FastQCFastQC Report
Sat 31 Dec 2022
SRR5845558.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845558.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences175646
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCATGCACATCTCGTATGC6110.34785876137230565No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATGCACATCTCGTATGCCGTCT5260.29946597132869524No Hit
TTTTGCGGGAAATGTGCAAATACAAATATACACACACACATATTTTCTTTTT2850.16225817838151738No Hit
CTCGTTGGGGGAGCGGGTGGTGGGCGTCTGAAAAGTCGCCACCATGAGGAAG2640.15030231260603713No Hit
CAGCAGGGATGGTTTCTGGGGCCAAAATTCCCTTTCCTGCCTCCCAATAGCT2620.14916365872265808No Hit
TGTTTGGGAGAAGCAAGCTGAGAGTTTGGCATATCGAAGAATAATGTTTTAC2610.14859433178096854No Hit
GGTCCTGGGTCAAGCAGGTTCTGTGAATTGTAGGGACCAGAGAACATTATTT2600.148025004839279No Hit
AATTCAGGGCAAAGACATTAAAGATTTTTGTTCAACACAGGCACAATTGACA2520.14347038930576272No Hit
CGAAATGGGGACTGGACTGTTGGTAACAAAGTAATTGTGAGAACCATGTCGG2490.14176240848069413No Hit
TCGACCGGGCTGACATGTTAACATTCTTAGATTTTCAGTTTATAGAACTTTA2480.1411930815390046No Hit
TTTTATGGGTCACCGCCTCAGCCATGCGAGTCCACAAAGATGGCTCCCTGTG2470.14062375459731505No Hit
GTAGTGGGGATGAGAGTACATATTGAAATTGGACCCGATGGCAGAGTTACCG2460.1400544276556255No Hit
CTGTCTGGGAACAGACACATGAAAAAATGCTCATCATCACTGGCCATCAGAG2450.13948510071393597No Hit
CTAACACGGGGAACTTTTTGGTTGCAAAACAGAGACATGGTTCTATAATGCT2450.13948510071393597No Hit
GAAATAGGGCATAATATTATTTTGGTTAAATAATGACTGGCACAGAGTAAGT2430.1383464468305569No Hit
ACGTAAGGGAAAACTTGTTGATTGCAAAATACAGTTCTATAAACAAAACCCA2410.13720779294717783No Hit
GAGGCGGGAGATAGAGCCAGATTCATTATTGCACATAAGCACTCTTCAGTGG2370.1349304851804197No Hit
TGTAGAGGGTACTGCATTTCAGCATACTCCAACATCAATTACTTTAACTGCT2370.1349304851804197No Hit
CTATCAGGGATAGACTGACTCTATACTTACTTGCCTCAATAACATTAAGACA2360.13436115823873018No Hit
CAACTTCTTCCTCCTCACACCCACATTTTCTCAGTTGCACAGCTGAGGTAAT2340.1332225043553511No Hit
GGTGCTATTTTGCAGTTTTGTAAGTGGATTTCCTAGGGTCTGGAGAGGGAAC2340.1332225043553511No Hit
TACACTGGGAGTATGTAAAAGCAATAACATTGATTCTCTGTTGTACTTTTTT2330.13265317741366156No Hit
CGTTTGGGGGTCATTTTCTCCCTCTACTCACAATACTAAAGGGAAAAAATGG2330.13265317741366156No Hit
GGGCGAGGGATCTTGTCTTTGACTAGAGCTTGAAACCTTGTTATCTGATTGT2310.1315145235302825No Hit
TCAATGGGGAATCGGCTACAGTCTCAAAGGGCAATGCTTCTGCAGGGCACTG2300.13094519658859297No Hit
