FastQCFastQC Report
Sat 31 Dec 2022
SRR5845554.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845554.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212231
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCATGCACATCTCGTATGC5900.2779989728173547No Hit
CTTATACACATCTCCGAGCCCACGAGACTCATGCACATCTCGTATGCCGTCT5810.27375831052014077No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG3810.17952137058205445No Hit
GACACCGGGAAGAAAACACATTAAAAACACAGGTAAAAAGTAAAAAATAGAT3780.17810781648298316No Hit
GCACTTGGGAACCCAAGTCTTGAGCAATGTTTTTCTCAAAAAGCTGCTATCC3230.15219265800000942No Hit
AGTGCAGGGCCATTTGGGAATTCAATACAGGAAACCAAGAAAGAGAAACCAT3170.14936554980186684No Hit
TAAACTGGGATTTGAACATATTTCTTCCATCATGTATTTTCTGCTCATCACC3050.14371133340558165No Hit
TCACCAGATACCGCGCCTTAGAACAGAATAGATGCTTAGCAAACCATTACCA2950.13899948640867735No Hit
CTTTCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2900.1366435629102252No Hit
ACTCGCGGGGGCTTCTCTGGGAGCTCTCCAGTGGCACTGCTGGACCTGCCCA2890.13617237821053474No Hit
TGCTGGGATTACAGGCATGAGCCACCTTGCACTGCCTGCCTCATATTATTTT2820.13287408531270173No Hit
CAGTTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2820.13287408531270173No Hit
TAATTTGGGTTGACTTCGCTCTTATCAAATGTTTTCCTATTTCTATTAATTT2800.13193171591332084No Hit
GTCATGGGGGTGTTAGGCAACAAAAAGTAGTAGAAATAAGAAAAATGTAATC2790.13146053121363044No Hit
ACATATGGGTATTGCTTTGGAGTGTAATATTTTTAATGTATTCACTATTTCA2760.13004697711455915No Hit
AATGAGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA2720.12816223831579743No Hit
TTCGTTGGGGGCCATAAAGATAATGCTGCATTTTTCTGTCAGAATCACATAT2720.12816223831579743No Hit
CTCTTTGCGGGATGATTCATTCAACCAAGTCGCTGGACTTTTGAAGGCCTAT2710.12769105361610697No Hit
TATGGTGGGAAGCTTCATAAGTGAAGGAGAAATAAAATTCTTTACAGACAAG2710.12769105361610697No Hit
TGCTTTGGGGGAACACTCAAAATTCCAGCAATGACCATTGCTAAGAATGCAG2710.12769105361610697No Hit
CATCTTGGGGGTCTTTTGTAATAATGTTGGGGCACTTTAAACCTCAGAAGCA2690.12674868421672614No Hit
ACTTTGGGGGGGATCAGATGTTCACCTAAGGCCTGGAGTTAATCTGGGGCCT2690.12674868421672614No Hit
AGTAGCAGGAAGATACAAGAGAACAAAAAGATAGTAAAATAAGCATTCTCTA2660.12533513011765482No Hit
TATAGCCGGGCTTTTTTAAAATTGTGTTCTTAAAATATTGTGTAAGAATCAC2630.12392157601858352No Hit
GCCTGCGGGGGCGGTACGGCGTGTTGGTCCCAGCGGTTCAGCTGAGGTAGGG2630.12392157601858352No Hit
ATGTAAGGGGGGAACAAAACGAACTTTTTTCCTGGTTGAGCATTAACTGACA2630.12392157601858352No Hit
GATTCAGGGATTGTTAATTGTGTTTAAACAAAGGCAAAGATTACTGACTAAT2630.12392157601858352No Hit
GGGGCAGGGGTACAAGTGTTTCTAGTAAGTGGCATGCCACTTTGTATTGCTT2600.12250802191951222No Hit
ACCTTAGGGATGTATCACTAACTCATATAGAATTCTTGTTTCTTATTCCCAG2600.12250802191951222No Hit
AACGTGGGATCAAGTGAAAATTAAATTCTTTTGGGAAACATTTTCCTTCTGA2590.12203683721982178No Hit
TTTTAGGGGTACCTCACCTTCCTGGGGAAACCTTCATAGACGCCTCACTGTT2590.12203683721982178No Hit
