FastQCFastQC Report
Sat 31 Dec 2022
SRR5845551.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845551.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences86111
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCATGCACATCTCGTATGCCGTCT5200.6038717469312863No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCATGCACATCTCGTATGC4470.519097443996702No Hit
GCTTATGGGACGCCCTTGTAATCCTAGCTACTTGGGAGTCTGAGGCGGGAGA1560.1811615240793859No Hit
TCCGCCGGGGATATCTGTCTTTCCGTGTCTGGCTTAGTTCACTAAAAAAATG1410.16374214676406035No Hit
GCAAAAGGGCACGCCACCCGCGTCATCGCCCTTATCAACGACCAGCGCCGGG1370.1590969794799735No Hit
CATTAAGGGAAAGAATAAGTATTGAAATGAAAGAATGACAGAAAGAATGAAT1280.1486453530907782No Hit
TAGTCTGGGCACGAACACGTTTTTATTTTTCTCTTTTTATGAAGGCTTGCTC1270.1474840612697565No Hit
ATATCCGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1240.14400018580669136No Hit
GATGCTGGGGAATCAATTCAACAAGAAGAGCTAACTATCCTAAATATGTATG1190.13819372670158284No Hit
GTCATGGGGATAAAAGGAAACCTTTTGTTGCTCCCAAGAAGAAAAGAAAAAA1170.13587114305953943No Hit
GACTTTGGGGTCAGCTCACTGCAAGCTCTTCCTCCCGGGTTCACGCCATTCT1140.13238726759647432No Hit
ATCGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1120.1300646839544309No Hit
ATAACTGGGAGCAGCCAAGATGTTCAGTAAAGCCTTTGACAGTGGCATTATC1110.1289033921334092No Hit
TTATGGGGCATTATTAAAAAAAGAAACATCTCAGCCAACAAGGAATATAAGG1090.1265808084913658No Hit
CTAGGTGGGATAATAGGCTTGAAAATTGATATCCTGTGGTGCTAAAGTACAG1090.1265808084913658No Hit
GGGAAGGGGTGTCTGCAATGAGCTAGAGAGCTAGTTTTTTTTTAAATAAGGA1090.1265808084913658No Hit
CGCCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1080.1254195166703441No Hit
TATAGAGGGCTCAGTGGGAAACTTTATTTGGTTGATTTGGCTGGGAGCGAAA1080.1254195166703441No Hit
CTAGCTGGGAGAAAAGAGATATGTTTTCTGGAGTCATAAAGGAATTCAATTC1050.12193564120727897No Hit
ACGAAGGGAGACTGTGGGTGGATTTTCGAGCTGACGGTGGTCAATTCCTTTC1040.12077434938625727No Hit
GCCTAGGGGACAATTGACTTCCCTGAATTTCTGACAATGATGGCAAGAAAAA1040.12077434938625727No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1030.11961305756523558No Hit
GTATTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1020.11845176574421387No Hit
ACTGGCGGGCAGACAGTGCTTGAGCTGCAGAAACTGAGACCAGACCTCTGGC1010.11729047392319215No Hit
GTTCATGGGCATAAAAGTCCTGTCACCAAGGACGTTAAAGGCATTTTATTCC1010.11729047392319215No Hit
TTAGTGGGGCCTAGGTTTCGAATTTCTGTCTTGTTACGATTTTTCTAACTTG1000.11612918210217045No Hit
TGCACTGGGTCCTGGAAGTTTTTATTGTTGTCAAATCTGGCCTCTCTGGGAA1000.11612918210217045No Hit
TTTATAGGGCAACACTCCACTTTCTGCATTGGGCAGATCTTGAAGACAGAAA990.11496789028114876No Hit
CTCCCTGCCCTCCCCCACTCCATTCAGTTGATTCATTTATGCAAATTCTGTT970.11264530663910534No Hit
GATTTGGGGTGACGGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGT970.11264530663910534No Hit
GCATGCAGTTTGGAAATAAAATTTAAACTCATTTATGTGTAATAAATATGTA960.11148401481808362No Hit
TCCAAAGGGATAAAGACATAGTAGACCAACGGAATAGAATCTCTAGCCAAAA960.11148401481808362No Hit
CTCAAAGGGGAATGTCCGGTCAAAAAATAAGCGTTCCTTCCGAGAAAAACAG960.11148401481808362No Hit
AAGCGGGTAGAAGTCCTAGCAGAGCAAATAGACAAGAAAAAGAAATTAAAAA950.11032272299706194No Hit
TTTCCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC950.11032272299706194No Hit
CTGTGTGGGGTGATTCAGTTCAGTAATGGTGATTCAGTTCAGTAACTGCCTA950.11032272299706194No Hit
GAGACAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC950.11032272299706194No Hit
TCGCTCGGGCTACTTCTAAAAATTATTTTGAAAACGAAAGGAATATGTTTGG950.11032272299706194No Hit
CGTACAGGGCAACAACATCCAAGCTGAGAGCCAAATCAAGAATGCAATCCCA940.10916143117604023No Hit
CAAATAGGGGGAGCCACTGTGCACTTTGCCCAGCTGGCACGCTCACAGCTAC940.10916143117604023No Hit
CCAAAAGGGGAAGCCTGAAAAGGAGGAGCAACCTGTCCAGAATCCCCGCAGG940.10916143117604023No Hit
AGTGCAGGGGCACCCACAACAATCAGTGTTAATAATCCCAGAACAGTTAGCC930.10800013935501852No Hit
ATGCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA930.10800013935501852No Hit
CGTAAAGGGCAGCCACTTGCAACACAATGTTTCCAACTCTCTAACATGTTTA930.10800013935501852No Hit
ACACGAGGGTGGGAGAGGGGTGTGATGAGAGATTAGGGGCAATGTATGTTAT930.10800013935501852No Hit
ATTTTAGGGCATAGGAGGAGGAAGAAAAGCTAAAAAACAAACAAACAAATAA930.10800013935501852No Hit
GCTTTGGGGCAACTTGGTCAATCTACATGGTGTGACAACAGATATAAGGAAA930.10800013935501852No Hit
CAAAATGGGGATGTACCTTTAAGCAACTTTTTAAGCCTCAAGGCACTTATCT910.10567755571297512No Hit
TCTTAAGGGATCGTAAAACATGAGCCTTTCCAGAGTCAGCTTAGACACTGTT910.10567755571297512No Hit
CGAATTGGGATTCAAATCTCTTATGCCAACAGAAAAGTTCCTTCTTGTTTAA910.10567755571297512No Hit
TTGCCTGGGTCTCTGGTGTCACCCAGATAGATTGTGCTTACTGAGACAAATG900.10451626389195341No Hit
TTCGTAGGGGGTGAAAGAAATCCTGTCAAATTAGTTCCAGGAAGGGTTTGCT900.10451626389195341No Hit
ACACAGGGCTCTTTCCCTAAGCAGCCTGAGGTGATCTGTGAAAATGGTTCGC900.10451626389195341No Hit
GGTAGTGGGACCCTCAATAAAGGCTGTTGCCTAAGCATGGCATACTTCATCT900.10451626389195341No Hit
ATTCTTGGGATGACACTGACACTGCTATTTTGAAACCTGGAGGATGGAAAGG900.10451626389195341No Hit
AACATGGGGATATTTAGACAGGATTTCCAAAAACTTTGTCTTCAAATTTTAA900.10451626389195341No Hit
ACCACTGGGATAAGAAATATATTTTACAACATGACCCAAATACACACATCAG890.10335497207093171No Hit
TAACACGGGATGCCTTACTGATACATTTGCATTTTGTCTACTAGTTACGGTT890.10335497207093171No Hit
ATGCGAGGGTGCCTCTAAACCACTTTTATTTGTGTGGTTTTAGACGAATTGA890.10335497207093171No Hit
AAGACAGGGCAATTGTTGCCATTGAAAACCCTGCTGATGTCAGTGTTATATC890.10335497207093171No Hit
GCTGCAGGGATGAATTCTTTAGTTCTGACATATTATTTTCTATTTTTAGCAT890.10335497207093171No Hit
GGTATAGGGACAATAAAAAGTTGAGAATCAGGTGAAATTAAGCTTATTAATA890.10335497207093171No Hit
CTCGAAGGGATGACATTAATCTTTAGTCAAAACCCATTGAGGTGTCTGACAG880.10219368024990999No Hit
GATAACGGGTAGGTCACATCTGTACCTAATGAAGAGAACTGCACATTTTCTG870.1010323884288883No Hit
AATAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC870.1010323884288883No Hit
GCTCCAGGGCGTTAAGAGCAAGGAGGACCCATTGGGGGATTCATAGAGGAAC870.1010323884288883No Hit
TGCGTAGGGACCCTCCTTGCATAAAATATCACACTTGGGTAAGTGCAGTATG870.1010323884288883No Hit
GCTATAGGGAGGAGTCTGTGTTTCTGTGTCATAATATTTATTGCATTAAATT870.1010323884288883No Hit
CAAAGCGGGGAGCACAGGGAACAGGCCGCCACAGAAAAGGTTGCCTTTTATG870.1010323884288883No Hit
ATTAGTGGGGTGATATAACTAAAGGTAAACACAGTTATAATAGCAGAACAAG870.1010323884288883No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)