FastQCFastQC Report
Sat 31 Dec 2022
SRR5845445.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845445.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences123229
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC3540.2872700419544101No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT2970.2410146962159881Illumina PCR Primer Index 5 (95% over 21bp)
GGCCTGGGCGACTAAGTGAGACCCTGTCTAAAAGAAAAGAATAAATAAATGT1750.142012026389892No Hit
CATTGCGGGGGGGCTGAAAGAGGCGGCTCCGGGCGGCGCGAAGCGCTGGTGG1700.13795453992160936No Hit
TGTCTCGGGGAAAAAAGCGTGAGTATGAAGCCACTTCTCTCCTCAAAGAAAA1660.13470855074698326No Hit
GCACTACTGCACTTAATATTCTAAAGATTCTAAGGGTTAGCCGACTGTAATT1500.12172459404847884No Hit
TGAGTAGGGGGAAATAATGGCAAGAAAAAAGTCTGTACACATTCAGTACAGA1450.11766710758019622No Hit
TCAGGCGGGAGCATGGCTGACCAACTGACTGAAGAGCAGATTGCAGAATTCA1440.11685561028653971No Hit
ACGCTTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1420.11523261569922663No Hit
CCGTCCGGGAAGATAAGAATGTTTTTCTGCACAATCCTGATACCATTATCAA1420.11523261569922663No Hit
GCTATAGGGCTAACTATGCAGACCTAGGTATGGATTTTATTGTACGTGGAAT1420.11523261569922663No Hit
TGTAAGGGGTAATTCAGGACAGTACTGGCACCAAGCTCCTTGTTGGCTTGAA1410.11442111840557012No Hit
GTGCCGGGGTATATCAGTTTTATTCACAATATTCAAAACCTGAAGACAAACC1390.11279812381825706No Hit
GTTATTGGGAATAAAACCAACTTATAACACATTGACTCTTTCTCAGTTTTCC1390.11279812381825706No Hit
ATGTTAGGGAGATATTTCTTTCCTGGGGACAAAGTGACCTGCAAATGATTGG1370.11117512923094401No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC1370.11117512923094401No Hit
ACACATGGGGAAATTGTTCGTTTAGTAATAGATGAAATGGGATTTATGAAAA1350.10955213464363096No Hit
AATGACGGGGAATTCTTTGGGTAACTTCTAAACTCAAGCTTTATTTTTTAAT1350.10955213464363096No Hit
GCTTCTGGGGACTTAAATTTTTCACATTTCATTTTCTTTTGGCATTCTGTGG1350.10955213464363096No Hit
GCAGGCGGGTCATCTGTGGGTGAATGAGTCTGTTTAATTGGCAAGTTGTCAT1350.10955213464363096No Hit
TCTTAAGGGAATGTATAAAGATTGTACTTAGCCAGTTCAAAGTGTATATTTA1340.10874063734997444No Hit
GGCTGTGGGATAAGGGGAGTGTTGATTTAAAAGCCTTAGATTAAAATCTGCA1340.10874063734997444No Hit
GATTATGGGAATGAAAAAGAGTCAGTCAGCAGCAGAATTGATCGAGTGGGCG1330.10792914005631792No Hit
TCGAATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1320.10711764276266139No Hit
GGAGTAGGGTACCTTCTGCGGAGGCAGATCCGCACGGTAATTCAGTACCAAA1320.10711764276266139No Hit
CTCAACGGGATCTGCCTACGACAAACAGACCTAAAATCGCTCATTGCATACT1310.10630614546900485No Hit
TTACCTGGGAGTATTCTTGGGTGTCCTAGCCAGTGCAGTAAGACAAGTAAAA1310.10630614546900485No Hit
AGCAAAGGGAACTTGGAAAGGCTGTCTTTCAAACTTCAGTTCCTATCTGACT1300.10549464817534834No Hit
AAAAATGGGCACACCAAAGCAAATTGTTTTGATTTTCCTCCACAATGTGTGT1300.10549464817534834No Hit
ATATGCGGGGAAACCAACAACAAACATTTTTAAAATTAAAATGGATAATTTG1300.10549464817534834No Hit
GGAGGAGGGCTGTGATGCCGTGCGCAACAAGTGCCGCGAGATGCTGACCGCT1290.1046831508816918No Hit
GTTTTAGGGAAATGAAGGAGAAATAAATTCTTTCCCAGGCATGCAAAAGCTG1280.10387165358803528No Hit
AAGCCAGGGACAAAGATAAGCAAATGATTGCAGAAGATCTAGATCACAAAGG1280.10387165358803528No Hit
GCTGCAGGAATCCCAAACTATAGCATTCTCTGCAGCTTTGAAAAAGACTATT1280.10387165358803528No Hit
TTAAACGGGAGATATCACTTGTATATATAACTGAACTTTTTTCTGTAATACA1280.10387165358803528No Hit
GTGATAGGGCTTCTGGTAAATGTTCAGCCTGCTGTAGATGCCTCACTTTTAC1280.10387165358803528No Hit
CTGAAACAGATAATATTATTATTCCCATTTTTTTCAGATGGTAAAATCAGCA1270.10306015629437876No Hit
TACAGCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1270.10306015629437876No Hit
TCACTAGTGGGCTGTAGTTGGGCCTAAATAAATGAGCTGTTGGAAGAAAAGA1270.10306015629437876No Hit
CACTATGGGGTGTTTTGTAAATGAAAAATATCATATAAATGCAGAATACTCT1260.10224865900072223No Hit
ATCGCCGGGTGGGGCTAAAAGTAATAAAACATTAGAAATATCGAGTTATAGA1260.10224865900072223No Hit
CTTAGTGGGGACGCTCTTCCGATCTAACATTGGGGATCTCAAAACGGGAATT1260.10224865900072223No Hit
GCGTCAGGGATTTTGTGAAAAGCTGTATTTATTATAAATACTAGGGCCATGA1260.10224865900072223No Hit
GATGCTGGGACTTCAGAGCCTTTAATAAACATCCCAACTACCTCAGCCCATT1260.10224865900072223No Hit
TACTTTGGGCTTTCCAAATTCAGAAGGCAGATTCATTTCATCAAAAGAAAAC1260.10224865900072223No Hit
GTACTAGGGATAAATTGAAATAATCACAAAGATCGTTTTTTGAATTGTCCGG1260.10224865900072223No Hit
GTTCCAGGTACAACAGGAAGATACAGATCGACCTTCCATATCAAGTAATCGG1250.1014371617070657No Hit
TAGCTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1250.1014371617070657No Hit
GAGAGGGGGAAGTCAAACCTGTGATAGTGTTCCCAATTTTGATGTTAGTAGG1240.10062566441340919No Hit
GAAAGGGGATCTGAGGATTTTAGTATCCTCAGGAGTCCTGGAACCAACCCCC1240.10062566441340919No Hit
AGTGTCTGGGTCCCCGCCCTCCAGCCGCCTTTGAGTCGTGCCTGGGTCCTCG1240.10062566441340919No Hit
AAAAAAGGGGTGTGAAAATAAGGTGACAAACAGACAATCCCATCTTACACTG1240.10062566441340919No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)