ATTGTGGGGAAGTGTTTGTCAGTGGGTGAATGGATAAAGAAACTGTGGTACA2290.13037586964690343No Hit
TGTACTGGGAGATGTGTTGAAGCATCCATTGCATCCATTTTTTATTATTTTC2290.13037586964690343No Hit
CAAGGTGGGGACACGGGGATGTGGAGATAGTTAATGGGTACAAAAAAAAGAA2290.13037586964690343No Hit
TTATTTGGGGATTTAGAGTTCAGAAAACCAGTGTTAAGTTACTTGTTTGACA2280.1298065427052139No Hit
TCGTACGGGCACCTGGCTGGGTATTTTAACAATACAAGGATAAGGCGTCATC2270.12923721576352437No Hit
TTCCTTGGGGCCGGGAAGATGAGCACGGAAGGTGGTGGCCGTCGCTGCCAGG2270.12923721576352437No Hit
GGCGGTGGGCGGCCTCCTGCACCATGTCGGTGGCCGGCCTCAAGAAGCAGTT2240.12752923493845578No Hit
TTGGCTCGGGATGGGCTCCACAGATCAGCCCATTGCCTAAGGCAACAGAGAG2240.12752923493845578No Hit
GCACATGGGTTCATGCAAGAGACGAAAAGAGGAATGTCATGCTGGAAAGGTG2220.1263905810550767No Hit
CAGGCTGGGGTTAGCCAGGATGGTCTTGATCTGACCTCGTGATCCACCTGCC2210.12582125411338715No Hit
GCATTTGATTTTTTATTATTTTATGTTCCCCCGTTAGATTGTTTTAAGTGTT2210.12582125411338715No Hit
ATCTAGGGAGGGCACACATTCCTCAAAACTGAGGTCCTTGGAGAAAGAAGTT2180.12411327328831856No Hit
TGCAAGGGGTCAATGTCTAGATAAGTTGTGCCTTATAACCAAAAGTGTCTAA2170.12354394634662902No Hit
TACATGGGGAGTGAGCAAAGAGGACGCCATGCCGGAAGCACTGAAAAGTCTC2160.12297461940493946No Hit
CGCCATGGGAGTGCGGGCTGGCAGTGCGGGCAGAGCCCGGCTGAGAGGGGCG2150.12240529246324995No Hit
TCGGCCGGGCTTGCCAAATGCTTTTTAAAAAGTTGAGTATACATACACATAT2150.12240529246324995No Hit
TTTAGAGGGCATCCACGTGATTTCTCAGGATAATCTGGAACAAAGATAAGCA2130.12126663857987088No Hit
TACAACGGGCAGCAATGGCCAATTGAGTTGTTTTCATGCTGTATCACTCTGA2120.12069731163818133No Hit
AGAGCTGGGCACCAATAATAGACAAACAGAGAGCCAAATCATGAGTGAACTC2110.12012798469649182No Hit
TAGCTGGGAAAACTTCAACATTTTCATAATACAGTATTAATGTTTGATAAAG2100.11955865775480226No Hit
TTTAGCGGGAGCAGATGCTGGCCATCCAGAGCAAGAACAGCAGCTACTTCGT2100.11955865775480226No Hit
ACTTCAGGGTGATGAGGTGAAATGACATAATGAAATGATGAAATGGAATAAT2090.11898933081311275No Hit
ACCACAGGGGGCGAGACTCGGCGGGCGCTGTTGAGGGAGTCGGGCCGCGACT2090.11898933081311275No Hit
TTTGCGGGGACTAACATGCTGTTTTAGCTTTGTATTAAATATAATCTGGCAG2090.11898933081311275No Hit
CTTTGATTGGGATATTTGTAAGGATTAAGTGAAATAACGGGTATGGCAGAAT2080.1184200038714232No Hit
TACTGCGGGATAGTGGATGATTACTGATTTAGGTCCAATAGTCTGTGATGTC2080.1184200038714232No Hit
GGCTCGGGTGAAATTTGTGTTAAGAATATACTCCTTTTGGCCTTTTGGCTTT2070.11785067692973367No Hit
CAAAAAGGGGTGGAGGAGGAATGCCTTCTCCTAAGAGTCAGTTGAAAGAAAG2060.11728134998804413No Hit
