FastQCFastQC Report
Sat 31 Dec 2022
SRR5845374.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845374.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139378
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGC4780.34295225932356616No Hit
CTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGCCGTCT3870.27766218484983285No Hit
TTTACTGGGCGATCTAGTGACGGGGAATCATCTAAAAAACACGGTCTCAACC1970.14134224913544463No Hit
TTCTGCGGGTGTAGAGAAACCATTTAAGTGTGATGAGTGTGGGAAAGCTTTT1890.13560246236852302No Hit
ATAGTTGGGTAGATCCCACAAATCAGTGAGAACATACAATTTTTGTCCTTCT1830.1312976222933318No Hit
GTGTTTGGGACGGCCCCGAAAGTAGGAAGCGGAGGGGGAGCAGGTTTGCGGG1820.13058014894746658No Hit
TTAGGTTGGGGTGTTGCATTCAAGAAGACTTTCTCTTACGCTGGGTTTGAAA1780.1277102555640058No Hit
TCACACGGGGTCATTCCTCATGTTAAAAAATATGAGACAGTAGCATGGTTGG1770.1269927822181406No Hit
AAAGCAGGGATTGGCAAGAATGCTTGTGGCTCTGGCTATGATTTTGACGTGT1740.12484036218054499No Hit
GCTTTAGGGTGGACCTAGTAGACATCTACAGAACTCTCCACCCCAAATCAAG1740.12484036218054499No Hit
CGTTACGGGATTACCATAATAAGCAGCAAGGCTGGAGTGGCAATGCAGGAAT1710.1226879421429494No Hit
ATCCTTGGGATTTAATGTCAACGGAATTATATGTGGATGGTGGGGTGGAGCT1710.1226879421429494No Hit
TTCCTTGGGGATCTAGAGAGGGGGTGTTCCCTGAATGTGCACTGTTAGCAAA1690.121252995451219No Hit
AGAGGGGTTGCTCAGGCTGGTCTTGAACTCCTGGACTCAGGCAGTCCTTCTG1680.12053552210535379No Hit
TTTCGAGGGCTGGTTGGGATATTGTACTGTAGTTTAGCAAGATGTGCCATTG1680.12053552210535379No Hit
ATGAAGGGGATTAAAGTGGGAATCATGTCATGGTAGTGACATTTTGGAGAGT1680.12053552210535379No Hit
CAAACGGGGCATTTTAAACCAAATTGCCTTATTTTTCTTCCAAACTTCATAT1660.11910057541362339No Hit
GATCGGGACTACAAAACATTGCTGAAAGAAATTAAAGAAGACCTAAATAAAT1660.11910057541362339No Hit
TTGAAAGGGGCCACCTAGCATGAACTAACCCAGCTTCCACCTCCATAAAGTT1650.11838310206775818No Hit
ACATAGGGATCTCGTCCAGGGATTAACTGAGATAGTGAAACATGGTGTCCGG1650.11838310206775818No Hit
AGCGCAGGGATTCCCACTTGTTGCTATCTTACCTGTCAAAGAAGCACTGAGT1640.11766562872189297No Hit
TTACCCGGGTGATGTTCCTCTACCAGATTATGCACAAGACCTAAATGTCATT1630.11694815537602779No Hit
GCAGCGGGGATCAAATGATTTTCTAAATCTTAGTTATTCTTTCATTGACATG1620.11623068203016258No Hit
AGAGAAGGGGGGGAATACAAATGATGTCACATTTAGCAGTCATATTCAAAAT1590.11407826199256697No Hit
TTGTTTGGGGGGAGATTTGATAATGACTGTAAATCAATAAGGTACTGTGATC1590.11407826199256697No Hit
ACAGCAGGGGAAATGAGCTTCACAGGCCCAAATAATGACAGAGGAGCAGCAC1580.11336078864670177No Hit
