FastQCFastQC Report
Sat 31 Dec 2022
SRR5845555.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845555.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences211381
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCATGCACATCTCGTATGCCGTCT6980.330209432257393No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCATGCACATCTCGTATGC6150.2909438407425454No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG3940.1863932898415657No Hit
GACACCGGGAAGAAAACACATTAAAAACACAGGTAAAAAGTAAAAAATAGAT3500.16557779554453805No Hit
CAGTTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA3110.1471276983267181No Hit
CTTTCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA3110.1471276983267181No Hit
TGCTGGGATTACAGGCATGAGCCACCTTGCACTGCCTGCCTCATATTATTTT3080.1457084600791935No Hit
GCATGTGGGGACGGCAATCTCATCTAGGCCACAAGAGAAACCTGATAAAAAT3030.1433430629999858No Hit
AGTGCAGGGCCATTTGGGAATTCAATACAGGAAACCAAGAAAGAGAAACCAT2880.13624687176236275No Hit
ACTCGCGGGGGCTTCTCTGGGAGCTCTCCAGTGGCACTGCTGGACCTGCCCA2880.13624687176236275No Hit
TATGGTGGGAAGCTTCATAAGTGAAGGAGAAATAAAATTCTTTACAGACAAG2850.13482763351483812No Hit
ATGTAAGGGGGGAACAAAACGAACTTTTTTCCTGGTTGAGCATTAACTGACA2840.1343545540989966No Hit
TAAACTGGGATTTGAACATATTTCTTCCATCATGTATTTTCTGCTCATCACC2810.132935315851472No Hit
TTACTAGGGACATGAAACTCACCCTTGAAAAACAATTCAGTAGTTTTTAGTA2790.13198915701978892No Hit
TAGGCCGGACGGCGTGTGTTGTGAAACGTGAAATAAAAAGAGCAAGAAAGAA2690.12725836286137354No Hit
GCACTTGGGAACCCAAGTCTTGAGCAATGTTTTTCTCAAAAAGCTGCTATCC2670.12631220402969046No Hit
CATCTTGGGGGTCTTTTGTAATAATGTTGGGGCACTTTAAACCTCAGAAGCA2670.12631220402969046No Hit
TTCGTTGGGGGCCATAAAGATAATGCTGCATTTTTCTGTCAGAATCACATAT2620.12394680695048277No Hit
TGGATGAGGGAGGAGGTGATGTCCAAGCCTAATTTTTACTCCAGCTATGAAA2590.12252756870295817No Hit
GTCATGGGGGTGTTAGGCAACAAAAAGTAGTAGAAATAAGAAAAATGTAATC2580.12205448928711664No Hit
TAGGTGTGGGCTTTTCGGCTGGAATGGGGAATTTGTGAGGTAGGCCGGAAGT2570.12158140987127508No Hit
TGCTTTGGGGGAACACTCAAAATTCCAGCAATGACCATTGCTAAGAATGCAG2550.12063525103959201No Hit
CGCATGGGGTGTCAGATAATGCTACTTTATCCTCTCATGGTTAACTAAAGTG2520.11921601279206741No Hit
GATTCAGGGATTGTTAATTGTGTTTAAACAAAGGCAAAGATTACTGACTAAT2510.11874293337622588No Hit
CAAATAGGGACATGGACCAAATGCATCAGAATTTTGAAGAACAAATGCAAGG2500.11826985396038432No Hit
TAATTTGGGTTGACTTCGCTCTTATCAAATGTTTTCCTATTTCTATTAATTT2490.11779677454454279No Hit
