FastQCFastQC Report
Thu 19 Jan 2023
SRR5845459.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845459.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106712
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT4230.3963940325361721Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC3550.3326711147762201No Hit
TCACTGGGAGGCGCGGTGAAACCATCGAGACGGAGGGCCAGAGAGTCACGGC1780.16680410825399206No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC1710.16024439613164407No Hit
CTTCCAGGGATGCAATAGAGATTTCATAGTTTGCTTTCTTTGCTCTTTAAAT1360.12744583551990404No Hit
CGTTGAGGGGGAAGTTATGACTTTAAAGAATAAAAACCTAAGCAAATGCTTG1360.12744583551990404No Hit
TATCTTGGGCAAAGGTGGCTGAGAGTGAGGAAAGTACACAGAATTTTAAGCC1240.11620061473873602No Hit
AGTATGGGATTAAATCAGAGTCTTTAATTCTTGCATGTTTGTATCTAATTTC1240.11620061473873602No Hit
GTAGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1230.11526351300697203No Hit
GGCGATGGGGAAGGTGTAAGGAGGGGCTTGCTAAGAATTTGAGCACATGTAG1220.11432641127520804No Hit
AACGCCGGGAATTTACTTCTTTGGCAGGTTTAGAAAATTAGATGCATACAAT1220.11432641127520804No Hit
CCATAAGGGGATCTGTATCCGGGCTCTGAAATGAAATACGTCTCTTCACCAT1220.11432641127520804No Hit
GAATCTGGGAATCTTTGCCTCCCCTGACAAAGACAGCTATGACCCTGACTTC1210.11338930954344403No Hit
GACATAGGGAAAGTATATGAATCCAATTAAAGTTGTTCTGTGAGTGTGTTTT1210.11338930954344403No Hit
AGTTGCGGGTTAGTCTATAGGGATGTTCCATGTGTGTATTTTTATCACTCCT1210.11338930954344403No Hit
TTTCCAGGGCAGCTAGATTCAAATGGGCTGATAACCAAATTTTAACACATCA1210.11338930954344403No Hit
GCACTTGGGTCAACCATAAGCTTAATGGGAAGATCAAGAAAAGAGAGAGTCA1200.11245220781168003No Hit
AGTGCTGGGGCTCTTTTTCCGGCTGGAACCATGGAGGGTGTAGAAGAGAAGA1180.11057800434815203No Hit
ATCTACGGGAGAGTCAACTACCGAAGGAAGACAATTTTCGTTTGGTCTAAAA1170.10964090261638804No Hit
TTGTAGGGATTCCAGAAGTTAGTACCTGGTGGCAAAGCTACTTTGGTGATGA1170.10964090261638804No Hit
CTACTCGGGGCAGACAGAGGAGAGATCCCTGGGACCTGGGCAGTCTGAGCCT1160.10870380088462404No Hit
GCTCGCGGGTACTCCATGCAGGGACTCTGCAAGTTTGGCTTTTATGAAGTCT1160.10870380088462404No Hit
TGACAGCGGGTGCTCAGATGCTACTGTGAGCTTCCTGTCCGGTGTTAGAAAG1150.10776669915286002No Hit
GGTTAGGGATTTCAAAATGCCACCTCTTCTGATTCCAGATACAATTATCTTT1150.10776669915286002No Hit
GATGTAGGGGATCGAGGAGCTCTGCGGCCGCCAAGCTGCGCGGGCAGAAGCG1140.10682959742109603No Hit
TTATAAGGGACTTTATTCAAAGAGCAGCCCGCTTTGTGTGACTAAAATGAAA1140.10682959742109603No Hit
TAAGTTGGGGACCAGAACTGTGTCATATTCCTAAGAGGAGTGTGTGTTTTCA1140.10682959742109603No Hit
GCCACTGGGAACCCCAAGCTGAATAAATTCAGGGAGCTGGGGAGAAACCAGC1140.10682959742109603No Hit
TTTGCAGGGTAAACAATCTATAGCACCATAAATTAGCATTTTCTAGTGTGAC1130.10589249568933204No Hit
