FastQCFastQC Report
Sat 31 Dec 2022
SRR5845618.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845618.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences116300
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGCCGTCT8060.6930352536543423No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGC6720.5778159931212382No Hit
TGGGCTGGGGAGGTCACCATTGCATCAAGGTAATTTCTCATGACAACTACAA3180.27343078245915736No Hit
CTTTGAGGGATTGTAAGCTAAGCATTAGGACTGATACAAATAATATATGCTC2010.17282889079965608No Hit
TAGCTAGGGGGACCCAGGCATGTCATTTTTTCAACACCTCCCTCAAGATGAT1970.16938950988822013No Hit
ATAATTGGGGCTTGCAGCATGGCTGACCAACTGACTGAAGAGCAGATTGCAG1950.16766981943250214No Hit
GTAACTGGGAGGAAAGATCTAAAATTGACACCCTAATGTCACAACTAAAAAG1920.16509028374892518No Hit
GATCTCGGGACCATCCTTCTTTATGAAGGCAAAAACATGAATGGGTATCCAC1920.16509028374892518No Hit
TGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG1890.16251074806534824No Hit
CAGCATGGGTCTAGATTTATTCTACTTACATTCTGGTGATGCTGCCAAGAGA1840.1582115219260533No Hit
AAATGTGGGATCACAAACTGAGTGGCTTAAAACAACAGAAATAGTCTCATAG1840.1582115219260533No Hit
TTAGTTGGGGCTGCACACTTTGCTTTTGTTAAACAGCAGGTAGTAGACAGAA1760.15133276010318142No Hit
GTGACTGGGAAGGTTTGCCTGTTTCGGTTATGAGATTGCAAAAACTAGAAAC1740.14961306964746346No Hit
CTTGAGGGGAAACATACCCTTCCCTCAAATCACCTCAGGTAGCAATCTGTAA1730.14875322441960445No Hit
GGTGCAGGGAAGGCTTCATGGATACATGCTAGTATCCATGTTCCTCTATTCT1700.1461736887360275No Hit
TGCCTTGGGGGGCCTCCGCTGGTATCGGCTGTCGGATGAGGGCGAGTGGCTG1670.14359415305245057No Hit
AAACGTGGGACAGCCAAAGAAATCATACAGAGACTACACTACTAAGCCCATT1670.14359415305245057No Hit
TCTGCAGGACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGC1660.14273430782459157No Hit
GCTGCAGGGGAGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTG1650.1418744625967326No Hit
TCTGAGGGGCCCCAATTCAGCTTAGGTGCCTGAGTTTGTCCTCACTTTGTAT1650.1418744625967326No Hit
CTGACTGGGAGAGTCCAGAGCTGGTGAAGAAGCACAAGAAAAAGAGGGTTGT1620.13929492691315562No Hit
AATTGTGGGGTTGTAGTGGGATGAAATGAGGGAGTAGCTGTAAAGCTTTCAG1620.13929492691315562No Hit
AAGGCCGGGGCCCATTGCACGGGACTTCCTGCACCAGCCGCCGTTTGGGGAG1610.13843508168529664No Hit
AATTTAGGGGACTAGGCTGGAGTTGCTTCCTTGAAGATGGGACTCCTTGGGT1610.13843508168529664No Hit
CATTGCGGGGTCTTTTATAAATTTAGTAAATCAGTTTATAATCTTTAAAGCC1610.13843508168529664No Hit
GATCATGGGAGAGCATATATGACTATTGCTTAATGTTTTTGAGCTTGATTGG1590.13671539122957868No Hit
AAGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1580.13585554600171967No Hit
ATGCCGGGATTTCCAAGAAATCCTGACCATAGTCTGTAGATCTGTTTTTAAA1570.1349957007738607No Hit
GGCCAGGGGTACTAAACCTGAGTAAGGGAAAATGGTAGTGAGAAATGAAGTT1570.1349957007738607No Hit
TAGCTGGGGACCCTTTAGGTTTTAGGAGGCTTGAGAATGGAAGGATCCTCAT1550.13327601031814273No Hit
TAAAGTGGGAGACAGGGATGAGGGTCATTGATCCTGGGTGCAGATCTCCAAA1540.13241616509028373No Hit
CTGGCCGGGATGTTCCCTGGGGAAGCCAATTTGTTCCAACAGCTGCATTACA1540.13241616509028373No Hit
AGAGAGGGAGATGAGGCAATAAACGCTTCATTTCACACTGTGAAATGCACAC1540.13241616509028373No Hit
