FastQCFastQC Report
Sat 31 Dec 2022
SRR5845553.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845553.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences208268
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCATGCACATCTCGTATGCCGTCT6540.3140184761941345No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCATGCACATCTCGTATGC5710.2741659784508422No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG3860.1853381220350702No Hit
CAGTTAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA3360.16133059327405075No Hit
GACACCGGGAAGAAAACACATTAAAAACACAGGTAAAAAGTAAAAAATAGAT3170.15220773234486334No Hit
AGTGCAGGGCCATTTGGGAATTCAATACAGGAAACCAAGAAAGAGAAACCAT3040.14596577486699827No Hit
GCATGTGGGGACGGCAATCTCATCTAGGCCACAAGAGAAACCTGATAAAAAT2910.13972381738913325No Hit
TAAACTGGGATTTGAACATATTTCTTCCATCATGTATTTTCTGCTCATCACC2910.13972381738913325No Hit
TGCTGGGATTACAGGCATGAGCCACCTTGCACTGCCTGCCTCATATTATTTT2790.13396201048648856No Hit
GCACTTGGGAACCCAAGTCTTGAGCAATGTTTTTCTCAAAAAGCTGCTATCC2760.13252155876082738No Hit
TAGGTGTGGGCTTTTCGGCTGGAATGGGGAATTTGTGAGGTAGGCCGGAAGT2730.13108110703516623No Hit
TATGGTGGGAAGCTTCATAAGTGAAGGAGAAATAAAATTCTTTACAGACAAG2680.1286803541590643No Hit
CTTTCTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2680.1286803541590643No Hit
AATTCGGGGGTCAAAACCCATCATAAGATCACTGTCAGAATTACTGGGGATC2610.12531930013252157No Hit
GTCATGGGGGTGTTAGGCAACAAAAAGTAGTAGAAATAAGAAAAATGTAATC2610.12531930013252157No Hit
TCAAAAGGGGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAAGAAGGAGGTT2610.12531930013252157No Hit
GCTTAAGGGAATATCAGTCCTATGAGTCACTTTTCTAGCCATAATTTTAACT2600.12483914955730116No Hit
TTGTTAGGGATGGCTCAGAAACATAACTGTCTTTGGAATCTAGTACAAGCTC2580.12387884840686039No Hit
TACAGTGGGCATATCGTCAGTCCAGGAGCAGCTGAATCTAAGGATGCAGACT2580.12387884840686039No Hit
TTTTAGGGGTACCTCACCTTCCTGGGGAAACCTTCATAGACGCCTCACTGTT2570.12339869783164No Hit
ACTCGCGGGGGCTTCTCTGGGAGCTCTCCAGTGGCACTGCTGGACCTGCCCA2560.12291854725641961No Hit
TATTGGGGGGGTTCTGTCTCCAGTTTTGTGCCAAGGAGAGAATGGTGTCCCT2530.12147809553075845No Hit
TAGGCCGGACGGCGTGTGTTGTGAAACGTGAAATAAAAAGAGCAAGAAAGAA2510.12051779438031766No Hit
TCGGGGGAGGCCACGAAACTAGAAAAGTTCTTCATTATAACTATGTATAATT2510.12051779438031766No Hit
TAGCTTCGGGGGCAGCCGGAGTAAGATGGCGGCGCTGAGGGCTTTGTGCGGC2480.1190773426546565No Hit
AGTAGCAGGAAGATACAAGAGAACAAAAAGATAGTAAAATAAGCATTCTCTA2460.11811704150421572No Hit
