FastQCFastQC Report
Sat 31 Dec 2022
SRR5845495.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845495.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264407
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGC7720.2919741156625959No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGCCGTCT6460.24432030922025513No Hit
TAGTAAGGGAGATAAACTGAAAAACTGAAAGATTGTTTCCAAAGGTTCTTAT4350.16451909366998604No Hit
AATCGGGCGTGGACAAGAGGGATAGGAAGAAGAGCATTCAGCAACTAGTTCC3100.11724349204067971No Hit
TCATGAGGGATGCATCAGATGCTGGATCAGACTTTACTGGAGTTAAACAACA2990.11308323909730075No Hit
TTATGTGGGATCACTGTCATTTTAGGTGTATTTTTTTGCAGAGTACAACATG2810.10627555246268065No Hit
GGCCTGGCAAATAATGCTGGGACAACAGGACATCTGCATGTGAAAAAATAAA2730.10324991395840503No Hit
TCTTACGGGCAACCGCTTTGAAAAATAATTCGCTTCTCAATAAGGCCAACAT2720.10287170914537058No Hit
GTTACAGGGATTCCATGGTAATAGCAGTTCTTTGAGTCATTCACTTGTTGAA2720.10287170914537058No Hit
ACGTTGGGGTCTCAACTATATGTGCAATATTTGGTTTCTTTAAAAAAGATCT2660.1006024802671639No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)