FastQCFastQC Report
Sat 31 Dec 2022
SRR5845479.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845479.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213204
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGC7640.3583422449860228No Hit
CTTATACACATCTCCGAGCCCACGAGACTCAGTGTGATCTCGTATGCCGTCT6860.32175756552409895No Hit
ATTGTGGGATGACAAAGACCTCAAAAGCAAATGCAACAGAATAAAAAAATAG3260.1529051987767584No Hit
GTTTGCGGGTAAAACTTTTTTGTATTATTGTTTGTTTTGTGTTCAGTTCAAA3260.1529051987767584No Hit
ACTATTGGGACACACTGACATTGTAATTTAAGTTGATGTATTTTTGATTCTG3230.15149809572053058No Hit
GCTATAGGGAGAAAGGGAACCAGGAGAAGTTGTGGACAGCCTGGTAGGCAAA3170.1486838896080749No Hit
CTTTGTGGGAATACCATCAGTTACAAACTTTGGGACATTACTTAACCTCTCT3080.1444625804393914No Hit
TATCTAGGGCAAGAACTGGAAGGAACTCCCTGCTGTTTTAGAAAAAGAGAAA3070.14399354608731543No Hit
TTGAGCGGGCATGTGGATCTAAATTTTAAAAATAGGAATGAATCTCTTAATT3020.14164837432693572No Hit
CTAGCGGGGAGATTCAGTCAGTCTTCTATGGTTAATAAAATCTATAGTGAAC3020.14164837432693572No Hit
GAACCCGGGTGGCAGCTGTGGCGCCGACATGGCTGCGCTGGTGGAGCCGCTG3000.1407103056227838No Hit
TGTGGGCGGGATTCACATAACATAAAAGTAACCATTTTAAAGTGTACAATTC2990.14024127127070787No Hit
TTTATGGGGAAAAATGCATTTTCCAAACAACTTATACATGCTTTTTATGACT2960.13883416821448002No Hit
GTAGTCGGGTGCAAAAAAATAAATAATGGGAAGAAAGGGCTGTATAATGAAA2960.13883416821448002No Hit
AACTCCGGGGAACTCCTTCATAAGCAAAATTATTTTTATGTGATCTCTAAGA2910.13648899645410031No Hit
AAGCCTGGGAAAGAACTGAAATCATAACCAACAGTCTCTTGGACCAGAGCAC2900.13601996210202436No Hit
TTTCTTGGGTGGAGATCCCAAATTTATAAAACAATTACTACTAGACATAAGA2900.13601996210202436No Hit
ACTTATGGGATCCAAAATTAATAAGTATCTGTTGGCCTCTGTTTTCTCCCAG2900.13601996210202436No Hit
AAAGACGGGATTCCTGGTGTGTTTTGGAGGGATAAATCTTGGGAGACCCTTT2890.1355509277499484No Hit
ACACTGGGGGACTCAGACTTCTGAGGAATTTGGGTTTGTTCCCCTTTGTAGA2890.1355509277499484No Hit
GGTACAGGGCTCTACAGGAACTTAGTTCTTGTATGGCAACTTGAGTGGCATT2870.1346128590457965No Hit
GCTGCTGGGAATTTAGGATGACATACAAGAACAATTCACTTACTAATAATTT2730.12804637811673328No Hit
GAAGGCGGGTCATACAGCTAACTCACCTATCTCATCCATCAAACTTAGTTTC2720.12757734376465732No Hit
ACAAGCGGGTCCTTTAGATTGCTGGAGCATATTCTGTTGCATTTTGTTTATC2700.12663927506050543No Hit
GGGCCAGGGAACTGGACACACTTGGAAAATGCCTACATCATAAACCTAAAGA2700.12663927506050543No Hit
