FastQCFastQC Report
Sat 31 Dec 2022
SRR5845617.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845617.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences117384
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGCCGTCT8480.722415320656989No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGC6530.5562938731002521No Hit
TGGGCTGGGGAGGTCACCATTGCATCAAGGTAATTTCTCATGACAACTACAA3120.26579431609077897No Hit
CTTTGAGGGATTGTAAGCTAAGCATTAGGACTGATACAAATAATATATGCTC2330.19849383220881894No Hit
CAGCATGGGTCTAGATTTATTCTACTTACATTCTGGTGATGCTGCCAAGAGA2130.18145573502351256No Hit
TAGCTAGGGGGACCCAGGCATGTCATTTTTTCAACACCTCCCTCAAGATGAT2070.17634430586792066No Hit
ATAATTGGGGCTTGCAGCATGGCTGACCAACTGACTGAAGAGCAGATTGCAG2050.17464049614939003No Hit
GTGACTGGGAAGGTTTGCCTGTTTCGGTTATGAGATTGCAAAAACTAGAAAC1980.16867716213453282No Hit
CTGGCCGGGATGTTCCCTGGGGAAGCCAATTTGTTCCAACAGCTGCATTACA1960.1669733524160022No Hit
GATCTCGGGACCATCCTTCTTTATGAAGGCAAAAACATGAATGGGTATCCAC1900.1618619232604103No Hit
CTTGAGGGGAAACATACCCTTCCCTCAAATCACCTCAGGTAGCAATCTGTAA1900.1618619232604103No Hit
AAACGTGGGACAGCCAAAGAAATCATACAGAGACTACACTACTAAGCCCATT1890.16101001840114496No Hit
AATTGTGGGGTTGTAGTGGGATGAAATGAGGGAGTAGCTGTAAAGCTTTCAG1810.1541947795270224No Hit
GCCTCCGGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATT1780.15163906494922647No Hit
AAGGCCGGGGCCCATTGCACGGGACTTCCTGCACCAGCCGCCGTTTGGGGAG1740.1482314455121652No Hit
GATCCGGGGCAATATACCAAACCTTTGAGATATGGCAAAAGAAGCACTAACA1740.1482314455121652No Hit
AATTTAGGGGACTAGGCTGGAGTTGCTTCCTTGAAGATGGGACTCCTTGGGT1730.14737954065289988No Hit
ATTAGCGGGGGGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1730.14737954065289988No Hit
ATGCCGGGATTTCCAAGAAATCCTGACCATAGTCTGTAGATCTGTTTTTAAA1710.14567573093436925No Hit
GTAACTGGGAGGAAAGATCTAAAATTGACACCCTAATGTCACAACTAAAAAG1710.14567573093436925No Hit
GCATACGGGCTCCCGAGTCCTGGGCAGGGAAGGGGAGGGAAATTGCTGCTTC1670.14226811149730798No Hit
AATGCTGGGATATCCTGACATTTTATGTTTCTGACATTTGTTTCACTGATTT1670.14226811149730798No Hit
TTAGTTGGGGCTGCACACTTTGCTTTTGTTAAACAGCAGGTAGTAGACAGAA1640.13971239691951204No Hit
TCTGAGGGGCCCCAATTCAGCTTAGGTGCCTGAGTTTGTCCTCACTTTGTAT1630.13886049206024673No Hit
CTGACTGGGAGAGTCCAGAGCTGGTGAAGAAGCACAAGAAAAAGAGGGTTGT1600.13630477748245076No Hit
GTCAATGGGATGAATGAGAAATTAGTTAACATTCTGCTCCACAAGGTAAGAA1600.13630477748245076No Hit
GGTGCAGGGAAGGCTTCATGGATACATGCTAGTATCCATGTTCCTCTATTCT1570.13374906290465483No Hit
TGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG1560.13289715804538949No Hit
CAATATGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1560.13289715804538949No Hit
GCTGCAGGGGAGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTG1550.13204525318612417No Hit
TACTCAGGGGATCTATGATAGGGAACCAGTAACTAATATTTCAGCTGTTGAA1550.13204525318612417No Hit
TCTGCAGGACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGC1540.13119334832685886No Hit
GATCATGGGAGAGCATATATGACTATTGCTTAATGTTTTTGAGCTTGATTGG1540.13119334832685886No Hit
GGCCAGGGGTACTAAACCTGAGTAAGGGAAAATGGTAGTGAGAAATGAAGTT1530.13034144346759355No Hit
CCTGATGGGTGTCTTCTTTTCTCTTGTATAATCAAAGAAATGCAAGCATGAG1530.13034144346759355No Hit
CAAGGAGGGAAGACAGAAAGACAAACCAAAAGAAAACAAAAGTAGAGAGAAA1510.1286376337490629No Hit
CAAACAGGGAGACTAACTCAGAGACAGCTGTGGTGGCCCTCACTCAGGACCA1500.12778572888979758No Hit
CATTGCGGGGTCTTTTATAAATTTAGTAAATCAGTTTATAATCTTTAAAGCC1500.12778572888979758No Hit
AAGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1490.12693382403053227No Hit
TATCATGGGAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTG1490.12693382403053227No Hit
CCAACCGGGTCCGATCTCAGTCAGGGATGGCGATTCCACACCAAGTACATGA1490.12693382403053227No Hit
AAGTGGGGAAGTTGTACAAAATTATTAGTTAGCAGTAACCAGTTTTCAAGGA1490.12693382403053227No Hit
TAGATGGGAGTGAAAGAGTAAATTAGAAAGTGAATGGATGACTGTTCATGAG1480.12608191917126693No Hit
TGCCTTGGGGGGCCTCCGCTGGTATCGGCTGTCGGATGAGGGCGAGTGGCTG1480.12608191917126693No Hit
TGACCAGGGCGATCTCAAGACGGGAGAAGAGATAAGACATATACACAAATAG1470.12523001431200162No Hit
CCACACGGGGTGATGAAAAGGTTTTGGAACTAGATAGAGGTGGTGGTTGCAC1470.12523001431200162No Hit
TACACAAGGGAAGAGGAAGACAAAACAAAACAAAAACCTCAAAACCAAAAAA1460.12437810945273632No Hit
TCTTGGGGGCGCTACTCAGGAGGAAATTACAGAGACAATTATGACAACTGAA1450.123526204593471No Hit
TATTGAGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1450.123526204593471No Hit
ATAACTGGGTAATTTTTACTTATTTAATGAGCTTTTCCTCTCATGGCATATT1440.12267429973420568No Hit
AACTGTGGGACTCAATCACCTAGCTAAAAATGGTTCAAATGGCAGTTTGTCA1440.12267429973420568No Hit
CTTGGCTGGGCGATCTGCCGACGGGATTGTAGTTGTTCAGAAATATGCTTTT1440.12267429973420568No Hit
AGTAGTGGGAAGTGTTTCGTATCAGGATGGCCTCAGACCACGGGAAGGAACT1430.12182239487494037No Hit
GCTTCAGGGTGCATTCATCATGGCTGCCGGCCCAGCCTCCTTCTGCTGCCAC1430.12182239487494037No Hit
TGACCGGGGCTGAGACAACTAAAAGTTAAAATGTAGGGGAATGGAGTTAAAA1420.12097049001567506No Hit
TAGCTGGGGATGCCTTTCCTAAGAACTCAAAGTTCTCTTTTCAGCGTGCTGT1420.12097049001567506No Hit
GGCTATGGGAACGATCTTGCATTCTGGATTCTTGCAGCAAAGTCTCAGATAC1420.12097049001567506No Hit
GCTACTGGGCGATCTTGTGCAGGGCAGAGATAATTTGGGGTTTTCTGTTTGA1420.12097049001567506No Hit
ACTGTGGGGTGATGCATTTGACCACATTACAATTAAGAATTTCTATTCAGCA1410.12011858515640972No Hit
TGACGCGGAGATGAGCCTCAGGCATCCCAAGAGGAATGCACTTTCCCAGAGA1410.12011858515640972No Hit
TGACAAGGGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACAATTAT1390.1184147754378791No Hit
AAATGTGGGATCACAAACTGAGTGGCTTAAAACAACAGAAATAGTCTCATAG1390.1184147754378791No Hit
GACATTGGGACTGTTAAGAGTCCTTTTGAATTAAACTCGACTAAAACAAGTG1380.11756287057861378No Hit
TGTAACGGGGGTCCGCATCGTGGTGGAGTACTGTGAACCCTGCGGCTTCGAG1380.11756287057861378No Hit
GATCCCGGGGTAGGAGTTTGGGCAAGTTAAAAAATCAGAGCTAAGTCCTCAC1380.11756287057861378No Hit
AGGACAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1370.11671096571934847No Hit
TTACGAGGGGTAGGTTCAAAAGGTTTCGAGGTGGCACCTAACCAGAATGTGG1370.11671096571934847No Hit
GCAAGCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1370.11671096571934847No Hit
AAATCAGGGGGGCAAATGGATAACTTAACCAGTCCATATATGGTTTTAAAGT1360.11585906086008316No Hit
TTTCCAGGGCGATCTGAATAGGGGCAATTAACAGCCCAATATCTACAATCAA1360.11585906086008316No Hit
GTCAGTGGGCAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGC1360.11585906086008316No Hit
TAAAGTGGGAGACAGGGATGAGGGTCATTGATCCTGGGTGCAGATCTCCAAA1350.11500715600081782No Hit
CAATAAGGGCAATTGAAAGAAGAAGAAAATAAACGGTTAAATCAAAGACTGA1350.11500715600081782No Hit
AGTAGGGGACTCACAAGGGATCCTTAGGACATCTGGATCTGACCTACAGCCT1340.1141552511415525No Hit
CGCTATGGGACTTTTATCTTTGTGAATAATTTACTGTCCTTTCCTCCTGGGA1330.11330334628228719No Hit
GTTCTGAGGCAAAATCTTTCCAAATCAATTATATTTTGCTGCTTTTCTTTAA1330.11330334628228719No Hit
GGTTGCGGACCCTCGCCGCAGCCTGGAGCAGAACATACAGCTACCTGCTGCA1330.11330334628228719No Hit
AATGGCGGGCAAGGGTGTACGCAAAGCACTCATTGATACCCTTTTGGATGGC1330.11330334628228719No Hit
TAACGCGGGGAAGAGGGCACCTGAGGAGACTTTGGGGACAGGGCAGGGGCAG1330.11330334628228719No Hit
GTGAAGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1320.11245144142302188No Hit
AGACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1320.11245144142302188No Hit
GGCACAGGGATGCCCAGAATAGAAAAGCTATGTTGGGGCACATGTCCTGCAA1320.11245144142302188No Hit
CTTCTTGGGACAAATTAAATATAAAAGTGTGTCTTTCTACAGCCAGTACACT1320.11245144142302188No Hit
TAGTACGGGGTGTTGCAGCCTGCTGCGCGCCCAGGGGTCCCGCGGGTTTTCG1320.11245144142302188No Hit
TGCTGCGGGCATATTCCCTGCCCCTCCAGATACTTACAAAAAATGATGACAT1320.11245144142302188No Hit
GTCAGTGGGGGGCAATCCAGTAGTAGATCTCTTTAGGTATGTGTATTAGTCC1310.11159953656375657No Hit
TTGATGGGAATAAAACTTATTATTGAGCAAAAGTTCAATTAAAAATGCTCAA1310.11159953656375657No Hit
GCCGCAGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATTG1310.11159953656375657No Hit
TCAGGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1300.11074763170449126No Hit
GGTGGTGGGATATGATTCACTCTCTTTGTTCCAGAGCAGATACAAATTATCA1300.11074763170449126No Hit
AGAGAGGGAGATGAGGCAATAAACGCTTCATTTCACACTGTGAAATGCACAC1300.11074763170449126No Hit