AGAAGTGGGGCACTTTAAAAAAAATAATTGGTTGACTTAATATATTTGCTTT2060.11728134998804413No Hit
ATCTTGGGGGACATTCCCTGGGTCCTTCTCAAGAATGAACTGGAGCTGGCGG2050.1167120230463546No Hit
GGTCGCGGGCATGGATTTTGCGGTGTTGTATTTACATTTTCAATTGTTTAAA2040.11614269610466506No Hit
TGTATTGGGATTTTAAATGGTGTTTTAGAAGCACTTTGTCTACCTAAGCTTT2040.11614269610466506No Hit
GTTCAAGGGGGTCACCCTGAAGTTTATATTCTTATCCTACCAGGCTTCGGAA2040.11614269610466506No Hit
GTCGCAGGGAGTGCTGAATATCAAGAGTGTACATATCATCCAAAAGAGATGT2030.11557336916297553No Hit
AGCTATGGGAAGGGAGGAAAAAGATGTTAAACTTTGTTTTGTAAATGCTAAT2030.11557336916297553No Hit
CCATTCGGGGGCGCGAGCCGGGAGTCCCCGCGGCGGGCGGCGGTGGCGTTGG2030.11557336916297553No Hit
CAAACTCCTGACCTCAAGTAATCCACCTGGCCTGCTCTTTTCATGTCTTAAC2020.11500404222128599No Hit
AGAGGGGGAGTGAGACCTTGTCTCTATAAAAACAAAAAGCCTCCTGCAGATC2020.11500404222128599No Hit
GAGTGGGGGATTTAATAATATTGTAATTCTGTATTTCTTTGGGGGGAAAAGG2020.11500404222128599No Hit
TCGAGAGGGACAGATAATCCTAGGGAGTTGCAGGTCAAATATCTAACCACTT2020.11500404222128599No Hit
TACCATGGGGTATATTCAGAGTACCAGATAAATGGGATTTTTCACTAGGGGG2010.11443471527959646No Hit
TCGCCCGGGGGCGGGGTCGACTGACGGTAACGGGGCAGAGAGGCTGTTCGCA2010.11443471527959646No Hit
TTGCCTGGGACAGATTAAGTAAACTGAGTCTTAAAGCTAAAGTGACACAATC2010.11443471527959646No Hit
CACTACGGGGAAAGGCCATCTCTTCCCTGAGCCTTCATATCGGCTTCCCTTT2010.11443471527959646No Hit
GTTTTGGGGGATCTTCCGGTGGCATGGAACCGGATGCTGCAAACCCAGAATG2000.11386538833790692No Hit
GTTCAAGGGAGTGAGAAAGAGCTGAAAGCAGGAGAGTGTGGTGACTGCGGAA2000.11386538833790692No Hit
GTCTCTGGGGGGGAGGATGAAGTTCGTGTACAAAGAAGAGCATCCGTTCGAG2000.11386538833790692No Hit
GCACTCCGGGAGGAGGCTGAAGGAAAGAATCTGTGACTAAGGATTTTATGTT2000.11386538833790692No Hit
TACCCTGGTTTGAGCCATGTATTACAAAAGTATAGGACGTGTGAATATAAAT2000.11386538833790692No Hit
TCTGCTGGGAGCACAAAACAGCAGGGCAAAGAATAGTAGATTTGATCCTGAG1990.1132960613962174No Hit
TATCCTGGGAAGCCACTCCTGGATTTTTCCCTAAGGTAATCCTCTGCTTAGG1990.1132960613962174No Hit
TAGAGTGGGATGGCATCACAGGAGCGCCTGTAGGAGGGAATAACGGCCTTTA1990.1132960613962174No Hit
TGGTCTGGGGGTTGATACTCGGAACATTAAACTTAGGTCCCTGTAGGAGTCC1980.11272673445452785No Hit
GATATTGGGAAGCAACCCAAATCTGAAATATCACTAATTAGAGAGTTCTTAT1980.11272673445452785No Hit
ATTGATGGGAGAATTCACTTACCTTGGATCAGTTAAATAACTAGTTGATTAG1980.11272673445452785No Hit
ATTCATGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG1980.11272673445452785No Hit