TTTACGGGGCACAAATAGAATAAATACATGAAAACTTGGATTAAGTCAGGCT2690.12617024070842947No Hit
GAATCCGGGATGTGAATCAACAATGAATAGTCTCATTTTGGACATGGAAAAA2690.12617024070842947No Hit
GCATGTGGGAGATTACACTGGATGAATTCAAAGAAGCTGCAAAGAGCGACCC2660.12476313765220164No Hit
GGCTCGGGATCCCTTCTTGCCATTCTGTCATCTAAGCGCCCATCTGGCTGTA2650.12429410330012572No Hit
TACACCGGGGAGTGGCCGAGCCAAGGAGCTTCTGGGCCAGGGCCTGCTGCTG2650.12429410330012572No Hit
CCAAATGGGGGAAGGGGCAAGACGGGTTCAGTTCGTCATGGGGCTGTTTGGA2640.12382506894804976No Hit
CATATCGGGGAATCAGTATTCCATAGTATTTGCTTTCCTTGTATCTTCTTGA2630.12335603459597382No Hit
TACTAAGGGGGAAGTAAAAAAAGGTCTAGATAACCTCTACAAGAAAGTTGAT2630.12335603459597382No Hit
TAGTAAGGGCAGATCATTGCTACTCAAAATGTGATCCATGGGCCAGCAGCAT2630.12335603459597382No Hit
TCTACCGGGTGAAAAAAAGGATGCAAAATAAAAAGCTGCCTAAGGTGAAAGT2620.12288700024389787No Hit
GCCCAAGGGGTAACATTTCAGATAAATGGACACTTGCCATTTTCAGTTTTAA2600.12194893153974597No Hit
ATTATTGGGGAAGTAGGTGAACAAAGCCAGAGGACTTACATTTCCAGATACG2600.12194893153974597No Hit
CAATCAGGGCAACTGAAAACTTAGGTTGTTTCTAAATTAACTATTGTCAAAC2600.12194893153974597No Hit
ACTTTAGGGAACCACGGAGATAATCAAATTTCTTTGTCAATCATGTTTCTGA2590.12147989718767002No Hit
GGCGCAGGGAGTGTCTCTTACTGTACATTTTCTCCCTAATTCCTATGGCATT2590.12147989718767002No Hit
GTGAACGGAGGGATGAAATTGCACTATAGCCACAGCGTTCTGTTTCTTAAAA2570.12054182848351813No Hit
GACATCGGGGAAGAAGAAGGGATGATGTGTGGTAGAGAAGATGAGGGTTAAC2570.12054182848351813No Hit
CGGTATGGGCTTACTCGGGAGCTGGCTGAAGATGGCTACTCTGGAGTTGAGG2570.12054182848351813No Hit
TGCAGTGGGGTCACCAAAACTAGGACATTTTTGAGAGTAAAAAGAGGCAATC2560.12007279413144217No Hit
AGCGCTGGGCAGATTTTCCTGGTACCCCACAAATAAAGCAAAAATATAGAAG2540.1191347254272903No Hit
GTGCAAGGGCGGCCCGACTCCTCCTGTGTTGAGACTAGTCATCTACTGTTCC2530.11866569107521435No Hit
CCAACCGGGAGTGGCTTCCAGACTAAATAAAAACAGAACATTTTATCCAAAT2520.11819665672313841No Hit
CGTAACGGGATTCCCTGGGATGTTCTATTAATAATAAAGTGATAATAAATGA2520.11819665672313841No Hit
ACAGTCGGGTAAATCGAAAAATGTTGATGGCTTTCTTGTGTCTTTATCAAAT2520.11819665672313841No Hit
GTACAAGGGTGGCATTGGAATTTTCCAGTACAATTTTCCATTATCTGCTCAA2510.11772762237106246No Hit
ATTTACGGGTGCCTCTCGGGCTCTGACAGTGTCATTGACACTGTTGCTGATC2510.11772762237106246No Hit
AATGTTGGGATCTCCCTGGTGAGAATGCAGATGCCCTGGGCTCACAGGGCAA2510.11772762237106246No Hit
GGCATTGGGAAATCAGATAATGAAAGTAAAAATGAAACAGTGTCTTCAAACT2500.1172585880189865No Hit
