FastQCFastQC Report
Sat 31 Dec 2022
SRR5845447.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845447.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121285
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC3610.29764604031825864No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT3400.28033145071525745Illumina PCR Primer Index 5 (95% over 21bp)
GGCCTGGGCGACTAAGTGAGACCCTGTCTAAAAGAAAAGAATAAATAAATGT1780.14676175949210538No Hit
TGTCTCGGGGAAAAAAGCGTGAGTATGAAGCCACTTCTCTCCTCAAAGAAAA1660.1368677082903904No Hit
CAAACAGGGACACAGAGAGGGAGCGAGAGAAAAAACGCTCTCTCTTAAGTGG1550.12779816135548502No Hit
GCAAAAGGGGGTGGTTGAACTAATTTATGTTCCCACCAATAGTGTAGAATGT1540.12697365708867545No Hit
CATTGCGGGGGGGCTGAAAGAGGCGGCTCCGGGCGGCGCGAAGCGCTGGTGG1500.1236756400214371No Hit
GATTATGGGAATGAAAAAGAGTCAGTCAGCAGCAGAATTGATCGAGTGGGCG1480.12202663148781795No Hit
TCACCGGGGGAAAGTGTGAAAACAGAAAGAAACCTAAAACTTGCTTTAGAGA1470.12120212722100837No Hit
GTCTGCGGGGCCTTTTTCCCACCCCCTAGCGCCGCTGGGCCTGCAGGTCTCT1430.11790411015377003No Hit
AGAGACGGGATCTGCAACCGGGGACTTCGAGATAATGGACTTCTGGCCAAGC1390.11460609308653173No Hit
AATGACGGGGAATTCTTTGGGTAACTTCTAAACTCAAGCTTTATTTTTTAAT1390.11460609308653173No Hit
ACGCTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1380.11378158881972214No Hit
TACTTTGGGGAAACAAGACAGTTGAATTGTGTGACTTGAAGATTACCAATGA1380.11378158881972214No Hit
GTTTTAGGGAAATGAAGGAGAAATAAATTCTTTCCCAGGCATGCAAAAGCTG1370.11295708455291255No Hit
GCTATAGGGCTAACTATGCAGACCTAGGTATGGATTTTATTGTACGTGGAAT1370.11295708455291255No Hit
CTTGAGCGGGAGGGATGAGCATTTCTTTGAAAGCAATTAGGTTATTCACCTG1370.11295708455291255No Hit
TCACTAGGGCAATTCTTCCAACTTTCTTAAGCAATGAAACCCTTTATTCCTC1360.11213258028610298No Hit
CCGTCCGGGAAGATAAGAATGTTTTTCTGCACAATCCTGATACCATTATCAA1360.11213258028610298No Hit
CATGTCGGGTAACCACTGGTGGAATTGATGATATGTGTCTGAAGTATTTTGT1340.11048357175248381No Hit
TAGTTTGGGACAAGGGTATTGATATGAAATTATGTAAATTTCAAATGCTTAT1340.11048357175248381No Hit
GGCACAGGGTCTCCAAAAGGGCATTTGGATCATGGAAACAGTATAGAGCAGG1340.11048357175248381No Hit
TTGCGCGGGTCAACCCTGGCTCTTAATTCCCTTTTCCAATATTAGACCCCCA1340.11048357175248381No Hit
AGCAAAGGGAACTTGGAAAGGCTGTCTTTCAAACTTCAGTTCCTATCTGACT1330.10965906748567425No Hit
TCACATGGGGCAAGCTGTTGGCAGATAGCTCTGCAGAAATACCAGGGCCAGG1320.10883456321886466No Hit
TAGTCAGGGACCTTGAGGATTTCCCTTAAAAGTTCTCATGGTTTTTCAGATG1310.10801005895205507No Hit
TCAGGCGGGAGCATGGCTGACCAACTGACTGAAGAGCAGATTGCAGAATTCA1310.10801005895205507No Hit
CTTGCAGGGAATGATGAAAACATAATGTAGGAGCATAGTAATGTGATTGAAT1310.10801005895205507No Hit
GATGCTGGGACTTCAGAGCCTTTAATAAACATCCCAACTACCTCAGCCCATT1300.1071855546852455No Hit
CACTCAGGGATCTATAATCATGAAATATATGGTAAAAGACTGCTGCAGTTAT1300.1071855546852455No Hit
