FastQCFastQC Report
Sat 31 Dec 2022
SRR5845480.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845480.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213708
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGC7800.3649839968555225No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGCCGTCT7340.3434592996050686No Hit
GTTTGCGGGTAAAACTTTTTTGTATTATTGTTTGTTTTGTGTTCAGTTCAAA3440.16096730117730734No Hit
ATTGTGGGATGACAAAGACCTCAAAAGCAAATGCAACAGAATAAAAAAATAG3360.15722387556853276No Hit
TTTCTTGGGTGGAGATCCCAAATTTATAAAACAATTACTACTAGACATAAGA3230.15114080895427406No Hit
ACTATTGGGACACACTGACATTGTAATTTAAGTTGATGTATTTTTGATTCTG3200.14973702435098357No Hit
ACTTATGGGATCCAAAATTAATAAGTATCTGTTGGCCTCTGTTTTCTCCCAG3120.145993598742209No Hit
TATCTAGGGCAAGAACTGGAAGGAACTCCCTGCTGTTTTAGAAAAAGAGAAA3110.14552567054111218No Hit
CTAGCGGGGAGATTCAGTCAGTCTTCTATGGTTAATAAAATCTATAGTGAAC3010.14084638853014395No Hit
CCAAATGGGGGAAGGGGCAAGACGGGTTCAGTTCGTCATGGGGCTGTTTGGA2920.13663503472027252No Hit
CATATCGGGGAATCAGTATTCCATAGTATTTGCTTTCCTTGTATCTTCTTGA2900.13569917831807887No Hit
TGCAGTGGGGTCACCAAAACTAGGACATTTTTGAGAGTAAAAAGAGGCAATC2870.1342953937147884No Hit
GAATTGGGGCAAATTAATTGTGTGAACTTGGAAGCACACCAATCTTACTTTG2850.13335953731259476No Hit
TTTATGGGGAAAAATGCATTTTCCAAACAACTTATACATGCTTTTTATGACT2820.1319557527093043No Hit
GAATCCGGGATGTGAATCAACAATGAATAGTCTCATTTTGGACATGGAAAAA2820.1319557527093043No Hit
CTTTGTGGGAATACCATCAGTTACAAACTTTGGGACATTACTTAACCTCTCT2780.130084039904917No Hit
GGTACAGGGCTCTACAGGAACTTAGTTCTTGTATGGCAACTTGAGTGGCATT2770.12961611170382017No Hit
CCAATAGGGAAGACATTTCAGTTAAGCTCATAAAATTTCATTGTTTTCATTT2770.12961611170382017No Hit
GGCGCAGGGAGTGTCTCTTACTGTACATTTTCTCCCTAATTCCTATGGCATT2750.12868025530162652No Hit
TGTGGGCGGGATTCACATAACATAAAAGTAACCATTTTAAAGTGTACAATTC2740.1282123271005297No Hit
GAACCCGGGTGGCAGCTGTGGCGCCGACATGGCTGCGCTGGTGGAGCCGCTG2730.12774439889943287No Hit
ACACTGGGGGACTCAGACTTCTGAGGAATTTGGGTTTGTTCCCCTTTGTAGA2720.12727647069833606No Hit
GCTATAGGGAGAAAGGGAACCAGGAGAAGTTGTGGACAGCCTGGTAGGCAAA2720.12727647069833606No Hit
ATTGCCGGGAGTCCAGTTATGAGTAATGAGATTCAATCAGTAATTAAAAGTC2710.12680854249723922No Hit
TACACCGGGGAGTGGCCGAGCCAAGGAGCTTCTGGGCCAGGGCCTGCTGCTG2700.1263406142961424No Hit
GCTATCGGGCACACACACAGAAGAAACCCTCAGAATACGCTTAACCAAGGAT2690.1258726860950456No Hit
TGAAATGGGGGGGATAAAAAGATTAAGAGATTTAGCTAGTGATACACAAGGA2670.12493682969285194No Hit
TGATGCGGGGATGCAAAATAAAACAACCCTCAGTTCATACCTAGCTCCCTGT2660.12446890149175512No Hit
ACACCAAGTTCCTCCCAGATTAAACTTCCCAGGTGTTCAGAATTACTTTTGC2660.12446890149175512No Hit