TGTGCAGGGGACATGAATCCGAATTCTACACTTCCAAATCAAATAGGTAAAA2450.11763689092899533No Hit
TTACCAGGGGGACCAGTCCCTCGGGGGGAACGTGGTCATCAGTGGTCAGTGG2430.11667658977855455No Hit
CGTTATGGGAAGAGTGATGCAAATTCTTTTCCTGCTTTCCGTTTCTGGTTTC2410.11571628862811377No Hit
AAAAGTGGGCGATCTTCGGTTGGGCAAATAGATGAGTCGTAGTCTCAACAAT2410.11571628862811377No Hit
TGGATGAGGGAGGAGGTGATGTCCAAGCCTAATTTTTACTCCAGCTATGAAA2400.11523613805289339No Hit
ACACAGGGGAGGAGACATGAATTAAGAAATTGGGAACAAATTAAAATAACAT2400.11523613805289339No Hit
TTCGTTGGGGGCCATAAAGATAATGCTGCATTTTTCTGTCAGAATCACATAT2390.114755987477673No Hit
CTCTTTGCGGGATGATTCATTCAACCAAGTCGCTGGACTTTTGAAGGCCTAT2380.1142758369024526No Hit
ACGCAGGGACTTGCTGCTTACTCTGGCATGTCAATTATTGTGCCAGCTGGAA2380.1142758369024526No Hit
GTTAAAGGGATTGCTGCTTTCTCTTCTACTTCTTGTGCTTTACTCTGAATTT2360.11331553575201184No Hit
AACGTGGGATCAAGTGAAAATTAAATTCTTTTGGGAAACATTTTCCTTCTGA2360.11331553575201184No Hit
TCTCTAGGGCCTTCGGCAACCTGAGCGACTCCCGCCTTCCACGGAAGGGACC2350.11283538517679144No Hit
CATCTTGGGGGTCTTTTGTAATAATGTTGGGGCACTTTAAACCTCAGAAGCA2340.11235523460157106No Hit
CACTATGGGATGTGTTTGGCTGGATAAATTCCAAATTTGTAACTAGAAGCAA2330.11187508402635067No Hit
AATGAGGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACA2330.11187508402635067No Hit
TAATTTGGGTTGACTTCGCTCTTATCAAATGTTTTCCTATTTCTATTAATTT2330.11187508402635067No Hit
CAACGAGGGTTATTTGTTAGATGAGTAAGCAAGATACCATTGTCGTCATTGG2330.11187508402635067No Hit
GATTCAGGGATTGTTAATTGTGTTTAAACAAAGGCAAAGATTACTGACTAAT2320.11139493345113027No Hit
TGCAAGGGAATGCTTTGAAAAGAATGAGGCCATACAGGCAGCCCATGATGCC2310.11091478287590989No Hit
ACAACAGGGTCCAGACCTCAGAGTAAACCATAGCTTTTTATTTTAACTGGTT2310.11091478287590989No Hit
ATGTAAGGGGGGAACAAAACGAACTTTTTTCCTGGTTGAGCATTAACTGACA2300.11043463230068949No Hit
GAATAAGGGATACATAATATTTGGTAGCTTGTAAATGAAATAAAGAATAAAG2300.11043463230068949No Hit
TTACTAGGGACATGAAACTCACCCTTGAAAAACAATTCAGTAGTTTTTAGTA2290.10995448172546911No Hit
ACTTTGGGGGGGATCAGATGTTCACCTAAGGCCTGGAGTTAATCTGGGGCCT2290.10995448172546911No Hit
TGTACAGGGTGGCCAGGAAATTATTTTGCATTTTAATGATCACCAAATTGAT2270.10899418057502833No Hit
TGAACTGGGTATTTTTATTCTGTCATCTTGTGGTTTGGCCAAGATTGATCAG2270.10899418057502833No Hit
TACGACGGGTGAGATGAAAAATGAGATGAAATGATGAATTGAGATGAGATGA2270.10899418057502833No Hit
GCTATGGGGTTTTGAGTGTTTTTTGAGCCCCAGACAAATAATGTTTTAAAGT2270.10899418057502833No Hit
TTCTTAGGGTCCTTGCAGAGGCCATGGAGGACGAGGAGAGACAGAAGAAGCT2260.10851402999980794No Hit
