FastQCFastQC Report
Sat 31 Dec 2022
SRR5845616.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845616.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114501
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGCCGTCT8230.7187710151003047No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGATTACCATCTCGTATGC6370.5563270189780002No Hit
TGGGCTGGGGAGGTCACCATTGCATCAAGGTAATTTCTCATGACAACTACAA2500.21833870446546316No Hit
TAGCTAGGGGGACCCAGGCATGTCATTTTTTCAACACCTCCCTCAAGATGAT2170.18951799547602205No Hit
CTTTGAGGGATTGTAAGCTAAGCATTAGGACTGATACAAATAATATATGCTC2110.18427786656885092No Hit
GATCTCGGGACCATCCTTCTTTATGAAGGCAAAAACATGAATGGGTATCCAC1930.16855747984733757No Hit
AATTTAGGGGACTAGGCTGGAGTTGCTTCCTTGAAGATGGGACTCCTTGGGT1920.16768412502947572No Hit
AAGCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAG1870.16331735094016647No Hit
GTAACTGGGAGGAAAGATCTAAAATTGACACCCTAATGTCACAACTAAAAAG1860.16244399612230462No Hit
GTGACTGGGAAGGTTTGCCTGTTTCGGTTATGAGATTGCAAAAACTAGAAAC1800.1572038672151335No Hit
ATAATTGGGGCTTGCAGCATGGCTGACCAACTGACTGAAGAGCAGATTGCAG1790.15633051239727164No Hit
TATCATGGGAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTG1780.15545715757940978No Hit
CAGCATGGGTCTAGATTTATTCTACTTACATTCTGGTGATGCTGCCAAGAGA1740.15196373830796236No Hit
AAACGTGGGACAGCCAAAGAAATCATACAGAGACTACACTACTAAGCCCATT1740.15196373830796236No Hit
ATGCCGGGATTTCCAAGAAATCCTGACCATAGTCTGTAGATCTGTTTTTAAA1680.14672360940079127No Hit
AAGGCCGGGGCCCATTGCACGGGACTTCCTGCACCAGCCGCCGTTTGGGGAG1670.1458502545829294No Hit
GATCATGGGAGAGCATATATGACTATTGCTTAATGTTTTTGAGCTTGATTGG1630.142356835311482No Hit
CAATATGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1630.142356835311482No Hit
TGCCTTGGGGGGCCTCCGCTGGTATCGGCTGTCGGATGAGGGCGAGTGGCTG1620.14148348049362014No Hit
CTGGCCGGGATGTTCCCTGGGGAAGCCAATTTGTTCCAACAGCTGCATTACA1620.14148348049362014No Hit
CTTGAGGGGAAACATACCCTTCCCTCAAATCACCTCAGGTAGCAATCTGTAA1600.13973677085789643No Hit
AAATGTGGGATCACAAACTGAGTGGCTTAAAACAACAGAAATAGTCTCATAG1590.1388634160400346No Hit
GATCCGGGGCAATATACCAAACCTTTGAGATATGGCAAAAGAAGCACTAACA1580.13799006122217275No Hit
TGCTGCGGGCATATTCCCTGCCCCTCCAGATACTTACAAAAAATGATGACAT1580.13799006122217275No Hit
GCTGCAGGGGAGGGGCGGTTGGCTTTGTTGGGTGAGCTTGTTTGTGTCCCTG1570.1371167064043109No Hit
ATTAGCGGGGGGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1570.1371167064043109No Hit
CTGACTGGGAGAGTCCAGAGCTGGTGAAGAAGCACAAGAAAAAGAGGGTTGT1560.13624335158644904No Hit
