAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1800.11749500646222537No Hit
TGATAGGGGGACCTGGATGCGAGACATGGAGTGAAAGGGGATCATTTTAAGA1790.1168422564263241No Hit
AAAGCAGGGGGTATTGGTGTAGATAATCTAGTTGGGAATACTTTTAAGTCTC1780.11618950639042284No Hit
ATCACTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1700.11096750610321285No Hit
GTGCCTGGGGGCCGAGGGTGGGGCGCGGGCGTAGTGGCGCCGGGAGTCGCGG1680.10966200603141033No Hit
GCGCTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1680.10966200603141033No Hit
GTGATAGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1680.10966200603141033No Hit
AAGAGGGGGCCAGCGAAGCTTCCTCCTCCTCTCCAGCTGGTAGTCACTTGCG1660.10835650595960784No Hit
TGTGGAGGGGGAGTTTTGGTTACACGAGTGGCATCAACAGCAGCAGGTGGCA1640.10705100588780532No Hit
GACACCGGGAACCAAAGTTAACAGGGAGAGGATGGGGGAGGGGACAAATTAG1630.10639825585190407No Hit
AATGGAGGGGCTCCTGGAGTAAGTTTCACTTGGTCATGGTGTGTCATCCTTT1610.10509275578010158No Hit
ATCGCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1600.10444000574420031No Hit
GAAACCGGGGAGTATTTATTCCTGTGCTTCAGTCGGTAACAGCCCGAATTAT1600.10444000574420031No Hit
CATCTGGGACTTCACAACATGGAACTAACAGACATCTACAGAACAATCCATC1590.10378725570829907No Hit
TCAAGTGGGACAAGATATGGAGGTGGAAGACAGTGGTATTGATGATCCTGAC1560.10182900560059532No Hit
AGTGATGGGACCTGCCGGGCCGTGACCTCAGCTGGGCGAAGGTTTTCGGTAT1550.10117625556469406No Hit
GAATTTGGGAGCTAGTAACCCATTAAAATGGATAAAAACACTTAGAAGCTCT1540.1005235055287928No Hit
GCCCTGGGGTACTCTAATGTTTTATCCTATGATAATCCCACAGTTTGGCCAG1540.1005235055287928No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)