GCATGTGGGGACGGCAATCTCATCTAGGCCACAAGAGAAACCTGATAAAAAT2590.12203683721982178No Hit
ACCACTGGGAGCAGCCTTTTACCTCTGTGCTTTGTATGCTTTGTGTGTCTGG2580.12156565252013138No Hit
TGCACTGGGGATAATTAATAATTGGAGCTGCAAAGCAGATCGTGACAAGAGA2570.12109446782044093No Hit
ACACAGGGGAGGAGACATGAATTAAGAAATTGGGAACAAATTAAAATAACAT2570.12109446782044093No Hit
ACGCAGGGACTTGCTGCTTACTCTGGCATGTCAATTATTGTGCCAGCTGGAA2540.11968091372136964No Hit
ATACAAGGGTAAGCTATCAGATGAACTTTGCTTTTTTATGGCAGACAGGTCT2520.11873854432198877No Hit
TGAACTGGGTATTTTTATTCTGTCATCTTGTGGTTTGGCCAAGATTGATCAG2500.11779617492260791No Hit
TAGGTGTGGGCTTTTCGGCTGGAATGGGGAATTTGTGAGGTAGGCCGGAAGT2470.11638262082353662No Hit
GTGGTTGGGGCTACTACGATGGCGATGAGTTTCGAGTGGCCGTGGCAGTATC2460.11591143612384618No Hit
GCTGCTGGCTATTTCTGCAAAGCCACTTAACTTTTCTTCCTGATTCCATTTT2460.11591143612384618No Hit
TCAAAAGGGGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAGGAGGTT2450.11544025142415575No Hit
CTGATAGAGGGATAACTCAGTATTTTAAACCGATAACACTGATTGCATAAAC2450.11544025142415575No Hit
GCATATGGGAGACTACTGAAGGACTTGAGGATTCGTTTTCCTGGCTTTGAGC2440.11496906672446533No Hit
CGCATGGGGTGTCAGATAATGCTACTTTATCCTCTCATGGTTAACTAAAGTG2440.11496906672446533No Hit
GCAAAAGGGGGGAGCCTTCCCGGATATTTAAGCTAGAAGATTCTACAGGGAG2440.11496906672446533No Hit
GTTAAAGGGATTGCTGCTTTCTCTTCTACTTCTTGTGCTTTACTCTGAATTT2440.11496906672446533No Hit
TAGGCCGGACGGCGTGTGTTGTGAAACGTGAAATAAAAAGAGCAAGAAAGAA2430.11449788202477489No Hit
GCTGGCGGGGTACTGTGCTGGGGCACATATCTTGTTTTCCCAGCTGCTAAAC2410.11355551262539404No Hit
TATTGGGGGGGTTCTGTCTCCAGTTTTGTGCCAAGGAGAGAATGGTGTCCCT2410.11355551262539404No Hit
TCGGGGGAGGCCACGAAACTAGAAAAGTTCTTCATTATAACTATGTATAATT2410.11355551262539404No Hit
CACTATGGGATGTGTTTGGCTGGATAAATTCCAAATTTGTAACTAGAAGCAA2400.1130843279257036No Hit
ACTGAAGGGGCAGTCAAGAAAAATGACAACAGATGATGGCGTCTTTTCAGTC2400.1130843279257036No Hit
CACGCCCGGGCTCTGTGGTTAGCGGGATGTGCTTTTTTTTCTTTTTTCTTTT2380.11214195852632275No Hit
AACAGCGGGAGAGCATTTCGGGGGGGGTGGGGGAAACACACCTAAACACTTT2380.11214195852632275No Hit
TCTCTTGGGATATTATGAATTGCTTAGCAATGAAATGCAAGTATGCATCTTT2370.1116707738266323No Hit
GTAAACGGGGATCCAGCCCCCTTATAAGCCAAAAGCTTGTGGGCGAAATGCT2370.1116707738266323No Hit
AGCAATGGGGAAGATGCCAAAGAGCATGAAAAAGAAAAATCAGTTTCATCTG2360.11119958912694186No Hit
GAATTTGGGGAAAGTTTTCAAAGAACTCTATCCTATGCAGGGGCCCAGGATT2350.11072840442725143No Hit
TGGATGAGGGAGGAGGTGATGTCCAAGCCTAATTTTTACTCCAGCTATGAAA2350.11072840442725143No Hit
CTGTTAGGGAAGACTGAATCACTGAAAAGTGTAAGTCTACCTCTGAAGGAGC2330.10978603502787057No Hit
GCTCGCGGGATTGCCACTCCTCACGATGGCCTATTTAAAGGGCGACCTCCGG2330.10978603502787057No Hit
TACAGTGGGCATATCGTCAGTCCAGGAGCAGCTGAATCTAAGGATGCAGACT2320.10931485032818014No Hit
TTTGGTGGGAGGCGGGAGGATCACTTGAGCCAGGCCTAATCCTGAGCTTTTA2320.10931485032818014No Hit
