FastQCFastQC Report
Sat 31 Dec 2022
SRR5845527.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845527.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences244167
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGC10040.4111939778921803No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGCCGTCT9070.37146706966952947No Hit
TAACATGGGTGTATGACATCATACGAGACAATAAACAACATGAAAAGAATGT3680.15071651779314976No Hit
TTATTGGGGCCTGGAAGAACTCAGAGGGGATGAAGGCACGAGGAGTCAGAGG3470.14211584694082327No Hit
AGCAATGGGAAATGAAATTAGAACTTCTGAAATTTGGGCTTGCTATGTAAAT3450.1412967354310779No Hit
TGTACCGGGATCGGGGGATCTGGTTGAATCTGAGATTTTGACTGGCTGGTTT3410.13965851241158717No Hit
GTTTGCGGGGAAGAGACCAATTAAGATTCATTCCTCATTCTGTTTCTTTCCA3380.13842984514696907No Hit
TGACTGGGGGAGGAAGGGAGCTGAGGAAAGGAAGCAGGAGAAATGATTCCAC3280.13433428759824217No Hit
CAGCGCGGGCTTATGCAGGGGGAATTTAAGAATAGGATTCATGGATTTCAAC3230.13228650882387874No Hit
CGTCAAGGGGATGTAATGAAATTTCTCTGTAATTCTAAACAACAAACATCAA3220.13187695306900604No Hit
AAATACGGGGGTCCCTGGGAAAAAGATGAGGGCTGCTTCAGGCTGGTCTCTC3220.13187695306900604No Hit
TGGCTGGGGCGACCTCACTGTCAGCGTTTCTCCACCTGAAGTGATGATGGAT3130.12819095127515184No Hit
TTTGGGGGCTCTTCCTCGCGGAAGTGGGGAGGAGGCGGTTGCGGTTAGTGGA3110.12737183976540647No Hit
AAACCTGGGCTTTTGAGTGCCAAAAAGAGATAGATGGGTATCAGATTATAAG3080.12614317250078838No Hit
GAATTAGGGGGGCAGCGTCTATATCACCTTGAAGAAGTATGACGGTCGAACC3020.12368583797155226No Hit
GACAAGGGGAGGACCGAGTGGCCCCGCCAGGATTAACACAGATTCCTCAGAT3010.12327628221667956No Hit
TATACTAGGGAATTTATCAGTGTTTTCCTTTTATAATTTGTACTTTGTGTCT3000.12286672646180687No Hit
ATTCTGGGGATGTGAATCCAGAGATAAGTTTATACGGGAATTATTTGAATCA3000.12286672646180687No Hit
AAGCCTGGACTGCATAGCAAGATCTTATTTGTACAAAAAAATGTAAAACTTA2960.12122850344231612No Hit
CGTTTGGGGAGTTCCAAAGAGTAAACTCACAGAAGTCTTGAGGTTTCAAATA2950.12081894768744345No Hit
CAGACAGGGAGTGATCCTCCCACTTCAGCCTCGGAAAGTGCTGGGATTACAG2950.12081894768744345No Hit
TTTAGGGGGAACTACTCCCTAGGCAGACAGACTAAAGCTTAGGCCAGCCATG2950.12081894768744345No Hit
CTCAGTGGGGTGTCCAGGATTTTTAGCTGTATTCAGTGGGAGAATCAGAGTG2950.12081894768744345No Hit
GACCTCGGGGTCTGAAATGACAAGGGCAAGAGTGAGATAGGAAACTGTGTGT2930.11999983617769804No Hit
TGTAAAGGGATTTTTTGAGGGGGAGTGAGTTTTAGGAAAGGGGAATTAAGAT2910.11918072466795267No Hit
TTTATGGGATGTGTATGTGTGGAGTATGTGTGTTTGCTTCTCCGTGGATGAA2900.11877116891307998No Hit
TGGTGAGGGGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTAT2890.11836161315820729No Hit
