FastQCFastQC Report
Sat 31 Dec 2022
SRR5845638.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845638.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences216564
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGCCGTCT8970.4141962653072533No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGC8950.41327275078036974No Hit
GACTCTGGGGCAGTTTGCTTCTTTTATTAGTAATGTGGATTGATGTTTTCTC2460.11359228680667148No Hit
TTCCGTGGGTGAATGCATTCTTTTTTGCTGTTATGGGATATTATGAATTCAC2380.10989822869913743No Hit
TTCAGCAGGGGACCTTTTGCCTCGAGGCTGAGCTGGAAAATCTTGAAAATAT2320.10712768511848693No Hit
TAAGTAGGGGACATGAGAATTGAGAAAAGAAATCAATATTTAGAAGGGTTAC2270.10481889880127815No Hit
GCACAGGGGTTACTAAAAGGAAGACAGGAAGGAAGGAAAGAAGGAAAAGAAG2200.10158659795718586No Hit
AACCTGGGGAATTGAAAGAAAGAGATGATATCTTTACATCACTTCCTTGTTT2180.10066308343030235No Hit
GCCACCGGGGAGATAGAGGAATATTTACCAAGCAAATGGAAAGCAATAAAAA2170.1002013261668606No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)