ACAATTGGGACTGACAGCTGAGACCTCCTACTCAAGTGCCTGTTGCCTTCAG2320.10931485032818014No Hit
GAACTAGGGCTGTGTGGTAACACCTCAGTTGTGTTCTCCTGACACCAGGAAA2310.10884366562848972No Hit
GTGGGTGGGATCATTATTTTACACTAACATGGTGAGTGTTTTAGATTATTTT2310.10884366562848972No Hit
GCTTAAGGGAATATCAGTCCTATGAGTCACTTTTCTAGCCATAATTTTAACT2310.10884366562848972No Hit
AAAAGTGGGCGATCTTCGGTTGGGCAAATAGATGAGTCGTAGTCTCAACAAT2310.10884366562848972No Hit
CGTTATGGGAAGAGTGATGCAAATTCTTTTCCTGCTTTCCGTTTCTGGTTTC2300.10837248092879928No Hit
TACGACGGGTGAGATGAAAAATGAGATGAAATGATGAATTGAGATGAGATGA2290.10790129622910885No Hit
GCTAAGGGGGGCCAGACTGTGCCAAAAAACGTTTTGCAAACCACGGGTTCAA2290.10790129622910885No Hit
AATTCGGGGGTCAAAACCCATCATAAGATCACTGTCAGAATTACTGGGGATC2280.1074301115294184No Hit
ATGAGCGGGATTGAGTGAAAATATCACAGTGTTTGAGATACAGCTAAAACTG2280.1074301115294184No Hit
TGTGCAGGGGACATGAATCCGAATTCTACACTTCCAAATCAAATAGGTAAAA2280.1074301115294184No Hit
TTCTTAGGGTCCTTGCAGAGGCCATGGAGGACGAGGAGAGACAGAAGAAGCT2260.10648774213003756No Hit
CATCTCGGGCATACAGAAGATAATCAAATGTTGATGGACTCTGTGCTATGAG2260.10648774213003756No Hit
CAAATAGGGACATGGACCAAATGCATCAGAATTTTGAAGAACAAATGCAAGG2250.10601655743034712No Hit
ATACTTGGGGCTGGCTTAACCATCTCTTTGTACAATCTGTTCTCTCTGTTCT2250.10601655743034712No Hit
TTACCAGGGGGACCAGTCCCTCGGGGGGAACGTGGTCATCAGTGGTCAGTGG2250.10601655743034712No Hit
CAACGAGGGTTATTTGTTAGATGAGTAAGCAAGATACCATTGTCGTCATTGG2250.10601655743034712No Hit
TCATGAGGGACTTGTTGAAATGAATACAGGCGAAGCTGAGTCAAGAAATTCA2240.1055453727306567No Hit
CGCATTGGGGAAAAATAAAGTAGCCAATTCTAAAAGTAGCCCTCTTTCTCCT2240.1055453727306567No Hit
GTCGATGGGTTGTGGAGAATTGAAATTATAATAAGATTGATTCTTCCTGTCT2240.1055453727306567No Hit
AGCGTGGGCATACATCAACATGAAGGTGTGACATCAGAATCATGAGCACCAT2240.1055453727306567No Hit
TAAAATGGGGATTAGTTGAACTGGCATCCTGCTTTAGCCTGAGACTTGCCAT2240.1055453727306567No Hit
TGCAAGGGAATGCTTTGAAAAGAATGAGGCCATACAGGCAGCCCATGATGCC2240.1055453727306567No Hit
CTTGCCGGGCGATCTTTGGTAGGGTAGCACCAAGAATTATAGCGTTTATAAG2240.1055453727306567No Hit
CTTACCGGGCTTCCGATCTACTTTAGGGTATATGTAGGATAAACATAGGTTA2230.10507418803096626No Hit
TAGCTTCGGGGGCAGCCGGAGTAAGATGGCGGCGCTGAGGGCTTTGTGCGGC2220.10460300333127583No Hit
TTAGAGAGGGAATCCAAGAATTCTAAGGGAGGTACTAGCAAATAGTATCCAG2220.10460300333127583No Hit
GTGCAAGGGGGTTCTCTCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCAT2220.10460300333127583No Hit
GCTGCTGGGGTTGGTTACAAAACAAATTAAATCTCTATTGTTAACTTTTAAG2220.10460300333127583No Hit
GTTCTGGGGATTATTCCCATTTTAAACACAAAACTCTTAAAGCTCTAAGCAT2220.10460300333127583No Hit
CTCAGTGGGGGGCTTGGCGGTTGGAGCGGCTGTCGCCATGTTGTCGGTAGCA2220.10460300333127583No Hit
GCTCAGGGGGAAGGGCAGCTCAGAAGAATGCAGGGCCCATTCAGCATGGGGA2210.10413181863158538No Hit
CAGCGGGATCTTTCCTTTTAGTTTTGACAGAGATTTTCTATTTTATCATTAT2210.10413181863158538No Hit
