FastQCFastQC Report
Sat 31 Dec 2022
SRR5845406.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845406.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124134
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC4470.36009473633331723No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT4070.3278714937084119Illumina PCR Primer Index 5 (95% over 21bp)
CTATTTGGGGTCACTAGCATTTTTGGTGTGAAAAATACAGAGCTGGCTGTCT1710.13775436222147036No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC1510.12164274090901768No Hit
TGTAATGGGACACATCTTCGTCTACTGTGGTTCAATGATTACTCTACTGGAT1480.11922599771214976No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTA1420.11439251131841396No Hit
GGGGTTGGGATGGATGAATGGGAGGTGCGTTTGGAAGCAAGATGCCACTATT1410.11358693025279133No Hit
AAGCACGGGTCTTGCAGCGGGGAACATCAGAAGTATATCTACATGAAGAATT1400.11278134918716871No Hit
TGAATGGGTCTGCCGATGGGAGTGAGTGTTCCAGATGAAAAAAATAGAGTGC1400.11278134918716871No Hit
GGCACAGGGATAGAGATTAATACAACTACTTAAAAAATATAGTCAATAGGTT1400.11278134918716871No Hit
GGGTTAGGGCAGTTATCTTGAACATTCTGACGTGTAAAGGACCATAAGGTAC1390.11197576812154607No Hit
GAGGCAGGGGTAACTGCTCAAACACAGTAAACACACAGTTTTCCAGTTTTGC1390.11197576812154607No Hit
TTCGTAGGGTAATTAGAAAGCAGTTAGGGTCAGAGAGAGTTTTTGGGAAATA1360.10955902492467817No Hit
GAGAATGGGATTTCTCTGTAGCCAAAAGCTGGCAAACTTGACCCAGAGGGAA1360.10955902492467817No Hit
AGTCATGGGTTAAATTTGTGTGTGTCCAACAGTTGAATTGAATGTCTATAAG1360.10955902492467817No Hit
ATTGGTGGGCATAGGCACAGCTGTTGGTCAGTATGCCAAATAACATCACACT1320.10633670066218764No Hit
TTTGCAGGGGCTTGAAGACAGACTATTTGAAAATATTCAGTCATAGGACAAA1320.10633670066218764No Hit
GTTTTGGGGGGGCCCCTGATGCCAAGAGCTCTGGAGGTTCTTGAAAGAGGGG1300.10472553853094237No Hit
CATAAAGGGGAAATTAAATCAGTGATAAAATGTCTACCTTCAAAGAAAAGCC1290.10391995746531973No Hit
GGCATGGGGTAACTCTAGAGCCCATTCTCCTAACCACTGAGCCATGATTGTC1280.10311437639969709No Hit
CAATCTGGGTCTGCAGTTGGGATTGGATAGAACTTGAAGACAAACTTCTTTA1280.10311437639969709No Hit
CATATAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1270.10230879533407447No Hit
CATTCAGGGACTTATATATATTAAATTATAGAAAGTAAGCATGGAATGATTT1270.10230879533407447No Hit
CGTCAGGGGGTAGAAGGTGAATAACTATCACCCTCAAGGCAAAAAAGCGAAA1270.10230879533407447No Hit
CCACAAGGGAGCCCAAGGGAATTTGAATCACTTGTTAGTCTCTCACTCTGTT1260.10150321426845184No Hit
TGTTTGGGATAAAGTAAACATGCATACAAATAAAAAACATAAAACAAAAATA1260.10150321426845184No Hit
TCGACGGGGCTGTCTGCCTGGACTGTCCCCTGTCCCCGCATCTCCCCTGGGA1260.10150321426845184No Hit
GTTAACGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1250.10069763320282919No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)