GCAAAAGGGGGGAGCCTTCCCGGATATTTAAGCTAGAAGATTCTACAGGGAG2260.10851402999980794No Hit
GTCGATGGGTTGTGGAGAATTGAAATTATAATAAGATTGATTCTTCCTGTCT2260.10851402999980794No Hit
GTGTCAGGGAGAGCAGGGACTGTTTTGAATTTACTACCTATTTGGGCCTTGG2260.10851402999980794No Hit
TATAGCCGGGCTTTTTTAAAATTGTGTTCTTAAAATATTGTGTAAGAATCAC2240.10755372884936716No Hit
GTTTCTGGGCGATCTTAGGCGGGGGTCTTGGGAAATTTTATTATCTTCTGTC2240.10755372884936716No Hit
ACGATAGGGGGTTTTCATTTCCTGGAAAATATTGTCTTTGTGGTCATTCAAC2240.10755372884936716No Hit
GTGCAAGGGGGTTCTCTCTTTCCGGACCTGGCCGAGCAGGAGGCGCCATCAT2230.10707357827414678No Hit
AACAGCGGGAGAGCATTTCGGGGGGGGTGGGGGAAACACACCTAAACACTTT2230.10707357827414678No Hit
GCTGCTGGCTATTTCTGCAAAGCCACTTAACTTTTCTTCCTGATTCCATTTT2230.10707357827414678No Hit
AGCAATGGGGAAGATGCCAAAGAGCATGAAAAAGAAAAATCAGTTTCATCTG2230.10707357827414678No Hit
TAAAATGGGGATTAGTTGAACTGGCATCCTGCTTTAGCCTGAGACTTGCCAT2220.1065934276989264No Hit
CGTTCTGGGAAATGAGTGTTATCAAAAAGAAACAAGAGAACAAGTGTTGGTG2220.1065934276989264No Hit
GCATATGGGAGACTACTGAAGGACTTGAGGATTCGTTTTCCTGGCTTTGAGC2210.106113277123706No Hit
GTAAACGGGGATCCAGCCCCCTTATAAGCCAAAAGCTTGTGGGCGAAATGCT2210.106113277123706No Hit
CATTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2210.106113277123706No Hit
TTGCTGGGGTTACAGGCGTGAGCCACCATGCCCAGCCCACAGTAGGACTTCA2210.106113277123706No Hit
CACCACTGCCTCCTGTCTTTCCACCAAACTTAACAATCTTTTGATCTCTATT2210.106113277123706No Hit
GGGGCAGGGCAATAAAACTAATAAAAATGAATACTGTATGGGACTAGAAGTA2210.106113277123706No Hit
TGCACTGGGGATAATTAATAATTGGAGCTGCAAAGCAGATCGTGACAAGAGA2200.10563312654848561No Hit
CACGGTGGGGGCTACAAGTCTGTTTTGTACATGTTACAGTTTTCGGTAATTA2200.10563312654848561No Hit
CACGCCCGGGCTCTGTGGTTAGCGGGATGTGCTTTTTTTTCTTTTTTCTTTT2190.1051529759732652No Hit
AAGTTCGGGAGTTGCAGCTGATGCCTGAATGTATCCTAGCTGACAAATTATT2190.1051529759732652No Hit
AAATTTGGGGAGTGCGACAGACAGCCTCAGAGCTGGTGGGGAGACAGCATGC2190.1051529759732652No Hit
GCTAAGGGGGGCCAGACTGTGCCAAAAAACGTTTTGCAAACCACGGGTTCAA2190.1051529759732652No Hit
GCCAACGGGAGGGACTGTTTGTCAGTATTGCTGTCATCCCCAAAAGAAATGA2190.1051529759732652No Hit
ATACAAGGGTAAGCTATCAGATGAACTTTGCTTTTTTATGGCAGACAGGTCT2180.10467282539804483No Hit
ACCGTCGGGCTAAAAATGTTGAAGGTACCTGACTATATTACTACCTGAAAGA2180.10467282539804483No Hit
TCATACGGGTATTCAATTTTTCACTTTATTAGTAATGAGATGTAAGCTTTAA2170.10419267482282445No Hit
TAGCCTGGGCTGCAGAGAGAGACTCTGTCTCAAACAAAAAAGAAAAAGAAAA2160.10371252424760403No Hit
