FastQCFastQC Report
Sat 31 Dec 2022
SRR5845526.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845526.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences240462
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGC10190.4237675807404081No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGCCGTCT8520.35431793796940886No Hit
AGCAATGGGAAATGAAATTAGAACTTCTGAAATTTGGGCTTGCTATGTAAAT3320.13806755329324386No Hit
CAGCGCGGGCTTATGCAGGGGGAATTTAAGAATAGGATTCATGGATTTCAAC3190.13266129367633972No Hit
TAACATGGGTGTATGACATCATACGAGACAATAAACAACATGAAAAGAATGT3170.13182956142758523No Hit
TGTACCGGGATCGGGGGATCTGGTTGAATCTGAGATTTTGACTGGCTGGTTT3110.12933436468132178No Hit
CTCAGTGGGGTGTCCAGGATTTTTAGCTGTATTCAGTGGGAGAATCAGAGTG3100.12891849855694457No Hit
GTTGTTGGGGTAGGAAGGCTGAAAAATGACGCCATGACAAATGAAATTGTCA3080.12808676630819005No Hit
TTTATGGGATGTGTATGTGTGGAGTATGTGTGTTTGCTTCTCCGTGGATGAA2970.12351223894004043No Hit
AAACCTGGGCTTTTGAGTGCCAAAAAGAGATAGATGGGTATCAGATTATAAG2930.12184877444253146No Hit
TTTGGGGGCTCTTCCTCGCGGAAGTGGGGAGGAGGCGGTTGCGGTTAGTGGA2920.12143290831815423No Hit
ATCAAAGGGAACTGCTATGACGGTTAAGAATGTCAGTATACAAGAAGGAATA2920.12143290831815423No Hit
GACCTCGGGGTCTGAAATGACAAGGGCAAGAGTGAGATAGGAAACTGTGTGT2920.12143290831815423No Hit
TCTTTAGGGGATTTTTCCATGTGTTTCTGCATATTTCCACATTCTTCCTTCT2900.12060117606939975No Hit
AAGCCTGGACTGCATAGCAAGATCTTATTTGTACAAAAAAATGTAAAACTTA2900.12060117606939975No Hit
TTACCTGGGGCAGAATTTAGTGTTATCAGTGATCTGGATTTTGGTATATAGT2890.12018530994502251No Hit
ATGTATGGGGAAATTGTTACATGCTCACATGTTGGACATGTGTTTCGGAAAG2860.11893771157189077No Hit
TTATGGGGGCAAAATTCCAATGGCATTTCCTAAGGAAATAGAAAAACTAATC2840.1181059793231363No Hit
CCACTTGGGATCCCATGTAAATGAGAAAGGGTAGAAATAGGCAAATTTGTTT2830.11769011319875905No Hit
TGGTGAGGGGGCGCAATAGATATAGTACCGCAAGGGAAAGATGAAAAATTAT2830.11769011319875905No Hit
TGACTGGGGGAGGAAGGGAGCTGAGGAAAGGAAGCAGGAGAAATGATTCCAC2830.11769011319875905No Hit
TTCTATGGGATTTCTGGCTAGTGTCTGTTAATAAAACACACATACTTAACTT2820.1172742470743818No Hit
TTATTGGGGCCTGGAAGAACTCAGAGGGGATGAAGGCACGAGGAGTCAGAGG2810.11685838095000457No Hit
CGTCAAGGGGATGTAATGAAATTTCTCTGTAATTCTAAACAACAAACATCAA2800.11644251482562733No Hit
TGATTTGGGTCTATAGCTGGGATTAAAATGCCACTTGGGTGGAGTGGATAGT2780.11561078257687285No Hit
ATCACTGGGATTGTGATTTTGATTTGCATTTCCCTGATGTCCAATAATGTTG2780.11561078257687285No Hit
CACACACGGGAGCTGGGGGGAGAAGAACTTATACTAAACTACCAAGCTTATT2780.11561078257687285No Hit
AAAGCGGGGCGATCTGTTGACGGGCAAGGACCAAAATGTCAAATTTAGGAAA2780.11561078257687285No Hit
