FastQCFastQC Report
Sat 31 Dec 2022
SRR5845550.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845550.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85390
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCATGCACATCTCGTATGCCGTCT5140.6019440215481907No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCATGCACATCTCGTATGC4780.5597845180934535No Hit
TCCGCCGGGGATATCTGTCTTTCCGTGTCTGGCTTAGTTCACTAAAAAAATG1550.1815200843190069No Hit
TTTAAAAAGAGATTAAACCGAAGGTGATTAAAAGACCTTGAAATCCATGACG1380.16161142990982552No Hit
GCTTATGGGACGCCCTTGTAATCCTAGCTACTTGGGAGTCTGAGGCGGGAGA1270.14872935940976695No Hit
ATATCCGGGAGGTAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGG1260.14755826209157982No Hit
GATGCTGGGGAATCAATTCAACAAGAAGAGCTAACTATCCTAAATATGTATG1250.14638716477339267No Hit
TTTATAGGGCAACACTCCACTTTCTGCATTGGGCAGATCTTGAAGACAGAAA1210.1417027755006441No Hit
AAGCGGGTAGAAGTCCTAGCAGAGCAAATAGACAAGAAAAAGAAATTAAAAA1180.13818948354608268No Hit
ATTTTAGGGCATAGGAGGAGGAAGAAAAGCTAAAAAACAAACAAACAAATAA1180.13818948354608268No Hit
TTAGTGGGGCCTAGGTTTCGAATTTCTGTCTTGTTACGATTTTTCTAACTTG1170.13701838622789553No Hit
GACTTTGGGGTCAGCTCACTGCAAGCTCTTCCTCCCGGGTTCACGCCATTCT1160.1358472889097084No Hit
GGTTCGGGGCAGAGCAGAAGCAGCTGCTGGAGGTGGAACTCTACGCAGACTA1130.13233399695514697No Hit
GATTTGGGGTGACGGAGGTTGAGATGAAGCTTCTTCATGGAGTAAAAAATGT1120.13116289963695985No Hit
CCAAAAGGGGAAGCCTGAAAAGGAGGAGCAACCTGTCCAGAATCCCCGCAGG1100.12882070500058557No Hit
CATTAAGGGAAAGAATAAGTATTGAAATGAAAGAATGACAGAAAGAATGAAT1090.1276496076823984No Hit
CTGTGTGGGGTGATTCAGTTCAGTAATGGTGATTCAGTTCAGTAACTGCCTA1050.12296521840964984No Hit
TGGAACGGGAACTCAACGACAATAATGACACAACCTATCAAACCTCTGGGAT1050.12296521840964984No Hit
ACTGGCGGGCAGACAGTGCTTGAGCTGCAGAAACTGAGACCAGACCTCTGGC1050.12296521840964984No Hit
AATTTAGGGACCTCACATTCTTCTACCGAAAAACAAAACAAACAAACAAAAA1030.12062302377327555No Hit
CAGAAAGGGGGGGAGAAACCAGAGCAGAAAAGCTGAAAATTCTAAAAACCAG1020.11945192645508843No Hit
CATAGCGGGTATATGTTTTGTCTCGCTATACTGCACTTACGCTATCCAGTTG1020.11945192645508843No Hit
GCAAAAGGGCACGCCACCCGCGTCATCGCCCTTATCAACGACCAGCGCCGGG1020.11945192645508843No Hit
GGGAAGGGGTGTCTGCAATGAGCTAGAGAGCTAGTTTTTTTTTAAATAAGGA1020.11945192645508843No Hit
TTTACTGGGGTTTGGAGACATCAGGGTGAACGAAAAGTCAGAGTACAGGATG1010.11828082913690127No Hit
AAGCAGGGGGGCAGTGCTAAGTAGATGACTGATGCTGAAATTTTACTAGATA1000.11710973181871415No Hit
ACGAAGGGAGACTGTGGGTGGATTTTCGAGCTGACGGTGGTCAATTCCTTTC990.115938634500527No Hit
CAAATAGGGGGAGCCACTGTGCACTTTGCCCAGCTGGCACGCTCACAGCTAC990.115938634500527No Hit
GCTTTGGGGCAACTTGGTCAATCTACATGGTGTGACAACAGATATAAGGAAA990.115938634500527No Hit
GTATTAGGGGCTGTCGTCCTGCAACCCCGAGACTCGCGACGAGACACAGCTC980.11476753718233985No Hit
GTCTAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA980.11476753718233985No Hit
TTATGGGGCATTATTAAAAAAAGAAACATCTCAGCCAACAAGGAATATAAGG970.1135964398641527No Hit
TCAAATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA970.1135964398641527No Hit
TCCTTGGGGGACGTACACCAATGCGGACAAGCTCCTAGAAGCAGCAGAGCAG960.11242534254596558No Hit
CGCCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA950.11125424522777842No Hit
