FastQCFastQC Report
Sat 31 Dec 2022
SRR5845532.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845532.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243648
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGCCGTCT8580.3521473601260835No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGC7600.31192540057788287No Hit
TGGCCCGGCCTATTTAAGGTTCTAAATTTGCCTTGATTTGGGCATTTTTGTT3810.15637312844759654No Hit
TGCACGAGCTATTGAATTTCTCAACTTTGGTCATTTCACTGCTTTTTAAATC3540.1452915681639086No Hit
GCTAAGGGGGCACTGTGGTTCGACCGGAAAAGAAGCGGGTGACCCACAATCC3460.1420081428946677No Hit
TTTCCCGGGGAGATACACAATTTTACATAATAAATTAGCTAATTTTGGTGGT3380.13872471762542685No Hit
GTAAAGGGGATGGTGAAAATGGTACATTTTATGTTATGTGTATCTTATGAAA3230.1325682952456002No Hit
CAACGGGATTGAATCAAAAAAACGAGTGAGAATACATCACTCCAAATTTTAA3200.1313370107696349No Hit
AGTAATGGGCTTGTCCATGTGCTAAGATGAATAGAACACAAGGAAATAAAAA3190.13092658261097978No Hit
ATCATGGGGGGAATCCACTGTAGGAAAAACTAAAAGTATATGCTTTCTCATT3190.13092658261097978No Hit
TTTGTGGGGAGGGACTGGGCTGTCACTCGTTGTTTTGCAGAAGAAGCCTTTC3180.13051615445232465No Hit
TGGCCTGGGTACTCTTCTTGTATGTGTTTCCATATATCTCTATGGATTACCC3150.12928486997635932No Hit
GCTTATGGGGAATTTCTGGGGTAAAACATAAACCTAGCTGAGTTGCCTTTAT3130.1284640136590491No Hit
GCTTGCGGGGAGTTTGATCACACAATTCATAATGTTGCTGCTTTCCATTAAC3110.1276431573417389No Hit
CAATATGGGAAGCGGGGGCAGGGGAAAGACACCACCCTCGCCCCAAGACTGG3100.1272327291830838No Hit
CTTGAAGGGTACAAAATGAATATACATTGAATGTGAATATTTTATTGATCCA3100.1272327291830838No Hit
GTTCACGGGGGTTAATATCACTAAATCTCAATTTCTGTGCCTGCAAAATGGA3080.12641187286577357No Hit
CTTCACGGGTATAACAACAAAGGAATTGGGAACTGTAATGAGATCTCTTGGG3070.12600144470711847No Hit
TCTGCAGGGAGGGCTAACACTGGCAATATGGCAGCTTTAAATTCTGAGTTAC3070.12600144470711847No Hit
GATATAGGGTAACCATTTTAACTGTATTTTTTGCAGCCCGTACCTTCTTGGG3070.12600144470711847No Hit
CACCTAGGGATCTATTTCTGAGTTGAATACTCAGCTCCCACGAGAAAGAAAG3040.12477016023115313No Hit
CACCAAGGGCGATCTACTGCTGGGTAAGGAGAGTCGTGAAGCAGTTGAGAAA3040.12477016023115313No Hit
TTGAAAGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA3030.12435973207249804No Hit
TGCTCTGGGTCCACAGCTTTGGTGGGGGAACTGGTTCTGGGTTCACCTCGCT2990.12271801943787759No Hit
TCTATCGGGGGGGGATCCAAAAGGAGCTGCAATTTTAAAGTCTTCTGATGTC2980.1223075912792225No Hit
TTTTGTGGGAAGTGTGTGTATATCTGGAGCCCCACACATTGTAATAAACACA2970.12189716312056738No Hit
TTCCATGGGGATTGATGTGCACTGCATTAGAAATGAGGTAGCTGACACAGAA2970.12189716312056738No Hit
ACTGCAAGGCTGCCTCTGGGTCCATTCTGTGGGCCACTCTCCCCAACGTTCT2950.12107630680325716No Hit