AAGCTGGGGTTGGAGACGGGGTCTTGCTCTGTCAGTCAGGCTGATACGCAGT1970.11215740751283833No Hit
GATCAAGGGCTTTTCTTATAGTTTTTTATGCTAATTCTGTTTCATTTCTATA1970.11215740751283833No Hit
ACAGAAGGGCGATCTGCATGTGGGATGCTTTGAGTGGAATACAGTCTAGACT1970.11215740751283833No Hit
CGCAAAGGGCAGGATATCACTTATTAGATTGTAGACAAGGAAAATGTACATT1970.11215740751283833No Hit
TCACGCTGGGGTTGAGGAGTAGCTCATTGGCATGAAGTAGACTTAAATCTTG1960.11158808057114879No Hit
CTTCTGGGGCTGTTCTCTAAGAAAACTTTTATGAGCTTTAAAGTTATGTGGT1960.11158808057114879No Hit
GCAAAGGGGGGGGCCGCGGCGGGACAAATGCAGAGCGCACGCGCTTGGCTGC1960.11158808057114879No Hit
CTAACGGGGAGGCCATTTTCATTTCACTGAGAGATCTAGGAATAATGCCTTT1950.11101875362945926No Hit
ATGTATGGGCACGGAGTTGCATGCAAACTTCTGTTGTTTTGTTAACAGTGTT1950.11101875362945926No Hit
AGGCGGGTGATGCCAATCTCAACATTGTCTTTTTAATACTGTCACCAGATAT1950.11101875362945926No Hit
TTAGCAGGGCGCCCATCACCACTACCACCACGGGTGGTTCGTCATGATTGCA1940.11044942668776972No Hit
AGCCTAGGGGTCTGAAACAAGCCTAAATAGAATTCAAGATTAGACTAAATGA1930.10988009974608019No Hit
TCTTGTTCGGGGATTTAGAGAGAAATTAGTGAAACCAGGAATACATCATGTT1930.10988009974608019No Hit
TAGAGCAGGGTGGTCTCTTCCAACTTCATGTACCAGCGTAAAGATTAAAGTG1920.10931077280439065No Hit
GGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAAGA1920.10931077280439065No Hit
TTAGGTGGGATCCTTAGCCATAGCGAGCGGTGGTGGTGGATAGCATCACAAG1920.10931077280439065No Hit
GCAATAGTGGACTCATAAATCCTTCTTAAAGCCTTGATCGCACTTTGAGTGA1910.10874144586270112No Hit
TGACCCGGGCCGCGCCGGGCAAGAAGATGGCGGGATCAGTGGCCGACAGCGA1910.10874144586270112No Hit
GAAGTAGGGCATTCTAGCCCTGGGTTGACAGAGTGAGAACCAGTTTCATAAA1910.10874144586270112No Hit
TGACAAGGGACTTGCCATATAGTAGGTGTTTAGGACATGTTATCTTCTTATA1900.10817211892101158No Hit
GCTCTTGGGCGGCGAGGGCCTCACCGAGGGCATGGACAAGCTCTATGAGATG1900.10817211892101158No Hit
TACCAAGGGACCTATGATTTTGGGAGTACACAACTCAGCTCCTGACAGTTAT1900.10817211892101158No Hit
GCGCATGGGTATCTTTTAAAATACACCTACCTCATACTTTGTGTGAAGTTTA1900.10817211892101158No Hit
ATAGAAGGGGTATTTTGGTATTATAAAATTATACTTCTTAGATTCCGTTAAG1890.10760279197932206No Hit
TGCCGCGGGCCGATCTCTTAGTGGGACCGTCAATGCTCCTTACCAGTTAGGG1890.10760279197932206No Hit
GCAGCGCGGGGTCCCAGGGTGGAACTCCACATGCTGGTGCATATACGCCCTT1890.10760279197932206No Hit
TGGACTGGGGTGACAAGGATGCCCTGGAGGACCACATGGATGGACACTTCTT1880.10703346503763252No Hit
