FastQCFastQC Report
Sat 31 Dec 2022
SRR5845509.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845509.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences132845
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGCCGTCT4510.3394933945575671No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGC3890.29282246226805675No Hit
GTGTAAGGGATTGCTCATGTAATGGGACTTCAGGGACAGCAGAGCAGGCAAC2710.20399713952350484No Hit
GTGAAAGGGGAATCACCACCAAAAGAATAAATCATCTAGGAATACAGCTAAC2250.16937031879257783No Hit
AACTACGGGATTACAGCTCATGTACGTCTGTTTTTATAAGATCAATATTAAA2000.15055139448229138No Hit
TCACTGGGGTAATACAACTACTTAAAAAATATAGTCAATAGGTTACTAAGAT1960.14754036659264555No Hit
GACCAAGGGCTAGTCCTAGAAAATAAACCAAGGCCATATCAAATAAGAAATC1900.14302382475817682No Hit
TACTTTGGGGTTATGTTTGCATATTAATGTCTATTACCTATTTATTTAATGT1900.14302382475817682No Hit
TTGCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1800.13549625503406226No Hit
TTGTTGGGGCAGGAATACAAGTCCATTCACTATTCAAATGGCGTCTTGCTTT1790.13474349806165078No Hit
GCCCAGGGTGCTGGGATTGCAGGTGTGAGCCACTGCGCCCGACAACAAGACT1780.13399074108923933No Hit
CTAGATGGGGATTTGTAATTTTCCTTTTTTATATTGACTTTGGTTTGATATT1770.13323798411682788No Hit
ATTCTTGGGGGATGTAGAATTAGGGTGTGGTATGGTGAGGGAAAAAGGCATT1750.13173247017200496No Hit
TCATAGGGGAGCAGGAATCAATGAAATTGATAACAGAAAAAAGTAGAGAAAA1730.13022695622718206No Hit
GGCTGGGACAAGCTGGAATGTTCTCCCATGGTTGTTTTTGGGGAAAACTGGA1710.12872144228235913No Hit
ACTGGTGGGGGTTATAGAAAAAGTCAAAAGGAAAATAAAGATATATTTTGAG1710.12872144228235913No Hit
TATTCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1690.12721592833753623No Hit
CACTTTGGGTATTGCACTTCCTGTGAAGCAGGAGCCTCCACAAATTGACGCA1670.12571041439271333No Hit
CCTATTGGGCAGTTTTGAGAAGTCATATAGAAGGCATATATTCCCTTGGTAA1650.12420490044789041No Hit
AACTAAGGGGAAGCTGTTCAGAAAAAAGTTAACATTGCAGTCCTGAGACTGC1650.12420490044789041No Hit
GATGCAGGGAAGTTTTAGTTAAACATAGAAAATATCTGCCCATTATCATCAA1650.12420490044789041No Hit
CTTCGAGGGGGGTGGCACCTCCAGAGTGAAGAAGATCCTTTGTCAAGAAGGG1650.12420490044789041No Hit
GGCAGGCTGGGCTGACATGCATGGTTTAACTTCCTCATCAGAACTCTGCCCT1630.1226993865030675No Hit
CTTAAAGGGCTACCTGCTGTTTTCTGATGACCTCTGGGATCTTTTCATTTAG1630.1226993865030675No Hit
TTCACCGGGACCACACGAGGCTGAGGCACCCCTGTGTATTCAGGATGGGAAA1620.12194662953065602No Hit
GTTTGGGGAGTGAGCAGGAGTAGCTATTCCTATATTAGACAAAACAAACTTT1620.12194662953065602No Hit
TTCATAGGGCTTTTTATTCTTTTTTCTCTAAACTTCCCTTCTCACTTCATTT1610.12119387255824457No Hit
GATTAGGGGGTTGCTGTTTTAGGCCACCCCAGCTCAGCCAATGATTCCTTTC1590.11968835861342166No Hit
TAGGGCAGGCGGAGCTTGAGGAAACCGCAGATAAGTTTTTTTCTCTTTGAAA1590.11968835861342166No Hit
AGCATCGGGCAATTTAGGAATGCTCAATGTGCTGTTGATTCTTTCAATCCAC1590.11968835861342166No Hit
AGACATGGGGATGCCTGAGGTAGAAATAGTAGAGTTTTGGCTGCAAAATGCT1580.11893560164101019No Hit
GATTTTGGGTAGGTACAATATAATCACAAGAGTCCCCGCATGAGGGAAGCAG1580.11893560164101019No Hit
AATTGCGGGAAGAACAAAGACACAACATAGCAGTATCTCTGGGACACATTTA1580.11893560164101019No Hit
