FastQCFastQC Report
Sat 31 Dec 2022
SRR5845375.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845375.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139150
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGC4710.3384836507366152No Hit
CTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGCCGTCT3730.26805605461731946No Hit
TTTACTGGGCGATCTAGTGACGGGGAATCATCTAAAAAACACGGTCTCAACC1990.1430111390585699No Hit
CGTTACGGGATTACCATAATAAGCAGCAAGGCTGGAGTGGCAATGCAGGAAT1900.13654329859863457No Hit
ATAGTTGGGTAGATCCCACAAATCAGTGAGAACATACAATTTTTGTCCTTCT1830.13151275601868487No Hit
TTCTGCGGGTGTAGAGAAACCATTTAAGTGTGATGAGTGTGGGAAAGCTTTT1760.12648221343873517No Hit
GTGTTTGGGACGGCCCCGAAAGTAGGAAGCGGAGGGGGAGCAGGTTTGCGGG1760.12648221343873517No Hit
ATTGCAGGGGAAATAAAACAGAGAAACAATAAATTTTAAAGGAATAAAACAT1750.12576356449874238No Hit
TGATAAGGGATCTGTGAAATGGCCTTGATCAACATTTTGAAAATCATATTTC1730.12432626661875673No Hit
TTCCTTGGGGATCTAGAGAGGGGGTGTTCCCTGAATGTGCACTGTTAGCAAA1720.12360761767876392No Hit
GCACAAGGGAACAGGTATAAATGTTAGAAAGGAAGAGGTGAAATTATTATTA1710.12288896873877111No Hit
AAACTGGGGCTACAAGCTGGAGCGCCTAGCCGAGGCAGAGAAGTCCCAACTA1680.12073302191879266No Hit
TTTCGAGGGCTGGTTGGGATATTGTACTGTAGTTTAGCAAGATGTGCCATTG1650.11857707509881424No Hit
CTCTTTGGGGCCCTTTCTCGTTCCCCGGCCATCTTAGCGGCTGCTGTTGGTT1640.1178584261588214No Hit
GCAGCGGGGATCAAATGATTTTCTAAATCTTAGTTATTCTTTCATTGACATG1630.1171397772188286No Hit
GGCCTGGGTGACAGAATGAGTCTTTGTGCCAAATAAATAAATAATCATTCAT1630.1171397772188286No Hit
AGCGCAGGGATTCCCACTTGTTGCTATCTTACCTGTCAAAGAAGCACTGAGT1620.11642112827883579No Hit
AAGAGAGGGATGGTTACAGAGAGCATGATTTTTTCATGGAATTGCAAATTCC1610.11570247933884298No Hit
GCATAGGGGTCTAGTCTGGGGATTCAAATGCATATAAAATACCACCATGTTA1600.11498383039885017No Hit
CAAACGGGGCATTTTAAACCAAATTGCCTTATTTTTCTTCCAAACTTCATAT1600.11498383039885017No Hit
ATGCGTGGGGAGAGCCAAGGCGCGAGGCTCGGAGTGAGAGGTAGAGCTGGAG1590.11426518145885733No Hit
ATCACTGGGAGGTTTTTATATATGACTTGAGTCTGCTGTAATTGGCAGCAGA1590.11426518145885733No Hit
TGCATAGGGACACAAAAGCAGGCAAGATGCTACGTTGGCCCAATTGTCTCTG1580.11354653251886453No Hit
TTAGGTTGGGGTGTTGCATTCAAGAAGACTTTCTCTTACGCTGGGTTTGAAA1580.11354653251886453No Hit
TTCAATGGGGTACTCAATCCCCAATGGACATATATTAGATGCAAGAAACAAA1580.11354653251886453No Hit
TCACACGGGGTCATTCCTCATGTTAAAAAATATGAGACAGTAGCATGGTTGG1580.11354653251886453No Hit
GATCGGGACTACAAAACATTGCTGAAAGAAATTAAAGAAGACCTAAATAAAT1580.11354653251886453No Hit
CGGGTTGGGGTCTTGTTCTTGGTCTTGTCAAACCTTGCCTGATGCTCTTTCT1570.11282788357887172No Hit
CGACTAACGGGATAATGATAAAGAATCAATTCAAGCAGAAGATATAACAATC1570.11282788357887172No Hit
AACTAAACTAAAGAGCTTCTGCACAGCAAAAGAAACTACCATCAGACTGAAC1560.11210923463887891No Hit
CAATTTGGGTAAGAAATATCTCACCCTCTTATTCAATAGTGTTTGAAAACAG1550.11139058569888609No Hit
CTCCAATCGGGAGATGATTTGTCATTAATTCGGAAGAACAGAATGGCTCTCT1540.11067193675889328No Hit
GCTTTAGGGTGGACCTAGTAGACATCTACAGAACTCTCCACCCCAAATCAAG1540.11067193675889328No Hit
AGTGCCGGGATTAAAACATGACCAATGCTTTTGCTTTGTTGTCCCCCAAATT1540.11067193675889328No Hit
ATACCCGGGGATTGCTGTATTTTAGAAAATAGGGGTTAAGACTGATAACAAC1540.11067193675889328No Hit