TTTGGTGGGAGGCGGGAGGATCACTTGAGCCAGGCCTAATCCTGAGCTTTTA2160.10371252424760403No Hit
ACATATGGGTATTGCTTTGGAGTGTAATATTTTTAATGTATTCACTATTTCA2160.10371252424760403No Hit
AATAGTGGGCCGACCCCTACTTCTGTTTCTTCCTAAGGTATAATCTGCAGAT2160.10371252424760403No Hit
GTTAATGGGCAAAGGAAGCACTTTATTTTCTACAATCTATTGCATTTTCCAA2160.10371252424760403No Hit
CGCGACAGGGTTTTGGAAGTATTTTATGAACTAAAAGTTAGCAAGCCCCCAT2160.10371252424760403No Hit
TGAGGGGAATGAGAAAAGAAAAAAGTCATGGTAGGTGAGGTGGTTAAAAAAA2150.10323237367238366No Hit
CATAATGGGAGGCAGGCCAATATTCAAATTCAGGAAATACAAAGAACACCAC2140.10275222309716328No Hit
CAAATAGGGACATGGACCAAATGCATCAGAATTTTGAAGAACAAATGCAAGG2130.10227207252194287No Hit
GGGGCAGGGGTACAAGTGTTTCTAGTAAGTGGCATGCCACTTTGTATTGCTT2130.10227207252194287No Hit
CAGTCAGGGACTCGGTTTCTGCTCAGAGCTCGATGTCAGGCATCAGATGACT2130.10227207252194287No Hit
CGAATAGGGGAAATTAAAGGCAGAAAAACTGGAAAACCTACTGCAGGTCCAG2130.10227207252194287No Hit
GTACGCGGGTTTTCTAACTTTGTTTTGTCTTATTTTACCTAAGAACACTGGA2120.1017919219467225No Hit
TGCCGTGGGAGAAAAGTGAATAATTCAGTCATTCATCTGGATGTAGTTTTCT2120.1017919219467225No Hit
ACACAGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2120.1017919219467225No Hit
TACTCAGGGGACTATCTCAAACTCCTTGGCAAGGGAACCTTTGGCAAAGTCA2120.1017919219467225No Hit
GAATTTGGGGAAAGTTTTCAAAGAACTCTATCCTATGCAGGGGCCCAGGATT2110.10131177137150212No Hit
ACCATTGGGATCACATATATCTGTATATCTTGGGCAAGTTACTTAACATATC2110.10131177137150212No Hit
TATCTAGGGAAGAGTCAAGATTGGGCAGCTGCATCTGATGAAGGCCTCATGT2110.10131177137150212No Hit
GCTTTCGGGCAGAACATTAAACTTAGTTTTCATAGAAATTGCAATTCTCTTT2100.1008316207962817No Hit
TATGCCGGGTACTGGGCCTGCGCTCCTTTGCAGAGATTGGCAGAGGGAAAAA2100.1008316207962817No Hit
CGCATTGGGGAAAAATAAAGTAGCCAATTCTAAAAGTAGCCCTCTTTCTCCT2100.1008316207962817No Hit
TGACATGGGAGCTGACCACTGGCTGAAACCTCATGAGAGATCCCAAGGTTGG2100.1008316207962817No Hit
ACCACTGGGAGCAGCCTTTTACCTCTGTGCTTTGTATGCTTTGTGTGTCTGG2090.10035147022106133No Hit
TCAGCTGGAGAGAGAGAGACTGATTGACAGCTTTGGGTGCATTATCACTGGT2090.10035147022106133No Hit
TCACCAGATACCGCGCCTTAGAACAGAATAGATGCTTAGCAAACCATTACCA2090.10035147022106133No Hit
ATAACTGGGTTATGGTTCTGGCAGCTACTAATTTTCCCTGGGATATAGATGA2090.10035147022106133No Hit
ACTAGGGGGCTGGTTGAGGGTAAGCCCAATGCTTTCATCAGAAAAACCACTC2090.10035147022106133No Hit
TATCTGGGGGGAAGTAAAGCTTTGGAGGAAAATCAATATCAATGAAAGTGCT2090.10035147022106133No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)