FastQCFastQC Report
Sat 31 Dec 2022
SRR5845458.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845458.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences106594
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT3760.3527403043323264Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC3720.3489877479032591No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC1770.1660506219862281No Hit
AAAACAGGGCCACCTGTTAACATTTCCATATAAGTGAATCCTGCTTTTGGTG1580.14822597894815845No Hit
CTTCCAGGGATGCAATAGAGATTTCATAGTTTGCTTTCTTTGCTCTTTAAAT1470.13790644876822336No Hit
GCTCGCGGGTACTCCATGCAGGGACTCTGCAAGTTTGGCTTTTATGAAGTCT1400.13133947501735557No Hit
AGTATGGGATTAAATCAGAGTCTTTAATTCTTGCATGTTTGTATCTAATTTC1390.13040133591008873No Hit
TTATAAGGGACTTTATTCAAAGAGCAGCCCGCTTTGTGTGACTAAAATGAAA1380.12946319680282192No Hit
TCACTGGGAGGCGCGGTGAAACCATCGAGACGGAGGGCCAGAGAGTCACGGC1340.12571064037375462No Hit
CCATAAGGGGATCTGTATCCGGGCTCTGAAATGAAATACGTCTCTTCACCAT1320.12383436215922096No Hit
TTTCTGGGGGTCTTACTTTACCAATAACGTGTTTTTCATTTTAATCTTTAGT1320.12383436215922096No Hit
GCTAATGGGACAACTGAAACTTAGCATGTGAGTTAGAGGAATGTAATATTAA1270.11914366662288685No Hit
TCATCCGGGGTTGAAACTATAATGGGATAAGGAAACATGTAATCATGGAGAA1260.11820552751562001No Hit
GAGTCGCGGGATGATGAATGGGCACGGTTTGGCCGAACACTTGCAGAAATTA1250.11726738840835318No Hit
CTACATGGGGAGTGTGCCATGGACATTGGGATTTTAAAAGTTCCCAGCCCAG1230.11539111019381953No Hit
TCTCCTGGGATGAGGACTTGCTTTCTTTACCTCCGGTTCTTTCCATGTCTTA1230.11539111019381953No Hit
ATAGCTGGGCAAGATCTGCAAGTATAGCTAAACCATCATTTAAAAAACAGTC1220.11445297108655271No Hit
TGCAGTGGGTTGGCATTTACAAGAGATGTTGGGTGTCAAAGTAAACATTGTA1200.11257669287201906No Hit
ACACAGAACGGTAAATTCCCTAATTAGTGCAGTCAGAACTGAAGAGTTCTTC1200.11257669287201906No Hit
GCTACGGGTGTCTTCTTTTGAAAAGTGTCTGTTATGTCCTTTCCTCACTTTT1190.11163855376475224No Hit
TTTCCAGGGAAGAGAGGTAAGATCATCACAAATCCTAAATATATTAAAAGCA1190.11163855376475224No Hit
ACACAGGGGACATGTATCACTGGAGTTGCTTCAAACACAAAGAACATTGCCC1190.11163855376475224No Hit
GGTGCTGGGGAAATGCTGTCAGGTTCAAGTAAGACAAAATATTTTAACAGCT1180.11070041465748542No Hit
GTCGCAGGGAGAAGCTGAGCTGCAGAGATTACTCCTCTCATTAAATAAAGGA1180.11070041465748542No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTA1170.10976227555021859No Hit
GTATCAGGGTAATTTATATATCCCCTTGCTGAATTAATTCCTTTATCATTAT1170.10976227555021859No Hit
ATGTCCGGGTGGCTTGTAGAGTGGTACTTAAGAAAAATGGGATTCTACCTCT1160.10882413644295176No Hit
GATCTGGGATGCTACAGGGGCACATTTTCTGTGGTTAAAAATGATCTCCAGA1160.10882413644295176No Hit
AACGCCGGGAATTTACTTCTTTGGCAGGTTTAGAAAATTAGATGCATACAAT1160.10882413644295176No Hit
CCATGGCAACAAAAAGTCTACTCTTACTTTGCTGTTTATTTTTATCTTCAAT1160.10882413644295176No Hit
CACGCAAGGGTCTGTGCGTGGGTGGTGATTCTGAAGCTTATATTGGAGCAGA1160.10882413644295176No Hit
CGTGCTGGGAATCTAGCTGGAGCTGTTGAATTCAATGATGTGAAGACCTTGC1150.10788599733568492No Hit
TTGACAGGGGCAATCCCATTAAAAAAAGGCTCTTTCCTTTAGAGAAACTCTA1150.10788599733568492No Hit
AAAATAGGGCTGGGGAGCTCACCCATTCATTCCCACCGCTTCAGCCAGCAAA1150.10788599733568492No Hit
TCGCATGGGGCCTTTCGGCCGGAACCGCCATCTTCCAGTAATTCGCCAAAAT1150.10788599733568492No Hit