GGCCAGGGGTACTAAACCTGAGTAAGGGAAAATGGTAGTGAGAAATGAAGTT1560.13624335158644904No Hit
TGACGCGGAGATGAGCCTCAGGCATCCCAAGAGGAATGCACTTTCCCAGAGA1560.13624335158644904No Hit
GTCAATGGGATGAATGAGAAATTAGTTAACATTCTGCTCCACAAGGTAAGAA1520.1327499323150016No Hit
GCTACTGGGCGATCTTGTGCAGGGCAGAGATAATTTGGGGTTTTCTGTTTGA1520.1327499323150016No Hit
GCATACGGGCTCCCGAGTCCTGGGCAGGGAAGGGGAGGGAAATTGCTGCTTC1520.1327499323150016No Hit
GCCTCCGGGGGAAGCTTCTTCATGGAGTAAAAAATGTATTTAAAAGAAAATT1490.13012986786141606No Hit
AGTAGTGGGAAGTGTTTCGTATCAGGATGGCCTCAGACCACGGGAAGGAACT1470.12838315822569235No Hit
TACTCAGGGGATCTATGATAGGGAACCAGTAACTAATATTTCAGCTGTTGAA1470.12838315822569235No Hit
GCACACGGGCGGGGACTCTCAGCGGCTTGACCAGAGTGAGGCGCTTCCAGCA1470.12838315822569235No Hit
AATGCTGGGATATCCTGACATTTTATGTTTCTGACATTTGTTTCACTGATTT1470.12838315822569235No Hit
TCTTGGGGGCGCTACTCAGGAGGAAATTACAGAGACAATTATGACAACTGAA1440.1257630937721068No Hit
AGGAGCGGGACTGCACAACTGCATGGAAACTGAACAACCTGCTCCTGAATGA1440.1257630937721068No Hit
TCTGAGGGGCCCCAATTCAGCTTAGGTGCCTGAGTTTGTCCTCACTTTGTAT1440.1257630937721068No Hit
CTTGGCTGGGCGATCTGCCGACGGGATTGTAGTTGTTCAGAAATATGCTTTT1440.1257630937721068No Hit
TCTGCAGGACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGC1430.12488973895424495No Hit
CCAACCGGGTCCGATCTCAGTCAGGGATGGCGATTCCACACCAAGTACATGA1430.12488973895424495No Hit
AGGTTTGGGACAGCATAAATCTGTTAGCATTATTGCCAAATAAGACAGTTGG1420.12401638413638309No Hit
TTAGTTGGGGCTGCACACTTTGCTTTTGTTAAACAGCAGGTAGTAGACAGAA1410.12314302931852124No Hit
TATTGAGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGG1410.12314302931852124No Hit
CCACACGGGGTGATGAAAAGGTTTTGGAACTAGATAGAGGTGGTGGTTGCAC1410.12314302931852124No Hit
GCAAGCGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCC1410.12314302931852124No Hit
TAACGCGGGGAAGAGGGCACCTGAGGAGACTTTGGGGACAGGGCAGGGGCAG1400.12226967450065938No Hit
TAGCTGGGGATGCCTTTCCTAAGAACTCAAAGTTCTCTTTTCAGCGTGCTGT1390.12139631968279753No Hit
CGGCTGGGGGTCAGTGCTGACAAATAACATTTAACAAGTATTGCAGTAATCA1390.12139631968279753No Hit
TGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAG1380.12052296486493569No Hit
TTACGAGGGGTAGGTTCAAAAGGTTTCGAGGTGGCACCTAACCAGAATGTGG1380.12052296486493569No Hit
TATAACGGGCTGTCACTTGCAGTACCATAGCTAAGTCTGCTATCTCCAGCTA1380.12052296486493569No Hit
GTGACAGGGATACATGGAATAGTTATATTTTAATTAAGCATTTATTTTAGTT1360.11877625522921198No Hit
TACACAAGGGAAGAGGAAGACAAAACAAAACAAAAACCTCAAAACCAAAAAA1360.11877625522921198No Hit
CATTGCGGGGTCTTTTATAAATTTAGTAAATCAGTTTATAATCTTTAAAGCC1360.11877625522921198No Hit
