FastQCFastQC Report
Sat 31 Dec 2022
SRR5845405.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845405.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80979
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT5170.6384371256745576Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC4700.5803973869768706No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC1470.18152854443744673No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTA1240.15312611911730203No Hit
CGTTGCGGGTCCAAAAAATAAATGTGGAGGTTATTCAGAACAAAATTCATAG1020.12595858185455489No Hit
TGCACAGGGGGTAGTTATATATCAATACTGAATTTACAGGTAGCCTTCAAAT1010.1247236937971573No Hit
TTCAGCGGGGAGGAAACCCCAGTGGTTACTGTGTTCGGAGAACACCCCGAAA1010.1247236937971573No Hit
TAATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA990.1222539176823621No Hit
AGTTGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC990.1222539176823621No Hit
TTAAATGGGGAGAATTTCAACAAAGAAATAGAAAATATTAAAAATGGAATCA980.1210190296249645No Hit
ACGGAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA980.1210190296249645No Hit
TGGGACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA980.1210190296249645No Hit
AACATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA980.1210190296249645No Hit
GTGCGTGGGATAATTTAATGAGTTTAAGTATATTCAGAGACTTATGCCACCA970.1197841415675669No Hit
GATCTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA960.1185492535101693No Hit
TAAGCCGGGACCCTAACATCACAATTTAAAAAACTAAAGAAGCAAGAGCAAA950.11731436545277171No Hit
AGTGGTGGGTCTTAGTAGAGTTATGAAAGAACGTGATGCCCAGATTGAATTC950.11731436545277171No Hit
TTGTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC940.11607947739537411No Hit
GCACCAAGCGGACCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACA940.11607947739537411No Hit
TTGTGGCGGGCGCTCACGGGGTCGAAGGTCCAAACCATCTTCCCGGGCAGCT940.11607947739537411No Hit
TAAACAGGGATAAGTAGAAATGACATTTATGTCTCAAAAACATTACTCATAT930.1148445893379765No Hit
GTAACAGGGATGCAACGCCAGCTTGCCCTTGAAGTGATCGTCACCCTCTCTG920.11360970128057891No Hit
ATGCCGGGGATCTACAGCCGGGACTGGTCACCAGTTTTTATTTTATTTTTAT910.11237481322318131No Hit
ACTCGGTGGGCATAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCT870.10743526099359092No Hit
CTAATTGGGTCAGAGATGCATCCATGTTGTAGCTCCTGGTGGCTTAGTGCTG870.10743526099359092No Hit
AAATAAGGGAGAAAGAGAGAGAGAAAGGAAGGAAGGAAGGAAAGAGAGAGGA860.10620037293619335No Hit
CATGGCGGGAATCAAATAAATATGTTTATCTCTGTGAAAATTCAGGTATTCT860.10620037293619335No Hit
TTGTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC850.10496548487879574No Hit
CTTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCAGG850.10496548487879574No Hit
TGTGAGAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGG850.10496548487879574No Hit
GAAGCTGGGTATAGTCAATAGGTTACTAAGATATTGCTTAGCGTTAAGTTTT850.10496548487879574No Hit
GGTCTGGGCTTCTTAAATGATGGGAGATCTCAAATTTGAATCCTCCCTGACT840.10373059682139812No Hit
TTGGCCGGGAGGCCAAATCCAGTCTACTTTCTGTTTTTAGAAATAAAGTTTT840.10373059682139812No Hit
AGATCAGGGAATTACAAGTCATGCTAAAGGGAATAAATAACACAGCCTGAAG840.10373059682139812No Hit
GTAATTGGGATCTCAAAAAGTCATAAAAGGGCTAAATTATATGATTCTATAT840.10373059682139812No Hit
GATTAGGGGAACCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAATC840.10373059682139812No Hit
TTGTCTGGGGGCAGCGCGCAGGGACCATGTCGGCGGAGACCGCGAGCGGCCC840.10373059682139812No Hit
GACATGGGGTGATACGGGCATTCTTTATTTTGATAGATGCTGCAGAATTGTC840.10373059682139812No Hit
CTATGTGGGGGTGGATGGTGGGATTTACCTGTTATCTGATATTAATAAAAGG840.10373059682139812No Hit
CTGGTGGGAGCAGTAATATTTTAAGATGGACTGGGAAAAACATCAACTCCTG830.10249570876400055No Hit
GCGACTGGGCAAGAAATAGGTTATAGGTAAAATCTCTGAAGGATCATCTATG820.10126082070660294No Hit
CTGTCAGGGAAAGGATGCACAGACAGATGGAGAGTTGTTCATGATAACTGTG820.10126082070660294No Hit
GTAACTGGGCACACTGCGCATCCTCTTCCGGCCTGTCGCCAGCCAGCTTCCT820.10126082070660294No Hit
GCTAGTGGGGAGCTGGACCGTGCTCAGGAGCGCCTGGCCACTGCCCTGCAAA820.10126082070660294No Hit
GGCACTGGGGGCACAGGGGGGTGCCTTCCCGGAAGGGCTCAGAGGCGGGCTC820.10126082070660294No Hit
GCTAGCGGGATTGTTGTTGGAAGAATGGGAATGATAGCTCAATTAATTTAGT810.10002593264920535No Hit
CATAATGGGTCATGAGAATTCCTAGCCCCGCAGAGCAATTTCATCAAATAAG810.10002593264920535No Hit
AATGGTGGGTATGAAGAGATTTGCAAAAGTTCTCTAGGGAGAATAGATCTCT810.10002593264920535No Hit
GGTTTTGGGCATTGTAGGGAGGTGAGAAGCTACATTTTCTGGAAATAAAATA810.10002593264920535No Hit
GGCTAGGGGATCTGTTTTACAAAGGAGCCCTCATGAATGGTGTAACAGAAAT810.10002593264920535No Hit
GGCACGGGGAGCTATCTATACATCAGATAAAGTAGATTTAAAGGAGGAAAGC810.10002593264920535No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)