FastQCFastQC Report
Sat 31 Dec 2022
SRR5845419.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845419.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences134926
Sequences flagged as poor quality0
Sequence length52
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC7950.5892118642811616No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT7830.5803181002920119Illumina PCR Primer Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC2280.16898151579384255No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTA1700.12599498984628613No Hit
GCTTTTGGGAAGTTTAACATAATTGGAGTCCCGATAGGATAGAACAAAGAAT1430.10598402087069948No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)