FastQCFastQC Report
Sat 31 Dec 2022
SRR5845420.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845420.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences135609
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT7680.5663340928699422Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC7650.5641218503196691No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC2180.16075629198652006No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTA1970.14527059413460758No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)