AGTTTGGGGCAGATATTTAAACATCTTGTTTTATTGAGTATAAAAACACATC2770.11519491645249562No Hit
GGTCTCAAACTCCTGACCTCAGGCGATCCACCGGCCTTGGCCTCCCAAGGTG2740.1139473180793639No Hit
ATGTGCGGGTCAGTCGGTCGGCGGCGGCAGCGGAGGAGGAGGAGGAGGAGGA2730.11353145195498665No Hit
GAATTAGGGGGGCAGCGTCTATATCACCTTGAAGAAGTATGACGGTCGAACC2730.11353145195498665No Hit
CCTGCAGGGAAGCTGCAGTGTATCAAAAGATGTGCCCAATGTTCCCATGAGC2730.11353145195498665No Hit
CGAACTGGGGGAGGAAATAATTTCAGATAAGGTGGAAATAGCAGGAGAACTA2730.11353145195498665No Hit
TTACATGGGGTAACATGAAAACATTCTCCTCCGCATAAGCCTGCGTCAGATT2710.11269971970623217No Hit
CTACGGGGGATGTAAATGGAGGATGTGTGGGTAGCTTGTATATCATCAATTT2710.11269971970623217No Hit
CCAACAGGGATAAAGACAATTATGAACTGGATGAAGACACAGACTAAAAAGG2710.11269971970623217No Hit
CAGGAAGGGCAGTTACACAATGGGCTTTGAAAAAATCCAGGTTTTCCAACAG2700.11228385358185494No Hit
TAACCAGGGGTACTGTGGATGAGTATCTGCAAGACCAGGTAATGACACATTT2700.11228385358185494No Hit
GCACTGCGGGGGGGGCATATTGTAGTCCTGTCATTTAAGTTATGTAAAAAAT2700.11228385358185494No Hit
AAATACGGGGGTCCCTGGGAAAAAGATGAGGGCTGCTTCAGGCTGGTCTCTC2690.1118679874574777No Hit
CAGACAGGGAGTGATCCTCCCACTTCAGCCTCGGAAAGTGCTGGGATTACAG2690.1118679874574777No Hit
GTCACTGGGACTGAAACAACGATTACGAAATGGCCCGATGAATGTTGATGTA2690.1118679874574777No Hit
TATACTAGGGAATTTATCAGTGTTTTCCTTTTATAATTTGTACTTTGTGTCT2690.1118679874574777No Hit
ATTTACGGGATCTCACGAGCAGAGTCTCAGAGCCGCCAGAGGCCACCCCTGG2680.11145212133310044No Hit
AGCTTAGGGCAGAACCACACCCCTCGTACCAATGCTGCCATCTCGTTCAGGC2670.1110362552087232No Hit
TTTAGGGGGAACTACTCCCTAGGCAGACAGACTAAAGCTTAGGCCAGCCATG2670.1110362552087232No Hit
CTTCCCGTCCTCTTGATAAAAACAAAATCATCTTTCTGCGTAGGCCACTTCA2670.1110362552087232No Hit
CTCTGGGGGGAGAGCCGCAGCGGCAGCGGCGCGTCCTGCCTGCAGAGAGCCA2670.1110362552087232No Hit
GGCTCTGGGTTGCTGCTTTCCCAGTGCCAGGAGAACCCCCGCTCCGAGTCAG2670.1110362552087232No Hit
GCAATTGGGGAGACCGAGCTGCAGAAAGACGGGAGAAGTACGGCATTCCAGA2670.1110362552087232No Hit
AAATCTGGGGTGAGCGGGGGCCACAGCCCGGGCTGGGGGGAGCCGGGGTGCA2670.1110362552087232No Hit
GTTTGCGGGGAAGAGACCAATTAAGATTCATTCCTCATTCTGTTTCTTTCCA2670.1110362552087232No Hit
GATAGCGGGGTCAAGATTGGAAGTGGAACCTAAGTGTCAAGAGTTGACAGTC2660.11062038908434596No Hit
GCACCCGAATCACTCGAAGCCAGCCCAACCACACACCTGCAGGCCCGCCTGG2660.11062038908434596No Hit
AGTTACGGGAAGCTGCTCATCATGAACTGGCTGTTGTATGATCACCAAGCCA2640.10978865683559148No Hit
GACAAGGGGAGGACCGAGTGGCCCCGCCAGGATTAACACAGATTCCTCAGAT2640.10978865683559148No Hit
ACACATGGGATATTTTATTACTTGTAATTAATATTACTGTTCAAAATTTAGG2640.10978865683559148No Hit
AGTTTAGGGGGGCTGAGTGCACAGGTGGTACTTAATAATGTAGACTAGATAG2640.10978865683559148No Hit