TGTTCTGGGCATTTCACGAAGTTTGATGTCTTGTTCTTCCATTGTTTCATCT1550.11667733072377583No Hit
TTGTAAGGGTTAAGGCTGTTTTTTAAAATGTTTATTTGAAAGACAATTTTCT1550.11667733072377583No Hit
AGCATAGGGCTCAAAGAAAACAATAAACAGAAGAACTAGCACATGTGTGTCT1550.11667733072377583No Hit
GTCAATGGGTATAACATTGTGTAGACAATTAGTGGTACCAAGCCAGTCTGAC1520.11441905980654145No Hit
TATAGTGGGATGGGTCTGGGATGTAAAATTGAATGAAATAGAATCTCTGAAT1520.11441905980654145No Hit
GGTCGGGCACAGTCAATAACGAAATACAGCTGGGCTTAGAAGAGTTGTGGGA1520.11441905980654145No Hit
AACTAAGGGCACAAGAGTCCCCGCATGAGGGAAGCAGGAAGGTCAGAACCTG1520.11441905980654145No Hit
TCAGGAGGGGGACATGCTGGCCCTGCAGATCATCGACCTCTTCAAGAACATC1510.11366630283413No Hit
CACGATGGGCGATCTTGTAGCGGATTGTGGTCATGATATAAAAAGAAGCTAA1510.11366630283413No Hit
GATTATGGGCTAATCTGTAGCTTTCTGGGGTTATCTGAATTGTTTCCAGGGC1510.11366630283413No Hit
TTTAGGAGGGCCAGGAAAGTACATTTGCATTAAAAAGTGCAACGGACAGAAA1510.11366630283413No Hit
GTTATGGGGACTCGTGCACATGAAAGGGAGTTTGGTTCTGTAGATGATGAAA1500.11291354586171855No Hit
AGTTTAGGGATTCTACAAGAATGTTTCCTTTTACTTAAGTTAACAAAAGAAA1500.11291354586171855No Hit
TCTAGTGGGCGGGGCCGGGTGTTCCATAGCATGTGTGTCTCGGATGCGGAAG1500.11291354586171855No Hit
TCACCCGGGCTGGCCACATCAATGTTCTCTGACAGAGGTTGAAGGAGTCATG1500.11291354586171855No Hit
GTCCAAGGGTAAAGCCATTTCTCCTCATTGTACTTTTCAAGAATTGTTTATG1490.11216078888930708No Hit
AATTTGGGGATTGAATCCAACTAGGCACTAATGAAAGGCTGAAAGTCCTTCT1470.11065527494448417No Hit
GACCACGGGATCTATGCAAACTTAAGATGGGCGGGATTGTTGTAAAAGCTAT1460.10990251797207272No Hit
TAAAAGGGGTCGCAGCCTGCTTTTCTTAGATGTTTTCTTGCTGTTCCTTGTC1450.10914976099966126No Hit
ACACATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1450.10914976099966126No Hit
TCTTGGTGCGGCAGAGAATCTCTTCTGACTTTGAAAAGATGAGATCTGCTCC1450.10914976099966126No Hit
TCCAATGGGATGATGACCTCAGGATATTTAGGAATTTTTACTAATTGTTTCA1450.10914976099966126No Hit
TGAAAAGGGACGGAAAGAGAAGGGTTCACGGGAAGAGAAGCAGAAGCCCCAG1450.10914976099966126No Hit
ACTGATGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1450.10914976099966126No Hit
GGCAACGGGGCATCTCAAATGTTTTCTGCAGAAAAAAATAAAAAGATTCTAA1440.1083970040272498No Hit
AGCAGTGAGCCGAGATTTCTTAGAAGGAATTGTTACTTATTGTTCTGAAGTG1440.1083970040272498No Hit
GTCTCAGGATGGAAGAAAAATTATCAAATCCTGTCCTCATGGAGCTTATGTT1440.1083970040272498No Hit
GGTAATGGGCTCCGCACCACCCCACTTCCATTTTGGCCAAACCAACCGCACC1430.10764424705483834No Hit
TAAAGCGGGGCAATAAGCAAAGCAAAATAAAATTTGAAGTTGGGGTGATTTG1430.10764424705483834No Hit
TTAAGAGGGGACACAATTCTTTGGTGTAAAATAATATGTCTGTTATAAATAA1430.10764424705483834No Hit
ATAGTAGGGATCTGTAAGAAGGTACTGGAACTTTCAGGGCTTGAATAACAAT1430.10764424705483834No Hit
GCTTCGGGAGAGGAGGGTTAGTCCAGGTCCTCCTAATTTGTCATCTGTTTCA1430.10764424705483834No Hit
TGCCAGGGGCATTCATTCAAGAAAGAACAATTGGCAAGAAATAATTAAATTA1430.10764424705483834No Hit
ACGAATGGGACCAAGGGTAATGAGAATTAATATTTTCTTCAATGTCTTTTTT1420.10689149008242689No Hit
TGCCCTGGGCTGCTCTAAGCACTTTGCATACGTATTAGCTGATTTATTATTC1410.10613873311001543No Hit
TTAAATGGGCATGGTTGCTGTTCTATTAGAGCCATTTAATCTCATTTGTCTC1410.10613873311001543No Hit
