FastQCFastQC Report
Sat 31 Dec 2022
SRR5845528.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845528.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences243657
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGC10060.41287547659209467No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGCCGTCT9540.3915340006648691No Hit
TAACATGGGTGTATGACATCATACGAGACAATAAACAACATGAAAAGAATGT3320.13625711553536324No Hit
ACATAGGGACTAATGGAGTAGATATGTGGCATTCAACAAAGAGAGGTTCCAG3280.13461546354096127No Hit
AGCAATGGGAAATGAAATTAGAACTTCTGAAATTTGGGCTTGCTATGTAAAT3240.1329738115465593No Hit
TTATTGGGGCCTGGAAGAACTCAGAGGGGATGAAGGCACGAGGAGTCAGAGG3200.13133215955215732No Hit
CGTCAAGGGGATGTAATGAAATTTCTCTGTAATTCTAAACAACAAACATCAA3200.13133215955215732No Hit
GACCTCGGGGTCTGAAATGACAAGGGCAAGAGTGAGATAGGAAACTGTGTGT3140.1288696815605544No Hit
GATAATGGGTTTCTTCTTGCATTTCAAGATTGCTTTTGTTTTGGGGGGACAC3110.1276384425647529No Hit
CAGCGCGGGCTTATGCAGGGGGAATTTAAGAATAGGATTCATGGATTTCAAC3080.12640720356895144No Hit
TGTACCGGGATCGGGGGATCTGGTTGAATCTGAGATTTTGACTGGCTGGTTT3070.12599679057035096No Hit
GTTTGCGGGGAAGAGACCAATTAAGATTCATTCCTCATTCTGTTTCTTTCCA3050.12517596457314997No Hit
AAAGCGGGGCGATCTGTTGACGGGCAAGGACCAAAATGTCAAATTTAGGAAA3050.12517596457314997No Hit
TGACTGGGGGAGGAAGGGAGCTGAGGAAAGGAAGCAGGAGAAATGATTCCAC3000.12312389958014751No Hit
AAATACGGGGGTCCCTGGGAAAAAGATGAGGGCTGCTTCAGGCTGGTCTCTC2980.12230307358294652No Hit
CTCAGTGGGGTGTCCAGGATTTTTAGCTGTATTCAGTGGGAGAATCAGAGTG2970.12189266058434602No Hit
TTCTCAGGGCATTTGCCCACTTCTATGAATTTTATGTATAAAATTTTTTAAG2930.12025100858994404No Hit
TTACCTGGGGCAGAATTTAGTGTTATCAGTGATCTGGATTTTGGTATATAGT2930.12025100858994404No Hit
AAACCTGGGCTTTTGAGTGCCAAAAAGAGATAGATGGGTATCAGATTATAAG2910.11943018259274309No Hit
AATTGTGGGCATGATTAGGTGACTTATCCTGCATGTTAATAATTTGATTTTT2890.1186093565955421No Hit
TTTGGGGGCTCTTCCTCGCGGAAGTGGGGAGGAGGCGGTTGCGGTTAGTGGA2890.1186093565955421No Hit
GTTGTTGGGGTAGGAAGGCTGAAAAATGACGCCATGACAAATGAAATTGTCA2890.1186093565955421No Hit
AGCTTAGGGCAGAACCACACCCCTCGTACCAATGCTGCCATCTCGTTCAGGC2870.11778853059834112No Hit
TTCTATGGGATTTCTGGCTAGTGTCTGTTAATAAAACACACATACTTAACTT2870.11778853059834112No Hit
AAATCTGGGGTGAGCGGGGGCCACAGCCCGGGCTGGGGGGAGCCGGGGTGCA2860.11737811759974061No Hit
AATCACGGGTCTTTCGGCCGCGCTGGTGAACAGGACCCGTCGCCATGGGCCG2840.11655729160253964No Hit
CGAACTGGGGGAGGAAATAATTTCAGATAAGGTGGAAATAGCAGGAGAACTA2840.11655729160253964No Hit