GAATTTGGGCACACAAACTCCAAGGTGTTTTTAAGAAGACCAGTGAGGGATC2500.1172585880189865No Hit
TTCTAAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA2500.1172585880189865No Hit
TAAACTGGGATAGTTGGCATCCAGTAATCAACAATAAATAGGGAAAAAATCA2490.11678955366691056No Hit
GTGCATGGGTCTCTTTTAATTTCAGCTATCATTTTTAGAGCCTTACAGTCAC2490.11678955366691056No Hit
GCCACTGGGCCCAGCCAGAAGTGGGAACTTTCAGATGCTGCTGGAGTGAGTG2480.11632051931483461No Hit
TCCATTGGGACAATTTACAGTGTACTTTGTTAAAAACACTGCTGAGGCTTCA2480.11632051931483461No Hit
TGAAATGGGGGGGATAAAAAGATTAAGAGATTTAGCTAGTGATACACAAGGA2470.11585148496275867No Hit
GCTATCGGGCACACACACAGAAGAAACCCTCAGAATACGCTTAACCAAGGAT2470.11585148496275867No Hit
CTTATAGGGGAGCTGACCCAAGGGGCCTTCGAGGTGCCTTAGGCCGCTTGCC2470.11585148496275867No Hit
TGGCACCGGGAGGCAAACCTGAGGTCCTCAGAATGGCGGGCACAGGTTTGGT2470.11585148496275867No Hit
GATTATGGGTTACCCCAAAATACTTGGCTTTGAATGGTTCATCTTGAGTGTG2460.11538245061068274No Hit
CCAATAGGGAAGACATTTCAGTTAAGCTCATAAAATTTCATTGTTTTCATTT2460.11538245061068274No Hit
ATACTAGGGCATTTTCCCACTTTTCTATTGAAATATTTTTATTTTTCTAAAT2460.11538245061068274No Hit
CTGGTAGGGCTTGCCTTTAAATGCTAGCTCTTATTGAGAGACCTCAGGCCAG2450.1149134162586068No Hit
AAACAGGGCAGCCTCCATTTCCATTCCAGCCACAGAGCTTAAGAAAAGGGCT2450.1149134162586068No Hit
TAATATGGGATAATAAAAAAGCATTTCTAGCTACAAAATAACACAAGGAACA2440.11444438190653083No Hit
CACTTTGGGCTACATAAGTAATCTGCATATTTTTAATGCCTTCATTCATGAA2440.11444438190653083No Hit
ATTGCCGGGAGTCCAGTTATGAGTAATGAGATTCAATCAGTAATTAAAAGTC2440.11444438190653083No Hit
GAATTGGGGCAAATTAATTGTGTGAACTTGGAAGCACACCAATCTTACTTTG2440.11444438190653083No Hit
AGCTTAGGGAATCGATATTATTTCTATTATTTTCATTAATTTCATTAAGGGT2440.11444438190653083No Hit
TGTCAGGGGGTCTTACTGGATTCTGCCAAACGTTAAACCCTTCAGTCCTTCT2420.11350631320237894No Hit
TTCTTTGGGATAGTAGATTTAAATTATTTTAGACTTCTTGTTTGCAGTTCAT2420.11350631320237894No Hit
AATGATGGGATTCTAATACAGTAAGATATTGGACACAAAATGGAGGTAACTT2420.11350631320237894No Hit
GCCCAAAGGGAAGCTGATTGCAGGGAAGATCATCCCAGCCATTGCCACGACC2420.11350631320237894No Hit
CTTGCAGGGGTAAATGAACTTTAATCATGGCTGTTAATTGTAAAACATGACT2410.113037278850303No Hit
TTGGCGGGGCTCAAGTTTATGTGTGTAAACTGAGGCAGAAATGTTAATTATA2400.11256824449822704No Hit
TACAAGGGGATCACCATCCTCTGCATCGATCTGGGCACTGACATGGTCCCTG2390.1120992101461511No Hit