AACATCTGGGGATCATAACTAGTAATATGAGTTAATGTTGAATCTTTTTTGA2660.12446890149175512No Hit
TGATGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2650.12400097329065829No Hit
TCCATTGGGAGATTTGAAAAGCTGACTTTTTAACATCTTGAGGCAACTGTAG2640.12353304508956146No Hit
TCTACCGGGTGAAAAAAAGGATGCAAAATAAAAAGCTGCCTAAGGTGAAAGT2620.12259718868736783No Hit
GCTGCATGTTTCCAGAATTTTAAGCTATTATCACTTAAAGGTTATTACACTC2620.12259718868736783No Hit
TATTTGGGGGTACCTTTTTCTGGTAAATTTTGTAGCAGAAATAAAATGACAA2620.12259718868736783No Hit
TAATATGGGATAATAAAAAAGCATTTCTAGCTACAAAATAACACAAGGAACA2610.122129260486271No Hit
TTGAGCGGGCATGTGGATCTAAATTTTAAAAATAGGAATGAATCTCTTAATT2610.122129260486271No Hit
TTGTAAGGGCGATCTATGCGTGGGACAATTAAAATACTCATAGTAAATCATT2610.122129260486271No Hit
ACAAGCGGGTCCTTTAGATTGCTGGAGCATATTCTGTTGCATTTTGTTTATC2600.12166133228517417No Hit
GATGCTATCTTCAGGTACAGATACTCCTCAACTTACAATGGGTTTATCAGGA2590.12119340408407735No Hit
CGTAACGGGATTCCCTGGGATGTTCTATTAATAATAAAGTGATAATAAATGA2580.12072547588298052No Hit
CGGTATGGGCTTACTCGGGAGCTGGCTGAAGATGGCTACTCTGGAGTTGAGG2580.12072547588298052No Hit
GCATGTGGGAGATTACACTGGATGAATTCAAAGAAGCTGCAAAGAGCGACCC2580.12072547588298052No Hit
AAGCCTGGGAAAGAACTGAAATCATAACCAACAGTCTCTTGGACCAGAGCAC2570.12025754768188371No Hit
ATTTACGGGTGCCTCTCGGGCTCTGACAGTGTCATTGACACTGTTGCTGATC2560.11978961948078688No Hit
GTGAACGGAGGGATGAAATTGCACTATAGCCACAGCGTTCTGTTTCTTAAAA2550.11932169127969006No Hit
GAAGGCGGGTCATACAGCTAACTCACCTATCTCATCCATCAAACTTAGTTTC2550.11932169127969006No Hit
ATCACCGGGGAGGACTCTTTGTCTTTACGGAGTTCATTATTCCAAGTGCATG2540.11885376307859323No Hit
CACTTTGGGCTACATAAGTAATCTGCATATTTTTAATGCCTTCATTCATGAA2540.11885376307859323No Hit
ACAGTCGGGTAAATCGAAAAATGTTGATGGCTTTCTTGTGTCTTTATCAAAT2530.1183858348774964No Hit
GGCTCGGGATCCCTTCTTGCCATTCTGTCATCTAAGCGCCCATCTGGCTGTA2530.1183858348774964No Hit
TTTACTGGGATATGTGTTGATTCTTGAAAACTGGGACGTGTATTTCCATTGG2530.1183858348774964No Hit
GAATTTGGGCACACAAACTCCAAGGTGTTTTTAAGAAGACCAGTGAGGGATC2520.11791790667639956No Hit
GTAGGAGGACCACAATCAATGTCTTAGACACAGAGGCAAAGGATGCTTTGGA2510.11744997847530274No Hit
ATCATAGGGGAGGAGATATAATAACATTCTTCTAGCTACATTTGTTGAAAAT2510.11744997847530274No Hit
GACCCAGGCCGTGCACGAGCTCCTCGAGAAGGAGAACATCACCGACCCGCCG2500.11698205027420591No Hit
ACGTAGGGGACTTGCTCTTTTAGAGATGATTGAATTTTATTATTTTTCCCCA2500.11698205027420591No Hit
GCCCAAGGGGTAACATTTCAGATAAATGGACACTTGCCATTTTCAGTTTTAA2490.11651412207310909No Hit
AATGATGGGATTCTAATACAGTAAGATATTGGACACAAAATGGAGGTAACTT2490.11651412207310909No Hit
AATGTTGGGATCTCCCTGGTGAGAATGCAGATGCCCTGGGCTCACAGGGCAA2490.11651412207310909No Hit
TAGTAAGGGCAGATCATTGCTACTCAAAATGTGATCCATGGGCCAGCAGCAT2470.11557826567091545No Hit
