FastQCFastQC Report
Sat 31 Dec 2022
SRR5845446.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845446.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences121288
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC3410.28114900072554583No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT3070.2531165490403008Illumina PCR Primer Index 5 (95% over 21bp)
CATTGCGGGGGGGCTGAAAGAGGCGGCTCCGGGCGGCGCGAAGCGCTGGTGG1710.14098674229932062No Hit
TTAAACGGGAGATATCACTTGTATATATAACTGAACTTTTTTCTGTAATACA1590.13109293582217532No Hit
GTTTTAGGGAAATGAAGGAGAAATAAATTCTTTCCCAGGCATGCAAAAGCTG1520.1253215487105072No Hit
TGAGTAGGGGGAAATAATGGCAAGAAAAAAGTCTGTACACATTCAGTACAGA1510.12449706483741178No Hit
GATTATGGGAATGAAAAAGAGTCAGTCAGCAGCAGAATTGATCGAGTGGGCG1500.12367258096431634No Hit
GCTATAGGGCTAACTATGCAGACCTAGGTATGGATTTTATTGTACGTGGAAT1490.12284809709122091No Hit
CAAACAGGGACACAGAGAGGGAGCGAGAGAAAAAACGCTCTCTCTTAAGTGG1470.12119912934503001No Hit
GCAGGCGGGTCATCTGTGGGTGAATGAGTCTGTTTAATTGGCAAGTTGTCAT1460.12037464547193456No Hit
GGCCTGGGCGACTAAGTGAGACCCTGTCTAAAAGAAAAGAATAAATAAATGT1450.11955016159883912No Hit
GGCTGCAGGGAATAGGGCATGACTTGGCTTATTGAGTATGCCAAGAATATTT1440.11872567772574369No Hit
TAGTCAGGGACCTTGAGGATTTCCCTTAAAAGTTCTCATGGTTTTTCAGATG1440.11872567772574369No Hit
GTGCCGGGGTATATCAGTTTTATTCACAATATTCAAAACCTGAAGACAAACC1440.11872567772574369No Hit
AATGACGGGGAATTCTTTGGGTAACTTCTAAACTCAAGCTTTATTTTTTAAT1430.11790119385264825No Hit
TCACATGGGGCAAGCTGTTGGCAGATAGCTCTGCAGAAATACCAGGGCCAGG1430.11790119385264825No Hit
AAGCCAGGGACAAAGATAAGCAAATGATTGCAGAAGATCTAGATCACAAAGG1420.1170767099795528No Hit
TACTTTGGGGAAACAAGACAGTTGAATTGTGTGACTTGAAGATTACCAATGA1420.1170767099795528No Hit
CCGTCCGGGAAGATAAGAATGTTTTTCTGCACAATCCTGATACCATTATCAA1420.1170767099795528No Hit
GTCTGCGGGGCCTTTTTCCCACCCCCTAGCGCCGCTGGGCCTGCAGGTCTCT1420.1170767099795528No Hit
CTTCATGGGAACTGAATTAAACCCAAAATTATGTTTTCATGGTCTCTTCTCT1390.11460325836026647No Hit
AATGCTGGATATCAGTTTTTCAATGGTTTTCCTAGCCATTTATTCAGGGAAC1390.11460325836026647No Hit
GTTCCAGGTACAACAGGAAGATACAGATCGACCTTCCATATCAAGTAATCGG1380.11377877448717102No Hit
TTAAAGGGGAAATACGCTTTCTGTACAAATGTGCTGTCTGGATTTATTGCTT1380.11377877448717102No Hit
GTTACTGGGCTGGAGTACCTGCTGCGCCTCTTCTCGTCACCCAACAAGCTGC1370.11295429061407558No Hit
CCCGTGGGGATTCAATACAGTGTTTTTGAGATTCATCTGTTTTGTTGCTTAT1360.11212980674098014No Hit
GTACTAGGGATAAATTGAAATAATCACAAAGATCGTTTTTTGAATTGTCCGG1360.11212980674098014No Hit
TCATGAGGGATGGCCATAACACATGATGTCATCAATAACCTACTGAAATTTA1350.1113053228678847No Hit
CTAAGTGGGAGAACTCAACTGTATAAATAGTTTGGTCCCCTTAAATGCCAAT1350.1113053228678847No Hit
GTTATTGGGAATAAAACCAACTTATAACACATTGACTCTTTCTCAGTTTTCC1350.1113053228678847No Hit
GAGAGGGGGAAGTCAAACCTGTGATAGTGTTCCCAATTTTGATGTTAGTAGG1340.11048083899478926No Hit
