FastQCFastQC Report
Sat 31 Dec 2022
SRR5845408.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845408.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124694
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC4870.3905560812869906No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT4840.3881501916692062Illumina PCR Primer Index 5 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC1670.13392785538999472No Hit
AGCTAGGGGACTCGTGTCTCGCTACCAGCTCCCCGCTGCCCTGCGCTCGGCG1590.1275121497425698No Hit
GCTGAGGCATGACAATTGCTTGAGCCCGGGAAGTAGAGGTTGCAGTGAGCCG1570.12590822333071358No Hit
TCTTCAGGGCAAAATTCTCAGCTATACAAAAACTTGGAAAATATTTTCCTTA1480.11869055447736059No Hit
TCATTCGGGACCTGGAAATTGTTCCCAGCCTTTGTCTCCTACCTACTAAATT1460.11708662806550435No Hit
AGACCTGGGATGCTGTTAATTCTAGGTACTCAAATTCTGGCTCTTAATCCTG1440.11548270165364814No Hit
TGTAATGGGACACATCTTCGTCTACTGTGGTTCAATGATTACTCTACTGGAT1430.11468073844772002No Hit
GGGGTTGGGATGGATGAATGGGAGGTGCGTTTGGAAGCAAGATGCCACTATT1430.11468073844772002No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTA1410.11307681203586378No Hit
AGTCATGGGTTAAATTTGTGTGTGTCCAACAGTTGAATTGAATGTCTATAAG1410.11307681203586378No Hit
GAGGCAGGGGTAACTGCTCAAACACAGTAAACACACAGTTTTCCAGTTTTGC1400.11227484882993569No Hit
TAACCAGGGAACATGAATGCAATGAGTGCCTTATATCACATTGCCCAGAATG1380.11067092241807946No Hit
TTGTCCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1370.10986895921215134No Hit
AAGCACGGGTCTTGCAGCGGGGAACATCAGAAGTATATCTACATGAAGAATT1350.10826503280029513No Hit
TTAGTTGGGTAAGAGAATGTTGTGGAACTACTTCTGGCCAGACTTCTTAATT1350.10826503280029513No Hit
TCAAACGGGTAATCATCTGTGTCAAAGAGTTGCTAAACAGCAGCCTGAAGAA1340.10746306959436701No Hit
TGCCCAGAGCTCAAATGCAGAAGAGCCTGGGATACAGTCTTGACTTCTGCAA1330.1066611063884389No Hit
TCGACGGGGCTGTCTGCCTGGACTGTCCCCTGTCCCCGCATCTCCCCTGGGA1320.10585914318251077No Hit
CATTCAGGGACTTATATATATTAAATTATAGAAAGTAAGCATGGAATGATTT1320.10585914318251077No Hit
CACACAGGGATGGTGTAAAAAACATTCAAAATAATTTGTCCAAAATCTACCA1320.10585914318251077No Hit
GGGTTAGGGCAGTTATCTTGAACATTCTGACGTGTAAAGGACCATAAGGTAC1310.10505717997658269No Hit
TGAATGGGTCTGCCGATGGGAGTGAGTGTTCCAGATGAAAAAAATAGAGTGC1310.10505717997658269No Hit
GTTAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1300.10425521677065457No Hit
TATCGAGGGAACGGTAATGCAGTGGTAACATTAAGTGCTCAAAGGGAGGGGA1300.10425521677065457No Hit
ATATCTGGGTGTTTACATTGCATTTTAATATATCCATTGGTAATTTGATAAA1290.10345325356472646No Hit
CTATTTGGGGTCACTAGCATTTTTGGTGTGAAAAATACAGAGCTGGCTGTCT1290.10345325356472646No Hit
GAGAATGGGATTTCTCTGTAGCCAAAAGCTGGCAAACTTGACCCAGAGGGAA1290.10345325356472646No Hit
TCGATAGGGGAAATAATGAGTTTGAGTATTTTTTTAATGTCTTCCTCCATAC1280.10265129035879833No Hit
TTGCTTGGGCACCACAAAACAAATGGGCTTGGTTTTGTTCACAGGAAGAGCC1270.10184932715287022No Hit
ACTACTGGGATTCTGAATAATGCTGTTATGAACATGGGAGTGTGGATATCAC1260.10104736394694212No Hit
TATGCGGGGGTCCCCAGGGGAGGTCCCCCTTTCGGGAGGAAGTTTTTAAGGG1260.10104736394694212No Hit
TATGACGGGGATGTTAAGTTTGCCTTCAGTAAGATCTTTTGGCTTTCTGTAG1250.10024540074101401No Hit
CCTTGTGGGCTTTGAATACATGAAGCATGGAGACCTGAATAAGTTCCTCAGG1250.10024540074101401No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)