CGTTGGGGGACTGAGGCCTGGATAAAGCATTCACACAAAGATAAATGGATTG2940.12066587864460204No Hit
GTGCTTGGGATCACTTGCAAAAATAGCACGTGCTCAATCCTAGATTAAAATA2940.12066587864460204No Hit
AGATGTGGGAGCCAAATTGGTAGAGGAAGGATGCAAAGTATAAATTTGTGTA2920.11984502232729184No Hit
CACACGGGGGCGCGGCGGCGACGGCACCAGCATGTGGGCGGACCTCTGCAAG2920.11984502232729184No Hit
TGCCAGGGAAGAGCAACAAGAACCGCATGGACTTCATCCACATGGTGATCGG2900.1190241660099816No Hit
TCGCATGGGATTAGATGTGTGCCAGAATATTGTAAATTTTAACATAACTTTT2900.1190241660099816No Hit
ATAGACGGGCATAGGAATGCAAATGAATTACTCCATATAAATACAATCGACT2890.1186137378513265No Hit
TTAGCTGGGATGGTAAGGCCCCTAAAATAACTGAATTAATTGTTTATTGTAA2890.1186137378513265No Hit
TGCGGTAGGGATCCCATACATTGGGACAGACCTAGTTCAATGAATCTGAGGA2890.1186137378513265No Hit
TAATTAGGGAAGGAGAGTCGTGAAGCAGTTGAGAAAGAATTTGAGCCTCTGC2880.1182033096926714No Hit
ATTCATGGGGACCTTACCTGTTGATATTAATTTATATGACTTGATTTCTTTC2880.1182033096926714No Hit
AAAATAGGGTGCTGCCTTTATGGACATGGGTACCAATTGCTTTTGTAATATG2880.1182033096926714No Hit
AAGCCTGGGAGTGTGGACTGGAGCCGAGATGGAGGCCTCATTTGTACCTCCT2870.11779288153401628No Hit
AAGCATGGGCAACAGATAATGTACTTAATGTGGAAACTTAAGAAAAATAATT2870.11779288153401628No Hit
ATTGTTGGGAGAAATTTTGGATTTTTCATCACTTTCTCTTTAGAAAAAAAGT2870.11779288153401628No Hit
ATACTTGGGGCTTTTCACAAGATGGCGCCGAAAGCGAAGAAGGAAGCTCCTG2870.11779288153401628No Hit
TCTTGGGGCCCCGCGGGAGTCGCGGCGCTGCGGGTAGGACCCGGGTTGCGGG2860.11738245337536118No Hit
TATATGGGGAAGTCCAAGAGCAGCGAAAGTCTGTCTTTGACCGGCATGTTGT2850.11697202521670606No Hit
GCAGCCGGGGAAACAGGTTGGTCATTAGCTTCACAATTTTGCCTAGAAATGA2850.11697202521670606No Hit
AGTGACGGGCAGCAAGGGCTACTGCCACACAGTAGCTGTTAGCCCACAGGCA2850.11697202521670606No Hit
TATCGAGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGAA2840.11656159705805097No Hit
GTACCCGGGAGGAAGGTGGGACGTTGGAGGGGTGGCCGCATGGAGTGAGTAC2840.11656159705805097No Hit
TGAATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2820.11574074074074073No Hit
CGTTGCGGGATTTGCCTAAATACTTTTTTCTATTTTGTTAGATTTTGCCTTT2820.11574074074074073No Hit
AAAGAAGGGAGATCGTGTTTGTGGCTGTGTTCTTCAGGATATTTTCCCTCAC2800.11491988442343053No Hit
CTACTTGGGGTTTTGTAGTTTAGCGACTTCCGTATACATAAAGGGACATATT2780.11409902810612031No Hit
CAACAAGGGGTATCCAGGAGAGGAACTGCATAACCAAAGGGGTGACCAGCCC2780.11409902810612031No Hit
TAGCACGGGAAGAATACTGTCTTCTGCAACTTAGTGTTGCTATTCAGTGAAG2770.11368859994746519No Hit
TCTGCCGGGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTTC2750.11286774363015498No Hit
GTAACGGGGCAAAACTAAGCAGGGAGAATAAAGATAATTGTATTTTATGTTT2750.11286774363015498No Hit
