FastQCFastQC Report
Sat 31 Dec 2022
SRR5845373.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR5845373.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138146
Sequences flagged as poor quality0
Sequence length52
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTCTCTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGC4430.32067522765769546No Hit
CTTATACACATCTCCGAGCCCACGAGACTGACGTGCATCTCGTATGCCGTCT3410.2468402993933954No Hit
TTTACTGGGCGATCTAGTGACGGGGAATCATCTAAAAAACACGGTCTCAACC1940.14043113807131585No Hit
CGTTACGGGATTACCATAATAAGCAGCAAGGCTGGAGTGGCAATGCAGGAAT1800.1302969322311178No Hit
ATAGTTGGGTAGATCCCACAAATCAGTGAGAACATACAATTTTTGTCCTTCT1790.12957306038538938No Hit
GTGTTTGGGACGGCCCCGAAAGTAGGAAGCGGAGGGGGAGCAGGTTTGCGGG1780.12884918853966093No Hit
ATAAGTGGGACAATCATGGCAAGCCAACGCCACTTATCCAGTGAACCACTAT1760.12740144484820407No Hit
TTCAATGGGGTACTCAATCCCCAATGGACATATATTAGATGCAAGAAACAAA1750.12667757300247565No Hit
AAGAGAGGGATGGTTACAGAGAGCATGATTTTTTCATGGAATTGCAAATTCC1740.1259537011567472No Hit
GATCGGGACTACAAAACATTGCTGAAAGAAATTAAAGAAGACCTAAATAAAT1730.12522982931101878No Hit
AAACTGGGGCTACAAGCTGGAGCGCCTAGCCGAGGCAGAGAAGTCCCAACTA1720.12450595746529033No Hit
AGCGCAGGGATTCCCACTTGTTGCTATCTTACCTGTCAAAGAAGCACTGAGT1700.12305821377383348No Hit
GGCCTGGGTGACAGAATGAGTCTTTGTGCCAAATAAATAAATAATCATTCAT1700.12305821377383348No Hit
ATTGCAGGGGAAATAAAACAGAGAAACAATAAATTTTAAAGGAATAAAACAT1680.12161047008237662No Hit
CAAACGGGGCATTTTAAACCAAATTGCCTTATTTTTCTTCCAAACTTCATAT1680.12161047008237662No Hit
TTACCCGGGTGATGTTCCTCTACCAGATTATGCACAAGACCTAAATGTCATT1670.1208865982366482No Hit
TGCTGGGGATAATAAAAAACTGACAAAGCTACCAAAATTTTTAAGTTGTTCG1660.12016272639091975No Hit
CGGGTTGGGGTCTTGTTCTTGGTCTTGTCAAACCTTGCCTGATGCTCTTTCT1640.11871498269946287No Hit
ACGACTGGGAAACTAAACTATCACTTTTCTTAAATGGTACAAATGCCTACAT1630.11799111085373445No Hit
TTCTGCGGGTGTAGAGAAACCATTTAAGTGTGATGAGTGTGGGAAAGCTTTT1620.11726723900800604No Hit
GCAGCGGGGATCAAATGATTTTCTAAATCTTAGTTATTCTTTCATTGACATG1600.11581949531654916No Hit
CTCCAATCGGGAGATGATTTGTCATTAATTCGGAAGAACAGAATGGCTCTCT1600.11581949531654916No Hit
GCTTTAGGGTGGACCTAGTAGACATCTACAGAACTCTCCACCCCAAATCAAG1600.11581949531654916No Hit
TTTCGAGGGCTGGTTGGGATATTGTACTGTAGTTTAGCAAGATGTGCCATTG1580.11437175162509229No Hit
GAAGCAGGGACTCCAGCCCGCCTGCCTTCAGCCTTCCATGAGCTTCACCTGC1580.11437175162509229No Hit
TGAGATGGGCTTAGCCACCTATGTACATACTGCTTTAAGAAATGTTTTTTTT1570.11364787977936387No Hit
TTCCTTGGGGATCTAGAGAGGGGGTGTTCCCTGAATGTGCACTGTTAGCAAA1570.11364787977936387No Hit
TCACACGGGGTCATTCCTCATGTTAAAAAATATGAGACAGTAGCATGGTTGG1570.11364787977936387No Hit
TGTTTGGGGATATTACGACACTGAATCTTTGAGTTTTTCCATATGAATAGGT1540.11147626424217857No Hit
TTGAAAGGGGCCACCTAGCATGAACTAACCCAGCTTCCACCTCCATAAAGTT1540.11147626424217857No Hit
GCATAGGGGTCTAGTCTGGGGATTCAAATGCATATAAAATACCACCATGTTA1540.11147626424217857No Hit
TTAAAAGGGATCTCCTCTTGGGAACGTGCTCATGGTTGCCCAGCTAGTAACT1540.11147626424217857No Hit