GTAATAGGGACAACATTATTTGGACAAAACCCTTACTAGAATGTAAATGCTG1340.11048083899478926No Hit
ACGCCCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1340.11048083899478926No Hit
TGTCTCGGGGAAAAAAGCGTGAGTATGAAGCCACTTCTCTCCTCAAAGAAAA1340.11048083899478926No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC1340.11048083899478926No Hit
AGTGTCTGGGTCCCCGCCCTCCAGCCGCCTTTGAGTCGTGCCTGGGTCCTCG1330.10965635512169382No Hit
TGTTTGGGGAACACACTCAGAAATTTTCTCAACACAGTTTCTATGCAGAGAA1330.10965635512169382No Hit
GCACTACTGCACTTAATATTCTAAAGATTCTAAGGGTTAGCCGACTGTAATT1330.10965635512169382No Hit
TACTTTGGGCTTTCCAAATTCAGAAGGCAGATTCATTTCATCAAAAGAAAAC1330.10965635512169382No Hit
TTGCACCGGGGAAGCAATGTTAAATCATTCAGGTTTTATTTTCTGCCCTGAA1320.10883187124859839No Hit
AATCAAGGGGGAGCATTTCACGCACGCTCGCGTGGAGGCGGGGCAGGCTGAG1320.10883187124859839No Hit
GCTGCAGGAATCCCAAACTATAGCATTCTCTGCAGCTTTGAAAAAGACTATT1320.10883187124859839No Hit
GGCACAGGGTCTCCAAAAGGGCATTTGGATCATGGAAACAGTATAGAGCAGG1320.10883187124859839No Hit
GTGCCGGGGATCCTGACAAAAGAATCAAGAAATAATAGAATACAAACAAGTA1320.10883187124859839No Hit
GTGATAGGGCTTCTGGTAAATGTTCAGCCTGCTGTAGATGCCTCACTTTTAC1320.10883187124859839No Hit
GCAAAAGGGGGTGGTTGAACTAATTTATGTTCCCACCAATAGTGTAGAATGT1310.10800738737550294No Hit
TAACAAGGGTACTTGCCTCCTTGCTGGCATTGAATTAACACAGGGACAAAAT1310.10800738737550294No Hit
TCAGGCGGGAGCATGGCTGACCAACTGACTGAAGAGCAGATTGCAGAATTCA1310.10800738737550294No Hit
GGCTGTGGGATAAGGGGAGTGTTGATTTAAAAGCCTTAGATTAAAATCTGCA1310.10800738737550294No Hit
AGCAAAGGGAACTTGGAAAGGCTGTCTTTCAAACTTCAGTTCCTATCTGACT1300.10718290350240749No Hit
ACACGTGGGCTAAACGGGAAATTCTCTCAGCTTAAAGGTTCTTCAGATGATG1300.10718290350240749No Hit
ACGCTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1290.10635841962931204No Hit
GTTGCTGGGCATGGGGAGAAATTTAATTCACACCCTGACATTCTTATGCCTC1270.10470945188312117No Hit
CTAGCAAGGGAAGTGGAACAAGAAACTCCTTTACGAGAAGATGAAGGGAGGA1270.10470945188312117No Hit
AAAAAAGGGGTGTGAAAATAAGGTGACAAACAGACAATCCCATCTTACACTG1270.10470945188312117No Hit
TCACTAGTGGGCTGTAGTTGGGCCTAAATAAATGAGCTGTTGGAAGAAAAGA1270.10470945188312117No Hit
ATGGTCGGGACTCAACAAGTCTCACTTTCCCCATGTTCTTAGCCAATTGACT1260.10388496801002572No Hit
CACGCTGGGGAGTTAAAAGTGATGCTCGAAGAGAAAGCCACACTGAGATGCA1240.10223600026383485No Hit
AAGTCAGGGAGATAGATGAAAGCCACAAGAAAGGGAGAGTGTTTCTAAGCCA1240.10223600026383485No Hit
CCAGCAAGGGATTCGGGCGAAGACAAACGGATGCACCCGTCTTTAGAACCAA1230.1014115163907394No Hit
GAAAGGGGATCTGAGGATTTTAGTATCCTCAGGAGTCCTGGAACCAACCCCC1220.10058703251764395No Hit
TGACTGGGACAGCTGGTCCTTGGCTGACTATCCATGTCCAGGGTGACAGACT1220.10058703251764395No Hit
TTACCTGGGAGTATTCTTGGGTGTCCTAGCCAGTGCAGTAAGACAAGTAAAA1220.10058703251764395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)