GTATCAGGGCAAACAACCAATTCAAATGAATGATATCCAAATATCATATAGA2740.11245731547149987No Hit
GCTCACGGGCTACCCTGGGAGCACTGTGGCAACTGGTGGAACACAGAACTCT2740.11245731547149987No Hit
TACTTGAGGGACAAAGCATACATTGAGCTGTAGTAAGGCTGAGGAAGAGTAC2740.11245731547149987No Hit
GGTATCGGGCTGTTGACATGGAATTTGCTAAGAATATGTATGAACTGCATAA2730.11204688731284478No Hit
TTAACCGGGGGAATGTTGGCTCTGAACAAACTTTTGAAAACTTGGTTGACAA2730.11204688731284478No Hit
CACACAATGTTCAAGTCTTTTTCTCTTTCTTATTGATCATCTCTTCCTGTTT2730.11204688731284478No Hit
CAGTGTGGGGCTACGCAGGGATGGGCAGTTGCACGAAGGCATGAATGTATTG2730.11204688731284478No Hit
CAAATTGGGATTTTAGACAAATAATTATCTGTGTACAGATATTTGATATACT2720.11163645915418965No Hit
CTTTCAGGGAGGAGGGATTTAAGAGGGCAACAAGTAAAAAAACAACCTTAAA2720.11163645915418965No Hit
ATGAAGGGGGTACGTATATTAATACATTTGGCTTTAATTTTTTTAACTTTAG2720.11163645915418965No Hit
GCCAACGGGACCATGCATTGGGACAGCATAATTCTAATATTACATTGAATAT2720.11163645915418965No Hit
AATGGTGGGATATAAGGTAATGATGATCCATACACTTGCTCTTTTAGAGATG2720.11163645915418965No Hit
ATCAGGGGGGCAGACGGCGGAGAGCAGAGAGGGAGCGCGCCTTGGCTCGCTG2710.11122603099553453No Hit
GTATGCGGGAACTGAGGAATGTTTAATATGAAAACATACATCGGATATGTGA2710.11122603099553453No Hit
GCTTCGGGGATCTTAATTCGGGGTATATAAAATCTGTCCTTTCCTACCTGGA2700.11081560283687944No Hit
ATTCCAGGGAGTACAGGGAATATAAAATTGGTTTCCTGTGGTCATGATCAAG2700.11081560283687944No Hit
ATCAGAGGGCATCGTATCACTGATCTACAATGTGCTCAAGACCTTCATGGAG2680.10999474651956921No Hit
TCAAAGGGGCAAGCAAGAATGAAGTCTTTTGATGTTTATGTTCATTTTAAGA2680.10999474651956921No Hit
TATGGAGGGATGTGTGGGGGATTTTCAATAAATTTTCTGCAAAGTCTTAAAC2680.10999474651956921No Hit
GTCGAGGGGCCTGCCCTGGGAAGGGCAGTCTCTCCCCAGTCAGTGAGGTCCC2680.10999474651956921No Hit
TCTCTGGGGTGTATCCGCGGCCGTAGCAGCCGGGCTGGTCCTGCTGCGAGCC2680.10999474651956921No Hit
TGTTCTGGGGGGTGTCCAATTTAGATAATTCTTCACATAAAATTTTCTCATA2680.10999474651956921No Hit
AGCACAGGGAACTCTGAGACTGAGAAACTACGAAGTGACTTAGAACGTCTTC2660.109173890202259No Hit
TGCCGCTTGGGTGGTGCTGAAAAAAAATGTATATTCTGTTGATTTGGGGTGG2660.109173890202259No Hit
ATATAAGGGGTAGTCTTTAAAGCAATGTAATACACTTGCTGGATTTTAAATT2660.109173890202259No Hit
TTTCTCAGGGCATATTATATTTTGCATTTATGTATTATGCACTTTCCTGTAT2650.10876346204360388No Hit
AAGCTAGGGGGGCAGAATTTGTTTTTAATTCTGTCAGGGCTCAAGGAATTAT2650.10876346204360388No Hit
GGCTCTGGGATAACAAGTGTGATTGTATTTCCATTAACCTGAAATTTACTGC2650.10876346204360388No Hit
TTCTGGGGGGAAGAACGCAAAGCTGAGAACATGGACGTTAATATCGCCCCAC2650.10876346204360388No Hit
TATTCAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTA2650.10876346204360388No Hit
GTGCCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2650.10876346204360388No Hit
AATCGTGGGGAGACCATGGAAAAAGCTGTAGAAGAAAAGATTGAATGGCTGG2640.10835303388494878No Hit
GATTCAGGGTAAAACATTTATTTCAGGTTTAGTTTGCCTTTATCATCATCAT2640.10835303388494878No Hit
GACCGGCTGGATCAAGTTGGGAGGCTGAGCAAACTCATATTCCAGTTAGTTG2640.10835303388494878No Hit
TGCGTGGGATCTAAACAATCTATGAAAAATTTTTTAAATTTAACTTTTTTTG2630.10794260572629366No Hit
AGCTTAGGGGGAATAGAGGACACGACCAAGATGGCGGCGGTGTCTGGCTTGG2630.10794260572629366No Hit
TCGTGTGGGTGAGAAGTCAAATCTGAATCGACAGTTTCTTTTCCGGCCCTGG2630.10794260572629366No Hit
ACTTTAGGGATATGAATACAAATCGAAGGCCTCTACCTCCTGGAGTTATACA2630.10794260572629366No Hit
TTTAGTGGGCCTATTTCTTTTTTCTATTAAATAATTTATGCAAAATGTTCTT2610.10712174940898346No Hit
TCTATAGGGATAATAACAGGTTGTGGCAAATATGATAGATTGGCTTAACATC2610.10712174940898346No Hit
GACTCAGGGAGCAACAGAGCACTAAGTTGAATATCTCCGATTCAGTAAAACA2600.10671132125032834No Hit
TCATACGGGAGCTGCCTCTGTTTTAAAGATTATCCCAATGTGGAAGATGCCC2600.10671132125032834No Hit
TTACCAGGGGTCACATACTTAGTAACTGCCACATAAATACTGTTTGCTTGTA2590.10630089309167325No Hit
TGTGCCGGGGATCTGTAGTCGGGCAGCAATTGAGCTGAATAAAAGAATAGAA2590.10630089309167325No Hit
AATGTCGGGCACCACTGAAGAGTTTCATATACACCAAATAATGTCTCATCTC2590.10630089309167325No Hit
ATTATGGGAGACAAACAGATAAACAAACGGAATCTGGGAGAGGAAGCATTAA2590.10630089309167325No Hit
AGAACTGGGGTTCTGTGTGATTCGTTTGTTGTTCTACTGGAATGCATTGTAT2580.10589046493301812No Hit
GCTGAGGGGACCCTTGTATTGCATCAGCAGGTTGGAAACTCTCTGCTTGTAG2580.10589046493301812No Hit
CGCTCAGGGACTGGCCCCAGGCGGAGGGGCTTGGAGCAGAGACGGCAGCCCC2580.10589046493301812No Hit
GCCAACGGGATAGTTTATGAAGCTTTGACAACAAATGTAAACAGACACGAAA2570.105480036774363No Hit
GTGCTGGGTCCCATAGTTTCTGATTGAGTAAGTCTGAGGTTGGACTCAGAAA2560.10506960861570791No Hit
GATTTGGGGGAGAAAAGAGATAGAAGTCGGTGGAGAAGTGGTAGTGATATAC2560.10506960861570791No Hit
GATTTAGGGAACATATATATGAGAAAATGCTCCACATCACCAATTATTAGGG2550.1046591804570528No Hit
GTTTTAGGGCTAATAGTATTAGGATGTAAGCAATATTAAGATGGTTTTTATT2550.1046591804570528No Hit
CAGTACGGGATTGCTCCAGGATTCTGGATGGGCTGCATTTACTGTGTGAAGG2550.1046591804570528No Hit
TATGATGGGAAATCTACTTTATAAAGGCACAAATATATTCACAGTAAAGGAA2550.1046591804570528No Hit
CACCAAGGGTCTGTGGGCCCTACCCTCCTGCTGAGCTGCCCCTGCTGCTTAA2550.1046591804570528No Hit
GCAAATGGGGAGTTTATTGTTTTAGGCTTTCTTGGACAAGGTTTTTACTTAG2540.10424875229839768No Hit
GCTATCCTTCCCATTCTAAGTGATAATTTTTATCATTTCTGGAGATTTTTCC2540.10424875229839768No Hit
TTGAAAGGGCAAACAGCACCATGAAGAGCATCATTAAAACTAAGAGAAAGTG2540.10424875229839768No Hit