GTTCCGGGGAAACTGGATGACATTATGCTGGATGTTATAGGCCAGAGAAGGA1410.10613873311001543No Hit
ACCTCAGGGATTATCAGCAAGATAAAGACAGATCTTATTATCTGTCCTTAAA1410.10613873311001543No Hit
CTGTTTGGGCAGACCATTTCCTGGTTAGTCAGCACTCAGGTCTTTCTAAGGG1400.10538597613760398No Hit
TAGAACGGGGAAATTTAACATAATGAAACATTTCCTTAAAAAAGAAAAAAGC1400.10538597613760398No Hit
GGTTAACGGGGTGGCACATTATGAATCTACTCAGTCTTTGCTTGGAATTTAT1400.10538597613760398No Hit
TTCATCGGGAGTAAGTAACAGTAATGAGTTCAACATAATTTCTTATATAGGA1390.10463321916519251No Hit
GACTGAGGGGACTTTGGCGATGGTATCGCGGTGTCTTACGTCAATCTCAGCT1390.10463321916519251No Hit
GCCTCTGGGGTAGCCACTGCTGTTTGCCCTGGCCCATCAATGAGAAGATCTG1390.10463321916519251No Hit
TTACACGGGAAGGTCGCTGAGGTGCTGGCCTCAGGCCGTGCCATCCGGAAGC1380.10388046219278106No Hit
TTCTAAGGGAGCTTAAGAAACCTGAGACTATTCCTCTAAAAAGAAGTCTAGG1370.1031277052203696No Hit
AGCCCTGGGATAGAAGCTGAGTCTCTGATCCAATATGTTTTTATTTGTTCCA1370.1031277052203696No Hit
GTATATGGGAAGGACAGCCCCCTTCACCCTCCATCCCTGCAGGCTCAGGGGT1370.1031277052203696No Hit
GTAAACGGGGAGAATTCACACTGGAGAGAAGCCTTTTAAATGTAGTGAATGT1370.1031277052203696No Hit
TATTATGGGTTAGTGGTGAGCTGGTAACAAGGGATAAATTTTTAATTCAAAG1360.10237494824795815No Hit
TTGGGAGGTTGAGATTGCGCCACTGTACTTCAGCCTGGTACTTCTGAACTGT1360.10237494824795815No Hit
CAGCCTGGGTGACAGAGCGTGACTCCGTCTCAAAAACAAAGCACAAAAGAAC1360.10237494824795815No Hit
GTAATGGGGTACACCGATGCCATTTTGTAAGATTGTGAAATGGTTTGCTTTT1360.10237494824795815No Hit
AGGTGTGGGGTTGACTGTGATGTTGGGTAAGTTACTTATCTTTGTACATCAG1360.10237494824795815No Hit
TTAAGCGGGCTAGCAAATTATAATTCTGCTTCAGAGATATGTGATAATTTAA1350.10162219127554668No Hit
TTCAGTGGGGTATCCTTTGAACGTGAAAAGCTTCTTTTGCCAGTAAGATGAA1350.10162219127554668No Hit
GATCGCGGGGTTAATTGGGCAGATTTCAGCACTTGTCTTGCTCATGCTATTC1350.10162219127554668No Hit
TTAGCTGGGAGCAGACAATAGATGGGAGAGGAGTCAGTTTAGATAATTATAA1350.10162219127554668No Hit
GAACTCGGGGTTGGAACCAGATCCTGGCTGAGAGCAGAGACCAGCTTCCACT1350.10162219127554668No Hit
ATAATAGGGCACAAGGAGGCAACCAGCCCCAAGGTCCCCCACCTCCTCCAGG1350.10162219127554668No Hit
GCCAACGGGCAGCAGTTAGCATGTTATGTTTTTCCATTGTCTAACAACTTTA1350.10162219127554668No Hit
AGGTGAGGGATTTCCTCCTTCGATGTTTTCCTCTCAGTTGTAAAAGATGAAC1340.10086943430313523No Hit
AACTATGGGAGGGGCCTTCCAGAGTGTATGGGTTTCGACAGACCATGATGAA1340.10086943430313523No Hit
AAACCTGGGTAGCATTAACCGCCTACATTAAAAAATAAGGGTTAGTTTAAAT1340.10086943430313523No Hit
TATAGCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1340.10086943430313523No Hit
ACATAAGGGGGCCGCTTGGAGGTATTTCCTGCCCACATCATATAAGAAGTTA1330.10011667733072378No Hit
AACTTTGGGTCTTTCTGTATTAAAAGACCAATACAGGAATCAAGAAGAAAGC1330.10011667733072378No Hit
AAGTGGGGGTCACTGAGGGAATTGTGGTTTTACGTGCCATGTATTGGTGTCA1330.10011667733072378No Hit
TCAAGAGGGAATCTTAGCTCACTGCAGCCTCTGACTCCCGGGTTCAAGCAAT1330.10011667733072378No Hit
TTCATTGGGCCTCTGCTTCTGATTTCTTAAATAGTTTTGTTTTATATATTGT1330.10011667733072378No Hit
CTCAGAGGGGTGAGGGGAAGACGCACGTGCAGTGGGGCTGATGGAGGCATCA1330.10011667733072378No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)