TAGCGTGGGGAGACTTTTCATTTTGTGGGAGAGTAGCAGCAAGTCCTTGGAA1300.11074763170449126No Hit
AGGTTTGGGACAGCATAAATCTGTTAGCATTATTGCCAAATAAGACAGTTGG1290.10989572684522593No Hit
TAGCTGGGGACCCTTTAGGTTTTAGGAGGCTTGAGAATGGAAGGATCCTCAT1290.10989572684522593No Hit
AGGTTCGGGAATGTTGCAAACTACCAGAAGCAAATGAACTCATGTCCAGTAC1290.10989572684522593No Hit
TACGATAGGGGATTTTAAGTACTGACCAGTGTTCAATTTTTATTTTAGATTT1290.10989572684522593No Hit
TTGTTTGGGTTGGAGTCGGGATTCCCCTTTCCCAAACATGCGTCTCGCCACT1290.10989572684522593No Hit
AGGAGCGGGACTGCACAACTGCATGGAAACTGAACAACCTGCTCCTGAATGA1280.1090438219859606No Hit
ATTGCAGGGATACTGGATCAAAGAAGAATTAATAGGGAAATTAGAAAATAAT1280.1090438219859606No Hit
CCCATAGGGAAGTACCTAGGGGAGAAAGAGCCATGTAAATATCTGTAATAAA1280.1090438219859606No Hit
ACTTGCGGGTAGCCGGGAAATGATAAAAGGTTTTGTAGCTACTTTGGTTCAT1280.1090438219859606No Hit
ATTAGAGGGGGTGTTTTAAGCTTGAGTGAAAGGAGGGTGAGGCTGCCGATGC1280.1090438219859606No Hit
AGACAAGGGATTGTTTGATTTCTCTTTGTCAAGTGTATAGAACCTGTCATAC1280.1090438219859606No Hit
CGTATAGGGATTCTCATGAAATGTGCTTGGTCTGTGATCTCTTGGTCAGATA1270.10819191712669529No Hit
AACTAGGGATTTGCCTTCTGCCTCTACATTAGAGAACAGTAATTGCTTCCCT1270.10819191712669529No Hit
CAGAGAAGGGAAGGCTGGAGATGGTCCCAGATCCAAGATGTCCCAAAAGCCT1260.10734001226742997No Hit
CTAACGCAGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAG1260.10734001226742997No Hit
ATAAGCGGCATCTGCAAACAGAGAAAATTTTACTTCCTCTCTTCCTGTTTGA1260.10734001226742997No Hit
GCAGTTGGGTCTTTAGATCTGGCTGTCATAATTTATCTGTTTTGCTTCATCC1260.10734001226742997No Hit
AGCTCAGGGTACAAAAATTAGCAGGGCGTGGTGGTGCGCCTATATTCTCAGC1260.10734001226742997No Hit
TGATAAGGGGGGCCACAGTAATTACAAACTTACTATCCGCCATCCCATACAT1250.10648810740816465No Hit
CTATATGGGACTTTTTATTGTGCATTTTTATTGACACTTTAATGACTCCCCC1250.10648810740816465No Hit
GTGACAGGGATACATGGAATAGTTATATTTTAATTAAGCATTTATTTTAGTT1240.10563620254889934No Hit
CTCAACGGGCCATGAGTCTTTCTCCTATATTGTGAGCATTGCGTATTCCAAA1240.10563620254889934No Hit
TAGCATGGGCAGTTCCAACAAACATCATGATAGTCTGGTAGTCAAGACAGAG1240.10563620254889934No Hit
TGTAGAGGGCAAGCTGGGTTCTTTGTGGAAGAAGGACAGGGAGCTAGAGCCA1240.10563620254889934No Hit
TAGCAAGGGTACTCGAGGGTAGTGATGCCCTTTTTCCATATTCCTTTTTTTT1230.10478429768963403No Hit
TGTCATGGGAGAACTTGTTCTCGTTCTAAAAGCTTCCCAACTAACAAATCAT1230.10478429768963403No Hit
CACTCCGGGACTTGCTATTTTAGAAATTATGGATCCGGATGAGCAATTGGAA1230.10478429768963403No Hit
TCTAAAGGGAATCGCCCCATTCTCTTTTACGGTTCAAAATGTACTGCTGACC1230.10478429768963403No Hit
ATCTAGGGGACGCTCTTCCGATCTGGTAACGGGGGAAGTGGCCCTTGCAGGC1220.10393239283036869No Hit
TAGTTGCGGGGGGAGTCGAGCCAGCGTCGCCGCGATGGTGTTGTTGGAGAGC1220.10393239283036869No Hit