GCAGTTGGGTCTTTAGATCTGGCTGTCATAATTTATCTGTTTTGCTTCATCC1540.13241616509028373No Hit
GATCCGGGGCAATATACCAAACCTTTGAGATATGGCAAAAGAAGCACTAACA1530.13155631986242475No Hit
GCCTCCGGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATT1520.1306964746345658No Hit
GGTTGCGGACCCTCGCCGCAGCCTGGAGCAGAACATACAGCTACCTGCTGCA1520.1306964746345658No Hit
TCTTGGGGGCGCTACTCAGGAGGAAATTACAGAGACAATTATGACAACTGAA1510.12983662940670682No Hit
GAGTTGGGGATGTCCTCACATGGTAGAAAGACCAGAGGGTAGGAGGGCCTAC1510.12983662940670682No Hit
CAATATGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1500.1289767841788478No Hit
GCTACTGGGCGATCTTGTGCAGGGCAGAGATAATTTGGGGTTTTCTGTTTGA1500.1289767841788478No Hit
AATGGCGGGCAAGGGTGTACGCAAAGCACTCATTGATACCCTTTTGGATGGC1480.12725709372312985No Hit
AACAGTGGGAGCCACATGGTGTGAACCATTCGATTCTCACAACAGAATGGAT1480.12725709372312985No Hit
GCATACGGGCTCCCGAGTCCTGGGCAGGGAAGGGGAGGGAAATTGCTGCTTC1480.12725709372312985No Hit
CGCTATGGGACTTTTATCTTTGTGAATAATTTACTGTCCTTTCCTCCTGGGA1470.12639724849527084No Hit
CCACACGGGGTGATGAAAAGGTTTTGGAACTAGATAGAGGTGGTGGTTGCAC1470.12639724849527084No Hit
GTCAGTGGGGGGCAATCCAGTAGTAGATCTCTTTAGGTATGTGTATTAGTCC1460.12553740326741186No Hit
TGACAAGGGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACAATTAT1460.12553740326741186No Hit
CTTCTTGGGACAAATTAAATATAAAAGTGTGTCTTTCTACAGCCAGTACACT1460.12553740326741186No Hit
AGGCTAGGGATGGACGTCCTCCCCTCCAAATCCAGAAGCTCCCAGAAGGTGT1450.12467755803955288No Hit
TAGTAGAGGGGGGAATTCAGCTAGGACTAGGGGTGAGTGGTTAGTTAATGGA1440.1238177128116939No Hit
TTACGAGGGGTAGGTTCAAAAGGTTTCGAGGTGGCACCTAACCAGAATGTGG1440.1238177128116939No Hit
ATTAGCGGGGGGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1440.1238177128116939No Hit
CGCTAAGGGGTTCCAACAATCAGCGAAGTAGGAAATGTTTCGGAGACACTAA1440.1238177128116939No Hit
TGACGCGGAGATGAGCCTCAGGCATCCCAAGAGGAATGCACTTTCCCAGAGA1430.12295786758383491No Hit
CCTGATGGGTGTCTTCTTTTCTCTTGTATAATCAAAGAAATGCAAGCATGAG1430.12295786758383491No Hit
ACAAGCGGGGGGCCTGGCTACATGTTCTTCATTCCTGTTTAATAAATCTCAT1420.12209802235597592No Hit
CGAATTGGGTAAAATCCTGGACAGAAACTGAAATATATATCTGAAAGATATG1420.12209802235597592No Hit
GGCTATGGGAACGATCTTGCATTCTGGATTCTTGCAGCAAAGTCTCAGATAC1420.12209802235597592No Hit
CGTATAGGGATTCTCATGAAATGTGCTTGGTCTGTGATCTCTTGGTCAGATA1410.12123817712811694No Hit
TTTCCAGGGCGATCTGAATAGGGGCAATTAACAGCCCAATATCTACAATCAA1410.12123817712811694No Hit
ATTGCAGGGATACTGGATCAAAGAAGAATTAATAGGGAAATTAGAAAATAAT1390.11951848667239896No Hit
TGTTCAGGGGATCGATGATGAGAATAATTGTCTGAGGATGCTGAGGGACTCA1390.11951848667239896No Hit
TATCATGGGAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTG1390.11951848667239896No Hit
TAGCTGGGGATGCCTTTCCTAAGAACTCAAAGTTCTCTTTTCAGCGTGCTGT1390.11951848667239896No Hit
AGTAGTGGGAAGTGTTTCGTATCAGGATGGCCTCAGACCACGGGAAGGAACT1380.11865864144453997No Hit
TATAACGGGCTGTCACTTGCAGTACCATAGCTAAGTCTGCTATCTCCAGCTA1360.11693895098882201No Hit
GACGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1350.11607910576096302No Hit