TATCGCGGGAGTGTCAGGCTCTGAATCTCTCAAAATGTGCCGTCTTTGGTTC2620.108956924586837No Hit
TACATTGGGAACTTAAATTGATACAATAAAAAAGAGAAACATCATTAGAACA2620.108956924586837No Hit
ATTATAGGGGAAGAAAAGACAATAGAAGGTTACCCTGGAAAAACAACACCTA2610.10854105846245976No Hit
ACATAGGGACTAATGGAGTAGATATGTGGCATTCAACAAAGAGAGGTTCCAG2610.10854105846245976No Hit
GAGGATGGGAATTGTGAATAGAGACCCCAACAGATATTAACCACCAATGTTC2610.10854105846245976No Hit
GCGAAAGGGGAGATAGTCTTAAAAATTAGCAGGATAGCAACCACTGGAGAAG2600.10812519233808253No Hit
TGAAATGGGGACCTGCTTCTCTTCATCACCCAATTGTGAATCTTACCTCTTG2600.10812519233808253No Hit
TTGCCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2600.10812519233808253No Hit
CAGGTTGGGATGAGTGTGGGCTATGGAAAGACAGCTTTTCCTACACTGATAA2590.10770932621370528No Hit
GGCTCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2590.10770932621370528No Hit
TTAGCAAGGAGTGACAGAAGTAGAGAAGAGAATGGAAAGCAGCACACTCCGT2590.10770932621370528No Hit
AACGTTGGGCAGATGTCTTATGTCTCCAGAAAATTCAGATCCACAATTCCAC2580.10729346008932804No Hit
CAAAGTGGGCGTCACTTTTATGGCGTTAATTTCCTGCCACTACAGATCTTTT2580.10729346008932804No Hit
GTTATAGGGCGATCTTATGTGGGGTAGTTGGAGAGGCATGGTTCTGTTTGTT2580.10729346008932804No Hit
GATAGTGGGATCACTGGCTAATGAGAAAAGGAGACAGCTAACTCTAGGATGA2570.1068775939649508No Hit
ATCAACGGGGAAGATTTCTAAACAGCTCCACCTTTGTTACAATATTGTGAAA2560.10646172784057356No Hit
TGGCTGGGGCGACCTCACTGTCAGCGTTTCTCCACCTGAAGTGATGATGGAT2560.10646172784057356No Hit
TCTCGAGGGAGTCTACCACCAGTGGCTAATGCTAGCTATGAGCCAGGCACTA2560.10646172784057356No Hit
CGTTTGGGGAGTTCCAAAGAGTAAACTCACAGAAGTCTTGAGGTTTCAAATA2550.10604586171619633No Hit
TAAAAAGGGACAATAAATGAGTTTCAATAACATTAGGAGGCAATTGAAGACA2550.10604586171619633No Hit
GCTTTAGGGATCATCCACTTTTAATGTTTTCCATCCACATAGCTGCATTGCC2530.10521412946744185No Hit
GCACAGGGGAGACTCACAGACTTAAAGTAGAAGCAAAACAAACATGATGGCA2530.10521412946744185No Hit
ACCGGCGGGTACTTTTATCTGAGAATTCCTTTTGATATCTGACACCCATTTT2530.10521412946744185No Hit
AATTATGGGTATGTGTGTATAACTATAAGTAAAATGCAAAGGCATCTGTGTA2530.10521412946744185No Hit
AGGTACGGGGCACCAATAGACTTGCTCAAAACAGGGTTGCTGCAAACCTTCA2520.10479826334306461No Hit
GCAGGCAGGGAACCTTAAATTGATACTGTTGTTAAAGAGGGCTCAGTGCATT2520.10479826334306461No Hit
AGAACTGGGGTTAGCGTAACTCTAGAGAAGGAAGGATCAAAGAAGCTTAAAC2510.10438239721868736No Hit
TGTGGGGGGGTCACTAGGTCACGGGGGCCTCACTTCATTCTCTTCCACGTCA2510.10438239721868736No Hit
TGTGGTTGGGCGAAAAGACACAACCTTCTTGGAATCTGACTGTGATGAACAG2510.10438239721868736No Hit
TGCAACCAGCTACTCAAAAGCTTCGACTTCCACTTTGGCTTCTGCATCCCCA2510.10438239721868736No Hit
ATCAAAGGGCAAGCAAGAAATCAAGGTAATGTGGAGTTGGATATTAGCAGTG2510.10438239721868736No Hit
