FastQCFastQC Report
Sat 31 Dec 2022
SRR5845493.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845493.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences257908
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGC7140.27684290522201715No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGCCGTCT6220.24117127037548272No Hit
TAGTAAGGGAGATAAACTGAAAAACTGAAAGATTGTTTCCAAAGGTTCTTAT4550.17641949842579524No Hit
TCATGAGGGATGCATCAGATGCTGGATCAGACTTTACTGGAGTTAAACAACA3020.117096018735363No Hit
AATCGGGCGTGGACAAGAGGGATAGGAAGAAGAGCATTCAGCAACTAGTTCC2990.11593281325123687No Hit
TATAAAGGGACCATGATTGCTTTATGTTTATATTTAACTTGTATTTTTGTAC2850.11050452099198163No Hit
TCTGGCAGGGAACTGAGATGTTTCTAGAATGAAACAGTAAATGTGCCTGTAA2660.10313755292584953No Hit
TAAGCAGGGGGAGTCAGGCAGGAGAAGGAAATAAAGGGTATTCAATTAGGAA2640.10236208260309879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)