FastQCFastQC Report
Sat 31 Dec 2022
SRR5845639.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845639.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215677
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGCCGTCT9380.43490961020414787No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGC8580.39781710613556387No Hit
GACTCTGGGGCAGTTTGCTTCTTTTATTAGTAATGTGGATTGATGTTTTCTC2610.12101429452375542No Hit
GCATCAGGGGCAAGTCCCATGAAGCTGAGGTCTTGAAGCAGCTGGCTGAGAA2500.11591407521432512No Hit
TAAGTAGGGGACATGAGAATTGAGAAAAGAAATCAATATTTAGAAGGGTTAC2370.1098865433031802No Hit
AATAATGGGGTAGGCAGTGGCCCCTATTGAGAAGTTTTCCGGTAGAGTTGGA2340.10849557440060832No Hit
GCACAGGGGTTACTAAAAGGAAGACAGGAAGGAAGGAAAGAAGGAAAAGAAG2320.10756826179889373No Hit
TGCAATGGGGAACATCTATTTTGAGAACTCAAAATCCAAGTTTGTGCTTACA2250.10432266769289261No Hit
GCCACCGGGGAGATAGAGGAATATTTACCAAGCAAATGGAAAGCAATAAAAA2250.10432266769289261No Hit
TTCCGTGGGTGAATGCATTCTTTTTTGCTGTTATGGGATATTATGAATTCAC2220.10293169879032071No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)