GCAAAGGGGCAGCCGTGGAAGCCAGCAGTATCGAGCTCTCACAGTGCCGGAA1570.11264331530083657No Hit
TTCAATGGGGTACTCAATCCCCAATGGACATATATTAGATGCAAGAAACAAA1570.11264331530083657No Hit
AAGAGAGGGATGGTTACAGAGAGCATGATTTTTTCATGGAATTGCAAATTCC1560.11192584195497136No Hit
ATTGCAGGGGAAATAAAACAGAGAAACAATAAATTTTAAAGGAATAAAACAT1560.11192584195497136No Hit
AAACTGGGGCTACAAGCTGGAGCGCCTAGCCGAGGCAGAGAAGTCCCAACTA1560.11192584195497136No Hit
TAGCGCAGGATCTCAAGGTTTTGTTTTCCTTCCAACACCCTCACTTCATGAA1550.11120836860910618No Hit
CCGAGCTGGGGGACCTGCCCCTATCCTGAAACTGTCTACCACCCTCTTCCCA1540.11049089526324098No Hit
CAATTTGGGTAAGAAATATCTCACCCTCTTATTCAATAGTGTTTGAAAACAG1540.11049089526324098No Hit
CGACTAACGGGATAATGATAAAGAATCAATTCAAGCAGAAGATATAACAATC1540.11049089526324098No Hit
ATGCGTGGGGAGAGCCAAGGCGCGAGGCTCGGAGTGAGAGGTAGAGCTGGAG1520.10905594857151057No Hit
TTGCTTGGGGGCTGGACTCCTGTTCCTCATGACAGGGATATTTTGCACCATT1520.10905594857151057No Hit
ACTTTCGGGTGGTGGTGTGCTTGTCCCACTGGAACTGAAGTCTAAAGAGCCT1520.10905594857151057No Hit
TAGTTAGGGTCTTATGAGGGGATGACCCCAATACGCAAAACTAACCCCCTAA1520.10905594857151057No Hit
TACTAGACATTCCGAGCTGTGACCTCTCACCTCCAGCAACAGCACCAGTTAC1510.10833847522564537No Hit
TATACTGGGGGATAGTTAATGGGTACAAAAAAAGAAAATAGAGTGAATAGAC1510.10833847522564537No Hit
GTGCATGGGGAGTCCATTTTAGCACAGGTACAGCGCATCGTTAAGGGTGAGG1500.10762100187978016No Hit
GCACAAGGGAACAGGTATAAATGTTAGAAAGGAAGAGGTGAAATTATTATTA1500.10762100187978016No Hit
GAGCATGGGGAAGTGTGGTTATTGATTATTGTTTAGTTCTTAGTATTATACT1500.10762100187978016No Hit
AAAAAAGGGGAGAGAAAGGACTACAGAGCCCCGAATTAATACCAATAGAAGG1500.10762100187978016No Hit
CTCCAATCGGGAGATGATTTGTCATTAATTCGGAAGAACAGAATGGCTCTCT1490.10690352853391498No Hit
CTGTCGCGGGACTGGAGAGTTCATCAATCACAGTTTCAGAACATGCTCTTAA1490.10690352853391498No Hit
GGCCTGGGTGACAGAATGAGTCTTTGTGCCAAATAAATAAATAATCATTCAT1490.10690352853391498No Hit
TCACTAGGGATCCTGGCTCTGCCACTTAATAGCTCTATGACACTGGACAAAT1490.10690352853391498No Hit
GTGTTCGGGGGGCAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTC1480.10618605518804977No Hit
TTTTTTGGGATATGATTATTGCCAATTAATACAGACTTCATTTGCATAAAAC1470.10546858184218455No Hit
TGCCACGGGCTAGATTGACAATTAGAACTTGCATTTGCTGTTTTTGTTTAAC1460.10475110849631937No Hit
ATACCCGGGGATTGCTGTATTTTAGAAAATAGGGGTTAAGACTGATAACAAC1460.10475110849631937No Hit
GAATTTGGGGTTCTTCAAGAGCAGGTGTTCTCAGCCTCACATGCCCTGCCGT1450.10403363515045416No Hit