TTGGGCGCTCTTCCGATCATGGCCAGGATTGAGTCCTATGAAGGAAGGGAAA1220.10393239283036869No Hit
ACGTTGGGACAGTGAAAGAGTAAATTAGAAAGTGAATGGATGACTGTTCATG1220.10393239283036869No Hit
CGCTTAGGGAGGTTCCTCTGGAGCTAAAGCGTAGTGTGGGGCTCAGTAAACT1220.10393239283036869No Hit
GAATCCGGGCGCCCAGCCGGAGGGCCCCGGGGGTGCTGCGAGCTGGGAATAT1220.10393239283036869No Hit
AGGCTAGGGATGGACGTCCTCCCCTCCAAATCCAGAAGCTCCCAGAAGGTGT1220.10393239283036869No Hit
TCAGCAGGGACATGTAAGTAGAATAATACCACAGATGATTCTGCCTGGAACC1220.10393239283036869No Hit
CGCTAAGGGGTTCCAACAATCAGCGAAGTAGGAAATGTTTCGGAGACACTAA1220.10393239283036869No Hit
TATAACGGGCTGTCACTTGCAGTACCATAGCTAAGTCTGCTATCTCCAGCTA1220.10393239283036869No Hit
GGGTACGGGACAATGGTTTTGTTTTTTTAAAACAAGATATCACAGTATACAA1210.10308048797110338No Hit
TTCTAAGGGGAAATTTATAGATGGGACAATGCACATACTTCATATGTAAACA1210.10308048797110338No Hit
TGTGGTGGGCTGGGGTATATCCTGTCTGTGTTCCCAGCCACACTGCAATGAC1210.10308048797110338No Hit
AAAATCGGGCTCTGTTCAGAAGTTCTGTCTATATGTGGGAAGAATGAAAGTT1210.10308048797110338No Hit
CGAATTGGGTAAAATCCTGGACAGAAACTGAAATATATATCTGAAAGATATG1210.10308048797110338No Hit
TTGACAGGGACACACTCCTTGCTGCGGGCTCCTGCAGCCAGACCTGAGCAGA1210.10308048797110338No Hit
TTGTGCCGGGAGGAGGCATAGTGATTCTTAAATATTTACGGAACATTTGGTG1210.10308048797110338No Hit
AACAACGGGAGAGGGGATGTGGTGAGAGCCAGGTTCCTCTGGCCCTCTCCAG1210.10308048797110338No Hit
CTACACGTGGGGAGCGGGGAGGCGGCGGCTCCAGGGACCTGGCGGCCGCCGA1210.10308048797110338No Hit
AACAGTGGGAGCCACATGGTGTGAACCATTCGATTCTCACAACAGAATGGAT1210.10308048797110338No Hit
CAGCTGGGGAACTGATAAGAGTGGAGGAGGAATGCCTTCTCCTAAGAGTCAG1200.10222858311183806No Hit
CACAAGGGGGATCTTGTCGGGGGACAGACAGATGTCTTTTATGAATGAATAT1200.10222858311183806No Hit
GCACACGGGCGGCAACAAGAGCGTCCTGATGGAGCGGCTCAAGAAGGCGGTT1200.10222858311183806No Hit
GCTAGAGGGCACGGCGGGACCGGATTCGCCGGAATTGACTCCAGCTCTCCAG1200.10222858311183806No Hit
CTCTGCATGGGTTTGGTAACATCTCTACTATGGAAACAAATTAAAAACCAGA1190.10137667825257275No Hit
TATAGTAGGGAGGCAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1190.10137667825257275No Hit
CGTATGGGATGAGGGTACTTTTTTGGTTTTGCTGGCTTAATATTGTGTATTG1190.10137667825257275No Hit
CGTTGCGGGGAACATAAGGCAAATTATGGAAATCATTATCAGCTGACTCAGC1180.10052477339330744No Hit
TCGACAGGGACCCAGGCTGGAGTGCAGTGGCGCGATCTCAGCTCACTGCAAC1180.10052477339330744No Hit
GATAATGGGATAGTCAAAATGTGGAGATTATTGATATATGAAACAGAAGCCA1180.10052477339330744No Hit
GTAAATGGGCGCAGGACAGCTGCCCACAAAAAGGAGTCCAGCCTAGGAATCC1180.10052477339330744No Hit
CACATGGGAGAGTTGTCATTTGCTTCACCCCAATTGTCAATATCTCAGTTAA1180.10052477339330744No Hit
TTATTTGGGGTCCTTTCATGTAATGTCTGATTTTTATGTGGAAGAGAATGTA1180.10052477339330744No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)