FastQCFastQC Report
Sat 31 Dec 2022
SRR5845534.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845534.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences250002
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGC8350.33399732802137583No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGCCGTCT8100.32399740802073584No Hit
TGCACGAGCTATTGAATTTCTCAACTTTGGTCATTTCACTGCTTTTTAAATC3970.15879872961016311No Hit
TCTTGGGGCCCCGCGGGAGTCGCGGCGCTGCGGGTAGGACCCGGGTTGCGGG3890.15559875520995833No Hit
TGCCAGGGAAGAGCAACAAGAACCGCATGGACTTCATCCACATGGTGATCGG3800.15199878400972794No Hit
GCTTGCGGGGAGTTTGATCACACAATTCATAATGTTGCTGCTTTCCATTAAC3600.14399884800921592No Hit
ACTGCAAGGCTGCCTCTGGGTCCATTCTGTGGGCCACTCTCCCCAACGTTCT3470.13879888960888315No Hit
TAATTAGGGAAGGAGAGTCGTGAAGCAGTTGAGAAAGAATTTGAGCCTCTGC3380.13519891840865272No Hit
GCTCACGGGCTACCCTGGGAGCACTGTGGCAACTGGTGGAACACAGAACTCT3370.1347989216086271No Hit
GCTAAGGGGGCACTGTGGTTCGACCGGAAAAGAAGCGGGTGACCCACAATCC3340.13359893120855035No Hit
TGGCCCGGCCTATTTAAGGTTCTAAATTTGCCTTGATTTGGGCATTTTTGTT3320.13279893760849915No Hit
AAGCCTGGGAGTGTGGACTGGAGCCGAGATGGAGGCCTCATTTGTACCTCCT3320.13279893760849915No Hit
TTTCCCGGGGAGATACACAATTTTACATAATAAATTAGCTAATTTTGGTGGT3320.13279893760849915No Hit
CACCAAGGGCGATCTACTGCTGGGTAAGGAGAGTCGTGAAGCAGTTGAGAAA3310.13239894080847353No Hit
GCAGCCGGGGAAACAGGTTGGTCATTAGCTTCACAATTTTGCCTAGAAATGA3280.13119895040839671No Hit
AGTAATGGGCTTGTCCATGTGCTAAGATGAATAGAACACAAGGAAATAAAAA3270.13079895360837113No Hit
ATTAGCGGGTGGCCACGTGTTTCCAGTGGCTGGACCACGCCGACACCTGGGA3270.13079895360837113No Hit
AGCTTAGGGGGAATAGAGGACACGACCAAGATGGCGGCGGTGTCTGGCTTGG3250.12999896000831992No Hit
AGCACAGGGAACTCTGAGACTGAGAAACTACGAAGTGACTTAGAACGTCTTC3240.12959896320829434No Hit
CAACGGGATTGAATCAAAAAAACGAGTGAGAATACATCACTCCAAATTTTAA3240.12959896320829434No Hit
TGCTCTGGGTCCACAGCTTTGGTGGGGGAACTGGTTCTGGGTTCACCTCGCT3240.12959896320829434No Hit
ATCATGGGGGGAATCCACTGTAGGAAAAACTAAAAGTATATGCTTTCTCATT3190.12759897920816635No Hit
ATTGTTGGGAGAAATTTTGGATTTTTCATCACTTTCTCTTTAGAAAAAAAGT3170.12679898560811512No Hit
TATTCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA3160.12639898880808953No Hit
TTCCATGGGGATTGATGTGCACTGCATTAGAAATGAGGTAGCTGACACAGAA3150.12599899200806391No Hit
TTCTGGGGGGAAGAACGCAAAGCTGAGAACATGGACGTTAATATCGCCCCAC3090.12359901120791034No Hit
GATATAGGGTAACCATTTTAACTGTATTTTTTGCAGCCCGTACCTTCTTGGG3090.12359901120791034No Hit