CAAGGAGGGAAGACAGAAAGACAAACCAAAAGAAAACAAAAGTAGAGAGAAA1350.11607910576096302No Hit
TGCTGCGGGCATATTCCCTGCCCCTCCAGATACTTACAAAAAATGATGACAT1350.11607910576096302No Hit
AGGAGCGGGACTGCACAACTGCATGGAAACTGAACAACCTGCTCCTGAATGA1340.11521926053310405No Hit
TTTACAGGGTACTCAAAGACATAGATGTGAAAATTTTAGGCAACCTTCTAAA1340.11521926053310405No Hit
CTAACGCAGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG1340.11521926053310405No Hit
CCAACCGGGTCCGATCTCAGTCAGGGATGGCGATTCCACACCAAGTACATGA1340.11521926053310405No Hit
CGTTGCGGGGAACATAAGGCAAATTATGGAAATCATTATCAGCTGACTCAGC1330.11435941530524507No Hit
TACTCAGGGGATCTATGATAGGGAACCAGTAACTAATATTTCAGCTGTTGAA1330.11435941530524507No Hit
TTGATGGGAATAAAACTTATTATTGAGCAAAAGTTCAATTAAAAATGCTCAA1330.11435941530524507No Hit
ACGTTGGGACAGTGAAAGAGTAAATTAGAAAGTGAATGGATGACTGTTCATG1320.11349957007738608No Hit
AAATCAGGGGGGCAAATGGATAACTTAACCAGTCCATATATGGTTTTAAAGT1320.11349957007738608No Hit
TACGATAGGGGATTTTAAGTACTGACCAGTGTTCAATTTTTATTTTAGATTT1320.11349957007738608No Hit
CACTCCGGGACTTGCTATTTTAGAAATTATGGATCCGGATGAGCAATTGGAA1320.11349957007738608No Hit
GCTTCAGGGTGCATTCATCATGGCTGCCGGCCCAGCCTCCTTCTGCTGCCAC1320.11349957007738608No Hit
TACACAAGGGAAGAGGAAGACAAAACAAAACAAAAACCTCAAAACCAAAAAA1310.1126397248495271No Hit
GTTCTGAGGCAAAATCTTTCCAAATCAATTATATTTTGCTGCTTTTCTTTAA1310.1126397248495271No Hit
TCTACGGGGATTGCCTGTGATCTTTACGCTTAACTGTTGTGTATCTTTTTTG1310.1126397248495271No Hit
TCAGGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1300.1117798796216681No Hit
AGTAGGGGACTCACAAGGGATCCTTAGGACATCTGGATCTGACCTACAGCCT1300.1117798796216681No Hit
AAGTGGGGAAGTTGTACAAAATTATTAGTTAGCAGTAACCAGTTTTCAAGGA1290.11092003439380912No Hit
CTTAGGGCTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGC1290.11092003439380912No Hit
CTCAACGGGCCATGAGTCTTTCTCCTATATTGTGAGCATTGCGTATTCCAAA1280.11006018916595013No Hit
AGGACAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1280.11006018916595013No Hit
GAATCCGGGCGCCCAGCCGGAGGGCCCCGGGGGTGCTGCGAGCTGGGAATAT1280.11006018916595013No Hit
TAGCAAGGGTACTCGAGGGTAGTGATGCCCTTTTTCCATATTCCTTTTTTTT1270.10920034393809115No Hit
TAATGAGGGCATCCCAAAAGTTATATACACAATTGCCTTTAAAACCACAAGA1270.10920034393809115No Hit
CTAGCCGGGATTCTCATCATAGATACGTGCAGTATCTTTAATGAGTATCTTC1270.10920034393809115No Hit
TAGCATGGGCAGTTCCAACAAACATCATGATAGTCTGGTAGTCAAGACAGAG1260.10834049871023216No Hit
ATAACTGGGTAATTTTTACTTATTTAATGAGCTTTTCCTCTCATGGCATATT1260.10834049871023216No Hit
TGTAACGGGGGTCCGCATCGTGGTGGAGTACTGTGAACCCTGCGGCTTCGAG1260.10834049871023216No Hit
GTCAATGGGATGAATGAGAAATTAGTTAACATTCTGCTCCACAAGGTAAGAA1260.10834049871023216No Hit
GGTGCTGGGAGAAATGTTCATTTTTGTTTTAAAATTTGTTTTCCATGTGAAG1260.10834049871023216No Hit
TAGCGTGGGGAGACTTTTCATTTTGTGGGAGAGTAGCAGCAAGTCCTTGGAA1260.10834049871023216No Hit
TATTGAGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1250.10748065348237318No Hit
ATTAGAGGGGGTGTTTTAAGCTTGAGTGAAAGGAGGGTGAGGCTGCCGATGC1250.10748065348237318No Hit