GCAAATGGGACTTCACCAAACTATCTCTGCTAAACTCTGAGGATCTGGAGGT950.11125424522777842No Hit
GCAACTGGGATCCGACCTTAATCTGGGATAAAGGTTTTGGTATTTGCCCACT940.1100831479095913No Hit
TTAACTGGGATTTTGTAATTAACTGTCAAGCCTGTGGATGATTTTTTTGAAC940.1100831479095913No Hit
CTAGGTGGGATAATAGGCTTGAAAATTGATATCCTGTGGTGCTAAAGTACAG940.1100831479095913No Hit
TATAGAGGGCTCAGTGGGAAACTTTATTTGGTTGATTTGGCTGGGAGCGAAA930.10891205059140414No Hit
TTCCATAGGAAGAAATTATCGAATTAAAACTTAATGTATGTCAATTATTTTC930.10891205059140414No Hit
TGTAACGGGCCGATCTACAATGGGGTAGACCCCAGTGGTCACCTCAAGGACA920.107740953273217No Hit
ATAACTGGGAGCAGCCAAGATGTTCAGTAAAGCCTTTGACAGTGGCATTATC920.107740953273217No Hit
TTCGTAGGGGGTGAAAGAAATCCTGTCAAATTAGTTCCAGGAAGGGTTTGCT920.107740953273217No Hit
AATTACGGGACTTTGTAGAGATTGACTTCCTAAGCTACTTAAGACAACTTGC910.10656985595502985No Hit
TAGTATGGGATGGGAAAGTGGTGGTGTTTCAGACTCAGCCCATAGTGGAGAG910.10656985595502985No Hit
CAAAGCGGGGAGCACAGGGAACAGGCCGCCACAGAAAAGGTTGCCTTTTATG910.10656985595502985No Hit
GCATGCAGTTTGGAAATAAAATTTAAACTCATTTATGTGTAATAAATATGTA900.10539875863684273No Hit
TGCGTAGGGACCCTCCTTGCATAAAATATCACACTTGGGTAAGTGCAGTATG900.10539875863684273No Hit
TCGCTCGGGCTACTTCTAAAAATTATTTTGAAAACGAAAGGAATATGTTTGG900.10539875863684273No Hit
AGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG900.10539875863684273No Hit
TCCAAGGGGACTTCTTTGGGGATTTTGGTTTCATGTTTGGAGGAACCCCTCG900.10539875863684273No Hit
GATACTGGGGTTACCTTAGGGGTCCTACTTAATTTTACTTATGGAATACTAA900.10539875863684273No Hit
AAACCAGGGGTTTTGGGTACATTGCCTTAAATTGCATTTTTGGTATTTGGGT890.10422766131865557No Hit
ATCGTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC890.10422766131865557No Hit
CGTACAGGGCAACAACATCCAAGCTGAGAGCCAAATCAAGAATGCAATCCCA890.10422766131865557No Hit
GCTGCAGGGATGAATTCTTTAGTTCTGACATATTATTTTCTATTTTTAGCAT890.10422766131865557No Hit
GGTACCGGGACTGGGAGAGGTGGTGTAACTTAGTTCTTGATGATCCCACTTC890.10422766131865557No Hit
ATGCACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA880.10305656400046845No Hit
GAGGTGGGAATATTTGGTTTGGTAGTATAATTTAGTTTAGCCACAAAGGCCC880.10305656400046845No Hit
ATGAACGGGGCGAAGTAGCTGTAAAGAGAGGCAGCATAATCAAAGATCTTAT880.10305656400046845No Hit
ACCACTGGGATAAGAAATATATTTTACAACATGACCCAAATACACACATCAG870.1018854666822813No Hit
GTACAAGGGGGTCTCAAACTCCTGGGAGCTCAAGCGATTCTCCTGCCTTGGC870.1018854666822813No Hit
TAGTCTGGGCACGAACACGTTTTTATTTTTCTCTTTTTATGAAGGCTTGCTC860.10071436936409416No Hit
GCCTAGGGGACAATTGACTTCCCTGAATTTCTGACAATGATGGCAAGAAAAA860.10071436936409416No Hit
CTTCAAGGGGGCAGCAAATGTTGCCGGGACAAAGTTTGAAATTGTTCGTTTA860.10071436936409416No Hit
CGGGCGGGGGGTTTCCGCGAGATCTAGCTGATATGCCTTTTTGAGATGATAA860.10071436936409416No Hit
ATGCTAGGGATTGAAATGGCTCTTAAACATGTAAGATACTCAACCTCAATCA860.10071436936409416No Hit
ATTCTTGGGATGACACTGACACTGCTATTTTGAAACCTGGAGGATGGAAAGG860.10071436936409416No Hit
GTCATGGGGATAAAAGGAAACCTTTTGTTGCTCCCAAGAAGAAAAGAAAAAA860.10071436936409416No Hit
CCATGGAGGGAAGTTTGAAAACCGATGAACCTTATTCTGATTATGTAGATAA860.10071436936409416No Hit
CTAGCTGGGAGAAAAGAGATATGTTTTCTGGAGTCATAAAGGAATTCAATTC860.10071436936409416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)