TAACGCGGGATGTGAATCCAGAGATAAGTTTATACGGGAATTATTTGAATCA1130.10589249568933204No Hit
TGCAGTGGGTTGGCATTTACAAGAGATGTTGGGTGTCAAAGTAAACATTGTA1120.10495539395756805No Hit
ATCGCAGGGCGAGCCGAGCTGGGCCAGCCAGGCAACCTTCTAGGTAACGACC1120.10495539395756805No Hit
CGCTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1120.10495539395756805No Hit
GCTACGGGTGTCTTCTTTTGAAAAGTGTCTGTTATGTCCTTTCCTCACTTTT1110.10401829222580403No Hit
TTAAGGTGGGAACAAAACAAAATCAGAACACAAATAATGTCTTAGAATGCAA1110.10401829222580403No Hit
TGTAGCGGGATCAAGTTTGCTTAATAAAAGCTATGAGACAAATAGTCCTCTA1110.10401829222580403No Hit
CACACGGGGGCCAAAAATAGGACTAAGTCTTAAACTGGGTGCTTCCAATAGT1100.10308119049404003No Hit
AAAACAGGGCCACCTGTTAACATTTCCATATAAGTGAATCCTGCTTTTGGTG1100.10308119049404003No Hit
ACCATAGGGATAAGATATTAGAGGTAAAATACAGTTTACTCATTCTTTTAAC1100.10308119049404003No Hit
CGTGGGGGGTGATGCAGTGATAAAGCGGAAACATTGCTCTGCTACCTGCCCA1100.10308119049404003No Hit
GTCCGGGGGATACTGCACGTGATTAGAATGTTTTTGAAGGTTTCCTCGTTTT1100.10308119049404003No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTA1100.10308119049404003No Hit
TTTCTGGGGGTCTTACTTTACCAATAACGTGTTTTTCATTTTAATCTTTAGT1100.10308119049404003No Hit
TCTAAGGGGGGATTTTGATAGAACTGCATTGAATTTGTAGACCAATTTTGAT1090.10214408876227604No Hit
ACACAGGGGACATGTATCACTGGAGTTGCTTCAAACACAAAGAACATTGCCC1090.10214408876227604No Hit
ATCATAGGGTGAGAGACATGCATGTGAGTAATTGTCAACCATATAAAATCCT1080.10120698703051202No Hit
CTAATGGGGGAAGACAGCCCCACACGGACCAGTGTGCAGAAGGCTCCGGGAA1080.10120698703051202No Hit
ATAGCTGGGCAAGATCTGCAAGTATAGCTAAACCATCATTTAAAAAACAGTC1080.10120698703051202No Hit
CACGCAAGGGTCTGTGCGTGGGTGGTGATTCTGAAGCTTATATTGGAGCAGA1080.10120698703051202No Hit
ACATGGGGACTCTTTTAGAGGACGAATCCCTGCAGCTGGGAGAGAGGAAGCG1070.10026988529874803No Hit
TTTCCAGGGAAGAGAGGTAAGATCATCACAAATCCTAAATATATTAAAAGCA1070.10026988529874803No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTCG359.904397E-846.1023981
CTACTCG405.4205884E-946.1023941
TGCCAGG206.2170567E-446.1023941
TTTCGCG206.2170567E-446.1023941
CGTTGCG206.2170567E-446.1023941
GCTATAG206.2170567E-446.1023941
TAACGCG206.2170567E-446.1023941
GCTGACG206.2170567E-446.1023941
TCTACCG206.2170567E-446.1023941
TACGAAG206.2170567E-446.1023941
AACGCCG206.2170567E-446.1023941
CCATAAG301.8173796E-646.1023941
CATAATG206.2170567E-446.1023941
GTAAGCG206.2170567E-446.1023941
GATTCCG206.2170567E-446.1023941
GGTTCCG206.2170567E-446.1023941
GCATCTC206.2170567E-446.1023941
AAATGCG253.3518438E-546.1023941
TTTCGGG301.8291848E-646.0591283
ACCTCGG359.9780664E-846.0591282
Produced by FastQC (version 0.11.5)