GACAAGGGGAGGACCGAGTGGCCCCGCCAGGATTAACACAGATTCCTCAGAT2830.11614687860393914No Hit
AGTTACGGGAAGCTGCTCATCATGAACTGGCTGTTGTATGATCACCAAGCCA2820.11573646560533865No Hit
CAGACAGGGAGTGATCCTCCCACTTCAGCCTCGGAAAGTGCTGGGATTACAG2790.11450522660953717No Hit
TAACCAGGGGTACTGTGGATGAGTATCTGCAAGACCAGGTAATGACACATTT2780.11409481361093668No Hit
ACTCGCGGGCAGACTCTAAACACACAAGTAGATTGATCTAAGGCATGCTCCC2770.1136844006123362No Hit
TGTAAAGGGATTTTTTGAGGGGGAGTGAGTTTTAGGAAAGGGGAATTAAGAT2770.1136844006123362No Hit
CAATCGGGGAGGGTTCCCATTTGCACTTCATCTTTCAGTAAAGTCTTGTCAG2750.11286357461513521No Hit
AAGCCTGGACTGCATAGCAAGATCTTATTTGTACAAAAAAATGTAAAACTTA2740.11245316161653472No Hit
TATACTAGGGAATTTATCAGTGTTTTCCTTTTATAATTTGTACTTTGTGTCT2740.11245316161653472No Hit
ATGACAGGCACCTCACAGTGTCCATACAAATTAGTTCCAGAGGGAAGATATA2740.11245316161653472No Hit
ATCACTGGGATTGTGATTTTGATTTGCATTTCCCTGATGTCCAATAATGTTG2720.11163233561933374No Hit
GCAATTGGGGAGACCGAGCTGCAGAAAGACGGGAGAAGTACGGCATTCCAGA2720.11163233561933374No Hit
TGCAACCAGCTACTCAAAAGCTTCGACTTCCACTTTGGCTTCTGCATCCCCA2720.11163233561933374No Hit
ACCGGCGGGTACTTTTATCTGAGAATTCCTTTTGATATCTGACACCCATTTT2710.11122192262073324No Hit
GCACTGCGGGGGGGGCATATTGTAGTCCTGTCATTTAAGTTATGTAAAAAAT2710.11122192262073324No Hit
TGATTTGGGTCTATAGCTGGGATTAAAATGCCACTTGGGTGGAGTGGATAGT2700.11081150962213276No Hit
TTACGTGGGATCCCTGCCTCTACTGGCGCTGCCAAGGCTGTGGGCAAGGTCA2700.11081150962213276No Hit
GGTCCAGGGCGGTGACTACTACCTCCTCTAACCCTTTGGGTGTGTTTGCAGC2690.11040109662353226No Hit
ATTCTGGGGATGTGAATCCAGAGATAAGTTTATACGGGAATTATTTGAATCA2690.11040109662353226No Hit
CCAACAGGGATAAAGACAATTATGAACTGGATGAAGACACAGACTAAAAAGG2680.10999068362493178No Hit
TGGTGAGGGGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTAT2670.10958027062633127No Hit
TGATGTGGGGTACAATTAATTGTGTTCTTGTGACCTGATGATTTTTTGAAAA2660.10916985762773079No Hit
TCACCAGGGAAGACTGCAGAATTTGTCCAACTTGACATTCAGATTCTGCTTC2660.10916985762773079No Hit
ATTTACGGGATCTCACGAGCAGAGTCTCAGAGCCGCCAGAGGCCACCCCTGG2660.10916985762773079No Hit
CGTTTGGGGAGTTCCAAAGAGTAAACTCACAGAAGTCTTGAGGTTTCAAATA2650.10875944462913029No Hit
GATAGTGGGATCACTGGCTAATGAGAAAAGGAGACAGCTAACTCTAGGATGA2650.10875944462913029No Hit
AATTGAGGGACAGGCTGGTGATGTGTCTGCTTACATTCCAACAAATGTCATT2640.10834903163052981No Hit
GTTATAGGGCGATCTTATGTGGGGTAGTTGGAGAGGCATGGTTCTGTTTGTT2640.10834903163052981No Hit
ATTCTGGGGGGAAAACTTCAGACTTTGGCTTCATTTTTAATCTTTTTGGAGA2640.10834903163052981No Hit
