FastQCFastQC Report
Sat 31 Dec 2022
SRR5845409.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845409.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences162073
Sequences flagged as poor quality0
Sequence length52
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT5680.3504593609052711Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC4890.30171589345541827No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC1900.11723112424648152No Hit
TCAGCGGGAATGGCGCAACACTTTGTAATACAAATGAAAGTTTTCAAAACTG1830.1129120828268743No Hit
TTCCTGAGGGTTTAATAAATCATTAAATTATTTCATTAAAAAAGAAAAACAG1680.10365699407057313No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)