TAGCGCAGGATCTCAAGGTTTTGTTTTCCTTCCAACACCCTCACTTCATGAA1540.11067193675889328No Hit
AAAGCAGGGATTGGCAAGAATGCTTGTGGCTCTGGCTATGATTTTGACGTGT1530.10995328781890047No Hit
ACATAGGGATCTCGTCCAGGGATTAACTGAGATAGTGAAACATGGTGTCCGG1530.10995328781890047No Hit
TTACCCGGGTGATGTTCCTCTACCAGATTATGCACAAGACCTAAATGTCATT1530.10995328781890047No Hit
TGCTGGGGATAATAAAAAACTGACAAAGCTACCAAAATTTTTAAGTTGTTCG1520.10923463887890766No Hit
CTTGTGGGGAATCTGAAAGACAAATATTATTGACCATTTTATAGCAGGGAAA1520.10923463887890766No Hit
AGTACCGGGGAGAAAATTGTGAAAAAAATCAGAGGTCTACAGATGAAGGCAG1520.10923463887890766No Hit
TCTCATGGGTGACTTGAATGCAACAGCAGGAAAATTTTGCAAGTTACATAAT1510.10851598993891484No Hit
ACGACTGGGGTATTTGAAATTGTTACAGAGCTTTCCTCTTTACTTCAAACAG1500.10779734099892202No Hit
ATGTGCGGGGAATTTGCTTGCAATAAGGTTCTGTGTGCTATTTGTGGGAGAG1500.10779734099892202No Hit
AGAGAAGGGGGGGAATACAAATGATGTCACATTTAGCAGTCATATTCAAAAT1490.10707869205892921No Hit
TGAGATGGGCTTAGCCACCTATGTACATACTGCTTTAAGAAATGTTTTTTTT1480.1063600431189364No Hit
GACAACGGGTCATGATGGGACTGTAAGGCATTAATAACCTGGTGAGTTATTT1480.1063600431189364No Hit
ATAAGTGGGACAATCATGGCAAGCCAACGCCACTTATCCAGTGAACCACTAT1470.1056413941789436No Hit
GAGTACGGGATCATCAGTTGGCTACGTTTTGGTTCTATGCTAAACTGTGAAA1470.1056413941789436No Hit
GTGCATGGGGAGTCCATTTTAGCACAGGTACAGCGCATCGTTAAGGGTGAGG1460.10492274523895079No Hit
TCTAGAGGGTGAGATTTGATGGAGCCCTGAATGTTGACCTGACAGAATTCCA1460.10492274523895079No Hit
TGTTTGGGGATATTACGACACTGAATCTTTGAGTTTTTCCATATGAATAGGT1450.10420409629895795No Hit
TGGTTGGGGAAGTTGAAGCTGCAACATATTTTCAAGTATCAAATCTTTGTAT1450.10420409629895795No Hit
AAATTGGGGAAAGGGCCGGAAGGAAGCCGGGGAGACAGGTGGGGTACTCGGG1440.10348544735896514No Hit
GTGTTCGGGGGGCAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTC1430.10276679841897234No Hit
CTCTGATGGGGTTCCGGAAAAGTCATGGAAGACAGCCCGCTGCCAGACCTCA1430.10276679841897234No Hit
GTGCATGGGGAAAAACTACTCTGTCAAACATAGCAAATAGGAAAGTTAAAAA1430.10276679841897234No Hit
TTGCTAGGGATATGTATGTACTTTGATCAATATGTATAGTCCTGCTCAAGGA1430.10276679841897234No Hit
GCAAAGGGGCAGCCGTGGAAGCCAGCAGTATCGAGCTCTCACAGTGCCGGAA1420.10204814947897953No Hit
AGAGGGGTTGCTCAGGCTGGTCTTGAACTCCTGGACTCAGGCAGTCCTTCTG1420.10204814947897953No Hit
CATTAGGGGAGAATCAAGGATGTGTAATGAGTACTTTGAGTTACATAATGCT1420.10204814947897953No Hit
TTGTTTGGGGGGAGATTTGATAATGACTGTAAATCAATAAGGTACTGTGATC1420.10204814947897953No Hit
TCAGTGGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1420.10204814947897953No Hit
CAAACCGGGAAGAAACCTGTGTATGATTCTATGTATCTATATGCATCCATTA1420.10204814947897953No Hit
GGTTTAGGGAGGGCCACTGACCCCTATGAAGTCAGAAATCCGAGTGGAATAC1420.10204814947897953No Hit
GCTAAGGGGGACTAGGACCCAGCAGAAGCCTCTTTCTCCTCTCTGCCCCCTG1420.10204814947897953No Hit
CATCAAGGGAGTACTGGAAGTTCTAGCCAGAGAAATCAGGCAAGAGAAAGAA1420.10204814947897953No Hit
GTCACTGGGGGACTCAGTGTTCTCTAGGTTTGTGAGCAGTTGTGGTAAGAGA1400.10061085159899388No Hit
AAATGTGGGCACCGTGCCTGGCCAACATTTTCTCCAGATATCTTACTGAGTC1400.10061085159899388No Hit
ATGCTGGGGGACTATGTAGAAAAAAATACACTCAAGTTTGTGTTCTATGTTT1400.10061085159899388No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)