AGCTTAGGGCAGAACCACACCCCTCGTACCAATGCTGCCATCTCGTTCAGGC2880.11795205740333461No Hit
AATCACGGGTCTTTCGGCCGCGCTGGTGAACAGGACCCGTCGCCATGGGCCG2870.11754250164846192No Hit
GCACCGGGATACCATCAGAGACATCATTCACAAGTAACTGATGTATTGGCAT2870.11754250164846192No Hit
GTTGTTGGGGTAGGAAGGCTGAAAAATGACGCCATGACAAATGAAATTGTCA2870.11754250164846192No Hit
TGGGGTGGGAACACCCATTTGGGGTTACCATGTCCATCCCCCAAGGGGGGCC2860.11713294589358922No Hit
CGTTAGGGGTTATATCTACGTATAATTTTTGAAATACCCATAACACTTTGTT2860.11713294589358922No Hit
GGTCCAGGGCGGTGACTACTACCTCCTCTAACCCTTTGGGTGTGTTTGCAGC2850.11672339013871653No Hit
TTCTCAGGGCATTTGCCCACTTCTATGAATTTTATGTATAAAATTTTTTAAG2850.11672339013871653No Hit
AGAACTGGGGTTAGCGTAACTCTAGAGAAGGAAGGATCAAAGAAGCTTAAAC2830.11590427862897117No Hit
CTCTGGGGGGAGAGCCGCAGCGGCAGCGGCGCGTCCTGCCTGCAGAGAGCCA2820.11549472287409847No Hit
AACGTTGGGCAGATGTCTTATGTCTCCAGAAAATTCAGATCCACAATTCCAC2820.11549472287409847No Hit
AATTGTGGGCATGATTAGGTGACTTATCCTGCATGTTAATAATTTGATTTTT2800.11467561136435309No Hit
TACATTGGGAACTTAAATTGATACAATAAAAAAGAGAAACATCATTAGAACA2800.11467561136435309No Hit
CCAACAGGGATAAAGACAATTATGAACTGGATGAAGACACAGACTAAAAAGG2790.11426605560948039No Hit
CGAACTGGGGGAGGAAATAATTTCAGATAAGGTGGAAATAGCAGGAGAACTA2780.1138564998546077No Hit
GCACAGGGGAGACTCACAGACTTAAAGTAGAAGCAAAACAAACATGATGGCA2770.113446944099735No Hit
GTCACAGGGGTCTGGCTTGCACTGAATGCCCAGAAATGGAAGCGCAAGGAAA2770.113446944099735No Hit
TTACCTGGGGCAGAATTTAGTGTTATCAGTGATCTGGATTTTGGTATATAGT2770.113446944099735No Hit
CATAGGTGGGGCCCCCTCTGCTGATGCCCCCATGTTCGTCATGGGTGTGAAC2760.11303738834486234No Hit
TTAGTAGGGCACCTTTGTTCCTCAGCTAAAAGTAATTTTGTTATAAACACAA2760.11303738834486234No Hit
ATGGCTGTTCAGCAGCAGTATGGTGTAGGAGATTTGTTCCATAGGTCTCTGG2750.11262783258998964No Hit
ACATAGGGACTAATGGAGTAGATATGTGGCATTCAACAAAGAGAGGTTCCAG2740.11221827683511695No Hit
CCTGCAGGGAAGCTGCAGTGTATCAAAAGATGTGCCCAATGTTCCCATGAGC2740.11221827683511695No Hit
AGTTTAGGGGGGCTGAGTGCACAGGTGGTACTTAATAATGTAGACTAGATAG2730.11180872108024426No Hit
TTATGGGGGCAAAATTCCAATGGCATTTCCTAAGGAAATAGAAAAACTAATC2720.11139916532537156No Hit
ATGTGCGGGTCAGTCGGTCGGCGGCGGCAGCGGAGGAGGAGGAGGAGGAGGA2710.11098960957049889No Hit
CACACACGGGAGCTGGGGGGAGAAGAACTTATACTAAACTACCAAGCTTATT2700.11058005381562619No Hit
CAAAGTGGGCGTCACTTTTATGGCGTTAATTTCCTGCCACTACAGATCTTTT2690.1101704980607535No Hit
TAACTGGGATTGCTCTTAAACTTGGTGTGACTGCTAATGATGTAAAGAATGT2690.1101704980607535No Hit
GCAATTGGGGAGACCGAGCTGCAGAAAGACGGGAGAAGTACGGCATTCCAGA2690.1101704980607535No Hit