CCAGGTGGGGGGGGCCTGAATATTCCAGAGCTGATGTGATGGGCTGTGCAGA2540.10424875229839768No Hit
ATTATTGGGGACAGAGTCTTGTTCTGTTGCCCAGGCTGCAGTGCAGTGGCAT2530.10383832413974259No Hit
AGCCATGGGACATGGAGACAACAGTAAACAGAGTAGGAACTAACCGTACTGA2530.10383832413974259No Hit
TGCGCTGGGATGTTAACATGATTCATAATATCATAAACATTTGCAGATGAAT2520.10342789598108747No Hit
GGCGCTGGGATCTATAATTTACCTAAGATTACAAATCAGAAGTCATCTTGCT2520.10342789598108747No Hit
GCGTCAGGGGTGATATTCTGTTGCTTTACAGTTACAGTTGATGTTTGGGGAT2520.10342789598108747No Hit
AGCACGGGTGAAGCCACTTCAGCAGCAGCAGCAAAAGGCGAACTCATCTCTA2520.10342789598108747No Hit
CGAAAGGGGCAAAATAACGAGAGCAGCTTAATAGAGCAAGCTCCAATACCTC2520.10342789598108747No Hit
AGGTTGGGGCGAGCGACCAGCAGGATGAAGAAGCAGGAGAAGGCAACTGAAG2520.10342789598108747No Hit
TTTCGGGGGATACTCTGAAGTGTCAGGCTTCCAGTATATATAGCAACGCTCT2520.10342789598108747No Hit
CTAAAGGGGACCCTAACCGTGCAAAGGTAGCATAATCACTTGTTCCTTAAGT2510.10301746782243235No Hit
GCATATGGGAGAACCGGTGGAGCAGCGACCCCTGAGCAGTGTTCTCTGTGCT2510.10301746782243235No Hit
ATGCCTGGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG2500.10260703966377725No Hit
CGGGTAGGGGCAATATTACATTGCCCTACAGAATTAGCACAACTTTCTTTAT2500.10260703966377725No Hit
GCACTGGGGAGTAGCCACCGGTAATAAGCCAAGGGCTAGGCTCTTGTTTGAG2500.10260703966377725No Hit
TATACAGGGCAAGCTGGGAAAGCTTATTTTTAACATAATTTTTTAGTGTGGA2490.10219661150512215No Hit
GACAGTGGGAGCTATTTATCACTTAACAGACTAGATTGATTCTCTGACACGT2490.10219661150512215No Hit
GATACCGGGGCGCGTGCTGTTGGGAGTTGCTTGGAGGTTGGCGGCGCGGGGC2490.10219661150512215No Hit
TCCTACTGGGAAGTGGCCGCACTCCAGACCTGAAGGCTCTACTCAACGTGGT2480.10178618334646704No Hit
TTTGTAGGGATTGAAATGTAATTTAGGATGATTTGATCTTCCATCTCAAGTT2480.10178618334646704No Hit
TAATAAGGGTACTTTATTAAATAAAGGGGGACAGGATGACAGTGCAGATATC2470.10137575518781193No Hit
GTGCAGGGGCATCTCATACCAGTCAGAATAGCTATTATGAAAAAGTCAAAAA2470.10137575518781193No Hit
CAAACAGGGACACCATTTTGCAAAAATTAGCTCAAAATGGATCAATGACCTA2470.10137575518781193No Hit
TTTCATGGGGCCGAGAAGATGGTTAGGGAGGCAGAGCAGCTGCGCTTCCTGT2460.10096532702915681No Hit
ATGTCTGGGGCAAAGAGTTTACCTTTAAGAGCAAGATCTGAACTTACTTTGC2460.10096532702915681No Hit
ACTGTGGGGACAGAATAACGATTGTGACCATTATGAATGTAACTAATTTCAG2460.10096532702915681No Hit
AAACCGGGCTCCCTTCCCTCATTGTCTAATCTACTTTCTGTCTCTGGATTTG2460.10096532702915681No Hit
GGGCCAGGGAAGCACAATCTGCAATAGTTTATGTATGACAGAGATAATTCAA2450.10055489887050172No Hit
AGCGCTGGGACATGTATGTTTATTGCGGCACTATTCACAATAGCAAAGACTT2450.10055489887050172No Hit
TTTCTTGGGCTCCCTGCGGTGATCACAAGGGTTAAGGGGAATGAAAGTAGCT2440.1001444707118466No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)