FastQCFastQC Report
Sat 31 Dec 2022
SRR5845457.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845457.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences107436
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT3950.3676607468632488Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC3590.3341524256301426No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC1510.1405487918388622No Hit
AAAACAGGGCCACCTGTTAACATTTCCATATAAGTGAATCCTGCTTTTGGTG1470.13682564503518374No Hit
TCACTGGGAGGCGCGGTGAAACCATCGAGACGGAGGGCCAGAGAGTCACGGC1390.1293793514278268No Hit
CGTTGAGGGGGAAGTTATGACTTTAAAGAATAAAAACCTAAGCAAATGCTTG1390.1293793514278268No Hit
CAAAAGGGGGCATCCCCCTGCCCTCTCCTAATACTAATCAGGGGATCCTTCA1360.12658699132506795No Hit
TAACGCGGGATGTGAATCCAGAGATAAGTTTATACGGGAATTATTTGAATCA1360.12658699132506795No Hit
TTATAAGGGACTTTATTCAAAGAGCAGCCCGCTTTGTGTGACTAAAATGAAA1340.12472541792322872No Hit
TCATCCGGGGTTGAAACTATAATGGGATAAGGAAACATGTAATCATGGAGAA1290.12007148441863062No Hit
CCATAAGGGGATCTGTATCCGGGCTCTGAAATGAAATACGTCTCTTCACCAT1280.11914069771771102No Hit
AGTATGGGATTAAATCAGAGTCTTTAATTCTTGCATGTTTGTATCTAATTTC1280.11914069771771102No Hit
GGTAACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1260.11727912431587179No Hit
CGCTGGGGGCACAAGTTGCCAGTTCCAGGATATAATTGAAGTCAGCAACTAA1250.11634833761495215No Hit
TGCAGTGGGTTGGCATTTACAAGAGATGTTGGGTGTCAAAGTAAACATTGTA1250.11634833761495215No Hit
CTACTCGGGGATATTAAATTATTCTGTGTTTTGCTTTTCCTTGATATGTAGG1250.11634833761495215No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTA1250.11634833761495215No Hit
TTTCTGGGGGTCTTACTTTACCAATAACGTGTTTTTCATTTTAATCTTTAGT1250.11634833761495215No Hit
GAATCTGGGAATCTTTGCCTCCCCTGACAAAGACAGCTATGACCCTGACTTC1230.11448676421311292No Hit
TAATATGGGGACACCATTTATTAAATGTTATGTTCCTTCTCCAACAGTTTTG1220.11355597751219329No Hit
CGCAGTGGGAAGCCTTGTAAGTCCTGTTCTTCATTCAAGTGGTGTCTAGCTA1220.11355597751219329No Hit
GCTCGCGGGTACTCCATGCAGGGACTCTGCAAGTTTGGCTTTTATGAAGTCT1220.11355597751219329No Hit
TAGGTTGGGGGCTACAGAAGCCTCCGTGAAGAACAATGTAGAAGTGTTTGTA1210.11262519081127369No Hit
GGCGATGGGGAAGGTGTAAGGAGGGGCTTGCTAAGAATTTGAGCACATGTAG1210.11262519081127369No Hit
TTAAGCGGGGGCAGCAAGGATGTGGTCCGCTACCTGCTGGACCACGCCCCCC1200.11169440411035406No Hit
TCAGATGGGCTGATTTGAAATGCCATCTTCGTCTGATGCTGTTTACATGTCT1200.11169440411035406No Hit
TTTGCAGGGTAAACAATCTATAGCACCATAAATTAGCATTTTCTAGTGTGAC1190.11076361740943445No Hit
CTTCCAGGGATGCAATAGAGATTTCATAGTTTGCTTTCTTTGCTCTTTAAAT1180.10983283070851484No Hit
ATCTACGGGAGAGTCAACTACCGAAGGAAGACAATTTTCGTTTGGTCTAAAA1180.10983283070851484No Hit
CCATGGCAACAAAAAGTCTACTCTTACTTTGCTGTTTATTTTTATCTTCAAT1170.10890204400759522No Hit
TGACAGCGGGTGCTCAGATGCTACTGTGAGCTTCCTGTCCGGTGTTAGAAAG1170.10890204400759522No Hit
GTCGCAGGGAGAAGCTGAGCTGCAGAGATTACTCCTCTCATTAAATAAAGGA1160.1079712573066756No Hit
GGTTAGGGATTTCAAAATGCCACCTCTTCTGATTCCAGATACAATTATCTTT1160.1079712573066756No Hit
GACATAGGGAAAGTATATGAATCCAATTAAAGTTGTTCTGTGAGTGTGTTTT1150.10704047060575599No Hit
ACACAGGGGACATGTATCACTGGAGTTGCTTCAAACACAAAGAACATTGCCC1150.10704047060575599No Hit
CTACATGGGGAGTGTGCCATGGACATTGGGATTTTAAAAGTTCCCAGCCCAG1140.10610968390483637No Hit
CTTGACGGGGTGCATTGTGTTTCTACTGATCTCTCTTAGGTTTTTACGGAAT1140.10610968390483637No Hit
CGTGGGGGGTGATGCAGTGATAAAGCGGAAACATTGCTCTGCTACCTGCCCA1130.10517889720391677No Hit
TCTACCGGGATAGAAGGAGACATCCAAGGGATTTGACCATCTTGAACTCGTT1130.10517889720391677No Hit
GATCATGGGGTAACTGTGAACAAATAATTTGTTCTGTGCCTTTTGCCTGGTA1130.10517889720391677No Hit
TAATTGGGGGGATGGGCTCGGACTCTTAGCTGGCAGGCTAGGACACACAGTG1130.10517889720391677No Hit
TACAAAGGGTCTATACGCGGGAGCCAAACACAGCAGCAAATAAAGGAAATGA1130.10517889720391677No Hit
ATAGCCGGGGAAATCTATCTTTATCCTAGACCTCAAGTTACTTTTAGAAATA1120.10424811050299713No Hit
CGTGCTGGGAATCTAGCTGGAGCTGTTGAATTCAATGATGTGAAGACCTTGC1110.10331732380207753No Hit
TGACTGGGGGAGTGCAGTGGCGCGATCTTGGCCCATTGCAACCTCCGCCTTC1100.1023865371011579No Hit
CATATGGGGTTAAGGGATAATTTTTGAAATATTTATGAAAATTTTTATTAGA1100.1023865371011579No Hit
AAGCTTGGGAAATAATGAAATGAAATTGAAATGAGATAAGATACGAGATGAG1090.10145575040023827No Hit
GCACCTGGGATGGTTGGAAAAAATCAAAAGAAGAACTAGATACTTCAGGACA1090.10145575040023827No Hit
TAGTGTAGGGGTGCCCATCATAGATTTCTTTTTTTAGTGTTGCCCTTGCTGT1090.10145575040023827No Hit
CACGCAAGGGTCTGTGCGTGGGTGGTGATTCTGAAGCTTATATTGGAGCAGA1090.10145575040023827No Hit
ACTAGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1080.10052496369931867No Hit
ACGTACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1080.10052496369931867No Hit
TACTGAGGGGCTAGCCGCCATGGATGCAGCAGCCTGAAACTTGAGAGCGAAA1080.10052496369931867No Hit
TTCTAGGGGATTTGAATTTATATGTTGATGCATTGTTTCCCGTCACTTCCTA1080.10052496369931867No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)