GGGGCAGGGCAATAAAACTAATAAAAATGAATACTGTATGGGACTAGAAGTA2490.11779677454454279No Hit
TGAGGGGAATGAGAAAAGAAAAAAGTCATGGTAGGTGAGGTGGTTAAAAAAA2490.11779677454454279No Hit
TACAGTGGGCATATCGTCAGTCCAGGAGCAGCTGAATCTAAGGATGCAGACT2470.11685061571285972No Hit
TCGGGGGAGGCCACGAAACTAGAAAAGTTCTTCATTATAACTATGTATAATT2460.11637753629701818No Hit
CACGGTGGGGGCTACAAGTCTGTTTTGTACATGTTACAGTTTTCGGTAATTA2460.11637753629701818No Hit
AGCGTGGGCATACATCAACATGAAGGTGTGACATCAGAATCATGAGCACCAT2450.11590445688117665No Hit
GAATTTGGGGAAAGTTTTCAAAGAACTCTATCCTATGCAGGGGCCCAGGATT2440.11543137746533512No Hit
GGCTCTCTGGTTTTCCGTGTTGTTGGGAACAGTACGTCCCAGGCTGTTTGCT2440.11543137746533512No Hit
GCTAAGGGGGGCCAGACTGTGCCAAAAAACGTTTTGCAAACCACGGGTTCAA2430.11495829804949356No Hit
TTCTTAGGGTCCTTGCAGAGGCCATGGAGGACGAGGAGAGACAGAAGAAGCT2420.11448521863365202No Hit
AGCCGGGTGGGGAGCATTTAATACTATTTTCACTATAGAAAATTATTAGTGG2420.11448521863365202No Hit
TGAACTGGGTATTTTTATTCTGTCATCTTGTGGTTTGGCCAAGATTGATCAG2420.11448521863365202No Hit
CGCATTGGGGAAAAATAAAGTAGCCAATTCTAAAAGTAGCCCTCTTTCTCCT2410.11401213921781049No Hit
CTCTTTGCGGGATGATTCATTCAACCAAGTCGCTGGACTTTTGAAGGCCTAT2410.11401213921781049No Hit
ACACAGGGGAGGAGACATGAATTAAGAAATTGGGAACAAATTAAAATAACAT2410.11401213921781049No Hit
TGCACTGGGGATAATTAATAATTGGAGCTGCAAAGCAGATCGTGACAAGAGA2400.11353905980196896No Hit
TTTTAGGGGTACCTCACCTTCCTGGGGAAACCTTCATAGACGCCTCACTGTT2390.11306598038612742No Hit
AAAAGTGGGCGATCTTCGGTTGGGCAAATAGATGAGTCGTAGTCTCAACAAT2390.11306598038612742No Hit
TCATACGGGTATTCAATTTTTCACTTTATTAGTAATGAGATGTAAGCTTTAA2390.11306598038612742No Hit
ACATATGGGTATTGCTTTGGAGTGTAATATTTTTAATGTATTCACTATTTCA2380.11259290097028589No Hit
GATCAAGGGATAGTTCTTCCTGCACTGTTTTCTTTATTTTCTTATTCATTTG2370.11211982155444435No Hit
AATAGTGGGCCGACCCCTACTTCTGTTTCTTCCTAAGGTATAATCTGCAGAT2360.11164674213860282No Hit
TGTGCAGGGGACATGAATCCGAATTCTACACTTCCAAATCAAATAGGTAAAA2340.11070058330691973No Hit
GCAAAAGGGGGGAGCCTTCCCGGATATTTAAGCTAGAAGATTCTACAGGGAG2330.11022750389107819No Hit
GTGGTTGGGGCTACTACGATGGCGATGAGTTTCGAGTGGCCGTGGCAGTATC2330.11022750389107819No Hit
ACCACTGGGAGCAGCCTTTTACCTCTGTGCTTTGTATGCTTTGTGTGTCTGG2320.10975442447523666No Hit
CTGTTAGGGAAGACTGAATCACTGAAAAGTGTAAGTCTACCTCTGAAGGAGC2320.10975442447523666No Hit
AATGAGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA2320.10975442447523666No Hit