GTCCCCGTGCCCGAGGACTGGGAGGATGGGGCTGAGCCGCTTGCCTGACTTT2390.1120992101461511No Hit
CCACTTGGGACTTGAAGCAAATTAAGATCTTTTAAGCCTGGACTAGCAAAGG2390.1120992101461511No Hit
TGCGCAGGGGTAGAAATGAAAAATGAAAATTAAAGCTAAAGCCTTTTTATCT2380.11163017579407515No Hit
GCTACAGGGAGCTTGCACTTTGCTTTAATTTCTCTGAGCTGCAAGAATGTCT2380.11163017579407515No Hit
GCTGCATGTTTCCAGAATTTTAAGCTATTATCACTTAAAGGTTATTACACTC2380.11163017579407515No Hit
AGCCAGGGCGGGTCGTGTCGTGTCCTTTTCTCTCTGGCTAGGCAGGTGTGCC2380.11163017579407515No Hit
ACACCAAGTTCCTCCCAGATTAAACTTCCCAGGTGTTCAGAATTACTTTTGC2370.11116114144199922No Hit
TCCATTGGGAGATTTGAAAAGCTGACTTTTTAACATCTTGAGGCAACTGTAG2360.11069210708992328No Hit
GATATCAGGGAACTTCTTCTGGATATATTTTGTGCAACAGCATTTTAAAATT2360.11069210708992328No Hit
GACCCAGGCCGTGCACGAGCTCCTCGAGAAGGAGAACATCACCGACCCGCCG2340.10975403838577137No Hit
ACTAACGGGACTCTATTTTGTGTAATGAAATTTTAGCTCTTGTCTATTGAAC2340.10975403838577137No Hit
TAGCAGGGTTAGCTATAGTAATATCAGAGAAAATAGACTTTAAGTTAGAGAA2330.10928500403369543No Hit
ATTGCGGGATTATGGAGTAGTTGATTCAATGAAAAGAAAGCCCTAATATCTA2330.10928500403369543No Hit
ACGCAAGGGACTGTCCGAGCACGAGAACGTGCTGGCTGAGTTCAGCAGCGGG2330.10928500403369543No Hit
GCTGATGGGAGCCTTCCAAAGCTCAGGGATTACAGACATGAGCCACAGCACC2320.10881596968161948No Hit
GTCTTTGGGGGTCCCTGTGACCGGCTTGGCGGTTGGAGCGGCTGTCGCCATG2320.10881596968161948No Hit
CTTAGGGGTGGCTGTGATTGGAGTTCCGGATATGACATGGGGCCAGCGGGTC2320.10881596968161948No Hit
TGATGCGGGGATGCAAAATAAAACAACCCTCAGTTCATACCTAGCTCCCTGT2320.10881596968161948No Hit
TTTACTGGGATATGTGTTGATTCTTGAAAACTGGGACGTGTATTTCCATTGG2310.10834693532954354No Hit
TTGTAAGGGCGATCTATGCGTGGGACAATTAAAATACTCATAGTAAATCATT2300.10787790097746758No Hit
CCTTGGGGCTCTTTCCCTAAGCAGCCTGAGGGTTGACTGGATTGGTGAGGCC2300.10787790097746758No Hit
CAGATGGGAGAAAATGAAATTAATATGAAGTCCAAATAACGATTGCATAAAT2300.10787790097746758No Hit
CTCCATGGGGAATTTCTCCATTCATATTTATAACCATTCTAGTTTTATCTTC2300.10787790097746758No Hit
GTTGCCGGGATTAAAGTAATAATGTGTTCCTTGAGGATAACTTGTCAAATGC2300.10787790097746758No Hit
ACGAATGGGCAGAGTTTTCAACTCAATATGACACAGATAAATTCACTTCAGT2300.10787790097746758No Hit
TAAGCAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2290.10740886662539163No Hit
AACCCAGGGCTTGATCCCAACAAAATGATTTACATTATAAATTCACATTATG2280.10693983227331569No Hit
ATATGGGGGGTATGTTAGCAATAAGTTTTAAGTGTTTGTTTTTCTTTTTTTG2280.10693983227331569No Hit