CAGCTGGGGAACTGATAAGAGTGGAGGAGGAATGCCTTCTCCTAAGAGTCAG1350.11790290041135013No Hit
ACTGTGGGGTGATGCATTTGACCACATTACAATTAAGAATTTCTATTCAGCA1350.11790290041135013No Hit
GATGCAGGGATTTCAAGGAGAAGCAGTGTGTGAGCCAGTCAGTTCCTTCTCA1350.11790290041135013No Hit
TTTACAGGGTACTCAAAGACATAGATGTGAAAATTTTAGGCAACCTTCTAAA1340.11702954559348827No Hit
GAATCCGGGCGCCCAGCCGGAGGGCCCCGGGGGTGCTGCGAGCTGGGAATAT1340.11702954559348827No Hit
TGACCAGGGCGATCTCAAGACGGGAGAAGAGATAAGACATATACACAAATAG1340.11702954559348827No Hit
CTTAGGGCTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGCTTAGC1340.11702954559348827No Hit
CGTATAGGGATTCTCATGAAATGTGCTTGGTCTGTGATCTCTTGGTCAGATA1330.11615619077562642No Hit
GGTGGTGGGATATGATTCACTCTCTTTGTTCCAGAGCAGATACAAATTATCA1330.11615619077562642No Hit
ATAACTGGGTAATTTTTACTTATTTAATGAGCTTTTCCTCTCATGGCATATT1330.11615619077562642No Hit
TCACCTGGTATTAAAACTATTTACTGTTAAAAAATCTGTGACTTCATGAAGT1320.11528283595776458No Hit
TAATGAGGGCATCCCAAAAGTTATATACACAATTGCCTTTAAAACCACAAGA1320.11528283595776458No Hit
AAATCAGGGGGGCAAATGGATAACTTAACCAGTCCATATATGGTTTTAAAGT1320.11528283595776458No Hit
CATCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1320.11528283595776458No Hit
CGCTATGGGACTTTTATCTTTGTGAATAATTTACTGTCCTTTCCTCCTGGGA1310.1144094811399027No Hit
GGCTATGGGAACGATCTTGCATTCTGGATTCTTGCAGCAAAGTCTCAGATAC1310.1144094811399027No Hit
AGGCTAGGGATGGACGTCCTCCCCTCCAAATCCAGAAGCTCCCAGAAGGTGT1310.1144094811399027No Hit
GGTGCAGGGAAGGCTTCATGGATACATGCTAGTATCCATGTTCCTCTATTCT1310.1144094811399027No Hit
ACAAGCGGGGGGCCTGGCTACATGTTCTTCATTCCTGTTTAATAAATCTCAT1300.11353612632204084No Hit
GCTTTCGGGGTCTACAGAAAAATGTTCCTCATGAACATAGATATAAAACTTC1300.11353612632204084No Hit
CCATACGGGGATCTAAGCTCGGGATCAAGAGAATTGAAGAAAAAATGTTAAG1300.11353612632204084No Hit
CAGAGAAGGGAAGGCTGGAGATGGTCCCAGATCCAAGATGTCCCAAAAGCCT1290.112662771504179No Hit
CTCAACGGGCCATGAGTCTTTCTCCTATATTGTGAGCATTGCGTATTCCAAA1290.112662771504179No Hit
TAGCTGGGGACCCTTTAGGTTTTAGGAGGCTTGAGAATGGAAGGATCCTCAT1290.112662771504179No Hit
GCGATAGGGGGTATAGATTGCTGGGTTATGTATATTTCTGCAGTGCTTCTAT1290.112662771504179No Hit
GGTGCTGGGAGAAATGTTCATTTTTGTTTTAAAATTTGTTTTCCATGTGAAG1290.112662771504179No Hit
ATTGCAGGGATACTGGATCAAAGAAGAATTAATAGGGAAATTAGAAAATAAT1280.11178941668631714No Hit
TAGCGCGGGATTTCATTTCTTTGATAAATTACATTAAAAAATTAATGAGAGT1280.11178941668631714No Hit
CTGCAAGGGATCTTCGTAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTT1280.11178941668631714No Hit