ACTGCTGGGATTTTGCATGAATCCCAAATACAGCACCATTTTATTACCTATA2210.10413181863158538No Hit
CAGCGCAGGGAGAAGCTGCAGTTACTAGAAGAAAAAACAGATGAAGATGAAA2200.10366063393189497No Hit
ACACAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2200.10366063393189497No Hit
TACAGGTGGGAAGCAGTTTCCTAGAGGCAGCATGATACAAATTCTGGAGCAA2190.10318944923220454No Hit
TGACATGGGAGCTGACCACTGGCTGAAACCTCATGAGAGATCCCAAGGTTGG2190.10318944923220454No Hit
AAACAAGGGGAAAAAACTAAGGACCTCATAGTTGACCAGACGATAGAGAAAG2190.10318944923220454No Hit
AATAGTGGGCCGACCCCTACTTCTGTTTCTTCCTAAGGTATAATCTGCAGAT2190.10318944923220454No Hit
CACCACTGCCTCCTGTCTTTCCACCAAACTTAACAATCTTTTGATCTCTATT2190.10318944923220454No Hit
AGATGCGGGGGATCAGGGAGGTCACAGTGCAGTGTAGAGTGGCCGCTTCTCC2180.10271826453251409No Hit
TCACGTGGGAACAAAAGGAACTTTATAGTACGAAACGGTGGCAAACAGTACC2180.10271826453251409No Hit
TAGTCGAGGGATGAGACGGCTGCTGTGCGTACTCCAAACTAGCTATGCTCCT2180.10271826453251409No Hit
ACAACAGGGTCCAGACCTCAGAGTAAACCATAGCTTTTTATTTTAACTGGTT2180.10271826453251409No Hit
ATACTAGGGGAGGCTAAGAAACTTTACCATCCATACACATCTAGAAAATGGC2170.10224707983282368No Hit
CATATAGGGATTGTGGTCACTGATCTCTCATATAAAAGTGAGGGTGGCGGGG2170.10224707983282368No Hit
ACCCCTTGGTGCAAACAACTCCTATTATTTCTACCTGTTTCTCAAACATACT2170.10224707983282368No Hit
TTACTAGGGACATGAAACTCACCCTTGAAAAACAATTCAGTAGTTTTTAGTA2170.10224707983282368No Hit
AAGCAAGGGGCTAATACCTATTCTTCAACCACCTCGGTTACTCTGACATAGG2170.10224707983282368No Hit
ACCGTCGGGCTAAAAATGTTGAAGGTACCTGACTATATTACTACCTGAAAGA2170.10224707983282368No Hit
GCTTTCGGGCAGAACATTAAACTTAGTTTTCATAGAAATTGCAATTCTCTTT2160.10177589513313325No Hit
CAGGCAGGGGGGAGATTAGAGGATGATTTGATAGATTTGGGTAAAGCTTTTG2160.10177589513313325No Hit
CATTATGGGGAGAAGGAGAAGGAGGAGGACTAGGAGGAGGAGGACGGCGACG2150.1013047104334428No Hit
TGCCGTGGGAGAAAAGTGAATAATTCAGTCATTCATCTGGATGTAGTTTTCT2150.1013047104334428No Hit
TGAGTGGGGCATATCGGGATGGGCATTGGACCTGCCTATGCCATCCCAGTAG2150.1013047104334428No Hit
TGATACGGGCCTCAGACACACTCTGATAAATAGACATACCTGACAATGTACA2150.1013047104334428No Hit
GTATACGGGTATGATCTTGGTTCACTGCAACCTCCACCTCCTGGGTTGAAGC2140.10083352573375237No Hit
GTGTGGGGGGAGGTCACTCAGAAGGGAAAGAGAAGACTAAGGGATGCCTTTG2140.10083352573375237No Hit
TGGGATGGCAAATCACTTGAACCTTTTTGAGTTTTACTTTACTAATCTCTAA2140.10083352573375237No Hit
TGTACAGGGTGGCCAGGAAATTATTTTGCATTTTAATGATCACCAAATTGAT2140.10083352573375237No Hit
GAGTACATATTGTGGTGATGGAGATGCAGTCACTTATTGTCCAGGTGAGGCC2140.10083352573375237No Hit
CGAATGGGGTTTTCTAAATATACAATCATGTCATCTGCAAACAGGGACAGTT2140.10083352573375237No Hit
CGCTTCGGGGTATTCCTGTGCACTACTGTTACGTGAACACACAAACACCTAA2130.10036234103406194No Hit
TTGCTGGGGTTACAGGCGTGAGCCACCATGCCCAGCCCACAGTAGGACTTCA2130.10036234103406194No Hit
CTCTAGGGCGGAAGTTGTTTTACCTACTTTAAAAGTTTTAATGGATTGCACC2130.10036234103406194No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)