GATAGCGGGGTCAAGATTGGAAGTGGAACCTAAGTGTCAAGAGTTGACAGTC2680.10976094230588081No Hit
TGCAACCAGCTACTCAAAAGCTTCGACTTCCACTTTGGCTTCTGCATCCCCA2680.10976094230588081No Hit
AAAGCGGGGCGATCTGTTGACGGGCAAGGACCAAAATGTCAAATTTAGGAAA2670.10935138655100814No Hit
TTAGCAAGGAGTGACAGAAGTAGAGAAGAGAATGGAAAGCAGCACACTCCGT2670.10935138655100814No Hit
TCACCAGGGAAGACTGCAGAATTTGTCCAACTTGACATTCAGATTCTGCTTC2670.10935138655100814No Hit
GATAATGGGTTTCTTCTTGCATTTCAAGATTGCTTTTGTTTTGGGGGGACAC2660.10894183079613542No Hit
ATGTATGGGGAAATTGTTACATGCTCACATGTTGGACATGTGTTTCGGAAAG2660.10894183079613542No Hit
GCTTTAGGGATCATCCACTTTTAATGTTTTCCATCCACATAGCTGCATTGCC2660.10894183079613542No Hit
CAATCGGGGAGGGTTCCCATTTGCACTTCATCTTTCAGTAAAGTCTTGTCAG2650.10853227504126273No Hit
TTACATGGGGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT2650.10853227504126273No Hit
AATACAGGGAGGAGGAGGAGGCCCGGCGGGCAGCTGCAGCTTCTGCTGCTGA2650.10853227504126273No Hit
TGATTTGGGTCTATAGCTGGGATTAAAATGCCACTTGGGTGGAGTGGATAGT2640.10812271928639006No Hit
ACTCGCGGGCAGACTCTAAACACACAAGTAGATTGATCTAAGGCATGCTCCC2640.10812271928639006No Hit
TTGCTACGGGGCCCCTATAATGATTGGAAGCATTCCAATGACTGGAATTACA2640.10812271928639006No Hit
GGCTCTGGGTTGCTGCTTTCCCAGTGCCAGGAGAACCCCCGCTCCGAGTCAG2630.10771316353151736No Hit
TCGAATGGGAGCTTGGAAGCTTAAAGTCCAATCCTTACTTCGAGTTTGTTTT2630.10771316353151736No Hit
GCACCTGGGGAGTTGAATCAAATTTTACTCACTATGAAAAGTACAACTGTCA2620.10730360777664467No Hit
GTTATAGGGCGATCTTATGTGGGGTAGTTGGAGAGGCATGGTTCTGTTTGTT2610.10689405202177198No Hit
GCACTGCGGGGGGGGCATATTGTAGTCCTGTCATTTAAGTTATGTAAAAAAT2610.10689405202177198No Hit
TTCTATGGGATTTCTGGCTAGTGTCTGTTAATAAAACACACATACTTAACTT2600.1064844962668993No Hit
AATTATGGGTATGTGTGTATAACTATAAGTAAAATGCAAAGGCATCTGTGTA2590.10607494051202661No Hit
TCTTTAGGGGATTTTTCCATGTGTTTCTGCATATTTCCACATTCTTCCTTCT2580.10566538475715391No Hit
TAGTCTGGGGGCACAGAGCCACTTGAAATATTTACACTGTTACTAAAAGCTG2580.10566538475715391No Hit
ATCTAAGGGAACATTCTGCATGCTATTTTGCTCTTTAGTAGCATAATTGTAA2570.10525582900228123No Hit
TATCTAGGGGATATCTTACCTACATAAACTATTAATATTTTGAAGACAAATG2570.10525582900228123No Hit
CTCTCGGGGTGAGTGGGGGAGGATCCAGCTTCCTTCTGAAAAGGCGATCTTC2570.10525582900228123No Hit
ATCAAAGGGCAAGCAAGAAATCAAGGTAATGTGGAGTTGGATATTAGCAGTG2570.10525582900228123No Hit
TGATGTGGGGTACAATTAATTGTGTTCTTGTGACCTGATGATTTTTTGAAAA2560.10484627324740853No Hit
TTTGTGGGGAGTGCGAGATCCGCTGCTGCTGAGGAGAGGAGCGTCAACAGCA2560.10484627324740853No Hit
GGTCTCAAACTCCTGACCTCAGGCGATCCACCGGCCTTGGCCTCCCAAGGTG2550.10443671749253586No Hit