TAGTAGAGGGGGGAATTCAGCTAGGACTAGGGGTGAGTGGTTAGTTAATGGA1270.11091606186845529No Hit
AAAATCGGGCTCTGTTCAGAAGTTCTGTCTATATGTGGGAAGAATGAAAGTT1260.11004270705059344No Hit
GTCAGTGGGCAACTACTTAAAAAATATAGTCAATAGGTTACTAAGATATTGC1260.11004270705059344No Hit
CTATATGGGACTTTTTATTGTGCATTTTTATTGACACTTTAATGACTCCCCC1260.11004270705059344No Hit
AAACTTGGGATAGCGTGGATTCCCCGGAACAGCTGTCTGGAATAAGGATGAT1250.10916935223273158No Hit
CAAACAGGGAGACTAACTCAGAGACAGCTGTGGTGGCCCTCACTCAGGACCA1250.10916935223273158No Hit
TGACAAGGGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACAATTAT1250.10916935223273158No Hit
CGCCAGGGGGAATTACAGTTCATGCATTAGCATTTTTAAGTGAAGTTCTCTT1250.10916935223273158No Hit
CTTCTTGGGACAAATTAAATATAAAAGTGTGTCTTTCTACAGCCAGTACACT1250.10916935223273158No Hit
TTGTGTCGGGAGTCTGAGTTATGATACCACTTGTGGTGTATCAGTTGCTTAG1240.10829599741486974No Hit
TCAGGTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1240.10829599741486974No Hit
AGGACAAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG1240.10829599741486974No Hit
GTAAATGGGCGCAGGACAGCTGCCCACAAAAAGGAGTCCAGCCTAGGAATCC1240.10829599741486974No Hit
AAACCTGGGTGACTGGCGTCTCTTTCGTAACATTTTTATGGCCTGGTTTGTC1240.10829599741486974No Hit
TTGTTTGGGTTGGAGTCGGGATTCCCCTTTCCCAAACATGCGTCTCGCCACT1240.10829599741486974No Hit
CAGTTAGGGAAGTGTAAATATGTCTATATGGAGAAAGCACATTATATTTATT1230.10742264259700789No Hit
GATCCCGGGGTAGGAGTTTGGGCAAGTTAAAAAATCAGAGCTAAGTCCTCAC1230.10742264259700789No Hit
GCAGTTGGGTCTTTAGATCTGGCTGTCATAATTTATCTGTTTTGCTTCATCC1230.10742264259700789No Hit
GCTTCAGGGTGCATTCATCATGGCTGCCGGCCCAGCCTCCTTCTGCTGCCAC1230.10742264259700789No Hit
CCCATAGGGAAGTACCTAGGGGAGAAAGAGCCATGTAAATATCTGTAATAAA1220.10654928777914603No Hit
AGCTATGGGTGCTTGTGCGCAGGGATCGAGACGGGCAGCACTCATGGGTGCC1220.10654928777914603No Hit
GAGTTGGGGATGTCCTCACATGGTAGAAAGACCAGAGGGTAGGAGGGCCTAC1220.10654928777914603No Hit
AATGGCGGGCAAGGGTGTACGCAAAGCACTCATTGATACCCTTTTGGATGGC1220.10654928777914603No Hit
TAAAGTGGGAGACAGGGATGAGGGTCATTGATCCTGGGTGCAGATCTCCAAA1210.10567593296128418No Hit
TTCTAAGGGGAAATTTATAGATGGGACAATGCACATACTTCATATGTAAACA1210.10567593296128418No Hit
ACGTTGGGACAGTGAAAGAGTAAATTAGAAAGTGAATGGATGACTGTTCATG1210.10567593296128418No Hit
TTTACGGGGGGCTAACCAAGAAACTAAAGAAGACTTACCTGCGGAAAACGGG1210.10567593296128418No Hit
AGAACAGGGACTTTGAAAACGACTGCTGGGTCAGGTACTTCCTGCACACAGG1210.10567593296128418No Hit
AATTGTGGGGTTGTAGTGGGATGAAATGAGGGAGTAGCTGTAAAGCTTTCAG1210.10567593296128418No Hit
TAGATGGGAGTGAAAGAGTAAATTAGAAAGTGAATGGATGACTGTTCATGAG1200.10480257814342232No Hit
TGTTTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1200.10480257814342232No Hit
TTATTTGGGGTCCTTTCATGTAATGTCTGATTTTTATGTGGAAGAGAATGTA1200.10480257814342232No Hit
GACATTGGGACTGTTAAGAGTCCTTTTGAATTAAACTCGACTAAAACAAGTG1190.10392922332556048No Hit
TGATAAGGGGGGCCACAGTAATTACAAACTTACTATCCGCCATCCCATACAT1190.10392922332556048No Hit
TCTACGGGGATTGCCTGTGATCTTTACGCTTAACTGTTGTGTATCTTTTTTG1190.10392922332556048No Hit
GCGCAAGGGATCTTTTTGGAGATTTATCCAAGTGCCAGATTACTCAGTGCTA1190.10392922332556048No Hit
TCAGCAGGGACATGTAAGTAGAATAATACCACAGATGATTCTGCCTGGAACC1190.10392922332556048No Hit
TAGCGTGGGGAGACTTTTCATTTTGTGGGAGAGTAGCAGCAAGTCCTTGGAA1190.10392922332556048No Hit
AACAGTGGGAGCCACATGGTGTGAACCATTCGATTCTCACAACAGAATGGAT1190.10392922332556048No Hit
GTGAAGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1180.10305586850769863No Hit
CTAGTAGGGTGATCTAATGCGTGAAAAGAAAAAACTGAAGAAAAAACTTGTC1180.10305586850769863No Hit
GATAATGGGATAGTCAAAATGTGGAGATTATTGATATATGAAACAGAAGCCA1180.10305586850769863No Hit
CCTGATGGGTGTCTTCTTTTCTCTTGTATAATCAAAGAAATGCAAGCATGAG1180.10305586850769863No Hit
CACTTCGGGAAGGAACGGCTCAAAGCATACACATGGGAGGGAGGAGTGGGGC1170.10218251368983677No Hit
TTGTGCCGGGAGGAGGCATAGTGATTCTTAAATATTTACGGAACATTTGGTG1170.10218251368983677No Hit
ACCAGTGGGACCTAGGACAATTATTTGTTACATAATCCGACCTCATAGCAGC1170.10218251368983677No Hit
CGCTAAGGGGTTCCAACAATCAGCGAAGTAGGAAATGTTTCGGAGACACTAA1170.10218251368983677No Hit
GTTACTGGGATCTAATAGATTGATAAAGGAGCTTTCGTCCACCACAGGTGGA1160.10130915887197492No Hit
TGTGGTGGGCTGGGGTATATCCTGTCTGTGTTCCCAGCCACACTGCAATGAC1160.10130915887197492No Hit
TAGTTGCGGGGGGAGTCGAGCCAGCGTCGCCGCGATGGTGTTGTTGGAGAGC1160.10130915887197492No Hit
TTCCATGGGGTGGCCAATTATCGCAAAGCTGGCCCGAGAGGAGATGGATCGG1160.10130915887197492No Hit
AAACAAGGGAAACAACAGATGCTGGAGAGGATGTGGAGAAACAGGAATGCTT1160.10130915887197492No Hit
ATATCAGGGTTACATTAATAACCTTTCACCTCAGGGTTTTATGAAGAGGAAA1160.10130915887197492No Hit
TGGTGCGGGCACTTGGACATGTGTCCCTGTTACCTGAGAATGACAGATGGAA1160.10130915887197492No Hit
CTAACCGGGCTGGGCAAGCTACCTCGAACCCTGTCGTTAATTACTTCTGGTA1150.10043580405411306No Hit
TTGTAAGGGAGTTTCATGGAGATCGAAATACCAAGGGACATCCAATGGCAGA1150.10043580405411306No Hit
GTCAGTGGGGGGCAATCCAGTAGTAGATCTCTTTAGGTATGTGTATTAGTCC1150.10043580405411306No Hit
GTTCTGAGGCAAAATCTTTCCAAATCAATTATATTTTGCTGCTTTTCTTTAA1150.10043580405411306No Hit
CAAGGAGGGAAGACAGAAAGACAAACCAAAAGAAAACAAAAGTAGAGAGAAA1150.10043580405411306No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)