TAGCAAGGGATAAGAAACACACGAAAATCTAGAAAACATTTTACATTTTGTT1540.11147626424217857No Hit
AAAGCAGGGATTGGCAAGAATGCTTGTGGCTCTGGCTATGATTTTGACGTGT1520.11002852055072171No Hit
GTGCATGGGGAGTCCATTTTAGCACAGGTACAGCGCATCGTTAAGGGTGAGG1510.10930464870499326No Hit
TTTATGGGGGTATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAA1510.10930464870499326No Hit
ATACCCGGGGATTGCTGTATTTTAGAAAATAGGGGTTAAGACTGATAACAAC1510.10930464870499326No Hit
TAGCGCAGGATCTCAAGGTTTTGTTTTCCTTCCAACACCCTCACTTCATGAA1510.10930464870499326No Hit
AGTATGGGGAATTGCTTTATGGAAGAACACTGCTGTGAACTCAGCCATAAAC1500.10858077685926483No Hit
TGCATAGGGACACAAAAGCAGGCAAGATGCTACGTTGGCCCAATTGTCTCTG1480.10713303316780796No Hit
GTGTTCGGGGGGCAGTGGTGCTGGGAGTGTCGTGGACGCCGTGCCGTTACTC1480.10713303316780796No Hit
CTCTGATGGGGTTCCGGAAAAGTCATGGAAGACAGCCCGCTGCCAGACCTCA1480.10713303316780796No Hit
CGACTAACGGGATAATGATAAAGAATCAATTCAAGCAGAAGATATAACAATC1480.10713303316780796No Hit
GCACAAGGGAACAGGTATAAATGTTAGAAAGGAAGAGGTGAAATTATTATTA1470.10640916132207955No Hit
GGTTTAGGGAGGGCCACTGACCCCTATGAAGTCAGAAATCCGAGTGGAATAC1470.10640916132207955No Hit
TAGTTAGGGTCTTATGAGGGGATGACCCCAATACGCAAAACTAACCCCCTAA1470.10640916132207955No Hit
CTGTCGCGGGACTGGAGAGTTCATCAATCACAGTTTCAGAACATGCTCTTAA1460.10568528947635111No Hit
ATGTGCGGGGAATTTGCTTGCAATAAGGTTCTGTGTGCTATTTGTGGGAGAG1460.10568528947635111No Hit
TTATGGCGGGGTTGTTTGCCTGCTTTGAAGGTATGTGGATGAAGCACTTGAT1460.10568528947635111No Hit
TTCAAAGGGCATTGTAGATTATACAGATATATGATTTCTAACCTTATTACTG1450.10496141763062267No Hit
GTCGAGGGGAGAGTTACAGGACATGATTAATGAAGTAGATGCTGATGGTAAT1450.10496141763062267No Hit
AGTACCGGGGAGAAAATTGTGAAAAAAATCAGAGGTCTACAGATGAAGGCAG1450.10496141763062267No Hit
TCTCCTGGGAAGAGTAGCATGACATATTTAAAGTGCTGAAGGAAAAAAATCT1440.10423754578489425No Hit
TGCCACGGGCTAGATTGACAATTAGAACTTGCATTTGCTGTTTTTGTTTAAC1440.10423754578489425No Hit
GCAGCCGGGAAGTACATTTCTTCGTGATATAAATTACATTTCTTGCCTTTCC1430.1035136739391658No Hit
TTGTTTGGGGGGAGATTTGATAATGACTGTAAATCAATAAGGTACTGTGATC1430.1035136739391658No Hit
CAATTTGGGTAAGAAATATCTCACCCTCTTATTCAATAGTGTTTGAAAACAG1420.10278980209343738No Hit
TAATATGGGTAGCTAATTAAAGCAACAACACATAAAATGATTTCATTAATAA1420.10278980209343738No Hit
AGGCACGGGCGCAGCCCCCGCGCTGGTGCAGCCACCCTCGCTCCCTCTGCTC1410.10206593024770895No Hit
TGATAAGGGATCTGTGAAATGGCCTTGATCAACATTTTGAAAATCATATTTC1400.1013420584019805No Hit
CCGAGCTGGGGGACCTGCCCCTATCCTGAAACTGTCTACCACCCTCTTCCCA1400.1013420584019805No Hit
CATTAGGGGAGAATCAAGGATGTGTAATGAGTACTTTGAGTTACATAATGCT1400.1013420584019805No Hit
CTTTATTTGGGCAGCATTCAGAGCTTTATGTTTCCAGTGGAAATGTACTGTT1400.1013420584019805No Hit
ATGAAGGGGATTAAAGTGGGAATCATGTCATGGTAGTGACATTTTGGAGAGT1400.1013420584019805No Hit
AATTTAGGGACCCAGACTGTGAAGACTACAATAAATCCCTAATGATTCAAAT1390.10061818655625208No Hit
TGTTCAGGGTAAGCCTTGATTTGGATTTAGAAAGGAATGGTAATGATTTTTG1390.10061818655625208No Hit
TTTCCTGGGATGTGTGTTTGAGCAATATGAAATGTGGGCACCCTGAAAAAAG1390.10061818655625208No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)