FastQCFastQC Report
Sat 31 Dec 2022
SRR5845403.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845403.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81371
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGCCGTCT4910.6034090769438744Illumina PCR Primer Index 5 (95% over 21bp)
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAATCTCGTATGC4370.5370463678706172No Hit
CTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTATACAC1610.197859188162859No Hit
CGTTGCGGGTCCAAAAAATAAATGTGGAGGTTATTCAGAACAAAATTCATAG1120.1376411743741628No Hit
GTAGGCGGGCATAAATGAAGGAGAGATAAAGCCTTTTTTCAGACAAACAAAT1080.13272541814651412No Hit
GGCACGGGGAGCTATCTATACATCAGATAAAGTAGATTTAAAGGAGGAAAGC1060.13026754003268978No Hit
GCACCAAGCGGACCTAATAGACATCTACAGAACTCTCCACCCCAAATCAACA1050.1290386009757776No Hit
TAAACAGGGATAAGTAGAAATGACATTTATGTCTCAAAAACATTACTCATAT1020.1253517838050411No Hit
TGCACAGGGGGTAGTTATATATCAATACTGAATTTACAGGTAGCCTTCAAAT1010.12412284474812894No Hit
TTGTATGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGC1000.12289390569121678No Hit
GGGCCTGGGCGATCTTCGACTGGGTGAAGAAACACCAAAGAAAGCTGTGGAA990.12166496663430461No Hit
TGTCTCTTATACACATCTCCGAGCCCACGAGACTACCAGTAACTGTCTCTTA990.12166496663430461No Hit
ACGGAGGGATGACCCCAATACGCAAAACTAACCCCCTAATAAAATTAATTAA960.11797814946356809No Hit
GTGCGTGGGATAATTTAATGAGTTTAAGTATATTCAGAGACTTATGCCACCA960.11797814946356809No Hit
CTAATTGGGTCAGAGATGCATCCATGTTGTAGCTCCTGGTGGCTTAGTGCTG950.11674921040665594No Hit
AGTAGGGGGGGGGCAGAGCCGGCGCGTCATTGTCGTCATCGTTGCCCGACCG950.11674921040665594No Hit
CTATGTGGGGGTGGATGGTGGGATTTACCTGTTATCTGATATTAATAAAAGG950.11674921040665594No Hit
CATGCAGGGATCTAAATCACACATACATATACATACATACACATATGCACAC890.10937557606518293No Hit
ACCACGGGGGTCTAAACCAGTTTCTTGTTATCATACTGTGGATGTTTCTAGC890.10937557606518293No Hit
ACACGAGGGACCAGATGAATGAATGAGTGAAGCAAGAGAGAATGAATAAACA890.10937557606518293No Hit
TAATCGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA880.10814663700827076No Hit
TGGGACGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA880.10814663700827076No Hit
CATGGCGGGAATCAAATAAATATGTTTATCTCTGTGAAAATTCAGGTATTCT880.10814663700827076No Hit
TCTTGAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA870.1069176979513586No Hit
CTTGAAGGGCACCAAAGAAATTAAAAGTAAGGCTGTGCACAGTGGCTCACTC870.1069176979513586No Hit
AGATCAGGGAATTACAAGTCATGCTAAAGGGAATAAATAACACAGCCTGAAG860.10568875889444643No Hit
TTGTCCAGGGAAATGTTTAATGGTATAGTCTGACCTAGTGAAACAAAATGGA860.10568875889444643No Hit
GCTTCAGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA840.10323088078062208No Hit
CAAGACGGGCACAGAGAAATGCATTGAGTGTGAATGTTGTCATCTGTAATCC840.10323088078062208No Hit
CAACTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA840.10323088078062208No Hit
GCTGGGGGCCATTGGTCAAACCATACTTCAGTGAGAGCCTTTATAAGTGAAA830.10200194172370992No Hit
GTCAGAGGGGTACTATTGAATGATTAAACTAAGTATGAAGTGATACCATTCA830.10200194172370992No Hit
GATCTCGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA830.10200194172370992No Hit
GGGACTGGTTTAGCACAAGCCATCCACATTAATTCAAACCTGTGGCTCTGAA830.10200194172370992No Hit
CGTGGAGGGCAATTTGGTTTATTTTAAAAATGTTTCTACTGTATTTACTACA820.10077300266679776No Hit
CTGCTAGGGGAATAACACATAGAATTAACTTGGAATTTGGACTAGCAAAGAC820.10077300266679776No Hit
TGTCAAGGGTGTTGAGGAATAGAAAATTCTTGTTAGTGCTCTATCATGAAAA820.10077300266679776No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)