CTTTCAGGGAGGAGGGATTTAAGAGGGCAACAAGTAAAAAAACAACCTTAAA3060.12239902080783352No Hit
TACTGGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC3050.12199902400780793No Hit
GCCAACGGGATAGTTTATGAAGCTTTGACAACAAATGTAAACAGACACGAAA3050.12199902400780793No Hit
TTGAAAGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA3040.12159902720778233No Hit
CGATAAGGGGAGGTTCCTCACTGATCAATACTCTTGCATTGTATTTTATTTC3040.12159902720778233No Hit
ATACTTGGGGCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTG3030.12119903040775674No Hit
CGTTGGGGGACTGAGGCCTGGATAAAGCATTCACACAAAGATAAATGGATTG3030.12119903040775674No Hit
TGCGCTGGGATGTTAACATGATTCATAATATCATAAACATTTGCAGATGAAT3020.12079903360773113No Hit
TTTCTCAGGGCATATTATATTTTGCATTTATGTATTATGCACTTTCCTGTAT3020.12079903360773113No Hit
CAAATTGGGATTTTAGACAAATAATTATCTGTGTACAGATATTTGATATACT3010.12039903680770554No Hit
ATGAAGGGGGTACGTATATTAATACATTTGGCTTTAATTTTTTTAACTTTAG3000.11999904000767993No Hit
CAACAAGGGGTATCCAGGAGAGGAACTGCATAACCAAAGGGGTGACCAGCCC3000.11999904000767993No Hit
CAATATGGGAAGCGGGGGCAGGGGAAAGACACCACCCTCGCCCCAAGACTGG2990.11959904320765434No Hit
CTTGAAGGGTACAAAATGAATATACATTGAATGTGAATATTTTATTGATCCA2990.11959904320765434No Hit
TGTGCCGGGGATCTGTAGTCGGGCAGCAATTGAGCTGAATAAAAGAATAGAA2970.11879904960760315No Hit
GTAAAGGGGATGGTGAAAATGGTACATTTTATGTTATGTGTATCTTATGAAA2970.11879904960760315No Hit
GGTATCGGGCTGTTGACATGGAATTTGCTAAGAATATGTATGAACTGCATAA2950.11799905600755195No Hit
TGTCTCGGGCTTAATAGTCGGTTTCATGGAGATGAAGGATGGGAGATTAAGA2950.11799905600755195No Hit
TGGCCTGGGTACTCTTCTTGTATGTGTTTCCATATATCTCTATGGATTACCC2950.11799905600755195No Hit
TATATGGGGAAGTCCAAGAGCAGCGAAAGTCTGTCTTTGACCGGCATGTTGT2950.11799905600755195No Hit
AATCGTGGGGAGACCATGGAAAAAGCTGTAGAAGAAAAGATTGAATGGCTGG2940.11759905920752634No Hit
CTTACTCGGGGTCCGATCTAAGCTTGGGGAGCCGAGCCGTCCGCGCCCGGCG2940.11759905920752634No Hit
TCTGCAGGGAGGGCTAACACTGGCAATATGGCAGCTTTAAATTCTGAGTTAC2930.11719906240750073No Hit
TCTATCGGGGGGGGATCCAAAAGGAGCTGCAATTTTAAAGTCTTCTGATGTC2920.11679906560747515No Hit
GTACCCGGGAGGAAGGTGGGACGTTGGAGGGGTGGCCGCATGGAGTGAGTAC2920.11679906560747515No Hit
GTATCAGGGCAAACAACCAATTCAAATGAATGATATCCAAATATCATATAGA2910.11639906880744953No Hit
GACTCAGGGAGCAACAGAGCACTAAGTTGAATATCTCCGATTCAGTAAAACA2910.11639906880744953No Hit
TCAAAGGGGCAAGCAAGAATGAAGTCTTTTGATGTTTATGTTCATTTTAAGA2910.11639906880744953No Hit
CCACACGGGATAGTGGGAAGGGGACGCGCGCCCTGCGGCCCGGCCTCTAGTC2900.11599907200742395No Hit