ATTTACGGGATCTCACGAGCAGAGTCTCAGAGCCGCCAGAGGCCACCCCTGG2550.10443671749253586No Hit
TATGGTGGGACATTTTGTTATGTGTATTTTGCCCCAATAAAAAAATAGAAAA2550.10443671749253586No Hit
ACGGCTTGGGGGCGCGCTAGGCGGAGCGAGGCGCCCGCCGCCGCCGAGCCGA2540.10402716173766316No Hit
TAAAAAGGGACAATAAATGAGTTTCAATAACATTAGGAGGCAATTGAAGACA2540.10402716173766316No Hit
AGTTACGGGAAGCTGCTCATCATGAACTGGCTGTTGTATGATCACCAAGCCA2530.10361760598279048No Hit
CCCTATGGGAGTGGGAGAAGTCATCTAAGTCATCTCCCGTTCCAAAGATGAG2530.10361760598279048No Hit
AGATTGGGTCCCCTCACTAAGGGAATCAGAATCTCAATAATCAGTTTGACAA2520.10320805022791778No Hit
CTACGGGGGATGTAAATGGAGGATGTGTGGGTAGCTTGTATATCATCAATTT2520.10320805022791778No Hit
GACTAAGGGGTTCTTTTTGCAAATAAAGTGCTCATTTTGGCTGTTTTCCAGA2520.10320805022791778No Hit
ATCACTGGGATTGTGATTTTGATTTGCATTTCCCTGATGTCCAATAATGTTG2520.10320805022791778No Hit
GTAAAGGGGGCTCTGCGGAGACCAGGAGTCAGACTGTAGGACGACCTCGGGT2520.10320805022791778No Hit
AGGTACGGGGCACCAATAGACTTGCTCAAAACAGGGTTGCTGCAAACCTTCA2510.10279849447304508No Hit
CATAGCGGGCTTCTTGGCTAATCTCTGTACTTCTGCCTTTTAATTACTGGGC2510.10279849447304508No Hit
ATGACAGGCACCTCACAGTGTCCATACAAATTAGTTCCAGAGGGAAGATATA2510.10279849447304508No Hit
AAATCTGGGGTGAGCGGGGGCCACAGCCCGGGCTGGGGGGAGCCGGGGTGCA2500.1023889387181724No Hit
CATTTTGGGGAGCTGTAGAATTAAGGTTTGTATGGCATGATAAGTATAAACC2500.1023889387181724No Hit
CAGGAAGGGCAGTTACACAATGGGCTTTGAAAAAATCCAGGTTTTCCAACAG2500.1023889387181724No Hit
TCACGAGGGAAGAAACAATTGTTTTACTGTACATGTGAATAACAAGAAATGG2500.1023889387181724No Hit
TGGCATGGGGTTCCTTAAATAGGGACCCGTATGAATGGCTCCACGAGGGTTC2500.1023889387181724No Hit
GGCTCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2480.10156982720842703No Hit
TGAAATGGGGACCTGCTTCTCTTCATCACCCAATTGTGAATCTTACCTCTTG2480.10156982720842703No Hit
AGTTTGGGGCAGATATTTAAACATCTTGTTTTATTGAGTATAAAAACACATC2480.10156982720842703No Hit
GTAACTGGGCTCTAGGCAGAGAAGAGGCGATGGCGGCGATGGCATCTCTCGG2470.10116027145355433No Hit
TATCGCGGGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTTC2470.10116027145355433No Hit
GAACTAGGGAAGCTAATTACTGTTGCAGAATGCCCTCGATATAATTATTTCT2460.10075071569868164No Hit
AATTAGGGGAATATTTCAAAGGTAAAAATTATTTCTTCATATTCTAGTTTAT2460.10075071569868164No Hit
GCGTGGGTACCTGCCAATGTCATCCTAAGGTACATGTGGATACCTAGGTTGT2460.10075071569868164No Hit
AGCACTGGTGGAATAAAAAACCTGTATATTTTACTTTGTTGCAGATAGTCTT2460.10075071569868164No Hit
CTTTAGGGGTGTCTTATTTAAGATGCTGATTGTTTGGGTCACTTCATTATGT2450.10034115994380895No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)