AACAACGGGAGAGGGGATGTGGTGAGAGCCAGGTTCCTCTGGCCCTCTCCAG1250.10748065348237318No Hit
ATCTAGGGGACGCTCTTCCGATCTGGTAACGGGGGAAGTGGCCCTTGCAGGC1240.10662080825451418No Hit
ACTGTGGGGTGATGCATTTGACCACATTACAATTAAGAATTTCTATTCAGCA1240.10662080825451418No Hit
TAACGCGGGGAAGAGGGCACCTGAGGAGACTTTGGGGACAGGGCAGGGGCAG1240.10662080825451418No Hit
TGACCAGGGCGATCTCAAGACGGGAGAAGAGATAAGACATATACACAAATAG1240.10662080825451418No Hit
AATGCTGGGATATCCTGACATTTTATGTTTCTGACATTTGTTTCACTGATTT1240.10662080825451418No Hit
CTAACCGGGCTGGGCAAGCTACCTCGAACCCTGTCGTTAATTACTTCTGGTA1230.1057609630266552No Hit
AGACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1230.1057609630266552No Hit
TGTGCAGGGGTCTTCTGGAGAAATGAATTCAGAAGAAAATTCATCAGGTGCT1230.1057609630266552No Hit
TGGTGCGGGCACTTGGACATGTGTCCCTGTTACCTGAGAATGACAGATGGAA1230.1057609630266552No Hit
TTGGGCGCTCTTCCGATCATGGCCAGGATTGAGTCCTATGAAGGAAGGGAAA1220.10490111779879621No Hit
AGAACAGGGACTTTGAAAACGACTGCTGGGTCAGGTACTTCCTGCACACAGG1220.10490111779879621No Hit
TGACCGGGGCTGAGACAACTAAAAGTTAAAATGTAGGGGAATGGAGTTAAAA1210.10404127257093723No Hit
TGATAAGGGGGGCCACAGTAATTACAAACTTACTATCCGCCATCCCATACAT1210.10404127257093723No Hit
AGCAAAGGGCGATCTTCGAACGGGACAATTCAATGTATTTTCACAAACTGGT1210.10404127257093723No Hit
CAAACAGGGAGACTAACTCAGAGACAGCTGTGGTGGCCCTCACTCAGGACCA1200.10318142734307824No Hit
CATCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1200.10318142734307824No Hit
TCTAAAGGGAATCGCCCCATTCTCTTTTACGGTTCAAAATGTACTGCTGACC1200.10318142734307824No Hit
TGTGGTGGGCTGGGGTATATCCTGTCTGTGTTCCCAGCCACACTGCAATGAC1190.10232158211521926No Hit
GGAAATGGGCTGGAAAAGGCAAAAGACTTTAGAGGATATCCACTCACTTTAG1190.10232158211521926No Hit
GCACACGGGCGGGGACTCTCAGCGGCTTGACCAGAGTGAGGCGCTTCCAGCA1190.10232158211521926No Hit
CTTGGCTGGGCGATCTGCCGACGGGATTGTAGTTGTTCAGAAATATGCTTTT1190.10232158211521926No Hit
CAGCTGGGGAACTGATAAGAGTGGAGGAGGAATGCCTTCTCCTAAGAGTCAG1180.10146173688736027No Hit
TAGTATGGGTTCATTTGGCTTCGCATTATGCAAGGTGAAACCGTTTGTTTTC1180.10146173688736027No Hit
GAGCATGGGCGAGTAGAATAACTTTTGCTCTATGTGAGAAGATACGTTTTAA1180.10146173688736027No Hit
CTCGCAGGGACCAAACTTTGCTTAAAGCAGCATAGTTACATTGAGAAAGAGA1180.10146173688736027No Hit
TGTCATGGGAGAACTTGTTCTCGTTCTAAAAGCTTCCCAACTAACAAATCAT1180.10146173688736027No Hit
GTCAGTGGGCAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGC1180.10146173688736027No Hit
TTATTTGGGGTCCTTTCATGTAATGTCTGATTTTTATGTGGAAGAGAATGTA1180.10146173688736027No Hit
TTGTTTGGGTTGGAGTCGGGATTCCCCTTTCCCAAACATGCGTCTCGCCACT1180.10146173688736027No Hit
ATAGCAGGGATAGACATCAGAGGGTTCACACTGGAGAGAAACCTTATAAATG1170.10060189165950129No Hit
AATCGTGGGAGGCCAACAGGCCAGCCCTTGAATGGGGATTTATTACTGTTGT1170.10060189165950129No Hit
AGTAGCGGGAACACATAGCCTACCCCTTCCTTGTACTATCCCTATGAGGCAT1170.10060189165950129No Hit
GCTAGAGGGCACGGCGGGACCGGATTCGCCGGAATTGACTCCAGCTCTCCAG1170.10060189165950129No Hit
CGGCTGGGGGTCAGTGCTGACAAATAACATTTAACAAGTATTGCAGTAATCA1170.10060189165950129No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)