ATAGCAGGGATTGCAGATGTTTTTATAAATACTTTCATTAAAATGATGTAAA2260.10600176356916381No Hit
ACGTAGGGGACTTGCTCTTTTAGAGATGATTGAATTTTATTATTTTTCCCCA2260.10600176356916381No Hit
CTTTAAGGGGTGAGGCTCCCGGGGTACTACGACAGCCCTCGAGGTGAAGGAT2260.10600176356916381No Hit
TTCCAAGGGATTTGATAGCACAACACAGTGACTACAGTCAACAATTTATTGT2260.10600176356916381No Hit
TTTAGAGGGCTGGCAAGAAATATGACTTGCTTTTTGCTGAGCTTAATTCAGA2250.10553272921708787No Hit
CGTGCGGGGGGTCAAGGCCGCGCTCGCGGCGAGGGGCTGCGAGAGTGACCGC2250.10553272921708787No Hit
AGAGGGGCAATGCAGCAAAATATAAGTTATAACATTTATACACCTAAAAAAC2250.10553272921708787No Hit
TTACCTGGGACGAGGTGTGGCTGCAGGAGCAGGGGGAGAACACCTACGTGCC2250.10553272921708787No Hit
CAGCCACGGGAGAGTTTTGAATGTACTAGTCCTTAGTTTCCATAGAACTGAA2250.10553272921708787No Hit
TTAGTTGGGAGACTTTGTGGACCATCATCCAATTGTTTTTTGGAACCTGGTA2250.10553272921708787No Hit
AACAACGGGCAGGAACTGTGTTCTTGCCACGACTCCTCTTAGGAAAACGCCT2250.10553272921708787No Hit
CTTGGCGGGGAGGTACATTTCTTTAAGCACAAGAGAAGTAGAAATGGCCTTT2240.10506369486501191No Hit
CTGCAAGGGATACACATTTTCTAGCAGAATTTTTAAAATTTGTAAGTAGCAT2230.10459466051293596No Hit
CGTTCAGGGAGGTCATTGTGAACTTAGAGTTTCAGTGGAGTGTTATGATCTG2230.10459466051293596No Hit
TTAGTTTGGGAATGTTCTCAAAGGAAACAGACTTGAGTTGTTGGATTAGAGG2230.10459466051293596No Hit
TTAAAAGGGAAAGTAAAAATTTTAGATGCCTTTTTATTACTTGTTACTATAG2230.10459466051293596No Hit
ACAAATGGGACAACAGAAATGGATTCTCCTTTGATATAAATGTCAGTCCTTG2230.10459466051293596No Hit
AATTAAGGGCAACATGGGAAGTGATGAACTGAGGATTTGAACCCATGTTTGT2230.10459466051293596No Hit
GCATCTGGGGACCAATATAAGTATAGCTACTTGATATGGTTTGGCTGTGTCC2230.10459466051293596No Hit
TAGAACGGGGTAAACTTGAAAGCAAGACCTTGATTGCACCAACAGGTCCAGA2220.10412562616086002No Hit
GCGTAAGGGTCCTCATTCATTCATCAGTGTCCAGTTACTTCTGACTTCTGGG2220.10412562616086002No Hit
AGTTACGGGGTTTAACGGCCGCGGTACCCTAACCGTGCAAAGGTAGCATAAT2220.10412562616086002No Hit
TAAGTGGGGCGGTAGGAAACACAGTTATTCACAGTGGCCATCCCCAGCAGAA2220.10412562616086002No Hit
GTAATAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2210.10365659180878407No Hit
CTATATGGGGAGTCAGTTCCGCGGTAGAGGTGACCTGACTCTCTGAGGCTCA2210.10365659180878407No Hit
TGTTACGGGGAACAGAGTCTAGAATTATGATAATCATATGGTTTCCGCGAGA2200.10318755745670813No Hit
GTTGCTGGGGGACCTGGCCGAGCAGGAGGCGCCATCATGGGAGTGGACATCC2200.10318755745670813No Hit