TTCTCAGGGCATTTGCCCACTTCTATGAATTTTATGTATAAAATTTTTTAAG2510.10438239721868736No Hit
AATTAGGGGAATATTTCAAAGGTAAAAATTATTTCTTCATATTCTAGTTTAT2500.10396653109431013No Hit
TATCTAGGGGATATCTTACCTACATAAACTATTAATATTTTGAAGACAAATG2500.10396653109431013No Hit
TTCAAGGGAATGAACTTAAGTCTGTGAATCAGCCTTTGTGTTAACGACTGGT2500.10396653109431013No Hit
GTGCAAGGGGGCTAATTTTATCAAGTCTAGTTTAATCTCATATTTACAGAGT2490.10355066496993288No Hit
CATTTTGGGGAGCTGTAGAATTAAGGTTTGTATGGCATGATAAGTATAAACC2480.10313479884555563No Hit
TTAGTAGGGATCCAGCCTAATCTGGAAAACAGTAACAGCAGCAGTGAACTAA2480.10313479884555563No Hit
GCACAGGCCTATAAAAGGACTTGTTTTGATTTTTTTAGAACAAAACTTCTAT2470.1027189327211784No Hit
CTTGTCGGGGATCAAACAAACCTGTGAAAAATTTATGGTGAAAATATTACAT2470.1027189327211784No Hit
TCACGAGGGAAGAAACAATTGTTTTACTGTACATGTGAATAACAAGAAATGG2460.10230306659680115No Hit
TAACTGGGATTGCTCTTAAACTTGGTGTGACTGCTAATGATGTAAAGAATGT2450.10188720047242392No Hit
GTAACTGGGCTCTAGGCAGAGAAGAGGCGATGGCGGCGATGGCATCTCTCGG2450.10188720047242392No Hit
TGATGTGGGGTACAATTAATTGTGTTCTTGTGACCTGATGATTTTTTGAAAA2450.10188720047242392No Hit
TAATGCGGGGACTGTGGGAAAGGCCTCAAGACAAACTCAGACTGTTTTTTTT2450.10188720047242392No Hit
CTTCAAGGGATTTTTCATCCCTGGAATTTGTCTTTGAATGCATCTTATTACA2450.10188720047242392No Hit
TAAGCTGGGCCGATCTGTGGTGGGGATAGATAGAAAATGTTACATATTTTGA2450.10188720047242392No Hit
GCACCGGGATACCATCAGAGACATCATTCACAAGTAACTGATGTATTGGCAT2440.10147133434804667No Hit
TTGCTACGGGGCCCCTATAATGATTGGAAGCATTCCAATGACTGGAATTACA2440.10147133434804667No Hit
TGGCATGGGGTTCCTTAAATAGGGACCCGTATGAATGGCTCCACGAGGGTTC2440.10147133434804667No Hit
ATCACGGGGATTGCACATTTTTCAATTTGATGTTGTGCACGTCCTTTTCAGA2430.10105546822366943No Hit
CCCTATGGGAGTGGGAGAAGTCATCTAAGTCATCTCCCGTTCCAAAGATGAG2430.10105546822366943No Hit
CACAGAAGGGCGCGTTCGGTGATGCTGCATTACCGCTCAGACCTGCTCCAGA2430.10105546822366943No Hit
GATAATGGGTTTCTTCTTGCATTTCAAGATTGCTTTTGTTTTGGGGGGACAC2420.1006396020992922No Hit
GAGTTGGGATTCCCCCACCTGGAAAACAGTGTTATGGCAATGGGTGCCTGGT2420.1006396020992922No Hit
AATCACGGGTCTTTCGGCCGCGCTGGTGAACAGGACCCGTCGCCATGGGCCG2420.1006396020992922No Hit
TATTGCGGGATAAAATTAATGGCTGTCATGTTCACTCTGAAAAAAATCTAAA2420.1006396020992922No Hit
GTCACAGGGGTCTGGCTTGCACTGAATGCCCAGAAATGGAAGCGCAAGGAAA2410.10022373597491495No Hit
CAATGCGGGGTGGTGAACATGGAGGTGTCCACAGTAAGCGGGAGAGAGGCAG2410.10022373597491495No Hit
TTGTATGGGGAAGAATTCTAGTTTGTTGAGTGGATTCAGAGGAGGTTCTAGC2410.10022373597491495No Hit
ACCAGTGGGCATCTTTGTTTGAAACATCGCAGGATAATGATACTTATTGAAA2410.10022373597491495No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)