GTTCAAGGGGGGCTGCCCAACACACTGCCTACCGCCTGGCACAACCTGGTAT1300.1071855546852455No Hit
GTGCCGGGGTATATCAGTTTTATTCACAATATTCAAAACCTGAAGACAAACC1300.1071855546852455No Hit
TCTTAAGGGAATGTATAAAGATTGTACTTAGCCAGTTCAAAGTGTATATTTA1290.10636105041843592No Hit
GGCTGTGGGATAAGGGGAGTGTTGATTTAAAAGCCTTAGATTAAAATCTGCA1290.10636105041843592No Hit
TGTAAGGGGTAATTCAGGACAGTACTGGCACCAAGCTCCTTGTTGGCTTGAA1280.10553654615162633No Hit
TCACTAGTGGGCTGTAGTTGGGCCTAAATAAATGAGCTGTTGGAAGAAAAGA1280.10553654615162633No Hit
GTACTAGGGATAAATTGAAATAATCACAAAGATCGTTTTTTGAATTGTCCGG1280.10553654615162633No Hit
ACACTTGGGGGAAGATGCGTTACCAAAGATTCACAGTTTATGGACCAGAAGA1270.10471204188481677No Hit
TGAGTAGGGGGAAATAATGGCAAGAAAAAAGTCTGTACACATTCAGTACAGA1270.10471204188481677No Hit
CTCAACGGGATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACT1270.10471204188481677No Hit
GTTGCAGGGGTATGCAAGAAAGCTCCAAGACAATGATAATGATCTCCATTTA1270.10471204188481677No Hit
TTAAACGGGAGATATCACTTGTATATATAACTGAACTTTTTTCTGTAATACA1270.10471204188481677No Hit
TCGAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1260.10388753761800718No Hit
CTGAAACAGATAATATTATTATTCCCATTTTTTTCAGATGGTAAAATCAGCA1260.10388753761800718No Hit
GCGATTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1260.10388753761800718No Hit
AAAAAAGGGGTGTGAAAATAAGGTGACAAACAGACAATCCCATCTTACACTG1260.10388753761800718No Hit
GCACTACTGCACTTAATATTCTAAAGATTCTAAGGGTTAGCCGACTGTAATT1260.10388753761800718No Hit
TTAAAGGGGAAATACGCTTTCTGTACAAATGTGCTGTCTGGATTTATTGCTT1260.10388753761800718No Hit
ATATGCGGGGAAACCAACAACAAACATTTTTAAAATTAAAATGGATAATTTG1260.10388753761800718No Hit
GAAAGGGGATCTGAGGATTTTAGTATCCTCAGGAGTCCTGGAACCAACCCCC1250.10306303335119758No Hit
AAAAATGGGCACACCAAAGCAAATTGTTTTGATTTTCCTCCACAATGTGTGT1250.10306303335119758No Hit
GCAGGCGGGTCATCTGTGGGTGAATGAGTCTGTTTAATTGGCAAGTTGTCAT1250.10306303335119758No Hit
ATGTTAGGGAGATATTTCTTTCCTGGGGACAAAGTGACCTGCAAATGATTGG1240.102238529084388No Hit
GAGAATGGGGGCCTCCAACTCCTGGGCTCCAGTTGATCCTCCTGCCTCAGTC1240.102238529084388No Hit
CTTTGTGGGACCTGCTTGAAGCCCTTTCCATAGCTCTTGGATGAAGGGCCAA1240.102238529084388No Hit
AGTACTGGGGGGTGGCCGCTGCAGTGGAAGAGAACCCCGATAGTGGTCTAGT1240.102238529084388No Hit
ATCACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1230.10141402481757844No Hit
GGCCAGAGGAGCAAAGCTGCCTTCCAAGTGCCTGTCTGTGCCTGGGAGAACA1230.10141402481757844No Hit
CAAAAGGGATTTTCATGACGCAGCCAGCTACGACTATCGAAGCATTTTCAGA1230.10141402481757844No Hit
GGCTGCAGGGAATAGGGCATGACTTGGCTTATTGAGTATGCCAAGAATATTT1220.10058952055076885No Hit
TACTTTGGGCTTTCCAAATTCAGAAGGCAGATTCATTTCATCAAAAGAAAAC1220.10058952055076885No Hit
AACAAGGGGGACTCGCGTCTGGCTACCAGCTCCCCGCTGCCCTGAGCTCGGC1220.10058952055076885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)