FastQCFastQC Report
Sat 31 Dec 2022
SRR5845456.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845456.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences235827
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC6890.2921633231139776No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT6790.2879229265520911Illumina PCR Primer Index 5 (95% over 21bp)
CGGTCCGGGTGTTTGTCTAATTCTCTAAGGAATCCACAGGTGTCAAAATAAA3290.13950904688606477No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC2770.11745898476425516No Hit
TTCGACTGGGACTTCAATAAACATTTATTTTGGTATTCAGCATCTTTGGTAT2570.10897819164048222No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTA2510.10643395370335035No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)