TGCTGCCGGGATGTATAGATTTTCTTTTAATTATAAACAGCCAGATAGCCAG2630.1079386186319293No Hit
CAAAGTGGGCGTCACTTTTATGGCGTTAATTTCCTGCCACTACAGATCTTTT2620.10752820563332882No Hit
ATCAAAGGGAACTGCTATGACGGTTAAGAATGTCAGTATACAAGAAGGAATA2620.10752820563332882No Hit
GTGTAAGGGATAAAGCTAAAACTCACCTGAGTTGTAAAAAACTCCAGTTGAC2610.10711779263472834No Hit
CAAACCGGGTCTGTGTCAGGGAGTGGGCCCAGGCTGGGATCAGCAGGGAACT2600.10670737963612784No Hit
CAGGTTGGGATGAGTGTGGGCTATGGAAAGACAGCTTTTCCTACACTGATAA2600.10670737963612784No Hit
TTTAGGGGGAACTACTCCCTAGGCAGACAGACTAAAGCTTAGGCCAGCCATG2590.10629696663752736No Hit
TTATGGGGGCAAAATTCCAATGGCATTTCCTAAGGAAATAGAAAAACTAATC2580.10588655363892685No Hit
TGGGGTGGGAACACCCATTTGGGGTTACCATGTCCATCCCCCAAGGGGGGCC2580.10588655363892685No Hit
TTTGTGGGGAGTGCGAGATCCGCTGCTGCTGAGGAGAGGAGCGTCAACAGCA2580.10588655363892685No Hit
TTTATGGGATGTGTATGTGTGGAGTATGTGTGTTTGCTTCTCCGTGGATGAA2580.10588655363892685No Hit
GCATTGGGGAGCAGAGAGGTTGAAAGCACTTAACTCTTGTTTAGTATCCTCA2580.10588655363892685No Hit
TAATGCGGGGACTGTGGGAAAGGCCTCAAGACAAACTCAGACTGTTTTTTTT2580.10588655363892685No Hit
TAAAAAGGGACAATAAATGAGTTTCAATAACATTAGGAGGCAATTGAAGACA2570.10547614064032637No Hit
AGTTTGGGGCAGATATTTAAACATCTTGTTTTATTGAGTATAAAAACACATC2570.10547614064032637No Hit
TCTTTAGGGGATTTTTCCATGTGTTTCTGCATATTTCCACATTCTTCCTTCT2560.10506572764172586No Hit
GGCTCTGGGTTGCTGCTTTCCCAGTGCCAGGAGAACCCCCGCTCCGAGTCAG2560.10506572764172586No Hit
GATAGCGGGGTCAAGATTGGAAGTGGAACCTAAGTGTCAAGAGTTGACAGTC2560.10506572764172586No Hit
TCAAATGGGAAGTAGAAATTATGTTTTCGACATGGGATATTTTCACCTTACC2560.10506572764172586No Hit
GCTTTAGGGATCATCCACTTTTAATGTTTTCCATCCACATAGCTGCATTGCC2560.10506572764172586No Hit
TCGTGTGGGCAAAACAAAAAATTGTGGGTACATAGTAGGTGTATATATTTAT2560.10506572764172586No Hit
ATGTGCGGGTCAGTCGGTCGGCGGCGGCAGCGGAGGAGGAGGAGGAGGAGGA2540.10424490164452488No Hit
TGTGGGGGGGTCACTAGGTCACGGGGGCCTCACTTCATTCTCTTCCACGTCA2530.1038344886459244No Hit
GCACCTGGGGAGTTGAATCAAATTTTACTCACTATGAAAAGTACAACTGTCA2530.1038344886459244No Hit
TTCAAGGGAATGAACTTAAGTCTGTGAATCAGCCTTTGTGTTAACGACTGGT2530.1038344886459244No Hit
AGCAATGGGGAATGAATGAGTGAAAAAGGTTCCAAGTTATTAAAATGTTCTG2530.1038344886459244No Hit
GGTCTCAAACTCCTGACCTCAGGCGATCCACCGGCCTTGGCCTCCCAAGGTG2530.1038344886459244No Hit
GAATTAGGGGGGCAGCGTCTATATCACCTTGAAGAAGTATGACGGTCGAACC2520.1034240756473239No Hit
TTAGCAAGGAGTGACAGAAGTAGAGAAGAGAATGGAAAGCAGCACACTCCGT2520.1034240756473239No Hit
