FastQCFastQC Report
Sat 31 Dec 2022
SRR5845455.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845455.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236550
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC7080.2993024730500951No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT6040.25533713802578734Illumina PCR Primer Index 5 (95% over 21bp)
CGGTCCGGGTGTTTGTCTAATTCTCTAAGGAATCCACAGGTGTCAAAATAAA2930.12386387655886703No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC2710.11456351722680194No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTA2500.1056859015007398No Hit
TTCGACTGGGACTTCAATAAACATTTATTTTGGTATTCAGCATCTTTGGTAT2450.103572183470725No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)