GGTTAGGGATTTCAAAATGCCACCTCTTCTGATTCCAGATACAATTATCTTT1150.10788599733568492No Hit
TTACGTGGGCAGTTTTCTCATTTATGGAAGAGTACCTATTTCTTTGGGTTGT1140.10694785822841811No Hit
CAGAGAGGGCCTGCTGTGTGTTGGGAAGACATCAGGCCTGAAAGCTGAGGGC1140.10694785822841811No Hit
GCTCTTGAGACACCTGATGACATCTCTGACAAAGCACCACCCCAAATCTCTA1140.10694785822841811No Hit
CAAAAGGGGGCATCCCCCTGCCCTCTCCTAATACTAATCAGGGGATCCTTCA1130.10600971912115129No Hit
TATCTTGGGCAAAGGTGGCTGAGAGTGAGGAAAGTACACAGAATTTTAAGCC1130.10600971912115129No Hit
GGTAACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1130.10600971912115129No Hit
CCAATGGGGCAGGCCAAGGTTGAGGGGAAAAGTAAAGTTAAAGTCGGTTCTC1130.10600971912115129No Hit
CTTGCGGGGGCTGCTGTCCTGGGCATGGTGCAGTACCTGTGCCTAGGATTGG1120.10507158001388445No Hit
TAACGCGGGATGTGAATCCAGAGATAAGTTTATACGGGAATTATTTGAATCA1120.10507158001388445No Hit
TGTTAGGGGTACATGCGGGATGACCCCAATACGCAAAACTAACCCCCTAATA1120.10507158001388445No Hit
GTTGCTGGGCACCTCTGTAAACTATTCCTAAAATGTGTATGATATATTTGAA1120.10507158001388445No Hit
ATACAGGGGTCACAAAACAGGAACCAGCATTCTAATTAGATTTACTATATCA1120.10507158001388445No Hit
TTGAGGGGGGGCTCCGGCTGGTGGTACAGAGGTGCTCGGGGCAGAAGTGGAT1120.10507158001388445No Hit
CGTTGAGGGGGAAGTTATGACTTTAAAGAATAAAAACCTAAGCAAATGCTTG1120.10507158001388445No Hit
CTACTCGGGGATATTAAATTATTCTGTGTTTTGCTTTTCCTTGATATGTAGG1100.10319530179935081No Hit
ATAGCCGGGGAAATCTATCTTTATCCTAGACCTCAAGTTACTTTTAGAAATA1100.10319530179935081No Hit
GACATAGGGAAAGTATATGAATCCAATTAAAGTTGTTCTGTGAGTGTGTTTT1100.10319530179935081No Hit
ACGATAGGGTCTTCTTTTACTCCTTCTACTTACACCATTGTAGGTCATGTGA1100.10319530179935081No Hit
GATCATGGGGTAACTGTGAACAAATAATTTGTTCTGTGCCTTTTGCCTGGTA1100.10319530179935081No Hit
ACGCATGGGGGAAATACCTAGCATAGAGTATTTTAAAAACTTAATTACTCAT1100.10319530179935081No Hit
TTTGCAGGGTAAACAATCTATAGCACCATAAATTAGCATTTTCTAGTGTGAC1090.10225716269208399No Hit
ACCATAGGGATAAGATATTAGAGGTAAAATACAGTTTACTCATTCTTTTAAC1090.10225716269208399No Hit
TTTCCAGGGCAGCTAGATTCAAATGGGCTGATAACCAAATTTTAACACATCA1090.10225716269208399No Hit
GCACTTGGGTCAACCATAAGCTTAATGGGAAGATCAAGAAAAGAGAGAGTCA1090.10225716269208399No Hit
AATTCCGGGTTGCATACCTGATGGCATCTATGTCAATGCTGATTGGGTAATT1080.10131902358481715No Hit
GTAGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1080.10131902358481715No Hit
TAATATGGGGACACCATTTATTAAATGTTATGTTCCTTCTCCAACAGTTTTG1080.10131902358481715No Hit
AGTGCTGGGGTGCCTTTTGTCTATTTATAATGCCACTGGAAGAGGAGGGATA1080.10131902358481715No Hit
CTTGACGGGGTGCATTGTGTTTCTACTGATCTCTCTTAGGTTTTTACGGAAT1080.10131902358481715No Hit
ACTAGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1070.10038088447755034No Hit
ACATGGGGACTCTTTTAGAGGACGAATCCCTGCAGCTGGGAGAGAGGAAGCG1070.10038088447755034No Hit
AACTATGGGCATACAGCAGCTCTATTGAATAACATGCATCTGAATTTTGAGT1070.10038088447755034No Hit
CTTTCCGGGGGATATTTTTTAAAATTTTAACTTGTTAATGGATAATACATTT1070.10038088447755034No Hit
GACTAGGGGAAGTAAAGATCTTGTGTTGTAATCTAAATTCTTCTCATAATGA1070.10038088447755034No Hit
ATCTACGGGAGAGTCAACTACCGAAGGAAGACAATTTTCGTTTGGTCTAAAA1070.10038088447755034No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)