GGGTTAGGGGAGAAGCCAGAACTCAAAGGAAGACTATGAGAAAAAAATCAGC1880.10703346503763252No Hit
CTTATTGGGGGGCTTGTGTATTATAAGCTGCTGATATACTAAATCCTTATGT1880.10703346503763252No Hit
ATTACTGGGATCACAGACTCTGTGCAGATAAGCCCCAGTACCAGCCTAGAGC1880.10703346503763252No Hit
TATTAAGGCGGAGACTGAAGACCAGGCGGTGGAGCTGGGGAGGGTGGGAGCA1870.10646413809594299No Hit
TTGGCAGGGGAATCGGACTCGGACAGTGAGAGCAGTCGGTGTCCGCTGCCAG1870.10646413809594299No Hit
GATTAGGGGGGAGTCAGGGAGCTCTCCTGTCACCAGTATTGATTTCAGAGGA1860.10589481115425345No Hit
CTGCAAGTTGGGAAGATGATAAAGTTACAGAAGCATCTTCAAACAGTTTTGT1860.10589481115425345No Hit
TGTTATGGGATCAAAAAGATAATCCACCATGATCAAGTGGGTTTCATACCAG1860.10589481115425345No Hit
GAACATGGGAGGCTTCTTCCTCTTACATGCATGGAGGAAATCCCTCTGGTTC1850.1053254842125639No Hit
ACCCAGGGGGAGTGATAAAAGGCAGAGACCTACTTTCTGAATATAGGCATTC1850.1053254842125639No Hit
TTTCAAGGGCTCCACTCCCAACCTTTCTGCTTCTCATCAGGCTAATCATTAT1850.1053254842125639No Hit
TGAAGAGGGATCAATGCTATTTATTGTACCTCTGGGCCTACTCTTCTAAAAA1850.1053254842125639No Hit
CTAACACGGGCGGACTGATAATTATGTGGATTCAGCAAATAGGATATAGACA1840.10475615727087437No Hit
AGAACCGGGGATCTCTAAGCGGGCTGGCCAACATGGGGGGGCTTGCGGCCGG1840.10475615727087437No Hit
GTAACAGGCATGTCAGTTGGCATCCATTTCTTCAGTAAGGTGAGAAGGTAAG1840.10475615727087437No Hit
AATCCTGGGAGATGAATCCAGGAAAAAAAATTCATGTCCTAGTTTGTTTGTT1840.10475615727087437No Hit
GGGGATGGGGTGTGAACACCTCAATCTCTTGGAGAAAGACTACTTTGGACTT1840.10475615727087437No Hit
ACCACTGGGAAGGAATGGAAGATCCATGTAGACGTGTCAGTGTCCAGGAAGG1840.10475615727087437No Hit
CTATCAGGGGGAATTGAAAATTTTGGTATGTATCTTCTTAGCCTTGCTACTT1840.10475615727087437No Hit
AGATATGGGAGATTGATCCCAACTATTAATACATTAAAGTGGTGTTGCATAT1840.10475615727087437No Hit
TGCCCTGGGTGACCTGGGGCAGATAAAATGCCAGTCTCATTGTCACCTCTGT1830.10418683032918483No Hit
AGCCTGGGGTGGGGAGTTTTCTAGTTACTAGACCATCTCCATTTTTAGCACT1830.10418683032918483No Hit
AGAACTGGGCTACCTCTCAATACCCTCCAGAACATCCTCCTCGTCTACCACT1830.10418683032918483No Hit
GCATGGGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1830.10418683032918483No Hit
ACACCCGGGTGCCTGTGTTCATTGTGGACAAAGTTATTATTTTGCAACATCT1830.10418683032918483No Hit
CTATAAGGGCAATGGACAATTTTCAAGGTGCTGATGTGATGTCACAACCTCC1830.10418683032918483No Hit
ATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG1820.1036175033874953No Hit
CGTAATGGGGAATCTCTTGCAGATATCATACAATTGGGTCTTGTTTCTTCAT1820.1036175033874953No Hit
