FastQCFastQC Report
Sat 31 Dec 2022
SRR5845521.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845521.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences310648
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGC7720.2485127861759934No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGCCGTCT5370.17286446395920785No Hit
AAATGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA4090.13166027143261827No Hit
AGCGGCGGGGATGCAGTCAACAATGAGGTACTACTGAGTATGGAAGTTATAG3500.11266771393989337No Hit
AGAGGGGAACTATCAACATTTTAAACCAAATTGCCTTATTTTTCTTCCAAAC3200.10301048131647396No Hit
AAGGACTGGGAACATCTTAAGAAATATTAGACTGCAAACTTAAAAATCTGTC3170.10204475805413202No Hit
TCAGTGGGAGAGTAATGTATTTATTTCATGAGTTAACCCCCTGTTAACAGGA3170.10204475805413202No Hit
AACGATGGGACTCATTTAGAGGGATCGCTCAGGCCGCAGCAGGAATGAATGG3160.10172285030001804No Hit
TGTTGTGGGCAGAAGAAGGTTATAGAACAGCAAACAGATTTATCCAAAGAGA3140.10107903479179006No Hit
TTTTGGGAGGATGCTGTTGTATGCTCTTTTTGTTTGATACAGAAGCTCAGAA3110.10011331152944812No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)