TGCGGTAGGGATCCCATACATTGGGACAGACCTAGTTCAATGAATCTGAGGA2890.11559907520739833No Hit
AGTGACGGGCAGCAAGGGCTACTGCCACACAGTAGCTGTTAGCCCACAGGCA2880.11519907840737274No Hit
CACACGGGGGCGCGGCGGCGACGGCACCAGCATGTGGGCGGACCTCTGCAAG2880.11519907840737274No Hit
GGCTCTGGGATAACAAGTGTGATTGTATTTCCATTAACCTGAAATTTACTGC2870.11479908160734714No Hit
TATGGAGGGATGTGTGGGGGATTTTCAATAAATTTTCTGCAAAGTCTTAAAC2870.11479908160734714No Hit
TTTTGTGGGAAGTGTGTGTATATCTGGAGCCCCACACATTGTAATAAACACA2860.11439908480732155No Hit
TTAGCTGGGATGGTAAGGCCCCTAAAATAACTGAATTAATTGTTTATTGTAA2860.11439908480732155No Hit
TTTCATGGGGCCGAGAAGATGGTTAGGGAGGCAGAGCAGCTGCGCTTCCTGT2860.11439908480732155No Hit
TTTCTTGGGCTCCCTGCGGTGATCACAAGGGTTAAGGGGAATGAAAGTAGCT2860.11439908480732155No Hit
CGGTAAGGGCCGCCTCGCACCCGGAACAACAAAGCAAGGAAGACGGAGTCCG2850.11399908800729594No Hit
CACACAATGTTCAAGTCTTTTTCTCTTTCTTATTGATCATCTCTTCCTGTTT2850.11399908800729594No Hit
TCTGCCGGGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTTC2850.11399908800729594No Hit
TTACCAGGGGTCACATACTTAGTAACTGCCACATAAATACTGTTTGCTTGTA2850.11399908800729594No Hit
GCCAACGGGACCATGCATTGGGACAGCATAATTCTAATATTACATTGAATAT2840.11359909120727035No Hit
TCTATAGGGATAATAACAGGTTGTGGCAAATATGATAGATTGGCTTAACATC2840.11359909120727035No Hit
ATCAGGGGGGCAGACGGCGGAGAGCAGAGAGGGAGCGCGCCTTGGCTCGCTG2840.11359909120727035No Hit
AACAAGGGGAGCCCCGGAATGTGCGGGCCGTCATGAAGCGGGTGGTGGAGGA2840.11359909120727035No Hit
CGAAAGGGGCAAAATAACGAGAGCAGCTTAATAGAGCAAGCTCCAATACCTC2830.11319909440724474No Hit
TGCGCTGGGATGTTCCCCAGATTTGAGATATACTAAAGAAAGAAATTCAAAA2830.11319909440724474No Hit
AGACATGGGCAACCCGGGATTTCTTAAAATGCTTCACTTCAGGTTCTTGGTC2820.11279909760721914No Hit
GCGTCAGGGGTGATATTCTGTTGCTTTACAGTTACAGTTGATGTTTGGGGAT2820.11279909760721914No Hit
ATTGAAGGGAGAGGTGATTTGTCTTGGATGTTCTATTTATTAGAACTGGATC2810.11239910080719355No Hit
TTTGTGGGGAGGGACTGGGCTGTCACTCGTTGTTTTGCAGAAGAAGCCTTTC2800.11199910400716794No Hit
GCTTATGGGGAATTTCTGGGGTAAAACATAAACCTAGCTGAGTTGCCTTTAT2790.11159910720714235No Hit
ATTCATGGGGACCTTACCTGTTGATATTAATTTATATGACTTGATTTCTTTC2790.11159910720714235No Hit
TGTTCTGGGGGGTGTCCAATTTAGATAATTCTTCACATAAAATTTTCTCATA2780.11119911040711673No Hit
CGCTCAGGGACTGGCCCCAGGCGGAGGGGCTTGGAGCAGAGACGGCAGCCCC2770.11079911360709115No Hit
GTGCTGGGTCCCATAGTTTCTGATTGAGTAAGTCTGAGGTTGGACTCAGAAA2770.11079911360709115No Hit
TCGTGTGGGTGAGAAGTCAAATCTGAATCGACAGTTTCTTTTCCGGCCCTGG2760.11039911680706554No Hit
GTAACGGGGCAAAACTAAGCAGGGAGAATAAAGATAATTGTATTTTATGTTT2760.11039911680706554No Hit
TGAATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2760.11039911680706554No Hit
CACCTAGGGATCTATTTCTGAGTTGAATACTCAGCTCCCACGAGAAAGAAAG2750.10999912000703996No Hit
TCGCATGGGATTAGATGTGTGCCAGAATATTGTAAATTTTAACATAACTTTT2750.10999912000703996No Hit
AGCATAGGGGCAAGGCGACGGCCTCGCCCTTGTGCTGATCCTGGCGTTCTGT2750.10999912000703996No Hit
TCTCTGGGGTGTATCCGCGGCCGTAGCAGCCGGGCTGGTCCTGCTGCGAGCC2750.10999912000703996No Hit
ATGTTGGGGGTCTTGAACTCCTGACCTCAGGTGATCTGCCTGCCTTGGCCTC2750.10999912000703996No Hit
AAAATAGGGTGCTGCCTTTATGGACATGGGTACCAATTGCTTTTGTAATATG2740.10959912320701434No Hit
TTTTTAGGGATTGAAATAGCAGGAGCATGTTGATTTCTTGGTTAGGTTGCAT2730.10919912640698876No Hit
GTCGAGGGGCCTGCCCTGGGAAGGGCAGTCTCTCCCCAGTCAGTGAGGTCCC2730.10919912640698876No Hit
CTTATGGGGAGGCAGAGGTTGCAGTGAGCCAAGATCATGCCACTGCACTCCA2720.10879912960696314No Hit
TACAAGGGACTTGATGTGAAGATGTCATGCTATCACAATATGCTAAGAGCTC2720.10879912960696314No Hit
CCTGCTGAGGACGAGGAAGAGGAGGAATCCTTCCCACAGCCAGTAGATGATT2710.10839913280693754No Hit
AAGCTAGGGGGGCAGAATTTGTTTTTAATTCTGTCAGGGCTCAAGGAATTAT2710.10839913280693754No Hit
GCACTGGGGAGTAGCCACCGGTAATAAGCCAAGGGCTAGGCTCTTGTTTGAG2710.10839913280693754No Hit
AACACAGGGCACCTGTAGTCCCAGCTACTCAGGAGGCTGAGGCAGGGGAATC2710.10839913280693754No Hit
GCTGAGGGGACCCTTGTATTGCATCAGCAGGTTGGAAACTCTCTGCTTGTAG2700.10799913600691195No Hit
CTAGCATGGGAGGGTCAAAGCAGCAGTAACTACATGGACTTTTAAGTGCGGT2700.10799913600691195No Hit
CAAACAGGGACACCATTTTGCAAAAATTAGCTCAAAATGGATCAATGACCTA2700.10799913600691195No Hit
CTGTAGGGCTGTCAGTGTGGACGCACTCACAATCCACAACCACCTGTGGCAG2690.10759913920688634No Hit
TCCTACTGGGAAGTGGCCGCACTCCAGACCTGAAGGCTCTACTCAACGTGGT2690.10759913920688634No Hit
TGCGTGGGATCTAAACAATCTATGAAAAATTTTTTAAATTTAACTTTTTTTG2670.10679914560683514No Hit
ATTATGGGAGACAAACAGATAAACAAACGGAATCTGGGAGAGGAAGCATTAA2660.10639914880680955No Hit
AAGCATGGGCAACAGATAATGTACTTAATGTGGAAACTTAAGAAAAATAATT2650.10599915200678395No Hit
AAAGAAGGGAGATCGTGTTTGTGGCTGTGTTCTTCAGGATATTTTCCCTCAC2650.10599915200678395No Hit
GCTATCCTTCCCATTCTAAGTGATAATTTTTATCATTTCTGGAGATTTTTCC2640.10559915520675836No Hit
GCAAAGGTGTCTGGGTACTGGTATTTTTCCCCCCAAGGAGGGTTGGAAGTCG2640.10559915520675836No Hit
ATAGTGAGGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAA2630.10519915840673275No Hit
CGTTGCGGGATTTGCCTAAATACTTTTTTCTATTTTGTTAGATTTTGCCTTT2630.10519915840673275No Hit
ACTTTAGGGATATGAATACAAATCGAAGGCCTCTACCTCCTGGAGTTATACA2610.10439916480668154No Hit