ACTTTAGGGAACCACGGAGATAATCAAATTTCTTTGTCAATCATGTTTCTGA2460.11511033746981862No Hit
CAATCAGGGCAACTGAAAACTTAGGTTGTTTCTAAATTAACTATTGTCAAAC2460.11511033746981862No Hit
GCTGATGGGAGCCTTCCAAAGCTCAGGGATTACAGACATGAGCCACAGCACC2450.1146424092687218No Hit
CAATTAGGGAAAGACTAAGGCGTATTATAGGAAGAGGGCTAGAAACCTAACT2450.1146424092687218No Hit
TTCTTTGGGATAGTAGATTTAAATTATTTTAGACTTCTTGTTTGCAGTTCAT2450.1146424092687218No Hit
CGCGATGGGCGATCTGGTCTCGGGGAATTGGATTTTTATAAAAACCTCTGAA2450.1146424092687218No Hit
AACTCCGGGGAACTCCTTCATAAGCAAAATTATTTTTATGTGATCTCTAAGA2440.11417448106762497No Hit
CCTTGGGGCTCTTTCCCTAAGCAGCCTGAGGGTTGACTGGATTGGTGAGGCC2410.1127706964643345No Hit
ATCCATGGGTATATTAGTAATTTATATAGATGTCAGCAATTAGGCAGATCAA2400.11230276826323768No Hit
TAAGTGGGGCGGTAGGAAACACAGTTATTCACAGTGGCCATCCCCAGCAGAA2400.11230276826323768No Hit
GACATCGGGGAAGAAGAAGGGATGATGTGTGGTAGAGAAGATGAGGGTTAAC2390.11183484006214085No Hit
TTCTAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2390.11183484006214085No Hit
TTGGCGGGGCTCAAGTTTATGTGTGTAAACTGAGGCAGAAATGTTAATTATA2390.11183484006214085No Hit
TACTAAGGGGGAAGTAAAAAAAGGTCTAGATAACCTCTACAAGAAAGTTGAT2380.11136691186104403No Hit
GCCCAAAGGGAAGCTGATTGCAGGGAAGATCATCCCAGCCATTGCCACGACC2380.11136691186104403No Hit
CTTGGCGGGGAGGTACATTTCTTTAAGCACAAGAGAAGTAGAAATGGCCTTT2370.11089898365994721No Hit
ACTGCCGGGGGCGCTTAGGCAGGCGGTGGCGCGGCTGGAGTGCCGCGGGGAG2370.11089898365994721No Hit
CTTGCAGGGGTAAATGAACTTTAATCATGGCTGTTAATTGTAAAACATGACT2350.10996312725775356No Hit
ACGCAAGGGACTGTCCGAGCACGAGAACGTGCTGGCTGAGTTCAGCAGCGGG2350.10996312725775356No Hit
GTGCAAGGGCGGCCCGACTCCTCCTGTGTTGAGACTAGTCATCTACTGTTCC2340.10949519905665674No Hit
CTGGTAGGGCTTGCCTTTAAATGCTAGCTCTTATTGAGAGACCTCAGGCCAG2340.10949519905665674No Hit
CAGATGGGAGAAAATGAAATTAATATGAAGTCCAAATAACGATTGCATAAAT2340.10949519905665674No Hit
TAAACTGGGATAGTTGGCATCCAGTAATCAACAATAAATAGGGAAAAAATCA2330.10902727085555991No Hit
GTAGTCGGGTGCAAAAAAATAAATAATGGGAAGAAAGGGCTGTATAATGAAA2330.10902727085555991No Hit
AGCTTAGGGAATCGATATTATTTCTATTATTTTCATTAATTTCATTAAGGGT2330.10902727085555991No Hit
GACTTTGGGATGAATGGCTATTTTAAAAAGTTTTATATTATAAAATTTCATG2320.1085593426544631No Hit
TGCGCGGGAACTCGATGCTGAGTATCTACATGGATACATTAAATATATTTAT2320.1085593426544631No Hit
GCATCTGGGGACCAATATAAGTATAGCTACTTGATATGGTTTGGCTGTGTCC2320.1085593426544631No Hit
TTCTCTGGGTCTCAAGAGGTGAAATACAGAAAGCCTTTTTTTCTTGATCTTT2310.10809141445336627No Hit
GTCTTTGGGGGTCCCTGTGACCGGCTTGGCGGTTGGAGCGGCTGTCGCCATG2310.10809141445336627No Hit
TTTACGGGGCACAAATAGAATAAATACATGAAAACTTGGATTAAGTCAGGCT2310.10809141445336627No Hit
