FastQCFastQC Report
Sat 31 Dec 2022
SRR5845454.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845454.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences237471
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC6820.28719296250910636No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT6740.28382413010430746Illumina PCR Primer Index 5 (95% over 21bp)
CGGTCCGGGTGTTTGTCTAATTCTCTAAGGAATCCACAGGTGTCAAAATAAA2950.12422569492695949No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC2940.12380459087635964No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)