ACATATGGGCTGAGGAGCTGGCTCTTAATCCTGGTCTTTAATTTCTGACATT2200.10318755745670813No Hit
GGTGCTGGGAAGGGAGGAAGTACAGAGGTAATTTATAGAATTCCAGAGTAAT2200.10318755745670813No Hit
ATCACCGGGGAGGACTCTTTGTCTTTACGGAGTTCATTATTCCAAGTGCATG2190.10271852310463217No Hit
ATTGCAGGGCCTGAGTAGGCCTAGAAATAAACATGCTAGCTTTTATTCCAGT2190.10271852310463217No Hit
TGATATGGGGTAACTTGATTCCACAGGGTCAAATAAAGAAAACAAGAAATGG2190.10271852310463217No Hit
AGTTGAGGGACATCATAGCCAGCAACAGCAAGAAACATAGCATAAGGTTTGT2190.10271852310463217No Hit
TTTAGCGGGTTGAATAATCTTTCCTGTTCTGGTTCTTTGAGAAACCCTAACA2190.10271852310463217No Hit
TTCTCTGGGTCTCAAGAGGTGAAATACAGAAAGCCTTTTTTTCTTGATCTTT2180.10224948875255625No Hit
GATCCTGGGAGCTGATGTCAATGCTATGGACTGCACAAAAGTCTCAATGTTC2180.10224948875255625No Hit
ACACGACGGGCAATGGATATAAAACATCTACCATTTCCAGCTTCCAAACTGT2180.10224948875255625No Hit
CCTGTGGATTACCACATGCATTAAAAAATTTTTCAAGCTGGGCCTAGTGGCG2180.10224948875255625No Hit
AACATCTGGGGATCATAACTAGTAATATGAGTTAATGTTGAATCTTTTTTGA2180.10224948875255625No Hit
CTTATTGGGCATTTCTTGAAGGACATGGAGTGGAGATTGGATCATTTAACAA2170.1017804544004803No Hit
CGTAAAGGGGAACTTGGCTCTGGATAAAATGGACCTAACCAACAGCTACAGA2170.1017804544004803No Hit
AGACTTGGGGTCATTAATATCCACAGAGACTGTCTTGAGAATGGAAAGGAAG2160.10131142004840435No Hit
GCCTAAGGGACAGGAAGACAGTGCCCCGGTTTGTTATCCAGGCATAAGACCA2160.10131142004840435No Hit
GAGGCACCCCCTATTCCTGCTCCCCCAACTGGATCAGGTAGAACAACAAAAG2160.10131142004840435No Hit
GTGGTTGGGGTGTAGTCATAAATAGACAACCATCATTATTTAAATGGCATAA2160.10131142004840435No Hit
GCTTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC2160.10131142004840435No Hit
GTAGGAGGACCACAATCAATGTCTTAGACACAGAGGCAAAGGATGCTTTGGA2150.1008423856963284No Hit
GAGGAGGAGGCACAGACGCAGCTTGAGGAGGACGTGGCAGCGGTGGTGCCAT2150.1008423856963284No Hit
TTTGTAGGGCATCCAATAGTTATTTTTAATATTTGTATTCTTGTTATTTCTG2150.1008423856963284No Hit
TCTTTGGGGGGCGGGAACCGGGCGGCGGCCAGAAAAGCCGCGCCTGTAGTCC2140.10037335134425246No Hit
GTCACGGGGCCAGAGCAGTCATCGTGATCAAAGTGCCGTCAATTATTAGGGC2140.10037335134425246No Hit
TGAAATGGGTCATGCCTGTAATCCCAGCACTTTGGGAGGCCAAGGCGGGCGG2140.10037335134425246No Hit
ATATGTGGGCTTGCAGAAATAATGAGGCCAGTAATGATAAGAGTGCCTAGTA2140.10037335134425246No Hit
AGCTTTGGGGACCTCCTGCTGATGCTTCTCATATCATTTTCTCCCCATGGCA2140.10037335134425246No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)