GCTGCTGGGAATTTAGGATGACATACAAGAACAATTCACTTACTAATAATTT2310.10809141445336627No Hit
TAGAACGGGGTAAACTTGAAAGCAAGACCTTGATTGCACCAACAGGTCCAGA2310.10809141445336627No Hit
GGGCCAGGGAACTGGACACACTTGGAAAATGCCTACATCATAAACCTAAAGA2310.10809141445336627No Hit
CTGCAAGGGATACACATTTTCTAGCAGAATTTTTAAAATTTGTAAGTAGCAT2300.10762348625226945No Hit
TGTAATGGGATTATACTTAAAGAAATATTTCATCGTCCAATTATATTTTAAA2300.10762348625226945No Hit
AAACCTGGGATATAGTTCTTCTCACTGTTTCGTAAGATATTTTGATATATTA2300.10762348625226945No Hit
ATACTAGGGCATTTTCCCACTTTTCTATTGAAATATTTTTATTTTTCTAAAT2300.10762348625226945No Hit
ACGAATGGGCAGAGTTTTCAACTCAATATGACACAGATAAATTCACTTCAGT2300.10762348625226945No Hit
TTTAGAGGGCTGGCAAGAAATATGACTTGCTTTTTGCTGAGCTTAATTCAGA2290.10715555805117262No Hit
TCGCGAAGGGGGAGTGAGCAAGCGAGCAGAAAAGAGGTGGAGAGGGGGGGAA2280.1066876298500758No Hit
TACGGTTGGGTAGTGTAAGTGAATCGATGAGTAAATCATTAAACAAATTGAA2270.10621970164897898No Hit
GATTATGGGTTACCCCAAAATACTTGGCTTTGAATGGTTCATCTTGAGTGTG2270.10621970164897898No Hit
TATTATGGGAATGAACTAAGGGTAGGTCCCAAAACTACTGAGTCAGAATCTG2270.10621970164897898No Hit
ATTATTGGGGAAGTAGGTGAACAAAGCCAGAGGACTTACATTTCCAGATACG2260.10575177344788216No Hit
CTCCATGGGGAATTTCTCCATTCATATTTATAACCATTCTAGTTTTATCTTC2260.10575177344788216No Hit
TAAGTGAGGAATTTCTAGAAAGTCAAAGAAATGCAAATTTAAGACAATAAGA2260.10575177344788216No Hit
ATTAGGGGGATATGAATTTTTCTGTATCAATTCCCTCTTCAAGCCTGAATAA2250.10528384524678533No Hit
TGTTACGGGGAACAGAGTCTAGAATTATGATAATCATATGGTTTCCGCGAGA2250.10528384524678533No Hit
AGCGCTGGGCAGATTTTCCTGGTACCCCACAAATAAAGCAAAAATATAGAAG2240.1048159170456885No Hit
CTTATAGGGGAGCTGACCCAAGGGGCCTTCGAGGTGCCTTAGGCCGCTTGCC2240.1048159170456885No Hit
GAGGCACCCCCTATTCCTGCTCCCCCAACTGGATCAGGTAGAACAACAAAAG2240.1048159170456885No Hit
GTGGTTGGGGTGTAGTCATAAATAGACAACCATCATTATTTAAATGGCATAA2240.1048159170456885No Hit
TAGCAGGGTTAGCTATAGTAATATCAGAGAAAATAGACTTTAAGTTAGAGAA2230.10434798884459168No Hit
TTACCTGGGACGAGGTGTGGCTGCAGGAGCAGGGGGAGAACACCTACGTGCC2230.10434798884459168No Hit
AAAGACGGGATTCCTGGTGTGTTTTGGAGGGATAAATCTTGGGAGACCCTTT2230.10434798884459168No Hit
GATATCAGGGAACTTCTTCTGGATATATTTTGTGCAACAGCATTTTAAAATT2230.10434798884459168No Hit
ACTTAGGGGAATGGCTCTTACTAAAAAGTCAAAAAAACAACAGATGTGGGCA2230.10434798884459168No Hit
TAGTTAGGCACCGGTGGACCTCACATTTGCTGCGTTGGTTGCATGTTGCTCT2220.10388006064349486No Hit
AACTGGGAACACACACCAACTGAGACCCAAATAATTTTTCTGCCATTGCTAT2220.10388006064349486No Hit
GTGCATGGGTCTCTTTTAATTTCAGCTATCATTTTTAGAGCCTTACAGTCAC2220.10388006064349486No Hit
TTAGTTGGGAGACTTTGTGGACCATCATCCAATTGTTTTTTGGAACCTGGTA2220.10388006064349486No Hit