CTTCACGGGTATAACAACAAAGGAATTGGGAACTGTAATGAGATCTCTTGGG2600.10399916800665594No Hit
TATTCCGGGAGACTTGGGAAGACCCAGGGCACAATGATAAACATACTGGCTG2590.10359917120663034No Hit
GTAATGGGGTACCTGCCTCTGGAGGGTTACCTGTTCCCAGAGAGTTACCTGC2590.10359917120663034No Hit
GGTTTGGGAGACCGCGCTGGGTTGCCGCTGCCGCTGCCGCCATCGTGCCAGC2590.10359917120663034No Hit
CAGTCAGGGATACAGTATGACTGTAGGTCTTTCAATTTAGTTGTTGTACTAT2590.10359917120663034No Hit
GTATGTGGGGCTCTTCCGATCTTTGTTGGGACTCTGAGTTATTACTTACTGT2590.10359917120663034No Hit
AACGCTGGGGATCTATAGTCGGGAATGAACTAAATAAGGCACCAGGGACCAA2580.10319917440660474No Hit
ACGCCAGGTTATGACTTTGCATAAGAACTGTTCATCTTTGGATCTTGAGCAC2580.10319917440660474No Hit
TACTTGAGGGACAAAGCATACATTGAGCTGTAGTAAGGCTGAGGAAGAGTAC2580.10319917440660474No Hit
AGTGTCGGGGGACTAAAAATCCAAGCAGCAAAATTTAATATTGATGGGAATA2570.10279917760657915No Hit
TATATCGGGGAGTCATGAATTTATTTTTACATGCTTATGCATGCATCTTTAC2570.10279917760657915No Hit
AATGTCGGGCACCACTGAAGAGTTTCATATACACCAAATAATGTCTCATCTC2570.10279917760657915No Hit
ATTCCAGGGAGTACAGGGAATATAAAATTGGTTTCCTGTGGTCATGATCAAG2560.10239918080655354No Hit
TTTTGGGATGACCCACCAATCACATGCCTATCATATAGTAAAACCCAGCCCA2560.10239918080655354No Hit
TTTGTAGGGATTGAAATGTAATTTAGGATGATTTGATCTTCCATCTCAAGTT2560.10239918080655354No Hit
GATACCGGGGCGCGTGCTGTTGGGAGTTGCTTGGAGGTTGGCGGCGCGGGGC2550.10199918400652795No Hit
CCCCAAGGGCAGAAGATGGAGAGGATTGACCAGAGGAATAACTTAGCTTGCA2550.10199918400652795No Hit
CTGCGGTGGGATCGTCTATTTGTGTGTACTTTTTCTTTGTGCATAACTTTAT2550.10199918400652795No Hit
GCTAATGGGCAAGGCACTACCTTGGCCAAAGGTAATCACTCTCCTGACTTCT2540.10159918720650235No Hit
TAGCACGGGAAGAATACTGTCTTCTGCAACTTAGTGTTGCTATTCAGTGAAG2540.10159918720650235No Hit
TATCTGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAATG2540.10159918720650235No Hit
TTATGTGGGGAGATTGTTATCTATATCTAGTCTCCTTTCCATATTGAACTGC2540.10159918720650235No Hit
AGCACGGGTGAAGCCACTTCAGCAGCAGCAGCAAAAGGCGAACTCATCTCTA2520.10079919360645115No Hit
GACCGGCTGGATCAAGTTGGGAGGCTGAGCAAACTCATATTCCAGTTAGTTG2520.10079919360645115No Hit
GTATGCGGGAACTGAGGAATGTTTAATATGAAAACATACATCGGATATGTGA2520.10079919360645115No Hit
AATGGTGGGATATAAGGTAATGATGATCCATACACTTGCTCTTTTAGAGATG2520.10079919360645115No Hit
TTTCGGGGGATACTCTGAAGTGTCAGGCTTCCAGTATATATAGCAACGCTCT2520.10079919360645115No Hit
AGATGTGGGAGCCAAATTGGTAGAGGAAGGATGCAAAGTATAAATTTGTGTA2510.10039919680642556No Hit
GTGCTTGGGATCACTTGCAAAAATAGCACGTGCTCAATCCTAGATTAAAATA2510.10039919680642556No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)