FastQCFastQC Report
Sat 31 Dec 2022
SRR5845414.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845414.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences110754
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC4700.42436390559257453No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT4640.4189464940318182Illumina PCR Primer Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC1450.1309207793849432No Hit
TAATCAGGGAGAGTCCTCCTTGTTTATTTGGAAGTCTCGAAAACAGTAGTCA1200.1083482312151254No Hit
AGAACTGGGGTAAAAGAAAATCTTAACAATATCAAAAAACTACTTGTGTATA1190.1074453292883327No Hit
TGTTATGGGAGGATGGGAAAAAATGAAAATAGGAAAAAAGGAAGTGAAAGAG1150.10383372158116186No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTA1110.10022211387399102No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)