ATATGGGGGGTATGTTAGCAATAAGTTTTAAGTGTTTGTTTTTCTTTTTTTG2220.10388006064349486No Hit
GCTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2220.10388006064349486No Hit
CTTAGGGGTGGCTGTGATTGGAGTTCCGGATATGACATGGGGCCAGCGGGTC2210.10341213244239804No Hit
ACAACAGGGGCATAATGTTTTTAGGGTTTATTCATGTAGCATGTACCTGTAC2210.10341213244239804No Hit
CTTTAAGGGGTGAGGCTCCCGGGGTACTACGACAGCCCTCGAGGTGAAGGAT2210.10341213244239804No Hit
ATACTAGGGACTGTTCCAAAGAACATATTTGTTACTGTTTTCATAGGGAATT2210.10341213244239804No Hit
CTCATAGGGTCTACGAGTGGGATTGTAAACAGCAGAGAACTCTAAACCACCT2200.10294420424130121No Hit
ATTGCGGGATTATGGAGTAGTTGATTCAATGAAAAGAAAGCCCTAATATCTA2200.10294420424130121No Hit
GCTCCAGGGGTAATTGAATGATTATTTAATATATAGTGCTATCAAGCAATCC2190.10247627604020439No Hit
TTTGTAGGGCATCCAATAGTTATTTTTAATATTTGTATTCTTGTTATTTCTG2190.10247627604020439No Hit
TCCATTGGGACAATTTACAGTGTACTTTGTTAAAAACACTGCTGAGGCTTCA2190.10247627604020439No Hit
TTGCCAGGGGTACTGTATTCTCTGGTTTGCCTTTCCTCACTAGAATGTAAGC2190.10247627604020439No Hit
GTCACGGGGCCAGAGCAGTCATCGTGATCAAAGTGCCGTCAATTATTAGGGC2180.10200834783910756No Hit
TGTCAGGGGGTCTTACTGGATTCTGCCAAACGTTAAACCCTTCAGTCCTTCT2180.10200834783910756No Hit
CGTTCAGGGAGGTCATTGTGAACTTAGAGTTTCAGTGGAGTGTTATGATCTG2170.10154041963801073No Hit
TAACAAGGGCACTAGTTAGCATTTCATTTAAGTGGAATCATACAGCATGTAG2170.10154041963801073No Hit
TTGTAGGGGAATGCCCCAACTAAATACTACCGTATGGCCCACCATAATTACC2170.10154041963801073No Hit
GCCACTGGGCCCAGCCAGAAGTGGGAACTTTCAGATGCTGCTGGAGTGAGTG2170.10154041963801073No Hit
TGCGCAGGGGTAGAAATGAAAAATGAAAATTAAAGCTAAAGCCTTTTTATCT2160.10107249143691392No Hit
ATAGCAGGGATTGCAGATGTTTTTATAAATACTTTCATTAAAATGATGTAAA2160.10107249143691392No Hit
AAACGGGGGGTTGAGGAAACGGGTATTTCATGTCTCAGGGAGTAGGTTTGTG2160.10107249143691392No Hit
ACTAACGGGACTCTATTTTGTGTAATGAAATTTTAGCTCTTGTCTATTGAAC2160.10107249143691392No Hit
GTAATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2160.10107249143691392No Hit
TACAGAGGGACATGAAGATATGCTTGCATGTTATATTATATACACATGATTT2160.10107249143691392No Hit
GGCATTGGGAAATCAGATAATGAAAGTAAAAATGAAACAGTGTCTTCAAACT2150.1006045632358171No Hit
CTGCGGGAGCCACTGTGTGTCATATTTGTTCTGAGAATAAAAAATAAATGGC2150.1006045632358171No Hit
CGCAATGGGCAGTAAATCAGCCAGCGAATACCCGGAAGCAAGATGCACAGGC2150.1006045632358171No Hit
AGAGGGGCAATGCAGCAAAATATAAGTTATAACATTTATACACCTAAAAAAC2140.10013663503472027No Hit
CGTGCTGGGATTACAGGCGTGAGCCACCACACCTGGCCCACTCTCACTCTTT2140.10013663503472027No Hit
CTTCCAGGGGCTTAATCTGGGGTATCAAGCCAATTTCCCTGTGATCTGGGTT2140.10013663503472027No Hit
TAAGATGGGAGGTGATACACCCTTAACCAGAATGCTGCACTTTCTCTTTAAG2140.10013663503472027No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)