CAAACCGGGAAGAAACCTGTGTATGATTCTATGTATCTATATGCATCCATTA1450.10403363515045416No Hit
CAACAGGGGTATAACATATGGAAAAGACAATAGAATAAATAAACAATGAATA1450.10403363515045416No Hit
CAGGTTGGGAGATGTTCAGAATTAGAGGACCATGTTTGTAAAGCTAAGAGCA1440.10331616180458895No Hit
CCAGAGAGAAGGAAGTCACTATTCATTCATGAATGAATAGTGACTTCCTTCT1440.10331616180458895No Hit
GACAACGGGTCATGATGGGACTGTAAGGCATTAATAACCTGGTGAGTTATTT1440.10331616180458895No Hit
GATGGCGGGAGGCCATGAACCGCATCTGTGTGCTGGACGTGGACCTGCAGGG1440.10331616180458895No Hit
TCTAGAGGGTGAGATTTGATGGAGCCCTGAATGTTGACCTGACAGAATTCCA1440.10331616180458895No Hit
CGGGTTGGGGTCTTGTTCTTGGTCTTGTCAAACCTTGCCTGATGCTCTTTCT1440.10331616180458895No Hit
TGGTTGGGGAAGTTGAAGCTGCAACATATTTTCAAGTATCAAATCTTTGTAT1440.10331616180458895No Hit
ATCACTGGGAGGTTTTTATATATGACTTGAGTCTGCTGTAATTGGCAGCAGA1440.10331616180458895No Hit
TGTTCAGGGTAAGCCTTGATTTGGATTTAGAAAGGAATGGTAATGATTTTTG1440.10331616180458895No Hit
TTGCAAGGGATGTTAATAACCGCAGCACTGATTCTTTAGCTAATGCAAATAT1430.10259868845872376No Hit
TGCAGGGGGAGGCGTTTTTCCCAGCCTGTTATAATCCCTTATCCTTTATTAT1430.10259868845872376No Hit
TGAGATGGGCTTAGCCACCTATGTACATACTGCTTTAAGAAATGTTTTTTTT1430.10259868845872376No Hit
ACGACTGGGAAACTAAACTATCACTTTTCTTAAATGGTACAAATGCCTACAT1430.10259868845872376No Hit
GAGTACGGGATCATCAGTTGGCTACGTTTTGGTTCTATGCTAAACTGTGAAA1430.10259868845872376No Hit
TAAGCAGGGCAATGAAATGTATAAGTATTATAGTTGTATAATTGAGTGACAA1420.10188121511285855No Hit
ACGACTGGGGTATTTGAAATTGTTACAGAGCTTTCCTCTTTACTTCAAACAG1420.10188121511285855No Hit
ATAAGTGGGACAATCATGGCAAGCCAACGCCACTTATCCAGTGAACCACTAT1420.10188121511285855No Hit
TTGCGCGGGACTAACATGTCTGGAGATTAGAAGGTATATGTACATGGGGGAG1410.10116374176699335No Hit
TTCCATGGGAATCTTGTTTAGTGAGGGAGGCATATCAGAAGCTAAAATAGGC1410.10116374176699335No Hit
TAATATGGGTAGCTAATTAAAGCAACAACACATAAAATGATTTCATTAATAA1410.10116374176699335No Hit
GTTATTGGGAGGCATTGAGGCAGCCAGCGCAGGGGCTTCTGCTGAGGGGGCA1400.10044626842112817No Hit
TCGCGCGGGAAAAACAAATGGAAGTTTGTGAAGTATGTGGAGCCTTTTTAAT1400.10044626842112817No Hit
TGCATAGGGACACAAAAGCAGGCAAGATGCTACGTTGGCCCAATTGTCTCTG1400.10044626842112817No Hit
AGTGCCGGGATTAAAACATGACCAATGCTTTTGCTTTGTTGTCCCCCAAATT1400.10044626842112817No Hit
GTGAACGGGATGCAAAATATAGGGATAGAAAAAAGATATTCTTATGCAAATG1400.10044626842112817No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)