CTCTAGGGCGGAAGTTGTTTTACCTACTTTAAAAGTTTTAATGGATTGCACC2320.10975442447523666No Hit
TTTGGTGGGAGGCGGGAGGATCACTTGAGCCAGGCCTAATCCTGAGCTTTTA2310.10928134505939513No Hit
ACTTTGGGGGGGATCAGATGTTCACCTAAGGCCTGGAGTTAATCTGGGGCCT2310.10928134505939513No Hit
AATTCGGGGGTCAAAACCCATCATAAGATCACTGTCAGAATTACTGGGGATC2300.10880826564355359No Hit
AGGTGTGGGGGTGACAGGGAGCTGCCTCCTCTCAAGAGCAGTTGGTTGGGAG2300.10880826564355359No Hit
GCTGGCGGGGTACTGTGCTGGGGCACATATCTTGTTTTCCCAGCTGCTAAAC2290.10833518622771206No Hit
GCTTAAGGGAATATCAGTCCTATGAGTCACTTTTCTAGCCATAATTTTAACT2290.10833518622771206No Hit
ACCTTAGGGATGTATCACTAACTCATATAGAATTCTTGTTTCTTATTCCCAG2290.10833518622771206No Hit
GTTTCTGGGCGATCTTAGGCGGGGGTCTTGGGAAATTTTATTATCTTCTGTC2280.1078621068118705No Hit
GTTAAAGGGATTGCTGCTTTCTCTTCTACTTCTTGTGCTTTACTCTGAATTT2280.1078621068118705No Hit
TATAGCCGGGCTTTTTTAAAATTGTGTTCTTAAAATATTGTGTAAGAATCAC2270.10738902739602897No Hit
GCCTGCGGGGGCGGTACGGCGTGTTGGTCCCAGCGGTTCAGCTGAGGTAGGG2260.10691594798018743No Hit
TACGGCGGGCATTACTAGAATTGTGACATTTACCCCTTTTTATATGATTAAA2260.10691594798018743No Hit
GCTGCTGGGGTTGGTTACAAAACAAATTAAATCTCTATTGTTAACTTTTAAG2260.10691594798018743No Hit
ACACAAGGGATGGAACTGATGGAAAAGAAACTTATGGATTACATTGATCAGC2260.10691594798018743No Hit
GAACGGGGGTTGCGGTGGGCCGGGATTGTGCCGCTGCTCTCCAGTCTGGGAA2250.1064428685643459No Hit
TATGCCGGGTACTGGGCCTGCGCTCCTTTGCAGAGATTGGCAGAGGGAAAAA2250.1064428685643459No Hit
AACAGCGGGAGAGCATTTCGGGGGGGGTGGGGGAAACACACCTAAACACTTT2250.1064428685643459No Hit
TACGACGGGTGAGATGAAAAATGAGATGAAATGATGAATTGAGATGAGATGA2250.1064428685643459No Hit
GCATATGGGAGACTACTGAAGGACTTGAGGATTCGTTTTCCTGGCTTTGAGC2240.10596978914850436No Hit
CAACGAGGGTTATTTGTTAGATGAGTAAGCAAGATACCATTGTCGTCATTGG2240.10596978914850436No Hit
TGGACAGGGTACAGGCGTGAGCCACTGCGCCCGGCTATTTTTTAAAACTATT2230.10549670973266283No Hit
GCTCGCGGGATTGCCACTCCTCACGATGGCCTATTTAAAGGGCGACCTCCGG2230.10549670973266283No Hit
TAGCTTCGGGGGCAGCCGGAGTAAGATGGCGGCGCTGAGGGCTTTGTGCGGC2220.1050236303168213No Hit
CTGTCGGGTAACGTGGCACTGGTAGGTTGGGACACCAGGGAAGAAGATCAAC2220.1050236303168213No Hit
GTAAACGGGGATCCAGCCCCCTTATAAGCCAAAAGCTTGTGGGCGAAATGCT2210.10455055090097974No Hit
GTGCAAGGGGGTTCTCTCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCAT2210.10455055090097974No Hit
GTGGGTGGGATCATTATTTTACACTAACATGGTGAGTGTTTTAGATTATTTT2200.1040774714851382No Hit
AAGTTCGGGAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTATT2200.1040774714851382No Hit
ACAACAGGGTCCAGACCTCAGAGTAAACCATAGCTTTTTATTTTAACTGGTT2200.1040774714851382No Hit
TGCATGGGGCTCTCCAGCTGTTATTTTCCTGAGGTCTGTTTCTTTATCTCTA2190.10360439206929667No Hit
TCACCAGATACCGCGCCTTAGAACAGAATAGATGCTTAGCAAACCATTACCA2190.10360439206929667No Hit
CGCGACAGGGTTTTGGAAGTATTTTATGAACTAAAAGTTAGCAAGCCCCCAT2190.10360439206929667No Hit
TGGTCTGGGAAGTATTGACAGCTACAAATTAAAGTCCTTAGAGCAGTTGACA2190.10360439206929667No Hit
TCAAAAGGGGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAGGAGGTT2180.10313131265345514No Hit
TTAGAGAGGGAATCCAAGAATTCTAAGGGAGGTACTAGCAAATAGTATCCAG2180.10313131265345514No Hit
ACGCAGGGACTTGCTGCTTACTCTGGCATGTCAATTATTGTGCCAGCTGGAA2180.10313131265345514No Hit
ATACTTGGGGCTGGCTTAACCATCTCTTTGTACAATCTGTTCTCTCTGTTCT2170.1026582332376136No Hit
GCAAGTGGGGGAAGACGGAAGCTCGGTTGATGTTTCTGCAGAAGTTTTCCCC2170.1026582332376136No Hit
TTGTTAGGGATGGCTCAGAAACATAACTGTCTTTGGAATCTAGTACAAGCTC2160.10218515382177207No Hit
TCACGTGGGAACAAAAGGAACTTTATAGTACGAAACGGTGGCAAACAGTACC2160.10218515382177207No Hit
ACTGCTGGGATTTTGCATGAATCCCAAATACAGCACCATTTTATTACCTATA2160.10218515382177207No Hit
CTGATAGAGGGATAACTCAGTATTTTAAACCGATAACACTGATTGCATAAAC2160.10218515382177207No Hit
GCTGCTGGCTATTTCTGCAAAGCCACTTAACTTTTCTTCCTGATTCCATTTT2160.10218515382177207No Hit
ACCGTCGGGCTAAAAATGTTGAAGGTACCTGACTATATTACTACCTGAAAGA2150.10171207440593052No Hit
ACGATAGGGGGTTTTCATTTCCTGGAAAATATTGTCTTTGTGGTCATTCAAC2150.10171207440593052No Hit
TCTCTAGGGCCTTCGGCAACCTGAGCGACTCCCGCCTTCCACGGAAGGGACC2140.10123899499008898No Hit
AGATGCGGGGGATCAGGGAGGTCACAGTGCAGTGTAGAGTGGCCGCTTCTCC2130.10076591557424744No Hit
GCAACTGGGCTACAGGCAAGCCAGTGAAGGAGGATAACGAATAAGGAGGAAT2130.10076591557424744No Hit
CGTTCTGGGAAATGAGTGTTATCAAAAAGAAACAAGAGAACAAGTGTTGGTG2130.10076591557424744No Hit
CACTATGGGATGTGTTTGGCTGGATAAATTCCAAATTTGTAACTAGAAGCAA2120.10029283615840591No Hit
CACGCCCGGGCTCTGTGGTTAGCGGGATGTGCTTTTTTTTCTTTTTTCTTTT2120.10029283615840591No Hit
GTACGCGGGTTTTCTAACTTTGTTTTGTCTTATTTTACCTAAGAACACTGGA2120.10029283615840591No Hit
GTTCTGGGGATTATTCCCATTTTAAACACAAAACTCTTAAAGCTCTAAGCAT2120.10029283615840591No Hit
CGAATGGGGTTTTCTAAATATACAATCATGTCATCTGCAAACAGGGACAGTT2120.10029283615840591No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)