TTAGCAGGGCTCCATGATTTTGGATATTTTGAAATGAGAAATTGATGGTTAG1820.1036175033874953No Hit
ATAGCAGGAATGTCTAACACCATTAGCTTTTGTGATCTGGTTAAGAAGTATT1820.1036175033874953No Hit
GTAATTGGGATATGAAAAGAGCATTAATGAAAATGCTTGTCATGAAAAGCTA1820.1036175033874953No Hit
TCAGCCTGGGCAACAGAGCGAGATTCTGTCTCAAAAAACATATATATAAATA1820.1036175033874953No Hit
CCATGGGGAAATAGAGTGAGTAACATGAAGAAAATATTAAACTGAAAAAAAA1810.10304817644580576No Hit
TATAGCGGGATACTTGAAGGAAATGTTTCACCTTATTTTTGGTCTTTGTTTA1810.10304817644580576No Hit
TCAATAAGGGAACTCACTATGTTTTTAAGAGCACACATAGGTTGAAAGTAAA1810.10304817644580576No Hit
GGATAAGGGGCTGTGGGTATGCATGTACAAGTGGAATTGCTGGGTCATATGA1810.10304817644580576No Hit
CTTCAAGGGCTGTCTCATCCTCCTGGAACCTTGCTGTCCTGATCTGTGATAG1810.10304817644580576No Hit
GGTCAAGGCGATCTAACAGAGGGCTTACAGAGCAGGTGCATAAAAGTAACGT1800.10247884950411623No Hit
GAATCGGGGCTACCACAAGGATTAAACCAAGGACTGCCACTGTACGAGCACT1790.10190952256242669No Hit
TGGCACGGGGAGTGCTTTGTAGCCTATGTTGCATGTAGCGGACTTTAACTGC1790.10190952256242669No Hit
TGCTAAGGGACTGTGGATAGATTTGTGCCATATTGTATAGAGACTCTTTTGA1790.10190952256242669No Hit
ATCTGGGGGGAGAAGGGAAACGTGAAGAAGGTGAAGATGGCGGTGGCCAGGG1780.10134019562073716No Hit
CCAAAGGGGGGGCCCTAGAGCACTTCCTTTCTCTTCACATTTCCAGTCGTTT1780.10134019562073716No Hit
AATGGTGGGAACGTTAAAGATGTTACTGGGACCCGTTTCCCTTGTGCATGAT1780.10134019562073716No Hit
TGTTTTGGGGATTCTAATGCAATTTCCTGTGGGTATGATTTCTTAGCAGAAA1770.10077086867904762No Hit
TACCAAGGGAAATACAGTGTGGAGAATGTGTTACTGTCATCCTTAAAGATTG1770.10077086867904762No Hit
GATTTTTTCTATCCTTAAGCCCAGAATTCTTTGTACAACATAGTGCTGCTTG1770.10077086867904762No Hit
GTATAGGGGATGTTATAACCACTTGGAGAGCAATTTGTCCATACTTACCTCG1770.10077086867904762No Hit
CAAACAGGGGCCTCCCGAGTAGCTGGGATTACAGGCACACACCACCATGCCT1770.10077086867904762No Hit
TCTCCAGAACTGAGCATTTTCAAAGTTCCCGAGGAGAGATGGATGCCGCGTC1770.10077086867904762No Hit
TAAAGCGGGGCAATGGAACAGGTGAGAGAACCCAGAAATAAAGCTGCACACC1770.10077086867904762No Hit
CAGTGAGGGAAGATGTTAACTATAAACACACTGCATACAATTTTCTTCTGAA1760.1002015417373581No Hit
TTTATAGGGGTCCTCTTTTTCCTTTGTGTGCCTGTAATGGACACTTTTCCAT1760.1002015417373581No Hit
GTAAGCGGGGTGCCCTGTCTCTGCCTGTGTGTTTTTACTAAGAATTCCAGAA1760.1002015417373581No Hit
GCTATTGGGGAAGGAGGAGGAGGAGGAGGAAGGTCTTTCAACGACAAGGATG1760.1002015417373581No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)