TATGCTGGGCGTTTTGAATTTGCATGACCAGAACTCCACTATCCATGCAAAC2500.10260324965012291No Hit
AACGTTGGGCAGATGTCTTATGTCTCCAGAAAATTCAGATCCACAATTCCAC2500.10260324965012291No Hit
GCTTATGGGTACATATAAAATGCACATTGAATTTCAAAGACTAGTAAGAAAA2500.10260324965012291No Hit
ACCAGTGGGCATCTTTGTTTGAAACATCGCAGGATAATGATACTTATTGAAA2500.10260324965012291No Hit
CTTGTCGGGGATCAAACAAACCTGTGAAAAATTTATGGTGAAAATATTACAT2500.10260324965012291No Hit
CACACACGGGAGCTGGGGGGAGAAGAACTTATACTAAACTACCAAGCTTATT2500.10260324965012291No Hit
CACAGAAGGGCGCGTTCGGTGATGCTGCATTACCGCTCAGACCTGCTCCAGA2500.10260324965012291No Hit
AACATCGGGCAGAAAATAAATGAAATTCATTAAGCCTTCCAATACAATGGCA2490.10219283665152243No Hit
CAGGAAGGGCAGTTACACAATGGGCTTTGAAAAAATCCAGGTTTTCCAACAG2490.10219283665152243No Hit
GCACAGGGGAGACTCACAGACTTAAAGTAGAAGCAAAACAAACATGATGGCA2480.10178242365292195No Hit
TAACTGGGATTGCTCTTAAACTTGGTGTGACTGCTAATGATGTAAAGAATGT2480.10178242365292195No Hit
GACTAAGGGGTTCTTTTTGCAAATAAAGTGCTCATTTTGGCTGTTTTCCAGA2480.10178242365292195No Hit
GGCTCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2470.10137201065432144No Hit
TTACATGGGGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT2470.10137201065432144No Hit
TCTCGAGGGAGTCTACCACCAGTGGCTAATGCTAGCTATGAGCCAGGCACTA2470.10137201065432144No Hit
CCGTCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2470.10137201065432144No Hit
GCAAGAGGGTCTGACACGGGGCTGAGGACTGGGGAGAAGCTGAGCCCTGCCC2470.10137201065432144No Hit
ATCAAAGGGCAAGCAAGAAATCAAGGTAATGTGGAGTTGGATATTAGCAGTG2470.10137201065432144No Hit
AAGTGCGGGAGGAAAAAACCTTGTAGAGAGAGTAAAAAATTTAACACCCATA2470.10137201065432144No Hit
AAAGAGGGGGATTCCAAGAATGCCATCTGATAAAAAAGAATAGAAATGGAAA2460.10096159765572096No Hit
GTAAATGGGTAGGAAGTACATTGAACTTCAGAATTTAACTGAGGTGAAGTGT2460.10096159765572096No Hit
CCATCCGGGAGGTTCTCGCGATTCAGGACGTCCTTCCATATCCCTGCCCAGC2460.10096159765572096No Hit
CATCTAGGGGGGAATTATGTGCTGTAGTATGTATATAGTATTTTTAGTGAAA2460.10096159765572096No Hit
TTGCCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2460.10096159765572096No Hit
GCACCCGAATCACTCGAAGCCAGCCCAACCACACACCTGCAGGCCCGCCTGG2460.10096159765572096No Hit
CATAGCGGGCTTCTTGGCTAATCTCTGTACTTCTGCCTTTTAATTACTGGGC2450.10055118465712046No Hit
GTCACCGGGAGAAGTCTATCATGTAGAGAAACATTTCTTTTCCCGTTCATTT2450.10055118465712046No Hit
ATGGCTGTTCAGCAGCAGTATGGTGTAGGAGATTTGTTCCATAGGTCTCTGG2440.10014077165851998No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)