FastQCFastQC Report
Sat 31 Dec 2022
SRR5845404.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845404.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81547
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT5020.6155959140127779Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC4980.6106907672875764No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC1440.17658528210725105No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTA1360.1667749886568482No Hit
CGTTGCGGGTCCAAAAAATAAATGTGGAGGTTATTCAGAACAAAATTCATAG1210.14838068843734287No Hit
TGCACAGGGGGTAGTTATATATCAATACTGAATTTACAGGTAGCCTTCAAAT1050.12876010153653722No Hit
CTGCTAGGGGAATAACACATAGAATTAACTTGGAATTTGGACTAGCAAAGAC1050.12876010153653722No Hit
CTTGAAGGGCACCAAAGAAATTAAAAGTAAGGCTGTGCACAGTGGCTCACTC1040.12753381485523685No Hit
GGCTAGGGGATCTGTTTTACAAAGGAGCCCTCATGAATGGTGTAACAGAAAT1010.1238549548113358No Hit
GCGACTGGGCAAGAAATAGGTTATAGGTAAAATCTCTGAAGGATCATCTATG990.1214023814487351No Hit
TGGGACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA990.1214023814487351No Hit
TTCAGCGGGGAGGAAACCCCAGTGGTTACTGTGTTCGGAGAACACCCCGAAA960.11772352140483403No Hit
TGACCGGGAGATGTCAGTTTTGAACCAAGAATTGGTGATTTGTTTATAAGAA950.11649723472353367No Hit
TCTTGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA940.11527094804223333No Hit
TTTGTAGGGTATCTATATAGACATACACACAGGAGTTATTGGATTTAGAGTT940.11527094804223333No Hit
GGCACGGGGAGCTATCTATACATCAGATAAAGTAGATTTAAAGGAGGAAAGC940.11527094804223333No Hit
GCTAGTGGGGAGCTGGACCGTGCTCAGGAGCGCCTGGCCACTGCCCTGCAAA930.11404466136093296No Hit
TAATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA920.1128183746796326No Hit
CATAATGGGTCATGAGAATTCCTAGCCCCGCAGAGCAATTTCATCAAATAAG920.1128183746796326No Hit
TTGTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC910.11159208799833226No Hit
TTGTTAGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC910.11159208799833226No Hit
TTTTTTGGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAG910.11159208799833226No Hit
CAGTTTGGGAATATTTGGAAGTATTTCATTACTAACAATCTCAGTACAACAT910.11159208799833226No Hit
TTATATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC910.11159208799833226No Hit
AGCCTGGGTGACGGAGCCAGATTTTGTCTCTAAAAAACAAGAAAAAGAAAAA910.11159208799833226No Hit
TCGCACAGGGGAATGGGCATGTTAACAGGCCAGAATTCTGACACAGGATGGT910.11159208799833226No Hit
GAAACTGGACATCAGTAACAGCAAGAGAATGGGAAAATAGACTAATACATAG910.11159208799833226No Hit
AATGGTGGGTATGAAGAGATTTGCAAAAGTTCTCTAGGGAGAATAGATCTCT900.1103658013170319No Hit
AAAGTAGGGCCGGCAGATGTGTCTCCTGGTTGAACATGCATTTTTCATGGAG890.10913951463573154No Hit
ACGGAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA890.10913951463573154No Hit
AACAATGGGATTCTCCATGCTACCTATTAAACCATATCCTAAAGTAAGTTCA890.10913951463573154No Hit
TCTTCTGGGAACATTTTGATTTTACTTATTCATTCTTCAGTGCACTTCCTTT890.10913951463573154No Hit
GCTCATGGGAGGTTGGGGATATGACTGACAAGGAAAGGCTGTGGCCACCTGA890.10913951463573154No Hit
GAGTCGGGACACGATGAACCTATTCTGAAGCACTTGAAAGATATTAAAGTGA890.10913951463573154No Hit
CGAAATGGGCATGCACTGAAAAAATCAGAGAAGGCTTTGGATACCCTAAACA880.1079132279544312No Hit
GATCTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA880.1079132279544312No Hit
AGATCAGGGAATTACAAGTCATGCTAAAGGGAATAAATAACACAGCCTGAAG870.10668694127313083No Hit
TAAACAGGGATAAGTAGAAATGACATTTATGTCTCAAAAACATTACTCATAT860.10546065459183047No Hit
GGTCTGGGCTTCTTAAATGATGGGAGATCTCAAATTTGAATCCTCCCTGACT860.10546065459183047No Hit
ATATATGGGAAGCCAACATATGAATATATGTTTTGTCTCGCTATACTGCACT860.10546065459183047No Hit
ATGCCGGGGATCTACAGCCGGGACTGGTCACCAGTTTTTATTTTATTTTTAT860.10546065459183047No Hit
GAATAGGGGGAAAATAATCCTGGTATTCAAAGATTATTTGTGAGTGATATGG850.10423436791053013No Hit
GTAGGCGGGCATAAATGAAGGAGAGATAAAGCCTTTTTTCAGACAAACAAAT850.10423436791053013No Hit
AACATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA840.10300808122922978No Hit
GGTTTTGGGCATTGTAGGGAGGTGAGAAGCTACATTTTCTGGAAATAAAATA840.10300808122922978No Hit
GTGAATGGGCTAGATTAACATAATGTCCTTGGGAAATGATGATAGCCCAATG830.1017817945479294No Hit
TTGTGGCGGGCGCTCACGGGGTCGAAGGTCCAAACCATCTTCCCGGGCAGCT830.1017817945479294No Hit
GTGCGTGGGATAATTTAATGAGTTTAAGTATATTCAGAGACTTATGCCACCA830.1017817945479294No Hit
CATGGCGGGAATCAAATAAATATGTTTATCTCTGTGAAAATTCAGGTATTCT830.1017817945479294No Hit
CGATTTGGGGAAGCAGCTGGAGAAGGACTTCAGCAGCATGAAGAAGTACTGC820.10055550786662908No Hit
GCTCTTGGGAGGCCATTTTGGATCTGCCTCACTGGATTCACAACAGACAGTC820.10055550786662908No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)