FastQCFastQC Report
Sat 31 Dec 2022
SRR5845494.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845494.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences264163
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGC7280.27558742140269454No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGCCGTCT6730.25476694313738113No Hit
TAGTAAGGGAGATAAACTGAAAAACTGAAAGATTGTTTCCAAAGGTTCTTAT4430.16769948857334296No Hit
AATCGGGCGTGGACAAGAGGGATAGGAAGAAGAGCATTCAGCAACTAGTTCC3590.13590093994995514No Hit
ACACACGGGAGATTCATGAAGATCACTGTTATTGGACGTTACGGGAGTACAA2980.11280913678297112No Hit
GGCCTGGCAAATAATGCTGGGACAACAGGACATCTGCATGTGAAAAAATAAA2860.10826648697963No Hit
GTGACCGGATAAGGACAACATGGATGCTAGTTAGAAGACAAAGCACTACTTC2770.10485949962712417No Hit
TATAAAGGGACCATGATTGCTTTATGTTTATATTTAACTTGTATTTTTGTAC2770.10485949962712417No Hit
TTATGTGGGATCACTGTCATTTTAGGTGTATTTTTTTGCAGAGTACAACATG2770.10485949962712417No Hit
TCTGGCAGGGAACTGAGATGTTTCTAGAATGAAACAGTAAATGTGCCTGTAA2740.10372383717628889No Hit
TCATGAGGGATGCATCAGATGCTGGATCAGACTTTACTGGAGTTAAACAACA2720.10296672887573204No Hit
AATTCTGGGCATGAGTTAATTATTTGAGATTGTTTAAATACATTTTTATATC2710.1025881747254536No Hit
TTATAGGGGGACAAATTTTGTGTTGACTTGAAAGGAATCACACCATTATTCC2670.10107395812433989No Hit
CGCAATGGGGCCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCC2660.10069540397406147No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)